{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:59:03Z","timestamp":1788778743959,"version":"build-2803163510"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN204319"],"award-info":[{"award-number":["IN204319"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN209520"],"award-info":[{"award-number":["IN209520"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005739","name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["EPE 2018\\u20132019"],"award-info":[{"award-number":["EPE 2018\\u20132019"]}],"id":[{"id":"10.13039\/501100005739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["137725"],"award-info":[{"award-number":["137725"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["CVU 826027"],"award-info":[{"award-number":["CVU 826027"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["2018-000068-02NACF-17950"],"award-info":[{"award-number":["2018-000068-02NACF-17950"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Endocrinology"],"published-print":{"date-parts":[[2020,6]]},"DOI":"10.1016\/j.mce.2020.110801","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T21:32:08Z","timestamp":1586381528000},"page":"110801","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Testosterone augments \u03b22 adrenergic receptor genomic transcription increasing salbutamol relaxation in airway smooth muscle"],"prefix":"10.1016","volume":"510","author":[{"given":"Abril","family":"Carbajal-Garc\u00eda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Reyes-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda F.","family":"Casas-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Flores-Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"D\u00edaz-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor","family":"Sol\u00eds-Chagoy\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina","family":"Sommer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"Monta\u00f1o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mce.2020.110801_bib1","doi-asserted-by":"crossref","first-page":"13239","DOI":"10.1074\/jbc.271.22.13239","article-title":"Expression and function of voltage-dependent potassium channel genes in human airway smooth muscle","volume":"271","author":"Adda","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mce.2020.110801_bib2","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1210\/en.2014-1198","article-title":"Identification and characterization of membrane androgen receptors in the ZIP9 zinc transporter subfamily: I. Discovery in female atlantic croaker and evidence ZIP9 mediates testosterone-induced apoptosis of ovarian follicle cells","volume":"155","author":"Berg","year":"2014","journal-title":"Endocrinology"},{"key":"10.1016\/j.mce.2020.110801_bib3","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/bph.12905","article-title":"The role of adenylyl cyclase isoform 6 in \u03b2-adrenoceptor signalling in murine airways","volume":"172","author":"Birrell","year":"2015","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib4","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.ejphar.2008.10.039","article-title":"Modulatory role of endogenous androgens on airway smooth muscle tone in isolated Guinea-pig and bovine trachea; involvement of \u03b22-adrenoceptors, the polyamine system and external calcium","volume":"601","author":"Bordallo","year":"2008","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib5","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1113\/jphysiol.1992.sp019005","article-title":"Delayed rectifier potassium channels in canine and porcine airway smooth muscle cells","volume":"447","author":"Boyle","year":"1992","journal-title":"J. Physiol."},{"key":"10.1016\/j.mce.2020.110801_bib6","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19082223","article-title":"Mechanisms of PKA-dependent potentiation of Kv7.5 channel activity in human airway smooth muscle cells","volume":"19","author":"Brueggemann","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mce.2020.110801_bib7","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.phrs.2006.09.006","article-title":"\u03b1-methyl-5-HT, a 5-HT2 receptor agonist, stimulates \u03b22-adrenoceptors in Guinea pig airway smooth muscle","volume":"54","author":"Campos-Bedolla","year":"2006","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.mce.2020.110801_bib8","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.lfs.2008.07.006","article-title":"Airway smooth muscle relaxation induced by 5-HT2A receptors: role of Na+\/K+-ATPase pump and Ca2+-activated K+ channels","volume":"83","author":"Campos-Bedolla","year":"2008","journal-title":"Life Sci."},{"key":"10.1016\/j.mce.2020.110801_bib9","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.mehy.2011.01.006","article-title":"Do low testosterone levels contribute to the pathogenesis of asthma?","volume":"76","author":"Canguven","year":"2011","journal-title":"Med. Hypotheses"},{"key":"10.1016\/j.mce.2020.110801_bib10","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1111\/bph.12365","article-title":"The GPCR, class C, group 6, subtype A (GPRC6A) receptor: from cloning to physiological function","volume":"171","author":"Clemmensen","year":"2014","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib11","doi-asserted-by":"crossref","first-page":"L915","DOI":"10.1152\/ajplung.00253.2005","article-title":"Paradoxical effect of salbutamol in a model of acute organophosphates intoxication in Guinea pigs: role of substance P release","volume":"292","author":"Chavez","year":"2007","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.mce.2020.110801_bib12","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1164\/ajrccm.162.1.9907008","article-title":"Differences in incidence of reported asthma related to age in men and women. A retrospective analysis of the data of the European Respiratory Health Survey","volume":"162","author":"de Marco","year":"2000","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.mce.2020.110801_bib13","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jsbmb.2013.05.009","article-title":"Nongenomic bronchodilating action elicited by dehydroepiandrosterone (DHEA) in a Guinea pig asthma model","volume":"138","author":"Espinoza","year":"2013","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"10.1016\/j.mce.2020.110801_bib14","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1152\/ajplung.00416.2018","article-title":"Regulation of IL-17A and implications for TGF-\u03b21 co-modulation of airway smooth muscle remodeling in severe asthma","volume":"316","author":"Evasovic","year":"2019","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.mce.2020.110801_bib15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nri3786","article-title":"Type 2 inflammation in asthma--present in most, absent in many","volume":"15","author":"Fahy","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.mce.2020.110801_bib16","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1476-5381.2009.00052.x","article-title":"Calcium-activated potassium channels and endothelial dysfunction: therapeutic options?","volume":"156","author":"Feletou","year":"2009","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib17","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.mce.2016.10.004","article-title":"Sex steroids effects on Guinea pig airway smooth muscle tone and intracellular Ca2+ basal levels","volume":"439","author":"Flores-Soto","year":"2017","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib18","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1111\/j.1476-5381.1983.tb09409.x","article-title":"Effect of steroids on \u03b2-adrenoceptor-mediated relaxation of pig bronchus","volume":"78","author":"Foster","year":"1983","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib19","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s11882-017-0686-1","article-title":"Mechanisms driving gender differences in asthma","volume":"17","author":"Fuseini","year":"2017","journal-title":"Curr. Allergy Asthma Rep."},{"key":"10.1016\/j.mce.2020.110801_bib20","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.4049\/jimmunol.1800293","article-title":"Testosterone decreases house dust mite-induced type 2 and IL-17A-mediated airway inflammation","volume":"201","author":"Fuseini","year":"2018","journal-title":"J. Immunol."},{"key":"10.1016\/j.mce.2020.110801_bib21","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0014-2999(93)90817-2","article-title":"Inhibition of \u03b2-adrenoceptor agonist relaxation of airway smooth muscle by Ca2+-activated K+ channel blockers","volume":"235","author":"Huang","year":"1993","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib22","first-page":"67","article-title":"Schild's equation and the best estimate of pA2 value and dissociation constant of an antagonist","volume":"40","author":"Jankovic","year":"1999","journal-title":"Croat. Med. J."},{"key":"10.1016\/j.mce.2020.110801_bib23","doi-asserted-by":"crossref","first-page":"S146","DOI":"10.1164\/ajrccm.158.supplement_2.13tac110","article-title":"The \u03b2-adrenoceptor","volume":"158","author":"Johnson","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.mce.2020.110801_bib24","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/S0022-3565(25)22919-6","article-title":"Selective inhibition of relaxation of Guinea-pig trachea by charybdotoxin, a potent Ca++-activated K+ channel inhibitor","volume":"255","author":"Jones","year":"1990","journal-title":"J. Pharmacol. Exp. Therapeut."},{"key":"10.1016\/j.mce.2020.110801_bib25","doi-asserted-by":"crossref","first-page":"215","DOI":"10.33594\/000000131","article-title":"Androgen receptor-mediated regulation of intracellular calcium in human airway smooth muscle cells","volume":"53","author":"Kalidhindi","year":"2019","journal-title":"Cell. Physiol. Biochem."},{"key":"10.1016\/j.mce.2020.110801_bib26","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1111\/bph.14097","article-title":"4-Aminopyridine: a pan voltage-gated potassium channel inhibitor that enhances Kv 7.4 currents and inhibits noradrenaline-mediated contraction of rat mesenteric small arteries","volume":"175","author":"Khammy","year":"2018","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib27","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1038\/sj.bjp.0706936","article-title":"Non-genomic effect of testosterone on airway smooth muscle","volume":"149","author":"Kouloumenta","year":"2006","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib28","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1084\/jem.20161807","article-title":"Androgen signaling negatively controls group 2 innate lymphoid cells","volume":"214","author":"Laffont","year":"2017","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.mce.2020.110801_bib29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/ni.3049","article-title":"The immunology of asthma","volume":"16","author":"Lambrecht","year":"2015","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.mce.2020.110801_bib30","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.mce.2020.110801_bib31","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1097\/FJC.0000000000000555","article-title":"Androgens mediate \u03b2-adrenergic vasorelaxation impairment using adenylyl Cyclase","volume":"71","author":"Lopez-Canales","year":"2018","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib32","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1042\/CS20170090","article-title":"Genomic and non-genomic effects of androgens in the cardiovascular system: clinical implications","volume":"131","author":"Lucas-Herald","year":"2017","journal-title":"Clin. Sci."},{"key":"10.1016\/j.mce.2020.110801_bib33","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s00424-011-0997-3","article-title":"Characterization of P2Y receptors mediating ATP induced relaxation in Guinea pig airway smooth muscle: involvement of prostaglandins and K+ channels","volume":"462","author":"Monta\u00f1o","year":"2011","journal-title":"Pfl\u00fcgers Archiv"},{"key":"10.1016\/j.mce.2020.110801_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1530\/JOE-14-0074","article-title":"Androgens are bronchoactive drugs that act by relaxing airway smooth muscle and preventing bronchospasm","volume":"222","author":"Monta\u00f1o","year":"2014","journal-title":"J. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib35","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.mce.2017.12.010","article-title":"Testosterone induces hyporesponsiveness by interfering with IP3 receptors in Guinea pig airway smooth muscle","volume":"473","author":"Monta\u00f1o","year":"2018","journal-title":"Mol. Cell. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib36","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/1477-7827-9-57","article-title":"Second to fourth digit ratio (2D:4D) and concentrations of circulating sex hormones in adulthood","volume":"9","author":"Muller","year":"2011","journal-title":"Reprod. Biol. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib37","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s00424-014-1534-y","article-title":"Testosterone-induced relaxation involves L-type and store-operated Ca2+ channels blockade, and PGE2 in Guinea pig airway smooth muscle","volume":"467","author":"Perusqu\u00eda","year":"2015","journal-title":"Pfl\u00fcgers Archiv"},{"key":"10.1016\/j.mce.2020.110801_bib38","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1210\/me.2015-1161","article-title":"Structural and functional evidence for testosterone activation of GPRC6A in peripheral tissues","volume":"29","author":"Pi","year":"2015","journal-title":"Mol. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib39","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/0014-2999(72)90194-X","article-title":"Inhibition of noradrenaline uptake 2 in the isolated rat heart by steroids, clonidine and methoxylated phenylethylamines","volume":"20","author":"Salt","year":"1972","journal-title":"Eur. J. Pharmacol."},{"key":"10.1016\/j.mce.2020.110801_bib40","doi-asserted-by":"crossref","first-page":"24980","DOI":"10.1074\/jbc.272.40.24980","article-title":"Efficient mammalian protein synthesis requires an intact F-actin system","volume":"272","author":"Stapulionis","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mce.2020.110801_bib41","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.ygcen.2011.04.019","article-title":"Testosterone modulation of cardiac \u03b2-adrenergic signals in a rat model of heart failure","volume":"172","author":"Sun","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib42","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1540\/jsmr.39.205","article-title":"MaxiK channel mediates \u03b22-adrenoceptor-activated relaxation to isoprenaline through cAMP-dependent and -independent mechanisms in Guinea-pig tracheal smooth muscle","volume":"39","author":"Tanaka","year":"2003","journal-title":"J. Smooth Muscle Res."},{"key":"10.1016\/j.mce.2020.110801_bib43","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ygcen.2017.04.016","article-title":"ZIP9, a novel membrane androgen receptor and zinc transporter protein","volume":"257","author":"Thomas","year":"2018","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1016\/j.mce.2020.110801_bib44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1210\/er.2010-0031","article-title":"Sex differences and sex steroids in lung health and disease","volume":"33","author":"Townsend","year":"2012","journal-title":"Endocr. Rev."},{"key":"10.1016\/j.mce.2020.110801_bib45","first-page":"L100","article-title":"Activation of KCa channels in airway smooth muscle cells by endogenous protein kinase A","volume":"271","author":"Wang","year":"1996","journal-title":"Am. J. Physiol."},{"key":"10.1016\/j.mce.2020.110801_bib46","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/j.anai.2018.01.016","article-title":"Hormones, sex, and asthma","volume":"120","author":"Yung","year":"2018","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"10.1016\/j.mce.2020.110801_bib47","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0306-9877(97)90020-7","article-title":"Does puberty interfere with asthma?","volume":"48","author":"Zannolli","year":"1997","journal-title":"Med. Hypotheses"},{"key":"10.1016\/j.mce.2020.110801_bib48","article-title":"Sexual dimorphism in the regulation of estrogen, progesterone, and androgen receptors by sex steroids in the rat airway smooth muscle cells","volume":"2016","author":"Zarazua","year":"2016","journal-title":"Internet J. Endocrinol."}],"container-title":["Molecular and Cellular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720720301015?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0303720720301015?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T14:14:51Z","timestamp":1778508891000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0303720720301015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":48,"alternative-id":["S0303720720301015"],"URL":"https:\/\/doi.org\/10.1016\/j.mce.2020.110801","relation":{},"ISSN":["0303-7207"],"issn-type":[{"value":"0303-7207","type":"print"}],"subject":[],"published":{"date-parts":[[2020,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Testosterone augments \u03b22 adrenergic receptor genomic transcription increasing salbutamol relaxation in airway smooth muscle","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Endocrinology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mce.2020.110801","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"110801"}}