{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:12:31Z","timestamp":1789168351810,"version":"build-2803163510"},"reference-count":115,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA232103"],"award-info":[{"award-number":["CA232103"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA069246"],"award-info":[{"award-number":["CA069246"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["CA179563"],"award-info":[{"award-number":["CA179563"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005294","name":"Massachusetts General Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016061","name":"Foundation De Drie Lichten","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006086","name":"Office of Strategic Coordination","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006086","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008358","name":"Hersenstichting","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008358","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002108","name":"Friedreich&apos;s Ataxia Research Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015326","name":"National Institutes of Health Common Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015326","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Neuroscience"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.mcn.2025.104051","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:57:18Z","timestamp":1759993038000},"page":"104051","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["AstroGreen transgenic mouse illuminates the trafficking of astrocyte-derived extracellular vesicles"],"prefix":"10.1016","volume":"135","author":[{"given":"Lisa","family":"Nieland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwina Abou","family":"Haidar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rufino-Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa","family":"Prabhakar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youssef","family":"Samaha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"Breyne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis K.","family":"Fordjour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saumya","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marike L.D.","family":"Broekman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Gould","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xandra O.","family":"Breakefield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik R.","family":"Abels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcn.2025.104051_bb0005","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s10571-016-0366-z","article-title":"Introduction to extracellular vesicles: biogenesis, RNA cargo selection, content, release, and uptake","volume":"36","author":"Abels","year":"2016","journal-title":"Cell. Mol. Neurobiol."},{"key":"10.1016\/j.mcn.2025.104051_bb0010","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1111\/j.1750-3639.2012.00592.x","article-title":"ALDH1A1 is a marker of astrocytic differentiation during brain development and correlates with better survival in glioblastoma patients","volume":"22","author":"Adam","year":"2012","journal-title":"Brain Pathol."},{"key":"10.1016\/j.mcn.2025.104051_bb0015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/sciadv.adi9156","article-title":"Endocytosis blocks the vesicular secretion of exosome marker proteins","volume":"10","author":"Ai","year":"2024","journal-title":"Sci. Adv."},{"key":"10.1016\/j.mcn.2025.104051_bb0020","article-title":"Syntenin and CD63 promote exosome biogenesis from the plasma membrane by blocking cargo endocytosis","author":"Ai","year":"2023","journal-title":"bioRxiv"},{"key":"10.1016\/j.mcn.2025.104051_bb0025","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1038\/s41467-019-14198-8","article-title":"Identification of region-specific astrocyte subtypes at single cell resolution","volume":"11","author":"Batiuk","year":"2020","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcn.2025.104051_bb0030","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1002\/glia.23520","article-title":"Astrocytes, the rising stars of the glioblastoma microenvironment","volume":"67","author":"Brandao","year":"2019","journal-title":"Glia"},{"key":"10.1016\/j.mcn.2025.104051_bb0035","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23158544","article-title":"Detailed characterization of small extracellular vesicles from different cell types based on tetraspanin composition by ExoView R100 platform","volume":"23","author":"Breitwieser","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0040","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22031365","article-title":"Brain-derived extracellular vesicles in health and disease: a methodological perspective","volume":"22","author":"Brenna","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0045","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-022-03440-7","article-title":"Exogenous loading of extracellular vesicles, virus-like particles, and lentiviral vectors with supercharged proteins","volume":"5","author":"Breyne","year":"2022","journal-title":"Commun. Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0050","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/s41582-018-0025-8","article-title":"Multidimensional communication in the microenvirons of glioblastoma","volume":"14","author":"Broekman","year":"2018","journal-title":"Nat. Rev. Neurol."},{"key":"10.1016\/j.mcn.2025.104051_bb0055","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.mcn.2005.12.006","article-title":"GFAP-positive progenitor cells produce neurons and oligodendrocytes throughout the CNS","volume":"31","author":"Casper","year":"2006","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0060","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.neuron.2017.06.029","article-title":"Neural circuit-specialized astrocytes: transcriptomic, proteomic, morphological, and functional evidence","volume":"95","author":"Chai","year":"2017","journal-title":"Neuron"},{"key":"10.1016\/j.mcn.2025.104051_bb0065","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1038\/s41419-018-0369-4","article-title":"TNFalpha and IL-1beta modify the miRNA cargo of astrocyte shed extracellular vesicles to regulate neurotrophic signaling in neurons","volume":"9","author":"Chaudhuri","year":"2018","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.mcn.2025.104051_bb0070","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12964-019-0494-4","article-title":"Extracellular vesicles originating from glioblastoma cells increase metalloproteinase release by astrocytes: the role of CD147 (EMMPRIN) and ionizing radiation","volume":"18","author":"Colangelo","year":"2020","journal-title":"Cell Commun. Signal"},{"key":"10.1016\/j.mcn.2025.104051_bb0075","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1038\/s41596-020-00466-1","article-title":"Isolation and characterization of extracellular vesicle subpopulations from tissues","volume":"16","author":"Crescitelli","year":"2021","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mcn.2025.104051_bb0080","doi-asserted-by":"crossref","DOI":"10.1038\/srep37246","article-title":"Digital detection of exosomes by interferometric imaging","volume":"6","author":"Daaboul","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0085","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1038\/s41596-022-00719-1","article-title":"Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues","volume":"17","author":"D\u2019Acunzo","year":"2022","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mcn.2025.104051_bb0090","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/glia.23708","article-title":"Stimulus-dependent modifications in astrocyte-derived extracellular vesicle cargo regulate neuronal excitability","volume":"68","author":"Datta Chaudhuri","year":"2020","journal-title":"Glia"},{"key":"10.1016\/j.mcn.2025.104051_bb0095","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.4103\/1673-5374.306064","article-title":"Astrocytes: a double-edged sword in neurodegenerative diseases","volume":"16","author":"Ding","year":"2021","journal-title":"Neural Regen. Res."},{"key":"10.1016\/j.mcn.2025.104051_bb0100","doi-asserted-by":"crossref","DOI":"10.3390\/cells8070727","article-title":"Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis","volume":"8","author":"Doyle","year":"2019","journal-title":"Cells"},{"key":"10.1016\/j.mcn.2025.104051_bb0105","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2017.00910","article-title":"ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes","volume":"8","author":"Drago","year":"2017","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.mcn.2025.104051_bb0110","doi-asserted-by":"crossref","first-page":"5272","DOI":"10.1038\/s41598-024-55983-w","article-title":"Elevation of astrocyte-derived extracellular vesicles over the first month post-stroke in humans","volume":"14","author":"Edwardson","year":"2024","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0115","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/s41593-020-00783-4","article-title":"Reactive astrocyte nomenclature, definitions, and future directions","volume":"24","author":"Escartin","year":"2021","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0120","doi-asserted-by":"crossref","first-page":"20121","DOI":"10.1074\/jbc.273.32.20121","article-title":"Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes","volume":"273","author":"Escola","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mcn.2025.104051_bb0125","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12352","article-title":"Differential proteomics argues against a general role for CD9, CD81 or CD63 in the sorting of proteins into extracellular vesicles","volume":"12","author":"Fan","year":"2023","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0130","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2022.102394","article-title":"A shared, stochastic pathway mediates exosome protein budding along plasma and endosome membranes","volume":"298","author":"Fordjour","year":"2022","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mcn.2025.104051_bb0135","article-title":"Exomap1 mouse: a transgenic model for in vivo studies of exosome biology","author":"Fordjour","year":"2023","journal-title":"bioRxiv"},{"key":"10.1016\/j.mcn.2025.104051_bb0140","doi-asserted-by":"crossref","first-page":"182","DOI":"10.3389\/fncel.2013.00182","article-title":"Extracellular vesicles as mediators of neuron-glia communication","volume":"7","author":"Fruhbeis","year":"2013","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0145","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0098186","article-title":"Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs","volume":"9","author":"Gagnon","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.mcn.2025.104051_bb0150","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13024-016-0108-1","article-title":"Enrichment of extracellular vesicles from tissues of the central nervous system by PROSPR","volume":"11","author":"Gallart-Palau","year":"2016","journal-title":"Mol. Neurodegener."},{"key":"10.1016\/j.mcn.2025.104051_bb0155","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1016\/j.celrep.2020.01.089","article-title":"Gliomas interact with non-glioma brain cells via extracellular vesicles","volume":"30","author":"Gao","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0160","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2020.568889","article-title":"The function of astrocyte mediated extracellular vesicles in central nervous system diseases","volume":"8","author":"Gharbi","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0165","doi-asserted-by":"crossref","first-page":"115","DOI":"10.4103\/2347-8659.153979","article-title":"Astrocytes: everything but the glue","volume":"2","author":"Gonzalez-Perez","year":"2015","journal-title":"Neuroimmunol. Neuroinflamm."},{"key":"10.1016\/j.mcn.2025.104051_bb0170","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24021599","article-title":"Astrocytic neuroimmunological roles interacting with microglial cells in neurodegenerative diseases","volume":"24","author":"Gotoh","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0175","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1523\/JNEUROSCI.3095-08.2009","article-title":"Pten deletion in adult neural stem\/progenitor cells enhances constitutive neurogenesis","volume":"29","author":"Gregorian","year":"2009","journal-title":"J. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0180","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2021.100838","article-title":"Choice of selectable marker affects recombinant protein expression in cells and exosomes","volume":"297","author":"Guo","year":"2021","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mcn.2025.104051_bb0185","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/s00418-008-0412-0","article-title":"The art of cellular communication: tunneling nanotubes bridge the divide","volume":"129","author":"Gurke","year":"2008","journal-title":"Histochem. Cell Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0190","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/s12929-024-01084-9","article-title":"CD81-guided heterologous EVs present heterogeneous interactions with breast cancer cells","volume":"31","author":"Gurrieri","year":"2024","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0195","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1084\/jem.20180200","article-title":"Astrocytes and microglia: models and tools","volume":"216","author":"Guttenplan","year":"2019","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.mcn.2025.104051_bb0200","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1038\/s41593-020-0624-8","article-title":"Disease-associated astrocytes in Alzheimer\u2019s disease and aging","volume":"23","author":"Habib","year":"2020","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0205","doi-asserted-by":"crossref","first-page":"4566","DOI":"10.1007\/s12035-018-1385-1","article-title":"Extracellular vesicles released by glioblastoma cells stimulate normal astrocytes to acquire a tumor-supportive phenotype via p53 and MYC signaling pathways","volume":"56","author":"Hallal","year":"2019","journal-title":"Mol. Neurobiol."},{"key":"10.1016\/j.mcn.2025.104051_bb0210","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v2i0.22859","article-title":"ISEV position paper: extracellular vesicle RNA analysis and bioinformatics","volume":"2","author":"Hill","year":"2013","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0215","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.ceb.2015.02.004","article-title":"Glial fibrillary acidic protein (GFAP) and the astrocyte intermediate filament system in diseases of the central nervous system","volume":"32","author":"Hol","year":"2015","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0220","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2020.1785746","article-title":"Influence of species and processing parameters on recovery and content of brain tissue-derived extracellular vesicles","volume":"9","author":"Huang","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0225","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.3233\/JAD-220322","article-title":"Brain tissue-derived extracellular vesicles in Alzheimer\u2019s disease display altered key protein levels including cell type-specific markers","volume":"90","author":"Huang","year":"2022","journal-title":"J. Alzheimers Dis."},{"key":"10.1016\/j.mcn.2025.104051_bb0230","article-title":"Towards a human brain EV atlas: characteristics of EVs from different brain regions, including small RNA and protein profiles","author":"Huang","year":"2023","journal-title":"bioRxiv"},{"key":"10.1016\/j.mcn.2025.104051_bb0235","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1007\/s11064-022-03699-6","article-title":"Ass pathology and neuron-glia interactions: a synaptocentric view","volume":"48","author":"Huffels","year":"2023","journal-title":"Neurochem. Res."},{"key":"10.1016\/j.mcn.2025.104051_bb0240","doi-asserted-by":"crossref","DOI":"10.3390\/biom11091361","article-title":"Beyond the GFAP-astrocyte protein markers in the brain","volume":"11","author":"Jurga","year":"2021","journal-title":"Biomolecules"},{"key":"10.1016\/j.mcn.2025.104051_bb0245","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1146\/annurev-neuro-070918-050443","article-title":"The emerging nature of astrocyte diversity","volume":"42","author":"Khakh","year":"2019","journal-title":"Annu. Rev. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0250","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/nyas.14977","article-title":"Astrocytic contributions to Huntington\u2019s disease pathophysiology","volume":"1522","author":"Khakh","year":"2023","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0255","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.neuint.2003.08.015","article-title":"The problem of astrocyte identity","volume":"45","author":"Kimelberg","year":"2004","journal-title":"Neurochem. Int."},{"key":"10.1016\/j.mcn.2025.104051_bb0260","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.nurt.2010.07.006","article-title":"Functions of astrocytes and their potential as therapeutic targets","volume":"7","author":"Kimelberg","year":"2010","journal-title":"Neurotherapeutics"},{"key":"10.1016\/j.mcn.2025.104051_bb0265","article-title":"Glioblastoma extracellular vesicles influence glial cell hyaluronic acid deposition to promote invasiveness","volume":"5","author":"Koessinger","year":"2023","journal-title":"Neurooncol. Adv."},{"key":"10.1016\/j.mcn.2025.104051_bb0270","doi-asserted-by":"crossref","DOI":"10.1038\/s41573-022-00390-x","article-title":"Function and therapeutic value of astrocytes in neurological diseases","author":"Lee","year":"2022","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.mcn.2025.104051_bb0275","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.1015841","article-title":"Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo","volume":"10","author":"Li","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0280","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12027","article-title":"Morphine-mediated release of miR-138 in astrocyte-derived extracellular vesicles promotes microglial activation","volume":"10","author":"Liao","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0285","doi-asserted-by":"crossref","DOI":"10.3402\/jev.v3.26913","article-title":"Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles","volume":"3","author":"Lotvall","year":"2014","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0290","article-title":"A comprehensive meta-analysis of circulation miRNAs in glioma as potential diagnostic biomarker","volume":"13","author":"Ma","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.mcn.2025.104051_bb0295","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1038\/nn.2467","article-title":"A robust and high-throughput Cre reporting and characterization system for the whole mouse brain","volume":"13","author":"Madisen","year":"2010","journal-title":"Nat. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0300","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22147294","article-title":"Living proof of activity of extracellular vesicles in the central nervous system","volume":"22","author":"Mahjoum","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0305","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-021-24384-2","article-title":"Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9","volume":"12","author":"Mathieu","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcn.2025.104051_bb0310","doi-asserted-by":"crossref","first-page":"6206","DOI":"10.2174\/1381612823666170913164738","article-title":"The transport mechanism of extracellular vesicles at the blood-brain barrier","volume":"23","author":"Matsumoto","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"10.1016\/j.mcn.2025.104051_bb0315","doi-asserted-by":"crossref","DOI":"10.1002\/dvg.23369","article-title":"Reporter mice for isolating and auditing cell type-specific extracellular vesicles in vivo","volume":"58","author":"McCann","year":"2020","journal-title":"Genesis"},{"key":"10.1016\/j.mcn.2025.104051_bb0320","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1212\/WNL.0000000000005534","article-title":"Glial fibrillary acid protein: functions and involvement in disease","volume":"90","author":"McKeon","year":"2018","journal-title":"Neurology"},{"key":"10.1016\/j.mcn.2025.104051_bb0325","doi-asserted-by":"crossref","first-page":"4136","DOI":"10.1038\/s41467-019-11534-w","article-title":"Exosome reporter mice reveal the involvement of exosomes in mediating neuron to astroglia communication in the CNS","volume":"10","author":"Men","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcn.2025.104051_bb0330","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.pneurobio.2011.01.005","article-title":"GFAP in health and disease","volume":"93","author":"Middeldorp","year":"2011","journal-title":"Prog. Neurobiol."},{"key":"10.1016\/j.mcn.2025.104051_bb0335","doi-asserted-by":"crossref","DOI":"10.3390\/cells9122623","article-title":"Neuron-astrocyte interactions in Parkinson\u2019s disease","volume":"9","author":"Miyazaki","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.mcn.2025.104051_bb0340","doi-asserted-by":"crossref","first-page":"2369","DOI":"10.1007\/s00018-019-03071-y","article-title":"Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography","volume":"76","author":"Monguio-Tortajada","year":"2019","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0345","doi-asserted-by":"crossref","DOI":"10.3389\/fneur.2021.619626","article-title":"The many faces of astrocytes in Alzheimer\u2019s disease","volume":"12","author":"Monterey","year":"2021","journal-title":"Front. Neurol."},{"key":"10.1016\/j.mcn.2025.104051_bb0350","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1038\/s41598-019-39679-0","article-title":"A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles","volume":"9","author":"Neckles","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0355","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.tins.2020.10.014","article-title":"Extracellular vesicle-mediated bilateral communication between glioblastoma and astrocytes","volume":"44","author":"Nieland","year":"2021","journal-title":"Trends Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0360","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-04512-0","article-title":"A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles","volume":"12","author":"Norgard","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0365","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/s41580-020-0251-y","article-title":"RNA delivery by extracellular vesicles in mammalian cells and its applications","volume":"21","author":"O\u2019Brien","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb9000","article-title":"Engineering of CD63 enables selective extracellular vesicle cargo loading and enhanced payload delivery","author":"Obuchi","year":"2024","journal-title":"JEV"},{"key":"10.1016\/j.mcn.2025.104051_bb0370","article-title":"Glioblastoma multiforme-derived extracellular vesicles drive normal astrocytes towards a tumour-enhancing phenotype","volume":"373","author":"Oushy","year":"2018","journal-title":"Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci."},{"key":"10.1016\/j.mcn.2025.104051_bb0375","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.neuroscience.2018.04.003","article-title":"Cell-to-cell communication by extracellular vesicles: focus on microglia","volume":"405","author":"Paolicelli","year":"2019","journal-title":"Neuroscience"},{"key":"10.1016\/j.mcn.2025.104051_bb0380","doi-asserted-by":"crossref","first-page":"334","DOI":"10.2478\/s13380-012-0040-y","article-title":"Homeostatic function of astrocytes: Ca(2+) and Na(+) signalling","volume":"3","author":"Parpura","year":"2012","journal-title":"Transl. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0385","article-title":"Extracellular vesicles secreted by astroglial cells transport apolipoprotein D to neurons and mediate neuronal survival upon oxidative stress","volume":"12","author":"Pascua-Maestro","year":"2018","journal-title":"Front. Cell. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0390","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2021.108829","article-title":"Astrocyte-derived small extracellular vesicles promote synapse formation via fibulin-2-mediated TGF-beta signaling","volume":"34","author":"Patel","year":"2021","journal-title":"Cell Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0395","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1146\/annurev-biochem-013118-111902","article-title":"Exosomes","volume":"88","author":"Pegtel","year":"2019","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.mcn.2025.104051_bb0400","doi-asserted-by":"crossref","DOI":"10.1016\/j.yexcr.2019.06.019","article-title":"Astrocyte-derived exosomes suppress autophagy and ameliorate neuronal damage in experimental ischemic stroke","volume":"382","author":"Pei","year":"2019","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.mcn.2025.104051_bb0405","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1002\/path.1645","article-title":"Astrocyte intermediate filaments in CNS pathologies and regeneration","volume":"204","author":"Pekny","year":"2004","journal-title":"J. Pathol."},{"key":"10.1016\/j.mcn.2025.104051_bb0410","doi-asserted-by":"crossref","DOI":"10.1093\/brain\/awac222","article-title":"Astrocyte immunometabolic regulation of the tumour microenvironment drives glioblastoma pathogenicity","author":"Perelroizen","year":"2022","journal-title":"Brain"},{"key":"10.1016\/j.mcn.2025.104051_bb0415","doi-asserted-by":"crossref","first-page":"43108","DOI":"10.1074\/jbc.M112.404467","article-title":"The exosome secretory pathway transports amyloid precursor protein carboxyl-terminal fragments from the cell into the brain extracellular space","volume":"287","author":"Perez-Gonzalez","year":"2012","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mcn.2025.104051_bb0420","doi-asserted-by":"crossref","first-page":"11649","DOI":"10.1074\/jbc.M112.445304","article-title":"The intracellular interactome of tetraspanin-enriched microdomains reveals their function as sorting machineries toward exosomes","volume":"288","author":"Perez-Hernandez","year":"2013","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mcn.2025.104051_bb0425","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.4103\/1673-5374.300994","article-title":"Astrocyte-derived extracellular vesicles mediate intercellular communications of the neurogliovascular unit","volume":"16","author":"Pivoriunas","year":"2021","journal-title":"Neural Regen. Res."},{"key":"10.1016\/j.mcn.2025.104051_bb0430","doi-asserted-by":"crossref","DOI":"10.3390\/cells9071604","article-title":"The diversity of intermediate filaments in astrocytes","volume":"9","author":"Potokar","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.mcn.2025.104051_bb0435","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s12987-022-00359-3","article-title":"Extracellular vesicles through the blood-brain barrier: a review","volume":"19","author":"Ramos-Zaldivar","year":"2022","journal-title":"Fluids Barriers CNS"},{"key":"10.1016\/j.mcn.2025.104051_bb0440","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1093\/neuonc\/noae068","article-title":"Circulating extracellular vesicles as biomarker for diagnosis, prognosis, and monitoring in glioblastoma patients","volume":"26","author":"Ricklefs","year":"2024","journal-title":"Neuro-Oncology"},{"key":"10.1016\/j.mcn.2025.104051_bb0445","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001874","article-title":"Extracellular vesicle-mediated transfer of genetic information between the hematopoietic system and the brain in response to inflammation","volume":"12","author":"Ridder","year":"2014","journal-title":"PLoS Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0450","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.14336\/AD.2021.0608","article-title":"Astrocyte-derived extracellular vesicles (ADEVs): deciphering their influences in aging","volume":"12","author":"Rouillard","year":"2021","journal-title":"Aging Dis."},{"key":"10.1016\/j.mcn.2025.104051_bb0455","doi-asserted-by":"crossref","DOI":"10.1016\/j.biomaterials.2022.121366","article-title":"Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo","volume":"281","author":"Rufino-Ramos","year":"2022","journal-title":"Biomaterials"},{"key":"10.1016\/j.mcn.2025.104051_bb0460","doi-asserted-by":"crossref","first-page":"2220","DOI":"10.1016\/j.ymthe.2023.05.012","article-title":"Extracellular communication between brain cells through functional transfer of Cre mRNA mediated by extracellular vesicles","volume":"31","author":"Rufino-Ramos","year":"2023","journal-title":"Mol. Ther."},{"key":"10.1016\/j.mcn.2025.104051_bb0465","doi-asserted-by":"crossref","DOI":"10.3390\/cells9040851","article-title":"Targeting and crossing the blood-brain barrier with extracellular vesicles","volume":"9","author":"Saint-Pol","year":"2020","journal-title":"Cells"},{"key":"10.1016\/j.mcn.2025.104051_bb0470","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines10112952","article-title":"Astrocytes-derived small extracellular vesicles hinder glioma growth","volume":"10","author":"Serpe","year":"2022","journal-title":"Biomedicines"},{"key":"10.1016\/j.mcn.2025.104051_bb0475","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12130","article-title":"Quantification of protein cargo loading into engineered extracellular vesicles at single-vesicle and single-molecule resolution","volume":"10","author":"Silva","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0480","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1007\/978-1-61779-452-0_35","article-title":"Transgenic techniques for cell ablation or molecular deletion to investigate functions of astrocytes and other GFAP-expressing cell types","volume":"814","author":"Sofroniew","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0485","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1016\/j.it.2020.07.004","article-title":"Astrocyte reactivity: subtypes, states, and functions in CNS innate immunity","volume":"41","author":"Sofroniew","year":"2020","journal-title":"Trends Immunol."},{"key":"10.1016\/j.mcn.2025.104051_bb0490","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00401-009-0619-8","article-title":"Astrocytes: biology and pathology","volume":"119","author":"Sofroniew","year":"2010","journal-title":"Acta Neuropathol."},{"key":"10.1016\/j.mcn.2025.104051_bb0495","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12089","article-title":"Characterization of brain-derived extracellular vesicle lipids in Alzheimer\u2019s disease","volume":"10","author":"Su","year":"2021","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0500","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047311","article-title":"Role of gap junction protein connexin43 in astrogliosis induced by brain injury","volume":"7","author":"Theodoric","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.mcn.2025.104051_bb0505","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1083\/jcb.147.3.599","article-title":"Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73","volume":"147","author":"Thery","year":"1999","journal-title":"J. Cell Biol."},{"key":"10.1016\/j.mcn.2025.104051_bb0510","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Thery","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0515","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jconrel.2020.04.017","article-title":"Astrocyte-derived extracellular vesicles: neuroreparative properties and role in the pathogenesis of neurodegenerative disorders","volume":"323","author":"Upadhya","year":"2020","journal-title":"J. Control. Release"},{"key":"10.1016\/j.mcn.2025.104051_bb0520","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2017.1348885","article-title":"A rigorous method to enrich for exosomes from brain tissue","volume":"6","author":"Vella","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0525","doi-asserted-by":"crossref","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0530","doi-asserted-by":"crossref","DOI":"10.1038\/srep31172","article-title":"Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids","volume":"6","author":"Yoshimura","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcn.2025.104051_bb0535","doi-asserted-by":"crossref","DOI":"10.1242\/dmm.028779","article-title":"The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles","volume":"11","author":"Yoshimura","year":"2018","journal-title":"Dis. Model. Mech."},{"key":"10.1016\/j.mcn.2025.104051_bb0540","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2019.1706801","article-title":"Activated human astrocyte-derived extracellular vesicles modulate neuronal uptake, differentiation and firing","volume":"9","author":"You","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"10.1016\/j.mcn.2025.104051_bb0545","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/s41583-020-0264-8","article-title":"Improved tools to study astrocytes","volume":"21","author":"Yu","year":"2020","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.mcn.2025.104051_bb0550","doi-asserted-by":"crossref","DOI":"10.1016\/j.jneumeth.2021.109420","article-title":"Novel protocol for the isolation of highly purified neonatal murine microglia and astrocytes","volume":"366","author":"Zelenka","year":"2022","journal-title":"J. Neurosci. Methods"},{"key":"10.1016\/j.mcn.2025.104051_bb0555","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2020.101420","article-title":"Glioblastoma-derived extracellular vesicles facilitate transformation of astrocytes via reprogramming oncogenic metabolism","volume":"23","author":"Zeng","year":"2020","journal-title":"iScience"},{"key":"10.1016\/j.mcn.2025.104051_bb0560","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.neubiorev.2021.02.027","article-title":"Astrocyte-derived extracellular vesicles: a double-edged sword in central nervous system disorders","volume":"125","author":"Zhao","year":"2021","journal-title":"Neurosci. Biobehav. Rev."},{"key":"10.1016\/j.mcn.2025.104051_bb0565","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1002\/gene.10008","article-title":"hGFAP-cre transgenic mice for manipulation of glial and neuronal function in vivo","volume":"31","author":"Zhuo","year":"2001","journal-title":"Genesis"},{"key":"10.1016\/j.mcn.2025.104051_bb0570","doi-asserted-by":"crossref","DOI":"10.1080\/10717544.2022.2162161","article-title":"The cell type dependent sorting of CD9- and CD81 to extracellular vesicles can be exploited to convey tumor sensitive cargo to target cells","volume":"30","author":"Zuppone","year":"2023","journal-title":"Drug Deliv."}],"container-title":["Molecular and Cellular Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1044743125000612?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1044743125000612?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:01:51Z","timestamp":1780635711000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1044743125000612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":115,"alternative-id":["S1044743125000612"],"URL":"https:\/\/doi.org\/10.1016\/j.mcn.2025.104051","relation":{},"ISSN":["1044-7431"],"issn-type":[{"value":"1044-7431","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"AstroGreen transgenic mouse illuminates the trafficking of astrocyte-derived extracellular vesicles","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Neuroscience","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcn.2025.104051","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Inc.","name":"copyright","label":"Copyright"}],"article-number":"104051"}}