{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T06:05:24Z","timestamp":1776233124303,"version":"3.50.1"},"reference-count":24,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100005363","name":"Universidad de Buenos Aires","doi-asserted-by":"crossref","award":["20020100100594\/2011"],"award-info":[{"award-number":["20020100100594\/2011"]}],"id":[{"id":"10.13039\/501100005363","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002923","name":"CONICET","doi-asserted-by":"crossref","award":["PIP 2012\/112-201101-00091"],"award-info":[{"award-number":["PIP 2012\/112-201101-00091"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ANPCyT-FONCyT","award":["PICT 2010\/05-1130"],"award-info":[{"award-number":["PICT 2010\/05-1130"]}]},{"name":"ANPCyT-FONCyT","award":["PICT 2012\/05-1090"],"award-info":[{"award-number":["PICT 2012\/05-1090"]}]},{"name":"FIS","award":["PI10\/00219"],"award-info":[{"award-number":["PI10\/00219"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Endocrinology"],"published-print":{"date-parts":[[2015,3]]},"DOI":"10.1016\/j.mce.2014.12.026","type":"journal-article","created":{"date-parts":[[2015,1,7]],"date-time":"2015-01-07T12:04:55Z","timestamp":1420632295000},"page":"9-15","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Kinetic characterization of human thyroperoxidase. Normal and pathological enzyme expression in Baculovirus System: A molecular model of functional expression"],"prefix":"10.1016","volume":"404","author":[{"given":"Fiorella S.","family":"Belforte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra M.","family":"Targovnik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo M.","family":"Gonz\u00e1lez-Lebrero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Osorio Larroche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3547-5458","authenticated-orcid":false,"given":"Cintia E.","family":"Citterio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rogelio","family":"Gonz\u00e1lez-Sarmiento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda V.","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor M.","family":"Targovnik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina M.","family":"Rivolta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mce.2014.12.026_bib0010","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/A:1026488408951","article-title":"Insect cells as hosts for the expression of recombinant glycoproteins","volume":"16","author":"Altmann","year":"1999","journal-title":"Glycoconj. J."},{"key":"10.1016\/j.mce.2014.12.026_bib0015","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/j.1365-2265.2011.04249.x","article-title":"Congenital goitrous hypothyroidism: mutation analysis in the thyroid peroxidase gene","volume":"76","author":"Belforte","year":"2012","journal-title":"Clin. Endocrinol"},{"key":"10.1016\/j.mce.2014.12.026_bib0020","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem"},{"key":"10.1016\/j.mce.2014.12.026_bib0025","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.vaccine.2012.01.016","article-title":"Recombinant protein vaccines produced in insect cells","volume":"30","author":"Cox","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.mce.2014.12.026_bib0030","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.biotechadv.2009.01.008","article-title":"Production of recombinant proteins by microbes and higher organisms","volume":"27","author":"Demain","year":"2009","journal-title":"Biotechnol. Adv"},{"key":"10.1016\/j.mce.2014.12.026_bib0035","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1006\/jaut.1996.0071","article-title":"Purification and characterization of a recombinant human thyroid peroxidase expressed in insect cells","volume":"9","author":"Fan","year":"1996","journal-title":"J. Autoimmun"},{"key":"10.1016\/j.mce.2014.12.026_bib0040","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0167-4838(99)00128-4","article-title":"Distinct immunological and biochemical properties of thyroid peroxidase purified from human thyroid glands and recombinant protein produced in insect cells","volume":"1433","author":"Gardas","year":"1999","journal-title":"Biochim. Biophys. Acta"},{"issue":"15","key":"10.1016\/j.mce.2014.12.026_bib0045","first-page":"18","article-title":"Congenital hypothyroidism","volume":"21","author":"Gruters","year":"1992","journal-title":"Pediatr. Ann"},{"key":"10.1016\/j.mce.2014.12.026_bib0050","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1006\/cbir.1996.0052","article-title":"Protein retention in the endoplasmic reticulum of insect cells is not compromised by baculovirus infection","volume":"20","author":"Henderson","year":"1996","journal-title":"Cell Biol. Int"},{"key":"10.1016\/j.mce.2014.12.026_bib0055","doi-asserted-by":"crossref","first-page":"R13","DOI":"10.1677\/joe.0.160R013","article-title":"Recombinant human thyroid peroxidase expressed in insect cells is soluble at high concentrations and forms diffracting crystals","volume":"160","author":"Hendry","year":"1999","journal-title":"J. Endocrinol"},{"key":"10.1016\/j.mce.2014.12.026_bib0060","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/192659a0","article-title":"Demonstration and some properties of peroxidase in pig thyroid gland","volume":"192","author":"Hosoya","year":"1961","journal-title":"Nature"},{"key":"10.1016\/j.mce.2014.12.026_bib0065","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1756-0500-2-63","article-title":"96-Well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells","volume":"2","author":"K\u00e4rkk\u00e4inen","year":"2009","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.mce.2014.12.026_bib0070","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0303-7207(93)90124-3","article-title":"Expression of human thyroid peroxidase in insect cells using recombinant baculovirus","volume":"93","author":"Kendler","year":"1993","journal-title":"Mol. Cell. Endocrinol"},{"key":"10.1016\/j.mce.2014.12.026_bib0075","first-page":"810","article-title":"A method for producing recombinant baculovirus expression vectors at high frequency","volume":"14","author":"Kitts","year":"1993","journal-title":"Biotechniques"},{"key":"10.1016\/j.mce.2014.12.026_bib0080","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/nbt1095","article-title":"Baculovirus as versatile vectors for protein expression in insect and mammalian cells","volume":"23","author":"Kost","year":"2005","journal-title":"Nat. Biotechnol"},{"key":"10.1016\/j.mce.2014.12.026_bib0085","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1210\/jcem-72-2-471","article-title":"Genetic linkage studies of thyroid peroxidase (TPO) gene in families with TPO deficiency","volume":"72","author":"Mangklabruks","year":"1991","journal-title":"J. Clin. Endocrinol. Metab"},{"key":"10.1016\/j.mce.2014.12.026_bib0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2353\/jmoldx.2007.060081","article-title":"Standard mutation nomenclature in molecular diagnostics: practical and educational challenges","volume":"9","author":"Ogino","year":"2007","journal-title":"J. Mol. Diagn"},{"key":"10.1016\/j.mce.2014.12.026_bib0095","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1136\/jmg.2004.024158","article-title":"Genetics of congenital hypothyroidism","volume":"42","author":"Park","year":"2005","journal-title":"J. Med. Genet"},{"key":"10.1016\/j.mce.2014.12.026_bib0100","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1750-1172-5-17","article-title":"Congenital hypothyroidism","volume":"5","author":"Rastogi","year":"2010","journal-title":"Orphanet J. Rare Dis"},{"key":"10.1016\/j.mce.2014.12.026_bib0105","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1002\/humu.9175","article-title":"Five novel inactivating mutations in the thyroid peroxidase gene responsible for congenital goiter and iodide organification defect","volume":"22","author":"Rivolta","year":"2003","journal-title":"Hum. Mutat"},{"key":"10.1016\/j.mce.2014.12.026_bib0110","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1365-2265.2007.02869.x","article-title":"Two compound heterozygous mutations (c.215delA\/c.2422T\u2192C and c.387delC\/c.1159G\u2192A) in the thyroid peroxidase gene responsible for congenital goitre and iodide organification defect","volume":"67","author":"Rivolta","year":"2007","journal-title":"Clin. Endocrinol"},{"key":"10.1016\/j.mce.2014.12.026_bib0115","doi-asserted-by":"crossref","first-page":"S94","DOI":"10.1016\/j.jip.2011.05.009","article-title":"Quality control and analytical methods for baculovirus-based products","volume":"107","author":"Rold\u00e3o","year":"2011","journal-title":"J. Invertebr. Pathol"},{"key":"10.1016\/j.mce.2014.12.026_bib0120","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.procbio.2010.02.003","article-title":"Horseradish peroxidase production from Spodoptera frugiperda larvae: a simple and inexpensive method","volume":"45","author":"Targovnik","year":"2010","journal-title":"Proc. Biochem"},{"key":"10.1016\/j.mce.2014.12.026_bib0125","series-title":"Practice and Theory of Enzyme Immunoassays","author":"Tjissen","year":"1985"}],"container-title":["Molecular and Cellular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720715000040?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720715000040?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T08:56:04Z","timestamp":1537952164000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0303720715000040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3]]},"references-count":24,"alternative-id":["S0303720715000040"],"URL":"https:\/\/doi.org\/10.1016\/j.mce.2014.12.026","relation":{},"ISSN":["0303-7207"],"issn-type":[{"value":"0303-7207","type":"print"}],"subject":[],"published":{"date-parts":[[2015,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Kinetic characterization of human thyroperoxidase. Normal and pathological enzyme expression in Baculovirus System: A molecular model of functional expression","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Endocrinology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mce.2014.12.026","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ireland Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}