{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T23:23:24Z","timestamp":1774308204479,"version":"3.50.1"},"reference-count":48,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2004,12]]},"DOI":"10.1016\/j.mcp.2004.06.005","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T21:01:40Z","timestamp":1096059700000},"page":"389-398","source":"Crossref","is-referenced-by-count":25,"title":["Molecular identification of enteroviruses responsible for an outbreak of aseptic meningitis; implications in clinical practice and epidemiology"],"prefix":"10.1016","volume":"18","author":[{"given":"Nikolaos","family":"Siafakas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panayotis","family":"Markoulatos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stamatina","family":"Levidiotou-Stefanou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2004.06.005_bib1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0928-0197(98)00006-3","article-title":"Accomplishments and challenges in picornavirology as observed by a medical doctor","volume":"9","author":"Eggers","year":"1998","journal-title":"Clin Diagn Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib2","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1002\/(SICI)1096-9071(199906)58:2<178::AID-JMV13>3.0.CO;2-Q","article-title":"Specific detection of Echoviruses 22 and 23 in cell culture supernatants by RT-PCR","volume":"58","author":"Oberste","year":"1999","journal-title":"J Med Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib3","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1093\/clinids\/20.4.971","article-title":"Enteroviral infections of the central nervous system","volume":"20","author":"Rotbart","year":"1995","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.mcp.2004.06.005_bib4","series-title":"Textbook of pediatric infectious diseases","first-page":"1729","article-title":"Enteroviruses","author":"Cherry","year":"1987"},{"key":"10.1016\/j.mcp.2004.06.005_bib5","first-page":"114","article-title":"Enteroviruses in human disease","volume":"24","author":"Grist","year":"1978","journal-title":"Prog Med Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib6","series-title":"Virology","first-page":"655","article-title":"Enteroviruses: polioviruses, coxsackieviruses, echoviruses and newer enetroviruses","author":"Melnick","year":"1996"},{"key":"10.1016\/j.mcp.2004.06.005_bib7","first-page":"42","article-title":"A community and intensive care nursery outbreak of coxsackievirus B5 meningitis","volume":"127","author":"Swender","year":"1974","journal-title":"Am J Dis Child"},{"key":"10.1016\/j.mcp.2004.06.005_bib8","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1542\/peds.67.4.489","article-title":"Enterovirus 71 infection: report of an outbreak with two cases of paralysis and a review of the literature","volume":"67","author":"Chonmaitree","year":"1981","journal-title":"Pediatrics"},{"key":"10.1016\/j.mcp.2004.06.005_bib9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/S0168-1702(00)00162-3","article-title":"Genetic analysis of enterovirus 71 isolated from fatal and non-fatal cases of hand-foot-and-mouth-disease during an epidemic in Taiwan, 1998","volume":"68","author":"Shih","year":"2000","journal-title":"Virus Res"},{"key":"10.1016\/j.mcp.2004.06.005_bib10","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1093\/oxfordjournals.aje.a112688","article-title":"Non-polio enterovirus activity in Wisconsin based on a 20-year experience in a diagnostic virology laboratory","volume":"109","author":"Nelson","year":"1979","journal-title":"Am J Epidemiol"},{"key":"10.1016\/j.mcp.2004.06.005_bib11","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1001\/jama.1982.03320380035025","article-title":"The clinical relevance of \u2018CSF viral culture\u2019. A two-year experience with aseptic meningitis in Rochester, NY","volume":"247","author":"Chonmaitree","year":"1982","journal-title":"JAMA"},{"key":"10.1016\/j.mcp.2004.06.005_bib12","first-page":"454","article-title":"The importance of the virology laboratory in the diagnosis and management of viral meningitis","volume":"141","author":"Wildin","year":"1987","journal-title":"Am J Dis Child"},{"key":"10.1016\/j.mcp.2004.06.005_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/CMR.2.1.1","article-title":"Role of the virology laboratory in diagnosis and management of patients with central nervous system disease","volume":"2","author":"Chonmaitree","year":"1989","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.mcp.2004.06.005_bib14","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0065-3527(08)60089-5","article-title":"Biology of Coxsackie A viruses","volume":"42","author":"Hyypi\u00e4","year":"1993","journal-title":"Adv Vir Res"},{"key":"10.1016\/j.mcp.2004.06.005_bib15","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1099\/0022-1317-66-12-2627","article-title":"Oligonucleotide fingerprint analysis of coxsackievirus A10 isolated in Japan","volume":"66","author":"Kamahora","year":"1985","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib16","series-title":"Viral infections of humans","first-page":"583","article-title":"Poliovirus and other enteroviruses","author":"Melnick","year":"1997"},{"issue":"1","key":"10.1016\/j.mcp.2004.06.005_bib17","volume":"2","author":"World Health Organisation","year":"1996","journal-title":"Polio Lab Network"},{"key":"10.1016\/j.mcp.2004.06.005_bib18","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-0934(94)00173-E","article-title":"New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay","volume":"53","author":"Casas","year":"1995","journal-title":"J Virol Methods"},{"key":"10.1016\/j.mcp.2004.06.005_bib19","doi-asserted-by":"crossref","first-page":"4426","DOI":"10.1128\/JCM.39.12.4426-4432.2001","article-title":"Laboratory diagnosis of common herpesvirus infections of the central nervous system by a multiplex PCR assay","volume":"39","author":"Markoulatos","year":"2001","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2004.06.005_bib20","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/1098-2825(2000)14:4<157::AID-JCLA4>3.0.CO;2-P","article-title":"Isolation of polioviruses and other enteroviruses in South Greece between 1994 and 1998","volume":"14","author":"Siafakas","year":"2000","journal-title":"J Clin Lab Anal"},{"key":"10.1016\/j.mcp.2004.06.005_bib21","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1006\/mcpr.2001.0380","article-title":"High sequence divergence in the 5\u2032 non-coding region of reference Coxsackie B and ECHO viral strains and clinical isolates revealed by restriction fragment length polymorphism analysis","volume":"15","author":"Georgopoulou","year":"2001","journal-title":"Mol Cell Probes"},{"key":"10.1016\/j.mcp.2004.06.005_bib22","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1002\/jcla.7","article-title":"Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis","volume":"15","author":"Siafakas","year":"2001","journal-title":"J Clin Lab Anal"},{"key":"10.1016\/j.mcp.2004.06.005_bib23","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jcla.2063","article-title":"Variability in molecular typing of Coxsackie A viruses by RFLP analysis and sequencing","volume":"16","author":"Siafakas","year":"2002","journal-title":"J Clin Lab Anal"},{"key":"10.1016\/j.mcp.2004.06.005_bib24","doi-asserted-by":"crossref","first-page":"4337","DOI":"10.1128\/JCM.38.12.4337-4342.2000","article-title":"Improved genotyping vaccine and wild-type poliovirus strains by restriction fragment length polymorphism analysis: clinical diagnostic implications","volume":"38","author":"Georgopoulou","year":"2000","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2004.06.005_bib25","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0890-8508(03)00029-X","article-title":"Molecular sub-grouping of enterovirus reference and wild-type strains into distinct genetic clusters using a simple RFLP assay","volume":"17","author":"Siafakas","year":"2003","journal-title":"Mol Cell Probes"},{"key":"10.1016\/j.mcp.2004.06.005_bib26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1099\/0022-1317-78-1-1","article-title":"Classification of enteroviruses based on molecular and biological properties","volume":"78","author":"Hyypi\u00e4","year":"1997","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib27","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1099\/0022-1317-77-8-1699","article-title":"phylogenetic clustering of enteroviruses","volume":"77","author":"P\u00f6yry","year":"1996","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib28","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1006\/viro.1995.1450","article-title":"Molecular comparison of coxsackie A virus serotypes","volume":"212","author":"Pulli","year":"1995","journal-title":"Virology"},{"key":"10.1016\/j.mcp.2004.06.005_bib29","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1007\/s00239-002-2359-4","article-title":"Molecular classification of coxsackie A viruses on the basis of the 5\u2032-UTR: structural and evolutionary aspects","volume":"55","author":"Siafakas","year":"2002","journal-title":"J Mol Evol"},{"key":"10.1016\/j.mcp.2004.06.005_bib30","first-page":"543","article-title":"Lyophilized combination pools of enterovirus equine antisera: new LBM pools prepared from reserves of antisera stored frozen for two decades","volume":"63","author":"Melnick","year":"1985","journal-title":"Bull WHO"},{"key":"10.1016\/j.mcp.2004.06.005_bib31","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1128\/CMR.11.1.202","article-title":"European Union concerted action on virus meningitis and encephalitis, molecular typing of enteroviruses: current status and future requirements","volume":"11","author":"Muir","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.mcp.2004.06.005_bib32","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1002\/(SICI)1096-9071(199907)58:3<304::AID-JMV18>3.0.CO;2-U","article-title":"A study of enterovirus isolations in Glasgow from 1977 to 1997","volume":"58","author":"Nairn","year":"1999","journal-title":"J Med Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib33","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/jmv.2039","article-title":"Circulation of enteroviruses and persistence of meningitis cases in the winter of 1999\u20132000","volume":"65","author":"Chambon","year":"2001","journal-title":"J Med Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib34","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1002\/jmv.10412","article-title":"Molecular typing and epidemiology of enteroviruses identified from an outbreak of aseptic meningitis in Belgium during the summer of 2000","volume":"70","author":"Thoelen","year":"2003","journal-title":"J Med Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib35","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1128\/JCM.42.3.963-971.2004","article-title":"Analysis of the serotype and genotype correlation of VP1 and the 5\u2032 noncoding region in an epidemiological survey of the human enterovirus B species","volume":"42","author":"Thoelen","year":"2004","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2004.06.005_bib36","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1128\/JCM.40.3.1069-1070.2002","article-title":"Comparison of molecular and conventional methods for typing of enteroviral isolates","volume":"40","author":"Manayani","year":"2002","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2004.06.005_bib37","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1128\/CMR.9.1.18","article-title":"Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates","volume":"9","author":"Fredricks","year":"1996","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.mcp.2004.06.005_bib38","doi-asserted-by":"crossref","first-page":"4940","DOI":"10.1128\/JVI.76.10.4940-4949.2002","article-title":"Molecular epidemiology of echovirus 30: temporal circulation and prevalence of genetic lineages","volume":"76","author":"Palacios","year":"2002","journal-title":"J Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib39","doi-asserted-by":"crossref","first-page":"8741","DOI":"10.1128\/JVI.73.10.8741-8749.1999","article-title":"Evidence of recombination among enteroviruses","volume":"73","author":"Santti","year":"1999","journal-title":"J Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib40","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0168-1702(02)00019-9","article-title":"Molecular analysis of the echovirus 18 prototype: evidence of interserotypic recombination with echovirus 9","volume":"85","author":"Andersson","year":"2002","journal-title":"Virus Res"},{"key":"10.1016\/j.mcp.2004.06.005_bib41","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.1099\/0022-1317-83-9-2193","article-title":"Natural genetic recombination between co-circulating heterotypic enteroviruses","volume":"83","author":"Oprisan","year":"2002","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib42","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1099\/vir.0.18971-0","article-title":"Evolution of the genome of Human enterovirus B: incongruence between phylogenies of the VP1 and the 3CD regions indicates frequent recombination within the species","volume":"84","author":"Lindberg","year":"2003","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib43","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1099\/vir.0.18708-0","article-title":"Characterization of a recombinant type 3\/type 2 poliovirus isolated from a healthy vaccine and containing a chimeric capsid protein VP1","volume":"84","author":"Blomqvist","year":"2003","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib44","doi-asserted-by":"crossref","first-page":"5740","DOI":"10.1128\/JVI.75.13.5740-5751.2001","article-title":"Genomic features of intertypic recombinant sabin poliovirus strains excreted by primary vaccines","volume":"75","author":"Cuervo","year":"2001","journal-title":"J Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib45","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1006\/viro.1993.1468","article-title":"Polioviruses with natural recombinant genomes isolated from vaccine-associated paralytic poliomyelitis","volume":"196","author":"Furione","year":"1993","journal-title":"Virology"},{"key":"10.1016\/j.mcp.2004.06.005_bib46","doi-asserted-by":"crossref","first-page":"8089","DOI":"10.1128\/JVI.68.12.8089-8101.1994","article-title":"High diversity of poliovirus strains isolated from the central nervous system from patients with vaccine-associated paralytic poliomyelitis","volume":"68","author":"Georgescu","year":"1994","journal-title":"J Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib47","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1099\/0022-1317-70-11-2943","article-title":"The complete nucleotide sequence of coxsackievirus A21","volume":"70","author":"Hughes","year":"1989","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2004.06.005_bib48","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/0168-1702(95)00055-U","article-title":"Genotypic variation in coxsackievirus B5 isolates from three different outbreaks in the United States","volume":"38","author":"Kopecka","year":"1995","journal-title":"Virus Res"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850804000623?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850804000623?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T10:45:55Z","timestamp":1682765155000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850804000623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["S0890850804000623"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2004.06.005","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}