{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:36:46Z","timestamp":1791099406486,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"King's Health Partner's Research"},{"name":"Development Challenge Fund"},{"DOI":"10.13039\/501100000380","name":"Guy's and St Thomas' Charity","doi-asserted-by":"publisher","award":["R121151"],"award-info":[{"award-number":["R121151"]}],"id":[{"id":"10.13039\/501100000380","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research (NIHR)","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000764","name":"Kings College London","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000764","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010872","name":"King's College Hospital NHS Foundation Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010872","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Endocrinology"],"published-print":{"date-parts":[[2018,12]]},"DOI":"10.1016\/j.mce.2018.06.011","type":"journal-article","created":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T08:40:28Z","timestamp":1529397628000},"page":"140-147","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"special_numbering":"C","title":["Site-specific differences in osteoblast phenotype, mechanical loading response and estrogen receptor-related gene expression"],"prefix":"10.1016","volume":"477","author":[{"given":"Borzo","family":"Gharibi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandeep S.","family":"Ghuman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C.F.","family":"Rawlinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agamemnon E.","family":"Grigoriadis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis J.","family":"Hughes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mce.2018.06.011_bib1","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.bone.2005.10.027","article-title":"Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals","volume":"38","author":"Akintoye","year":"2006","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib2","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1172\/JCI65910","article-title":"Estrogen receptor-\u03b1 signaling in osteoblast progenitors stimulates cortical bone accrual","volume":"123","author":"Almeida","year":"2013","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.mce.2018.06.011_bib3","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1152\/physrev.00033.2015","article-title":"Estrogens and androgens in skeletal physiology and pathophysiology","volume":"97","author":"Almeida","year":"2017","journal-title":"Physiol. Rev."},{"key":"10.1016\/j.mce.2018.06.011_bib4","doi-asserted-by":"crossref","first-page":"32005","DOI":"10.1074\/jbc.M304698200","article-title":"Age-related changes in the biomolecular mechanisms of calvarial osteoblast biology affect fibroblast growth factor-2 signaling and osteogenesis","volume":"278","author":"Cowan","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mce.2018.06.011_bib5","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/S0092-8674(00)80257-3","article-title":"Osf2\/Cbfa1: a transcriptional activator of osteoblast differentiation","volume":"89","author":"Ducy","year":"1997","journal-title":"Cell"},{"key":"10.1016\/j.mce.2018.06.011_bib6","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1096\/fasebj.13.10.1219","article-title":"Functional heterogeneity of osteoclasts: matrix metalloproteinases participate in osteoclastic resorption of calvarial bone but not in resorption of long bone","volume":"13","author":"Everts","year":"1999","journal-title":"Faseb. J."},{"key":"10.1016\/j.mce.2018.06.011_bib7","doi-asserted-by":"crossref","first-page":"42259","DOI":"10.1074\/jbc.M105888200","article-title":"RERG is a novel ras-related, estrogen-regulated and growth-inhibitory gene in breast cancer","volume":"276","author":"Finlin","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mce.2018.06.011_bib8","doi-asserted-by":"crossref","first-page":"9035","DOI":"10.1074\/jbc.M112.405456","article-title":"Estrogen receptor \u03b1 mediates proliferation of osteoblastic cells stimulated by estrogen and mechanical strain, but their acute down-regulation of the Wnt antagonist Sost is mediated by estrogen receptor \u03b2","volume":"288","author":"Galea","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mce.2018.06.011_bib9","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nmat2505","article-title":"Comparative materials differences revealed in engineered bone as a function of cell-specific differentiation","volume":"8","author":"Gentleman","year":"2009","journal-title":"Nat. Mater."},{"key":"10.1016\/j.mce.2018.06.011_bib10","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nmat2505","article-title":"Comparative materials differences revealed in engineered bone as a function of cell-specific differentiation","volume":"8","author":"Gentleman","year":"2009","journal-title":"Nat. Mater."},{"key":"10.1016\/j.mce.2018.06.011_bib11","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1089\/ten.tea.2012.0623","article-title":"Gene expression responses to mechanical stimulation of mesenchymal stem cells seeded on calcium phosphate cement","volume":"19","author":"Gharibi","year":"2013","journal-title":"Tissue Eng."},{"key":"10.1016\/j.mce.2018.06.011_bib12","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.archoralbio.2016.06.026","article-title":"Heterogeneity of osteoclast activity and bone turnover in different skeletal sites","volume":"71","author":"Goldberg","year":"2016","journal-title":"Arch. Oral Biol."},{"key":"10.1016\/j.mce.2018.06.011_bib13","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1016\/S8756-3282(97)84305-0","article-title":"O4. Comparison of physiological strains in the human skull and tibia","volume":"19","author":"Hillam","year":"1996","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib14","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1016\/j.bbrc.2011.12.031","article-title":"Structural differences in the osteocyte network between the calvaria and long bone revealed by three-dimensional fluorescence morphometry, possibly reflecting distinct mechano-adaptations and sensitivities","volume":"417","author":"Himeno-Ando","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mce.2018.06.011_bib15","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1038\/nature02079","article-title":"Bone recognition mechanism of porcine osteocalcin from crystal structure","volume":"425","author":"Hoang","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.mce.2018.06.011_bib16","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/(SICI)1097-0177(199709)210:1<33::AID-AJA4>3.0.CO;2-2","article-title":"Mouse clavicular development: analysis of wild-type and cleidocranial dysplasia mutant mice","volume":"210","author":"Huang","year":"1997","journal-title":"Dev. Dynam."},{"key":"10.1016\/j.mce.2018.06.011_bib17","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1016\/0006-291X(91)90971-9","article-title":"Effect of 17 \u03b2 -estradiol on the proliferation of osteoblastic MC3T3-E1 cells via human monocytes","volume":"178","author":"Kanatani","year":"1991","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.mce.2018.06.011_bib18","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/S8756-3282(98)00045-3","article-title":"Bone responses at various skeletal sites to human parathyroid hormone in ovariectomized rats: effects of long-term administration, withdrawal, and readministration","volume":"22","author":"Kishi","year":"1998","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib19","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1128\/MCB.01550-06","article-title":"Induction of osteoblast differentiation by selective activation of kinase-mediated actions of the estrogen receptor","volume":"27","author":"Kousteni","year":"2007","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.mce.2018.06.011_bib20","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1242\/dev.023788","article-title":"Embryonic origin and Hox status determine progenitor cell fate during adult bone regeneration","volume":"135","author":"Leucht","year":"2008","journal-title":"Development"},{"key":"10.1016\/j.mce.2018.06.011_bib21","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/jbmr.193","article-title":"Gene expression patterns in bone following mechanical loading","volume":"26","author":"MantilaRoosa","year":"2011","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.mce.2018.06.011_bib22","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1006\/dbio.2001.0330","article-title":"A population of caudally migrating cranial neural crest cells: functional and evolutionary implications","volume":"236","author":"McGonnell","year":"2001","journal-title":"Dev. Biol."},{"key":"10.1016\/j.mce.2018.06.011_bib23","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1677\/joe.0.1760349","article-title":"Mutual antagonism of estrogen receptors alpha and beta and their preferred interactions with steroid receptor coactivators in human osteoblastic cell lines","volume":"176","author":"Monroe","year":"2003","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.mce.2018.06.011_bib24","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1080\/03008200902846262","article-title":"Age and skeletal sites affect BMP-2 responsiveness of human bone marrow stromal cells","volume":"50","author":"Osyczka","year":"2009","journal-title":"Connect. Tissue Res."},{"key":"10.1016\/j.mce.2018.06.011_bib25","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s00223-005-0289-z","article-title":"Calvarial osteoclasts express a higher level of tartrate-resistant acid phosphatase than long bone osteoclasts and activation does not depend on cathepsin K or L activity","volume":"79","author":"Perez-Amodio","year":"2006","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.mce.2018.06.011_bib26","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1101\/gad.1449506","article-title":"Genetic regulation of bone mass and susceptibility to osteoporosis","volume":"20","author":"Ralston","year":"2006","journal-title":"Genes Dev."},{"key":"10.1016\/j.mce.2018.06.011_bib27","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1016\/j.jbiomech.2007.11.024","article-title":"Compressive forces induce osteogenic gene expression in calvarial osteoblasts","volume":"41","author":"Rath","year":"2008","journal-title":"J. Biomech."},{"key":"10.1016\/j.mce.2018.06.011_bib28","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1002\/jbmr.5650100813","article-title":"Calvarial and limb bone cells in organ and monolayer culture do not show the same early responses to dynamic mechanical strain","volume":"10","author":"Rawlinson","year":"1995","journal-title":"J. Bone Miner. Res."},{"key":"10.1016\/j.mce.2018.06.011_bib29","doi-asserted-by":"crossref","first-page":"e8358","DOI":"10.1371\/journal.pone.0008358","article-title":"Adult rat bones maintain distinct regionalized expression of markers associated with their development","volume":"4","author":"Rawlinson","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.mce.2018.06.011_bib30","doi-asserted-by":"crossref","first-page":"e119","DOI":"10.1371\/journal.pgen.0020119","article-title":"Anatomic demarcation by positional variation in fibroblast gene expression programs","volume":"2","author":"Rinn","year":"2006","journal-title":"PLoS Genet."},{"key":"10.1016\/j.mce.2018.06.011_bib31","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1101\/gad.1610508","article-title":"A dermal HOX transcriptional program regulates site-specific epidermal fate","volume":"22","author":"Rinn","year":"2008","journal-title":"Genes Dev."},{"key":"10.1016\/j.mce.2018.06.011_bib32","first-page":"565","article-title":"Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1alpha gene expression in primary culture of rat calvarial osteoblast-like cells","volume":"18","author":"Rucinski","year":"2006","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.mce.2018.06.011_bib33","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1007\/BF02556258","article-title":"The matrix of endochondral bone differs from the matrix of intramembranous bone","volume":"49","author":"Scott","year":"1991","journal-title":"Calcif. Tissue Int."},{"key":"10.1016\/j.mce.2018.06.011_bib34","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S8756-3282(99)00251-3","article-title":"Relationships between bone protein and mineral in developing porcine long bone and calvaria","volume":"26","author":"Sodek","year":"2000","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib35","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1677\/joe.0.1560051","article-title":"Differential effects of systemic prostaglandin E2 on bone mass in rat long bones and calvariae","volume":"156","author":"Suponitzky","year":"1998","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.mce.2018.06.011_bib36","unstructured":"Timoshenko, S. P. & Goodier, J. N. in 166\u2013167 (McGraw-Hill, Singapore, 1982)."},{"key":"10.1016\/j.mce.2018.06.011_bib37","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.bone.2008.05.009","article-title":"Differences in matrix composition between calvaria and long bone in mice suggest differences in biomechanical properties and resorption: special emphasis on collagen","volume":"43","author":"van den Bos","year":"2008","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib38","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.bone.2016.02.010","article-title":"Osteoblasts of calvaria induce higher numbers of osteoclasts than osteoblasts from long bone","volume":"86","author":"Wan","year":"2016","journal-title":"Bone"},{"key":"10.1016\/j.mce.2018.06.011_bib39","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jclinepi.2010.07.007","article-title":"Which fractures are most attributable to osteoporosis?","volume":"64","author":"Warriner","year":"2011","journal-title":"J. Clin. Epidemiol."},{"key":"10.1016\/j.mce.2018.06.011_bib40","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.bbrc.2010.03.063","article-title":"Long bone osteoclasts display an augmented osteoclast phenotype compared to calvarial osteoclasts","volume":"394","author":"Zenger","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Molecular and Cellular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720718301977?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720718301977?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,20]],"date-time":"2018-09-20T23:27:51Z","timestamp":1537486071000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0303720718301977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12]]},"references-count":40,"alternative-id":["S0303720718301977"],"URL":"https:\/\/doi.org\/10.1016\/j.mce.2018.06.011","relation":{},"ISSN":["0303-7207"],"issn-type":[{"value":"0303-7207","type":"print"}],"subject":[],"published":{"date-parts":[[2018,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Site-specific differences in osteoblast phenotype, mechanical loading response and estrogen receptor-related gene expression","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Endocrinology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mce.2018.06.011","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}