{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T08:24:04Z","timestamp":1793953444150,"version":"4.8.0"},"reference-count":230,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:00:00Z","timestamp":1762560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"NIAMS","doi-asserted-by":"publisher","award":["R01 AR074395"],"award-info":[{"award-number":["R01 AR074395"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018693","name":"Horizon 2020","doi-asserted-by":"publisher","award":["874790"],"award-info":[{"award-number":["874790"]}],"id":[{"id":"10.13039\/100018693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Materials Today"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.mattod.2025.11.002","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T01:41:37Z","timestamp":1763602897000},"page":"124-147","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Engineering bone tissue with mRNA: from molecular design and delivery to clinical applications"],"prefix":"10.1016","volume":"91","author":[{"given":"Claudia","family":"Del Toro Runzer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martijn","family":"van Griensven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0484-4847","authenticated-orcid":false,"given":"Elizabeth Rosado","family":"Balmayor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mattod.2025.11.002_b0005","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1097\/00003086-199810001-00003","article-title":"The cell and molecular biology of fracture healing","volume":"355","author":"Einhorn","year":"1998","journal-title":"Clin. Orthop. Relat. Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0010","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1186\/1741-7015-9-66","article-title":"Bone regeneration: current concepts and future directions","volume":"9","author":"Dimitriou","year":"2011","journal-title":"BMC Med."},{"key":"10.1016\/j.mattod.2025.11.002_b0015","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1002\/jor.23460","article-title":"Fracture healing physiology and the quest for therapies for delayed healing and nonunion","volume":"35","author":"Kostenuik","year":"2017","journal-title":"J. Orthop. Res."},{"issue":"Suppl. 2","key":"10.1016\/j.mattod.2025.11.002_b0020","doi-asserted-by":"crossref","first-page":"S129","DOI":"10.1007\/s00198-005-1859-7","article-title":"Animal models for fracture treatment in osteoporosis","volume":"16","author":"Egermann","year":"2005","journal-title":"Osteoporos Int."},{"key":"10.1016\/j.mattod.2025.11.002_b0025","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijpharm.2019.118594","article-title":"RNA-based therapy for osteogenesis","volume":"569","author":"Wang","year":"2019","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0030","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3389\/fbioe.2020.00061","article-title":"Reconstruction of large skeletal defects: current clinical therapeutic strategies and future directions using 3D printing","volume":"8","author":"Vidal","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0035","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1302\/0301-620X.83B1.0830003","article-title":"Substitutes for autologous bone graft in orthopaedic trauma","volume":"83","author":"Keating","year":"2001","journal-title":"J. Bone Joint Surg. Br."},{"key":"10.1016\/j.mattod.2025.11.002_b0040","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1177\/0885328216682672","article-title":"Dimensional evaluation of patient-specific 3D printing using calcium phosphate cement for craniofacial bone reconstruction","volume":"31","author":"Bertol","year":"2017","journal-title":"J. Biomater. Appl."},{"key":"10.1016\/j.mattod.2025.11.002_b0045","doi-asserted-by":"crossref","first-page":"3867","DOI":"10.3390\/ma3073867","article-title":"Bioactive glass and glass-ceramic scaffolds for bone tissue engineering","volume":"3","author":"Gerhardt","year":"2010","journal-title":"Materials (Basel)"},{"key":"10.1016\/j.mattod.2025.11.002_b0050","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3389\/fbioe.2018.00105","article-title":"Tissue engineering and cell-based therapies for fractures and bone defects","volume":"6","author":"Perez","year":"2018","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0055","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.ocl.2019.03.001","article-title":"The reamer-irrigator-aspirator in nonunion surgery","volume":"50","author":"Madison","year":"2019","journal-title":"Orthop. Clin. North Am."},{"key":"10.1016\/j.mattod.2025.11.002_b0060","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1586\/17434440.3.1.49","article-title":"Bone graft substitutes","volume":"3","author":"Laurencin","year":"2006","journal-title":"Expert Rev. Med. Devices"},{"key":"10.1016\/j.mattod.2025.11.002_b0065","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3389\/fimmu.2016.00021","article-title":"Immunogenicity to biotherapeutics\u2013the role of anti-drug immune complexes","volume":"7","author":"Krishna","year":"2016","journal-title":"Front. Immunol."},{"key":"10.1016\/j.mattod.2025.11.002_b0070","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1016\/j.spinee.2013.08.060","article-title":"Complications with the use of bone morphogenetic protein 2 (BMP-2) in spine surgery","volume":"14","author":"Tannoury","year":"2014","journal-title":"Spine J."},{"key":"10.1016\/j.mattod.2025.11.002_b0075","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.spinee.2011.04.023","article-title":"A critical review of recombinant human bone morphogenetic protein-2 trials in spinal surgery: emerging safety concerns and lessons learned","volume":"11","author":"Carragee","year":"2011","journal-title":"Spine J."},{"key":"10.1016\/j.mattod.2025.11.002_b0080","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1002\/sctm.18-0259","article-title":"Concise review: application of chemically modified mrna in cell fate conversion and tissue engineering","volume":"8","author":"Badieyan","year":"2019","journal-title":"Stem Cells Transl. Med."},{"key":"10.1016\/j.mattod.2025.11.002_b0085","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1080\/15476286.2016.1208323","article-title":"The notorious R.N.A. in the spotlight - drug or target for the treatment of disease","volume":"14","author":"Reautschnig","year":"2017","journal-title":"RNA Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0090","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3389\/fbioe.2015.00009","article-title":"Gene therapy for bone engineering","volume":"3","author":"Balmayor","year":"2015","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0095","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.molmed.2013.09.002","article-title":"Challenges and advances towards the rational design of mRNA vaccines","volume":"19","author":"Pollard","year":"2013","journal-title":"Trends Mol. Med."},{"key":"10.1016\/j.mattod.2025.11.002_b0100","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1126\/science.aad8711","article-title":"Messenger RNA modifications: form, distribution, and function","volume":"352","author":"Gilbert","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.mattod.2025.11.002_b0105","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.injury.2006.08.055","article-title":"Health economics: a cost analysis of treatment of persistent fracture non-unions using bone morphogenetic protein-7","volume":"38","author":"Dahabreh","year":"2007","journal-title":"Injury"},{"key":"10.1016\/j.mattod.2025.11.002_b0110","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.3171\/foc.2002.13.6.4","article-title":"Bone morphogenetic proteins and spinal fusion","volume":"13","author":"Walker","year":"2002","journal-title":"Neurosurg. Focus"},{"key":"10.1016\/j.mattod.2025.11.002_b0115","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1089\/ten.tea.2010.0555","article-title":"High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo","volume":"17","author":"Zara","year":"2011","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.mattod.2025.11.002_b0120","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.injury.2010.11.016","article-title":"Bone morphogenetic proteins in orthopaedic trauma surgery","volume":"42","author":"Argintar","year":"2011","journal-title":"Injury"},{"key":"10.1016\/j.mattod.2025.11.002_b0125","doi-asserted-by":"crossref","DOI":"10.1038\/natrevmats.2017.56","article-title":"Tools for translation: non-viral materials for therapeutic mRNA delivery","volume":"2","author":"Hajj","year":"2017","journal-title":"Nat. Rev. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0130","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1038\/nrrheum.2015.28","article-title":"Gene therapy approaches to regenerating the musculoskeletal system","volume":"11","author":"Evans","year":"2015","journal-title":"Nat. Rev. Rheumatol."},{"key":"10.1016\/j.mattod.2025.11.002_b0135","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s13568-020-0964-5","article-title":"Human bone morphogenetic protein-2 (hBMP-2) characterization by physical-chemical, immunological and biological assays","volume":"10","author":"Suzuki","year":"2020","journal-title":"AMB Express"},{"key":"10.1016\/j.mattod.2025.11.002_b0140","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.febslet.2013.11.035","article-title":"Recent advances in mammalian protein production","volume":"588","author":"Bandaranayake","year":"2014","journal-title":"FEBS Lett."},{"key":"10.1016\/j.mattod.2025.11.002_b0145","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.cels.2016.11.007","article-title":"CHO Cells can make more protein","volume":"3","author":"Harcum","year":"2016","journal-title":"Cell Syst."},{"key":"10.1016\/j.mattod.2025.11.002_b0150","doi-asserted-by":"crossref","DOI":"10.1002\/adtp.201800038","article-title":"Controlled non\u2010viral gene delivery in cartilage and bone repair: current strategies and future directions","volume":"1","author":"Gonzalez-Fernandez","year":"2018","journal-title":"Advanced Therapeutics"},{"key":"10.1016\/j.mattod.2025.11.002_b0155","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.gene.2015.12.008","article-title":"Pros and cons of pDNA and mRNA transfection to study mRNA translation in mammalian cells","volume":"578","author":"Andreev","year":"2016","journal-title":"Gene"},{"key":"10.1016\/j.mattod.2025.11.002_b0160","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/7355_2016_30","article-title":"Messenger RNA as a Novel Therapeutic Approach","author":"Stanton","year":"2017","journal-title":"RNA Therapeutics"},{"key":"10.1016\/j.mattod.2025.11.002_b0165","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/nrd4278","article-title":"mRNA-based therapeutics\u2013developing a new class of drugs","volume":"13","author":"Sahin","year":"2014","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.mattod.2025.11.002_b0170","doi-asserted-by":"crossref","DOI":"10.3390\/ijms20225805","article-title":"Current and Future concepts for the treatment of impaired fracture healing","volume":"20","author":"Schlickewei","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0175","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.biomaterials.2016.02.018","article-title":"Chemically modified RNA induces osteogenesis of stem cells and human tissue explants as well as accelerates bone healing in rats","volume":"87","author":"Balmayor","year":"2016","journal-title":"Biomaterials"},{"key":"10.1016\/j.mattod.2025.11.002_b0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.msec.2021.112466","article-title":"FDA-approved bone grafts and bone graft substitute devices in bone regeneration","volume":"130","author":"Gillman","year":"2021","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"10.1016\/j.mattod.2025.11.002_b0185","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/ten.teb.2015.0357","article-title":"A review of the clinical side effects of bone morphogenetic protein-2","volume":"22","author":"James","year":"2016","journal-title":"Tissue Eng. B Rev."},{"key":"10.1016\/j.mattod.2025.11.002_b0190","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1177\/1071100715576370","article-title":"Prospective randomized controlled trial of hindfoot and ankle fusions treated with rhPDGF-BB in combination with a \u03b2-TCP-collagen matrix","volume":"36","author":"Daniels","year":"2015","journal-title":"Foot Ankle Int."},{"key":"10.1016\/j.mattod.2025.11.002_b0195","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2021.626315","article-title":"Systematic review of the preclinical technology readiness of orthopedic gene therapy and outlook for clinical translation","volume":"9","author":"Wilkinson","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0200","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1038\/s41467-019-08852-4","article-title":"Intradermal delivery of modified mRNA encoding VEGF-A in patients with type 2 diabetes","volume":"10","author":"Gan","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mattod.2025.11.002_b0205","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1208\/s12248-016-0034-8","article-title":"A Comparative study of the bone regenerative effect of chemically modified RNA encoding BMP-2 or BMP-9","volume":"19","author":"Khorsand","year":"2017","journal-title":"AAPS J."},{"key":"10.1016\/j.mattod.2025.11.002_b0210","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/B978-0-12-415853-5.00012-1","article-title":"Parathyroid hormone and parathyroid hormone-related protein","author":"Nissenson","year":"2013","journal-title":"Osteoporosis"},{"key":"10.1016\/j.mattod.2025.11.002_b0215","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/B978-0-12-415853-5.00018-2","article-title":"WNT signaling in skeletal homeostasis and diseases","author":"Gori","year":"2013","journal-title":"Osteoporosis"},{"key":"10.1016\/j.mattod.2025.11.002_b0220","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.actbio.2023.03.028","article-title":"Sustained delivery of a heterodimer bone morphogenetic protein-2\/7 via a collagen hydroxyapatite scaffold accelerates and improves critical femoral defect healing","volume":"162","author":"Liu","year":"2023","journal-title":"Acta Biomater."},{"key":"10.1016\/j.mattod.2025.11.002_b0225","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abl6242","article-title":"Efficient healing of large osseous segmental defects using optimized chemically modified messenger RNA encoding BMP-2","volume":"8","author":"De La Vega","year":"2022","journal-title":"Sci. Adv."},{"key":"10.1016\/j.mattod.2025.11.002_b0230","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jconrel.2015.09.050","article-title":"Chemically modified RNA activated matrices enhance bone regeneration","volume":"218","author":"Elangovan","year":"2015","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0235","article-title":"Synergistic osteogenesis after co\u2010administration of cmRNAs encoding BMP\u20102 and BMP\u20107 utilizing a transcript\u2010activated matrix","author":"Del Toro Runzer","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0240","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s42003-020-01606-9","article-title":"BMP-2 and VEGF-A modRNAs in collagen scaffold synergistically drive bone repair through osteogenic and angiogenic pathways","volume":"4","author":"Geng","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0245","doi-asserted-by":"crossref","DOI":"10.1016\/j.nantod.2023.102074","article-title":"Delivery of m7G methylated Runx2 mRNA by bone-targeted lipid nanoparticle promotes osteoblastic bone formation in senile osteoporosis","volume":"54","author":"Liu","year":"2024","journal-title":"Nano Today"},{"key":"10.1016\/j.mattod.2025.11.002_b0250","doi-asserted-by":"crossref","DOI":"10.1016\/j.compositesb.2023.111162","article-title":"Sustained delivery of chemically modified mRNA encoding amelogenin from self-assembling hydrogels for periodontal regeneration","volume":"271","author":"Pan","year":"2024","journal-title":"Compos. B Eng."},{"key":"10.1016\/j.mattod.2025.11.002_b0255","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.biomaterials.2017.11.034","article-title":"Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine","volume":"156","author":"Kwon","year":"2018","journal-title":"Biomaterials"},{"key":"10.1016\/j.mattod.2025.11.002_b0260","doi-asserted-by":"crossref","first-page":"7511","DOI":"10.1093\/nar\/gkw551","article-title":"mRNA capping: biological functions and applications","volume":"44","author":"Ramanathan","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0265","doi-asserted-by":"crossref","first-page":"21677","DOI":"10.1074\/jbc.272.35.21677","article-title":"eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure","volume":"272","author":"Haghighat","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mattod.2025.11.002_b0270","first-page":"277","article-title":"Cap and cap-binding proteins in the control of gene expression, Wiley Interdiscip Rev","volume":"2","author":"Topisirovic","year":"2011","journal-title":"RNA"},{"key":"10.1016\/j.mattod.2025.11.002_b0275","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1073\/pnas.1515152113","article-title":"Structural basis for m7G recognition and 2'-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I","volume":"113","author":"Devarkar","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mattod.2025.11.002_b0280","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000734","article-title":"Melanoma differentiation-associated gene 5 (MDA5) is involved in the innate immune response to Paramyxoviridae infection in vivo","volume":"6","author":"Gitlin","year":"2010","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.mattod.2025.11.002_b0285","doi-asserted-by":"crossref","first-page":"2819","DOI":"10.3762\/bjoc.13.274","article-title":"Synthetic mRNA capping","volume":"13","author":"Muttach","year":"2017","journal-title":"Beilstein J. Org. Chem."},{"key":"10.1016\/j.mattod.2025.11.002_b0290","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1261\/rna.5430403","article-title":"Novel \u201canti-reverse\u201d cap analogs with superior translational properties","volume":"9","author":"Jemielity","year":"2003","journal-title":"RNA"},{"key":"10.1016\/j.mattod.2025.11.002_b0295","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1517\/14712598.2015.1057563","article-title":"Modified mRNA as an alternative to plasmid DNA (pDNA) for transcript replacement and vaccination therapy","volume":"15","author":"Youn","year":"2015","journal-title":"Expert Opin. Biol. Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0300","doi-asserted-by":"crossref","first-page":"e39","DOI":"10.1002\/cpz1.39","article-title":"Cap 1 messenger RNA synthesis with co\u2010transcriptional cleancap\u00ae analog by in vitro transcription","volume":"1","author":"Henderson","year":"2021","journal-title":"Current Protocols"},{"key":"10.1016\/j.mattod.2025.11.002_b0305","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102456","article-title":"CleanCap M6 inhibits decapping of exogenously delivered IVT mRNA","volume":"36","author":"Mandell","year":"2025","journal-title":"Molecular Therapy Nucleic Acids"},{"key":"10.1016\/j.mattod.2025.11.002_b0310","doi-asserted-by":"crossref","first-page":"9051","DOI":"10.1093\/nar\/gkac722","article-title":"2\u2032-O-Methylation of the second transcribed nucleotide within the mRNA 5\u2032 cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion","volume":"50","author":"Drazkowska","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0315","doi-asserted-by":"crossref","DOI":"10.1126\/science.aav0080","article-title":"Cap-specific terminal N (6)-methylation of RNA by an RNA polymerase II-associated methyltransferase","volume":"363","author":"Akichika","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.mattod.2025.11.002_b0320","first-page":"1","article-title":"Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation","volume":"43","author":"Chen","year":"2024","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0325","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1021\/acschembio.0c00864","article-title":"Structural insights into the interaction of clinically relevant phosphorothioate mRNA cap analogs with translation initiation factor 4E reveal stabilization via electrostatic thio-effect","volume":"16","author":"Warminski","year":"2021","journal-title":"ACS Chem. Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0330","doi-asserted-by":"crossref","unstructured":"R.A. Wesselhoeft, P.S. Kowalski, F.C. Parker-Hale, Y. Huang, N. Bisaria, D.G. Anderson, RNA circularization diminishes immunogenicity and can extend translation duration in vivo, Molecular cell, 74 (2019) 508-520. e504. https:\/\/doi.org\/10.1016\/j.molcel.2019.02.015.","DOI":"10.1016\/j.molcel.2019.02.015"},{"key":"10.1016\/j.mattod.2025.11.002_b0335","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1261\/rna.569407","article-title":"Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome","volume":"13","author":"Beilharz","year":"2007","journal-title":"RNA"},{"key":"10.1016\/j.mattod.2025.11.002_b0340","first-page":"348","article-title":"Control of poly(A) tail length, Wiley Interdiscip Rev","volume":"2","author":"Eckmann","year":"2011","journal-title":"RNA"},{"key":"10.1016\/j.mattod.2025.11.002_b0345","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1261\/rna.069286.118","article-title":"Segmented poly (A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life","volume":"25","author":"Trepotec","year":"2019","journal-title":"RNA"},{"key":"10.1016\/j.mattod.2025.11.002_b0350","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.jconrel.2017.01.045","article-title":"cmRNA\/lipoplex encapsulation in PLGA microspheres enables transfection via calcium phosphate cement (CPC)\/PLGA composites","volume":"249","author":"Utzinger","year":"2017","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0355","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1089\/ten.tea.2018.0112","article-title":"An improved, chemically modified RNA encoding BMP-2 enhances osteogenesis in vitro and in vivo","volume":"25","author":"Zhang","year":"2019","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.mattod.2025.11.002_b0360","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.jconrel.2016.08.037","article-title":"Transcript-activated collagen matrix as sustained mRNA delivery system for bone regeneration","volume":"239","author":"Badieyan","year":"2016","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0365","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/s41587-024-02174-7","article-title":"Branched chemically modified poly (A) tails enhance the translation capacity of mRNA","volume":"43","author":"Chen","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0370","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-3-reviews0004","article-title":"Untranslated regions of mRNAs","volume":"3","author":"Mignone","year":"2002","journal-title":"Genome Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0375","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1128\/MCB.26.6.2419-2429.2006","article-title":"A nucleolin-binding 3' untranslated region element stabilizes beta-globin mRNA in vivo","volume":"26","author":"Jiang","year":"2006","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0380","doi-asserted-by":"crossref","first-page":"734","DOI":"10.3390\/vaccines9070734","article-title":"Detailed dissection and critical evaluation of the Pfizer\/BioNTech and Moderna mRNA vaccines","volume":"9","author":"Xia","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.mattod.2025.11.002_b0385","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102648","article-title":"Evaluation of synthetic mRNA with selected UTR sequences and alternative poly (A) tail, in vitro and in vivo","volume":"36","author":"Medjmedj","year":"2025","journal-title":"Molecular Therapy Nucleic Acids"},{"key":"10.1016\/j.mattod.2025.11.002_b0390","doi-asserted-by":"crossref","first-page":"39149","DOI":"10.1038\/srep39149","article-title":"Human cellular CYBA UTR sequences increase mRNA translation without affecting the half-life of recombinant RNA transcripts","volume":"6","author":"Ferizi","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mattod.2025.11.002_b0395","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/0092-8674(86)90762-2","article-title":"Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes","volume":"44","author":"Kozak","year":"1986","journal-title":"Cell"},{"key":"10.1016\/j.mattod.2025.11.002_b0400","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1038\/nrm.2017.103","article-title":"Functional 5\u2032 UTR mRNA structures in eukaryotic translation regulation and how to find them","volume":"19","author":"Leppek","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0405","doi-asserted-by":"crossref","first-page":"7598","DOI":"10.1093\/nar\/gkr484","article-title":"Unique translation initiation of mRNAs-containing TISU element","volume":"39","author":"Elfakess","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0410","doi-asserted-by":"crossref","first-page":"24075","DOI":"10.1073\/pnas.1908052116","article-title":"mRNA structure regulates protein expression through changes in functional half-life","volume":"116","author":"Mauger","year":"2019","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0415","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1089\/ten.tea.2017.0485","article-title":"Maximizing the Translational Yield of mRNA Therapeutics by Minimizing 5'-UTRs","volume":"25","author":"Trepotec","year":"2019","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.mattod.2025.11.002_b0420","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.pharmthera.2007.01.002","article-title":"Modulation of mRNA stability as a novel therapeutic approach","volume":"114","author":"Eberhardt","year":"2007","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0425","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102485","article-title":"Enhancing mRNA translation efficiency by introducing sequence optimized AU-rich elements in 3\u2032 UTR via HuR anchorage","volume":"36","author":"Ma","year":"2025","journal-title":"Mol. Therapy Nucleic Acids"},{"key":"10.1016\/j.mattod.2025.11.002_b0430","doi-asserted-by":"crossref","first-page":"5284","DOI":"10.1038\/s41467-024-49508-2","article-title":"Optimizing 5\u2019UTRs for mRNA-delivered gene editing using deep learning","volume":"15","author":"Castillo-Hair","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mattod.2025.11.002_b0435","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/s41587-019-0164-5","article-title":"Human 5\u2032 UTR design and variant effect prediction from a massively parallel translation assay","volume":"37","author":"Sample","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0440","doi-asserted-by":"crossref","unstructured":"A.K. Morrow, A. Thornal, E.D. Flynn, E. Hoelzli, M. Shan, G. Garipler, R. Kirchner, A.J. Reddy, S. Tabchouri, A. Gupta, ML-driven design of 3\u2019UTRs for mRNA stability, bioRxiv, (2024) 2024.2010. 2007.616676. https:\/\/doi.org\/10.1101\/2024.10.07.616676.","DOI":"10.1101\/2024.10.07.616676"},{"key":"10.1016\/j.mattod.2025.11.002_b0445","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1038\/nrrheum.2009.278","article-title":"Sensors of the innate immune system: their link to rheumatic diseases","volume":"6","author":"Theofilopoulos","year":"2010","journal-title":"Nat. Rev. Rheumatol."},{"key":"10.1016\/j.mattod.2025.11.002_b0450","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1038\/nri.2016.78","article-title":"Discriminating self from non-self in nucleic acid sensing","volume":"16","author":"Schlee","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.mattod.2025.11.002_b0455","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1126\/science.1093616","article-title":"Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA","volume":"303","author":"Diebold","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.mattod.2025.11.002_b0460","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/35099560","article-title":"Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3","volume":"413","author":"Alexopoulou","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.mattod.2025.11.002_b0465","first-page":"37","article-title":"Nucleic acids and endosomal pattern recognition: how to tell friend from foe?","volume":"3","author":"Diebold","year":"2013","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mattod.2025.11.002_b0470","doi-asserted-by":"crossref","first-page":"8164","DOI":"10.4049\/jimmunol.177.11.8164","article-title":"Oligodeoxynucleotides differentially modulate activation of TLR7 and TLR8 by imidazoquinolines","volume":"177","author":"Gorden","year":"2006","journal-title":"J. Immunol."},{"key":"10.1016\/j.mattod.2025.11.002_b0475","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3390\/genes10020092","article-title":"RNA modifications modulate activation of innate toll-like receptors","volume":"10","author":"Freund","year":"2019","journal-title":"Genes (Basel)"},{"key":"10.1016\/j.mattod.2025.11.002_b0480","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.4161\/15476286.2014.972855","article-title":"RNA-guided isomerization of uridine to pseudouridine\u2013pseudouridylation","volume":"11","author":"Yu","year":"2014","journal-title":"RNA Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0485","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3390\/pharmaceutics7030137","article-title":"Screening of mRNA chemical modification to maximize protein expression with reduced immunogenicity","volume":"7","author":"Uchida","year":"2015","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.mattod.2025.11.002_b0490","doi-asserted-by":"crossref","DOI":"10.1016\/j.yrtph.2020.104648","article-title":"Nonclinical safety assessment of repeated administration and biodistribution of a novel rabies self-amplifying mRNA vaccine in rats","volume":"113","author":"Stokes","year":"2020","journal-title":"Regul. Toxicol. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0495","doi-asserted-by":"crossref","DOI":"10.1126\/science.abj9853","article-title":"Low-dose mRNA-1273 COVID-19 vaccine generates durable memory enhanced by cross-reactive T cells","volume":"374","author":"Mateus","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.mattod.2025.11.002_b0500","doi-asserted-by":"crossref","first-page":"5130","DOI":"10.3390\/ijms21145130","article-title":"Self-amplifying RNA viruses as RNA vaccines","volume":"21","author":"Lundstrom","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0505","doi-asserted-by":"crossref","first-page":"14604","DOI":"10.1073\/pnas.1209367109","article-title":"Nonviral delivery of self-amplifying RNA vaccines","volume":"109","author":"Geall","year":"2012","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0510","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/s41587-022-01393-0","article-title":"Engineering circular RNA for enhanced protein production","volume":"41","author":"Chen","year":"2023","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0515","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jconrel.2022.05.043","article-title":"Circular RNA: an emerging frontier in RNA therapeutic targets, RNA therapeutics, and mRNA vaccines","volume":"348","author":"Liu","year":"2022","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0520","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1093\/bioinformatics\/btv678","article-title":"CDSfold: an algorithm for designing a protein-coding sequence with the most stable secondary structure","volume":"32","author":"Terai","year":"2016","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mattod.2025.11.002_b0525","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/s41586-023-06127-z","article-title":"Algorithm for optimized mRNA design improves stability and immunogenicity","volume":"621","author":"Zhang","year":"2023","journal-title":"Nature"},{"key":"10.1016\/j.mattod.2025.11.002_b0530","first-page":"1","article-title":"Predicting the translation efficiency of messenger RNA in mammalian cells","author":"Zheng","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0535","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102491","article-title":"Reverse-phase chromatography removes double-stranded RNA, fragments, and residual template to decrease immunogenicity and increase cell potency of mRNA and saRNA","volume":"36","author":"Kru\u0161i\u010d","year":"2025","journal-title":"Molecular Therapy Nucleic Acids"},{"key":"10.1016\/j.mattod.2025.11.002_b0540","doi-asserted-by":"crossref","first-page":"e142","DOI":"10.1093\/nar\/gkr695","article-title":"Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA","volume":"39","author":"Karik\u00f3","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0545","doi-asserted-by":"crossref","first-page":"480","DOI":"10.2144\/04363RN02","article-title":"Removal of template DNA from cRNA preparations by combined oligo (dT) affinity chromatography and DNase I digestion","volume":"36","author":"St\u00f6cher","year":"2004","journal-title":"Biotechniques"},{"key":"10.1016\/j.mattod.2025.11.002_b0550","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1016\/j.vaccine.2021.03.038","article-title":"mRNA vaccines manufacturing: challenges and bottlenecks","volume":"39","author":"Rosa","year":"2021","journal-title":"Vaccine"},{"key":"10.1016\/j.mattod.2025.11.002_b0555","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1016\/j.tibtech.2022.03.012","article-title":"Quality by Design for enabling RNA platform production processes","volume":"40","author":"Daniel","year":"2022","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0560","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3390\/pharmaceutics14020328","article-title":"Ribozyme assays to quantify the capping efficiency of in vitro-transcribed mRNA","volume":"14","author":"Vlatkovic","year":"2022","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.mattod.2025.11.002_b0565","article-title":"Capped or uncapped? Techniques to Assess the Quality of mRNA Molecules","volume":"37","author":"Tu","year":"2024","journal-title":"Curr. Opinion Syst. Biol."},{"key":"10.1016\/j.mattod.2025.11.002_b0570","doi-asserted-by":"crossref","DOI":"10.1016\/j.chroma.2025.465724","article-title":"Characterisation and analysis of mRNA critical quality attributes using liquid chromatography based methods","author":"Webb","year":"2025","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.mattod.2025.11.002_b0575","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/j.cell.2009.01.019","article-title":"The many pathways of RNA degradation","volume":"136","author":"Houseley","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.mattod.2025.11.002_b0580","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1146\/annurev.bb.15.060186.001115","article-title":"Electrostatic interactions in membranes and proteins","volume":"15","author":"Honig","year":"1986","journal-title":"Annu. Rev. Biophys. Biophys. Chem."},{"key":"10.1016\/j.mattod.2025.11.002_b0585","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1089\/nat.2013.0463","article-title":"Cellular uptake and intracellular trafficking of oligonucleotides: implications for oligonucleotide pharmacology","volume":"24","author":"Juliano","year":"2014","journal-title":"Nucleic Acid Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0590","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.omtn.2023.05.019","article-title":"Cellular uptake of modified mRNA occurs via caveolae-mediated endocytosis, yielding high protein expression in slow-dividing cells","volume":"32","author":"Del Toro Runzer","year":"2023","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"10.1016\/j.mattod.2025.11.002_b0595","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymeth.2025.06.006","article-title":"Inside the cell: Approaches to evaluating mRNA internalization and trafficking","author":"Del Toro Runzer","year":"2025","journal-title":"Methods"},{"key":"10.1016\/j.mattod.2025.11.002_b0600","first-page":"1","article-title":"Physical methods for gene transfer, advances in genetics","author":"Alsaggar","year":"2015","journal-title":"Elsevier"},{"key":"10.1016\/j.mattod.2025.11.002_b0605","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-800148-6.00001-8","article-title":"Nonviral vectors: we have come a long way","volume":"88","author":"Goodwin","year":"2014","journal-title":"Adv. Genet."},{"key":"10.1016\/j.mattod.2025.11.002_b0610","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1089\/hgtb.2013.113","article-title":"Sonoporation increases therapeutic efficacy of inducible and constitutive BMP2\/7 in vivo gene delivery","volume":"25","author":"Feichtinger","year":"2014","journal-title":"Hum. Gene Ther. Methods"},{"key":"10.1016\/j.mattod.2025.11.002_b0615","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1038\/mt.2016.143","article-title":"Viral and Synthetic RNA Vector Technologies and applications","volume":"24","author":"Schott","year":"2016","journal-title":"Mol. Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0620","doi-asserted-by":"crossref","first-page":"387","DOI":"10.2174\/1566523054546224","article-title":"Altering the tropism of lentiviral vectors through pseudotyping","volume":"5","author":"Cronin","year":"2005","journal-title":"Curr. Gene Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0625","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.3390\/v7052321","article-title":"Alphaviruses in gene therapy","volume":"7","author":"Lundstrom","year":"2015","journal-title":"Viruses"},{"key":"10.1016\/j.mattod.2025.11.002_b0630","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1099\/vir.0.80677-0","article-title":"Heterologous gene expression by infectious and replicon vectors derived from tick-borne encephalitis virus and direct comparison of this flavivirus system with an alphavirus replicon","volume":"86","author":"Gehrke","year":"2005","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.mattod.2025.11.002_b0635","doi-asserted-by":"crossref","first-page":"12225","DOI":"10.1128\/JVI.78.22.12225-12235.2004","article-title":"Analysis of adaptive mutations in Kunjin virus replicon RNA reveals a novel role for the flavivirus nonstructural protein NS2A in inhibition of beta interferon promoter-driven transcription","volume":"78","author":"Liu","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.mattod.2025.11.002_b0640","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.actbio.2020.03.045","article-title":"Co-delivery of NS1 and BMP2 mRNAs to murine pluripotent stem cells leads to enhanced BMP-2 expression and osteogenic differentiation","volume":"108","author":"Wang","year":"2020","journal-title":"Acta Biomater."},{"key":"10.1016\/j.mattod.2025.11.002_b0645","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1007\/s11095-006-9180-5","article-title":"Lipid-based nanoparticles for nucleic acid delivery","volume":"24","author":"Li","year":"2007","journal-title":"Pharm. Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0650","doi-asserted-by":"crossref","DOI":"10.3390\/pharmaceutics12020102","article-title":"Opportunities and challenges in the delivery of mRNA-based Vaccines","volume":"12","author":"Wadhwa","year":"2020","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.mattod.2025.11.002_b0655","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1016\/j.ymthe.2019.02.012","article-title":"Delivering the messenger: advances in technologies for therapeutic mRNA delivery","volume":"27","author":"Kowalski","year":"2019","journal-title":"Mol. Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0660","doi-asserted-by":"crossref","first-page":"3767","DOI":"10.1021\/nn3049494","article-title":"Mechanism of polyplex-and lipoplex-mediated delivery of nucleic acids: real-time visualization of transient membrane destabilization without endosomal lysis","volume":"7","author":"Rehman","year":"2013","journal-title":"ACS Nano"},{"key":"10.1016\/j.mattod.2025.11.002_b0665","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1007\/s13346-013-0158-7","article-title":"Toxicity profiling of several common RNAi-based nanomedicines: a comparative study","volume":"4","author":"Landesman-Milo","year":"2014","journal-title":"Drug Deliv. Transl. Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0670","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.bbrc.2005.03.041","article-title":"Cationic lipids enhance siRNA-mediated interferon response in mice","volume":"330","author":"Ma","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mattod.2025.11.002_b0675","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0005-2736(97)00126-0","article-title":"Toxicity and immunomodulatory activity of liposomal vectors formulated with cationic lipids toward immune effector cells","volume":"1329","author":"Filion","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.mattod.2025.11.002_b0680","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.jconrel.2006.04.014","article-title":"Toxicity of cationic lipids and cationic polymers in gene delivery","volume":"114","author":"Lv","year":"2006","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0685","doi-asserted-by":"crossref","DOI":"10.1002\/adma.201606944","article-title":"Synthesis and biological evaluation of ionizable lipid materials for the in vivo delivery of messenger RNA to B lymphocytes","volume":"29","author":"Fenton","year":"2017","journal-title":"Adv. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0690","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/nbt.1602","article-title":"Rational design of cationic lipids for siRNA delivery","volume":"28","author":"Semple","year":"2010","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0695","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1038\/s41578-021-00358-0","article-title":"Lipid nanoparticles for mRNA delivery","volume":"6","author":"Hou","year":"2021","journal-title":"Nat. Rev. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0700","doi-asserted-by":"crossref","first-page":"9926","DOI":"10.1021\/jacs.2c02706","article-title":"Rational design of bisphosphonate lipid-like materials for mRNA delivery to the bone microenvironment","volume":"144","author":"Xue","year":"2022","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.mattod.2025.11.002_b0705","doi-asserted-by":"crossref","unstructured":"I.C. Yoon, L. Xue, Q. Chen, J. Liu, J. Xu, Z. Siddiqui, D. Kim, B. Chen, Q. Shi, E. Laura Han, Piperazine\u2010Derived Bisphosphonate\u2010Based Ionizable Lipid Nanoparticles Enhance mRNA Delivery to the Bone Microenvironment, Angewandte Chemie International Edition, 64 (2025) e202415389. https:\/\/doi.org\/10.1002\/anie.202415389.","DOI":"10.1002\/anie.202415389"},{"key":"10.1016\/j.mattod.2025.11.002_b0710","doi-asserted-by":"crossref","first-page":"2866","DOI":"10.3390\/molecules25122866","article-title":"Role of lipid-based and polymer-based non-viral vectors in nucleic acid delivery for next-generation gene therapy","volume":"25","author":"Wahane","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.mattod.2025.11.002_b0715","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ejpb.2004.11.011","article-title":"Polyethylenimine-based non-viral gene delivery systems","volume":"60","author":"Lungwitz","year":"2005","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0720","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/jgm.187","article-title":"Different behavior of branched and linear polyethylenimine for gene delivery in vitro and in vivo","volume":"3","author":"Wightman","year":"2001","journal-title":"J. Gene Med."},{"key":"10.1016\/j.mattod.2025.11.002_b0725","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nnano.2014.84","article-title":"In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight","volume":"9","author":"Dahlman","year":"2014","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0730","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1002\/jbm.b.34275","article-title":"A bioactive collagen membrane that enhances bone regeneration","volume":"107","author":"Khorsand","year":"2019","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"10.1016\/j.mattod.2025.11.002_b0735","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jconrel.2004.08.010","article-title":"Recent advances on chitosan-based micro- and nanoparticles in drug delivery","volume":"100","author":"Agnihotri","year":"2004","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0740","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.jconrel.2013.08.005","article-title":"Chitosan-based siRNA delivery systems","volume":"172","author":"Ragelle","year":"2013","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0745","doi-asserted-by":"crossref","first-page":"34081","DOI":"10.1039\/C5RA24891D","article-title":"Effective delivery of bone morphogenetic protein 2 gene using chitosan\u2013polyethylenimine nanoparticle to promote bone formation","volume":"6","author":"Zhao","year":"2016","journal-title":"RSC Adv."},{"key":"10.1016\/j.mattod.2025.11.002_b0750","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.jconrel.2016.12.005","article-title":"Self-aggregating 1.8kDa polyethylenimines with dissolution switch at endosomal acidic pH are delivery carriers for plasmid DNA, mRNA, siRNA and exon-skipping oligonucleotides","volume":"246","author":"Chiper","year":"2017","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0755","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1021\/mp100253e","article-title":"pH-responsive nanoparticles for drug delivery","volume":"7","author":"Gao","year":"2010","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0760","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/1556-276X-9-247","article-title":"Dendrimers: synthesis, applications, and properties","volume":"9","author":"Abbasi","year":"2014","journal-title":"Nanoscale Res. Lett."},{"key":"10.1016\/j.mattod.2025.11.002_b0765","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nrd1775","article-title":"Design and development of polymers for gene delivery","volume":"4","author":"Pack","year":"2005","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.mattod.2025.11.002_b0770","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.apmt.2018.05.002","article-title":"PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy","volume":"12","author":"Abedi-Gaballu","year":"2018","journal-title":"Appl. Mater. Today"},{"key":"10.1016\/j.mattod.2025.11.002_b0775","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s12195-018-0536-9","article-title":"Lipid Polymer Hybrid Nanomaterials for mRNA delivery","volume":"11","author":"Zhao","year":"2018","journal-title":"Cell. Mol. Bioeng."},{"key":"10.1016\/j.mattod.2025.11.002_b0780","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.2147\/IJN.S198353","article-title":"Lipid\u2013polymer hybrid nanoparticles as a next-generation drug delivery platform: state of the art, emerging technologies, and perspectives","volume":"14","author":"Mukherjee","year":"2019","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.mattod.2025.11.002_b0785","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1021\/mp500426r","article-title":"Peptides used in the delivery of small noncoding RNA","volume":"11","author":"Shukla","year":"2014","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0790","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.ijpharm.2017.04.008","article-title":"Polymeric nanoparticles for siRNA delivery: production and applications","volume":"525","author":"Cavallaro","year":"2017","journal-title":"Int. J. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0795","doi-asserted-by":"crossref","first-page":"3410","DOI":"10.1073\/pnas.87.9.3410","article-title":"Transferrin-polycation conjugates as carriers for DNA uptake into cells","volume":"87","author":"Wagner","year":"1990","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0800","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1021\/mp070047s","article-title":"Impact of surface derivatization of poly-L-lysine dendrimers with anionic arylsulfonate or succinate groups on intravenous pharmacokinetics and disposition","volume":"4","author":"Kaminskas","year":"2007","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b0805","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0168-3659(02)00002-0","article-title":"PEG grafted polylysine with fusogenic peptide for gene delivery: high transfection efficiency with low cytotoxicity","volume":"79","author":"Lee","year":"2002","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0810","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1038\/nbt1101","article-title":"Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors","volume":"23","author":"Song","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mattod.2025.11.002_b0815","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.tips.2017.01.003","article-title":"Cell-Penetrating Peptides: from Basic Research to Clinics","volume":"38","author":"Guidotti","year":"2017","journal-title":"Trends Pharmacol. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0820","doi-asserted-by":"crossref","first-page":"13055","DOI":"10.3390\/molecules200713055","article-title":"Cell-type specific penetrating peptides: therapeutic promises and challenges","volume":"20","author":"Zahid","year":"2015","journal-title":"Molecules"},{"key":"10.1016\/j.mattod.2025.11.002_b0825","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.201601412","article-title":"Arginine-Rich Peptide-based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide","volume":"6","author":"Udhayakumar","year":"2017","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0830","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1039\/C8TB03124J","article-title":"Peptide-based gene delivery vectors","volume":"7","author":"Kang","year":"2019","journal-title":"J. Mater. Chem. B"},{"key":"10.1016\/j.mattod.2025.11.002_b0835","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.nantod.2015.06.008","article-title":"15 years on siRNA delivery: beyond the state-of-the-art on inorganic nanoparticles for RNAi therapeutics","volume":"10","author":"Conde","year":"2015","journal-title":"Nano Today"},{"key":"10.1016\/j.mattod.2025.11.002_b0840","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1002\/anie.200703039","article-title":"Inorganic nanoparticles as carriers of nucleic acids into cells","volume":"47","author":"Sokolova","year":"2008","journal-title":"Angew. Chem. Int. Ed."},{"key":"10.1016\/j.mattod.2025.11.002_b0845","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1517\/17425247.2016.1134486","article-title":"Progress and perspective of inorganic nanoparticle-based siRNA delivery systems","volume":"13","author":"Jiang","year":"2016","journal-title":"Expert Opin. Drug Deliv."},{"key":"10.1016\/j.mattod.2025.11.002_b0850","doi-asserted-by":"crossref","first-page":"7475","DOI":"10.1039\/c2nr31775c","article-title":"Capacity of mesoporous bioactive glass nanoparticles to deliver therapeutic molecules","volume":"4","author":"El-Fiqi","year":"2012","journal-title":"Nanoscale"},{"key":"10.1016\/j.mattod.2025.11.002_b0855","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1038\/mt.2014.30","article-title":"Gold nanoparticles for nucleic acid delivery","volume":"22","author":"Ding","year":"2014","journal-title":"Mol. Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b0860","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1533\/9781908818782.331","article-title":"Nanomaterial-assisted light-induced poration and transfection of mammalian cells","author":"Ding","year":"2015","journal-title":"Applications of Nanoscience in Photomedicine"},{"key":"10.1016\/j.mattod.2025.11.002_b0865","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0075369","article-title":"Inhibition of Xenograft tumor growth by gold nanoparticle-DNA oligonucleotide conjugates-assisted delivery of BAX mRNA","volume":"8","author":"Yeom","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.mattod.2025.11.002_b0870","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/s007760050010","article-title":"Role of fracture hematoma and periosteum during fracture healing in rats: interaction of fracture hematoma and the periosteum in the initial step of the healing process","volume":"5","author":"Ozaki","year":"2000","journal-title":"J. Orthop. Sci."},{"key":"10.1016\/j.mattod.2025.11.002_b0875","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.injury.2011.03.031","article-title":"The biology of fracture healing","volume":"42","author":"Marsell","year":"2011","journal-title":"Injury"},{"key":"10.1016\/j.mattod.2025.11.002_b0880","doi-asserted-by":"crossref","DOI":"10.1016\/j.bone.2020.115449","article-title":"BMP gene delivery for skeletal tissue regeneration","volume":"137","author":"Bez","year":"2020","journal-title":"Bone"},{"key":"10.1016\/j.mattod.2025.11.002_b0885","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1006\/bbrc.1999.1975","article-title":"Osteoinduction by bone morphogenetic protein-2 via adenoviral vector under transient immunosuppression","volume":"267","author":"Okubo","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mattod.2025.11.002_b0890","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1016\/j.addr.2012.03.013","article-title":"Gene delivery to bone","volume":"64","author":"Evans","year":"2012","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.mattod.2025.11.002_b0895","doi-asserted-by":"crossref","unstructured":"O. Betz, M. Vrahas, A. Baltzer, J.R. Lieberman, P.D. Robbins, C.H. Evans, Gene Transfer Approaches to Enhancing Bone Healing, Bone Regeneration and Repair, Springer2005, pp. 157-168. https:\/\/doi.org\/10.1385\/1-59259-863-3:157.","DOI":"10.1385\/1-59259-863-3:157"},{"key":"10.1016\/j.mattod.2025.11.002_b0900","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s001670050147","article-title":"A gene therapy approach to accelerating bone healing","volume":"7","author":"Baltzer","year":"1999","journal-title":"Evaluation of Gene Expression in a New Zealand White Rabbit Model, Knee Surg Sports Traumatol Arthrosc"},{"key":"10.1016\/j.mattod.2025.11.002_b0905","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/6530624","article-title":"BMP6-Engineered MSCs Induce Vertebral Bone Repair in a Pig Model: a pilot Study","author":"Pelled","year":"2016","journal-title":"Stem Cells Int."},{"key":"10.1016\/j.mattod.2025.11.002_b0910","doi-asserted-by":"crossref","first-page":"96","DOI":"10.22203\/eCM.v018a09","article-title":"Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage","volume":"18","author":"Evans","year":"2009","journal-title":"Eur. Cell. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b0915","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1182\/blood-2007-08-105809","article-title":"Intrauterine transplantation of human fetal mesenchymal stem cells from first-trimester blood repairs bone and reduces fractures in osteogenesis imperfecta mice","volume":"111","author":"Guillot","year":"2008","journal-title":"Blood"},{"key":"10.1016\/j.mattod.2025.11.002_b0920","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1089\/ten.2006.0302","article-title":"Facilitated endogenous repair: making tissue engineering simple, practical, and economical","volume":"13","author":"Evans","year":"2007","journal-title":"Tissue Eng."},{"key":"10.1016\/j.mattod.2025.11.002_b0925","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/S0736-0266(02)00108-0","article-title":"Engineered allogeneic mesenchymal stem cells repair femoral segmental defect in rats","volume":"21","author":"Tsuchida","year":"2003","journal-title":"J. Orthop. Res."},{"key":"10.1016\/j.mattod.2025.11.002_b0930","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1002\/jcb.22440","article-title":"Derivation of murine induced pluripotent stem cells (iPS) and assessment of their differentiation toward osteogenic lineage","volume":"109","author":"Li","year":"2010","journal-title":"J. Cell. Biochem."},{"key":"10.1016\/j.mattod.2025.11.002_b0935","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1089\/ten.2005.11.120","article-title":"Healing of critically sized femoral defects, using genetically modified mesenchymal stem cells from human adipose tissue","volume":"11","author":"Peterson","year":"2005","journal-title":"Tissue Eng."},{"key":"10.1016\/j.mattod.2025.11.002_b0940","unstructured":"B.E. R., Transcript-Activated Matrix: Scaffold-Based Delivery of mRNA for Enhanced Bone Repair, Orthopaedic Proceedings, 100-B (2018) 71-71. https:\/\/doi.org\/10.1302\/1358-992x.2018.16.071."},{"key":"10.1016\/j.mattod.2025.11.002_b0945","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1038\/10473","article-title":"Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration","volume":"5","author":"Bonadio","year":"1999","journal-title":"Nat. Med."},{"key":"10.1016\/j.mattod.2025.11.002_b0950","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1002\/jbm.a.20036","article-title":"Fabrication and in vitro testing of polymeric delivery system for condensed DNA","volume":"67","author":"Huang","year":"2003","journal-title":"J. Biomed. Mater. Res. A"},{"key":"10.1016\/j.mattod.2025.11.002_b0955","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.biomaterials.2016.07.017","article-title":"Non-viral gene activated matrices for mesenchymal stem cells based tissue engineering of bone and cartilage","volume":"104","author":"Raisin","year":"2016","journal-title":"Biomaterials"},{"key":"10.1016\/j.mattod.2025.11.002_b0960","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1208\/s12248-016-9982-2","article-title":"Bone regeneration using gene-activated matrices","volume":"19","author":"D'Mello","year":"2017","journal-title":"AAPS J."},{"key":"10.1016\/j.mattod.2025.11.002_b0965","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1089\/107632701753213183","article-title":"Effect of pore size and void fraction on cellular adhesion, proliferation, and matrix deposition","volume":"7","author":"Zeltinger","year":"2001","journal-title":"Tissue Eng."},{"key":"10.1016\/j.mattod.2025.11.002_b0970","first-page":"768","article-title":"RAGE and its ligands in bone metabolism","volume":"3","author":"Zhou","year":"2011","journal-title":"Front. Biosci. (Schol. Ed.)"},{"key":"10.1016\/j.mattod.2025.11.002_b0975","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1586\/17434440.2015.1059274","article-title":"Additive manufacturing in biomedical sciences and the need for definitions and norms","volume":"12","author":"Chhaya","year":"2015","journal-title":"Expert Rev. Med. Devices"},{"key":"10.1016\/j.mattod.2025.11.002_b0980","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/S1369-7021(11)70058-X","article-title":"Biomaterials & scaffolds for tissue engineering","volume":"14","author":"O'brien","year":"2011","journal-title":"Mater. Today"},{"key":"10.1016\/j.mattod.2025.11.002_b0985","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0168-3659(02)00369-3","article-title":"Controllable delivery of non-viral DNA from porous scaffolds","volume":"86","author":"Jang","year":"2003","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mattod.2025.11.002_b0990","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.addr.2007.03.017","article-title":"Matrices and scaffolds for DNA delivery in tissue engineering","volume":"59","author":"De Laporte","year":"2007","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.mattod.2025.11.002_b0995","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1080\/09205063.1991.9756659","article-title":"Why proteins prefer interfaces","volume":"2","author":"Norde","year":"1991","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"10.1016\/j.mattod.2025.11.002_b1000","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1002\/polb.22259","article-title":"Biomedical applications of Biodegradable Polymers","volume":"49","author":"Ulery","year":"2011","journal-title":"J. Polym. Sci. B Polym. Phys."},{"key":"10.1016\/j.mattod.2025.11.002_b1005","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.3390\/nano10112142","article-title":"A comprehensive review of the covalent immobilization of biomolecules onto electrospun nanofibers","volume":"10","author":"Smith","year":"2020","journal-title":"Nanomaterials"},{"key":"10.1016\/j.mattod.2025.11.002_b1010","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1002\/jor.24321","article-title":"Scaffold-based delivery of nucleic acid therapeutics for enhanced bone and cartilage repair","volume":"37","author":"Kelly","year":"2019","journal-title":"J. Orthop. Res."},{"key":"10.1016\/j.mattod.2025.11.002_b1015","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1089\/scd.2016.0171","article-title":"Modified mRNA for BMP-2 in combination with biomaterials serves as a transcript-activated matrix for effectively inducing osteogenic pathways in stem cells","volume":"26","author":"Balmayor","year":"2017","journal-title":"Stem Cells Dev."},{"key":"10.1016\/j.mattod.2025.11.002_b1020","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1089\/ten.tea.2017.0444","article-title":"Messenger RNA delivery for tissue engineering and regenerative medicine applications","volume":"25","author":"Patel","year":"2019","journal-title":"Tissue Eng. Part A"},{"key":"10.1016\/j.mattod.2025.11.002_b1025","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/adma.201303233","article-title":"25th anniversary article: rational design and applications of hydrogels in regenerative medicine","volume":"26","author":"Annabi","year":"2014","journal-title":"Adv. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b1030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mser.2017.07.001","article-title":"Spatially and temporally controlled hydrogels for tissue engineering","volume":"119","author":"Leijten","year":"2017","journal-title":"Mater. Sci. Eng. R. Rep."},{"key":"10.1016\/j.mattod.2025.11.002_b1035","doi-asserted-by":"crossref","first-page":"4487","DOI":"10.1016\/j.actbio.2012.08.012","article-title":"Functionalized, biodegradable hydrogels for control over sustained and localized siRNA delivery to incorporated and surrounding cells","volume":"9","author":"Nguyen","year":"2013","journal-title":"Acta Biomater."},{"key":"10.1016\/j.mattod.2025.11.002_b1040","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.201601041","article-title":"Engineered hydrogels for local and sustained delivery of RNA-interference therapies","volume":"6","author":"Wang","year":"2017","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b1045","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1586\/14760584.2015.973859","article-title":"mRNA transcript therapy","volume":"14","author":"Weissman","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.mattod.2025.11.002_b1050","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/sj.gt.3302212","article-title":"Orthopaedics: gene therapy's dark horse","volume":"11","author":"Evans","year":"2004","journal-title":"Gene Ther."},{"key":"10.1016\/j.mattod.2025.11.002_b1055","doi-asserted-by":"crossref","first-page":"15022","DOI":"10.1038\/s41598-025-99979-6","article-title":"BMP-2 mRNA-transfected BMSCs promote superior calvarial bone regeneration","volume":"15","author":"Surisaeng","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mattod.2025.11.002_b1060","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.spinee.2017.07.171","article-title":"BMP-2\/7 heterodimer strongly induces bone regeneration in the absence of increased soft tissue inflammation","volume":"18","author":"Kaito","year":"2018","journal-title":"Spine J."},{"key":"10.1016\/j.mattod.2025.11.002_b1065","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1021\/acs.molpharmaceut.0c01042","article-title":"Transcript-Activated Coatings on Titanium Mediate Cellular Osteogenesis for Enhanced Osteointegration","volume":"18","author":"Fayed","year":"2021","journal-title":"Mol. Pharm."},{"key":"10.1016\/j.mattod.2025.11.002_b1070","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202405047","article-title":"D'Este, Implementing BMP\u20107 Chemically Modified RNA for Bone Regeneration with 3D Printable Hyaluronic Acid\u2010Collagen Granular Gels","author":"van der Heide","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"10.1016\/j.mattod.2025.11.002_b1075","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1002\/jcp.23031","article-title":"Orthopedic gene therapy\u2013lost in translation?","volume":"227","author":"Evans","year":"2012","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.mattod.2025.11.002_b1080","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1302\/2046-3758.810.BJR-2019-0265","article-title":"The vicissitudes of gene therapy","volume":"8","author":"Evans","year":"2019","journal-title":"Bone Joint Res"},{"key":"10.1016\/j.mattod.2025.11.002_b1085","first-page":"683","article-title":"Focus: Genome Editing: Regulatory Considerations for Gene Therapy Products in the US","volume":"90","author":"Halioua-Haubold","year":"2017","journal-title":"EU, and Japan, the Yale Journal of Biology and Medicine"},{"key":"10.1016\/j.mattod.2025.11.002_b1090","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1007\/s00198-004-1627-0","article-title":"An estimate of the worldwide prevalence, mortality and disability associated with hip fracture","volume":"15","author":"Johnell","year":"2004","journal-title":"Osteoporos Int."},{"key":"10.1016\/j.mattod.2025.11.002_b1095","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1089\/ten.teb.2011.0475","article-title":"Bone tissue engineering and regeneration: from discovery to the clinic\u2013an overview","volume":"17","author":"O'Keefe","year":"2011","journal-title":"Tissue Eng. Part B Rev."},{"key":"10.1016\/j.mattod.2025.11.002_b1100","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1177\/2051013613508729","article-title":"A development that may evolve into a revolution in medicine: mRNA as the basis for novel, nucleotide-based vaccines and drugs","volume":"2","author":"Kallen","year":"2014","journal-title":"Ther Adv Vaccines"},{"key":"10.1016\/j.mattod.2025.11.002_b1105","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1177\/01410768211053006","article-title":"The costs of coronavirus vaccines and their pricing","volume":"114","author":"Light","year":"2021","journal-title":"J. R. Soc. Med."},{"key":"10.1016\/j.mattod.2025.11.002_b1110","article-title":"Covid-19: countries are learning what others paid for vaccines","volume":"372","author":"Dyer","year":"2021","journal-title":"BMJ: British Medical Journal (online)"},{"key":"10.1016\/j.mattod.2025.11.002_b1115","doi-asserted-by":"crossref","first-page":"3","DOI":"10.3390\/vaccines9010003","article-title":"Resources, production scales and time required for producing RNA vaccines for the global pandemic demand","volume":"9","author":"Kis","year":"2020","journal-title":"Vaccines"},{"key":"10.1016\/j.mattod.2025.11.002_b1120","first-page":"e41","article-title":"Safety and adverse events Related to COVID-19 mRNA Vaccines; a Systematic Review","volume":"10","author":"SeyedAlinaghi","year":"2022","journal-title":"Arch Acad Emerg Med"},{"key":"10.1016\/j.mattod.2025.11.002_b1125","doi-asserted-by":"crossref","first-page":"3326","DOI":"10.1016\/j.vaccine.2019.04.074","article-title":"Ciaramella, mRNA vaccines against H10N8 and H7N9 influenza viruses of pandemic potential are immunogenic and well tolerated in healthy adults in phase 1 randomized clinical trials","volume":"37","author":"Feldman","year":"2019","journal-title":"Vaccine"},{"key":"10.1016\/j.mattod.2025.11.002_b1130","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1016\/S0140-6736(17)31665-3","article-title":"Safety and immunogenicity of a mRNA rabies vaccine in healthy adults: an open-label, non-randomised, prospective, first-in-human phase 1 clinical trial","volume":"390","author":"Alberer","year":"2017","journal-title":"Lancet"},{"key":"10.1016\/j.mattod.2025.11.002_b1135","doi-asserted-by":"crossref","first-page":"12542","DOI":"10.1074\/jbc.M310175200","article-title":"mRNA is an endogenous ligand for Toll-like receptor 3","volume":"279","author":"Karik\u00f3","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mattod.2025.11.002_b1140","doi-asserted-by":"crossref","unstructured":"M.K. Mekhemar, C.E. D\u00f6rfer, K.M.F. El-Sayed, Toll-like receptors: The key of immunotherapy in MSCs, Immunoregulatory aspects of immunotherapy, IntechOpen2018. https:\/\/doi.org\/10.5772\/intechopen.76644.","DOI":"10.5772\/intechopen.76644"},{"key":"10.1016\/j.mattod.2025.11.002_b1145","first-page":"71","article-title":"Measuring ER stress and the unfolded protein response using mammalian tissue culture system, Methods in enzymology","author":"Oslowski","year":"2011","journal-title":"Elsevier"},{"key":"10.1016\/j.mattod.2025.11.002_b1150","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.jconrel.2022.03.046","article-title":"The replacement of helper lipids with charged alternatives in lipid nanoparticles facilitates targeted mRNA delivery to the spleen and lungs","volume":"345","author":"LoPresti","year":"2022","journal-title":"J. Control. Release"}],"container-title":["Materials Today"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1369702125004602?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1369702125004602?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:36:36Z","timestamp":1781015796000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1369702125004602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":230,"alternative-id":["S1369702125004602"],"URL":"https:\/\/doi.org\/10.1016\/j.mattod.2025.11.002","relation":{},"ISSN":["1369-7021"],"issn-type":[{"value":"1369-7021","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Engineering bone tissue with mRNA: from molecular design and delivery to clinical applications","name":"articletitle","label":"Article Title"},{"value":"Materials Today","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mattod.2025.11.002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}