{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T05:43:42Z","timestamp":1768715022275,"version":"3.49.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2005,2,1]],"date-time":"2005-02-01T00:00:00Z","timestamp":1107216000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2005,2]]},"DOI":"10.1016\/j.mcp.2004.08.003","type":"journal-article","created":{"date-parts":[[2004,11,16]],"date-time":"2004-11-16T19:05:09Z","timestamp":1100631909000},"page":"21-25","source":"Crossref","is-referenced-by-count":14,"title":["Specific identification of Egyptian Biomphalaria species and possible hybrids using the polymerase chain reaction based on nuclear and mitochondrial loci"],"prefix":"10.1016","volume":"19","author":[{"given":"Wael M.","family":"Lotfy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall J.","family":"DeJong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandee S.","family":"Black","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric S.","family":"Loker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2004.08.003_bib1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1136\/bmj.1.2557.16-a","article-title":"Note on the presence of bilharzia haematobia in Egyptian mummies of the twentieth dynasty","volume":"1","author":"Ruffer","year":"1910","journal-title":"Br Med J"},{"key":"10.1016\/j.mcp.2004.08.003_bib2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0035-9203(00)90252-9","article-title":"Tropical diseases in ancient Egypt","volume":"94","author":"Nunn","year":"2000","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2004.08.003_bib3","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/oxfordjournals.jhered.a111210","article-title":"Population genetics of Egyptian Biomphalaria alexandrina (Gastropoda, Planorbidae)","volume":"83","author":"Vrijenhoek","year":"1992","journal-title":"J Hered"},{"key":"10.1016\/j.mcp.2004.08.003_bib4","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0035-9203(82)90167-5","article-title":"Introduction of Biomphalaria glabrata to Egypt and other African countries","volume":"76","author":"Pfl\u00fcger","year":"1982","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2004.08.003_bib5","first-page":"191","article-title":"Biomphalaria glabrata: a new threat for schistosomiasis transmission in Egypt","volume":"26","author":"Yousif","year":"1996","journal-title":"J Egypt Soc Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib6","first-page":"569","article-title":"Invasion of the Nile Valley in Egypt by a hybrid of Biomphalaria glabrata and Biomphalaria alexandrina, snail vectors of Schistosoma mansoni","volume":"28","author":"Yousif","year":"1998","journal-title":"J Egypt Soc Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib7","first-page":"863","article-title":"Compatibility of Biomphalaria alexandrina, Biomphalaria glabrata and a hybrid of both to seven strains of Schistosoma mansoni from Egypt","volume":"28","author":"Yousif","year":"1998","journal-title":"J Egypt Soc Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib8","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1093\/mollus\/65.1.133","article-title":"Molecular characterisation of Biomphalaria spp. in Egypt","volume":"65","author":"Kristensen","year":"1999","journal-title":"J Moll Stud"},{"key":"10.1016\/j.mcp.2004.08.003_bib9","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1017\/S0007485300054250","article-title":"Molecular markers in entomology","volume":"88","author":"Loxdale","year":"1998","journal-title":"Bull Entomol Res"},{"issue":"suppl. 1","key":"10.1016\/j.mcp.2004.08.003_bib10","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1590\/S0074-02761998000700015","article-title":"Molecular characterisation of intermediate snail hosts and the search for resistance genes","volume":"93","author":"Rollinson","year":"1998","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib11","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1006\/expr.1998.4286","article-title":"Biomphalaria spp: identification of the intermediate snail hosts of Schistosoma mansoni by polymerase chain reaction amplification and restriction enzyme digestion of the ribosomal RNA gene intergenic spacer","volume":"89","author":"Vidigal","year":"1998","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib12","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1590\/S0074-02761998000700025","article-title":"Molecular study of similar Biomphalaria species","volume":"93","author":"Spatz","year":"1998","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib13","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1590\/S0074-02761998000700039","article-title":"Molecular identification of similar species of the genus Biomphalaria (Mollusca: Planorbidae) determined by a PCR-RFLP","volume":"93","author":"Caldeira","year":"1998","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib14","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1093\/mollus\/65.2.143","article-title":"Study of Biomphalaria tenagophila, B. t. guaibensis and B. occidentalis by polymerase chain reaction amplification and restriction enzyme digestion of the ribosomal RNA gene intergenic spacer","volume":"65","author":"Spatz","year":"1999","journal-title":"J Moll Studies"},{"key":"10.1016\/j.mcp.2004.08.003_bib15","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1590\/S0074-02762000000100009","article-title":"Further studies on the molecular systematics of Biomphalaria snails from Brazil","volume":"95","author":"Vidigal","year":"2000","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib16","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1590\/S0074-02762000000200007","article-title":"Identification of planorbids from Venezuela by polymerase chain reaction amplification and restriction fragment length polymorphism of ITS of the RNA ribosomal gene","volume":"95","author":"Caldeira","year":"2000","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib17","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1590\/S0074-02762000000600010","article-title":"Characterization of Biomphalaria orbignyi, B. peregrina and B. oligoza by polymerase chain reaction and restriction enzyme digestion of the internal transcribed spacer region of the RNA ribosomal gene","volume":"95","author":"Spatz","year":"2000","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib18","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1590\/S0074-02762001000500013","article-title":"Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer","volume":"96","author":"Vidigal","year":"2001","journal-title":"Mem Inst Oswaldo Cruz"},{"issue":"suppl","key":"10.1016\/j.mcp.2004.08.003_bib19","doi-asserted-by":"crossref","first-page":"S197","DOI":"10.1017\/S0031182001008058","article-title":"Genetic variability and molecular identification of Brazilian Biomphalaria species (Mollusca: Planorbidae)","volume":"123","author":"Carvalho","year":"2001","journal-title":"Parasitol"},{"issue":"suppl. 1","key":"10.1016\/j.mcp.2004.08.003_bib20","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1590\/S0074-02762002000900011","article-title":"Polymerase chain reaction and restriction fragment length polymorphism of cytochrome oxidase subunit I used for differentiation of Brazilian Biomphalaria species intermediate host of Schistosoma mansoni","volume":"97","author":"Vidigal","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib21","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1590\/S0074-02762002000700012","article-title":"Morphological and polymerase chain reaction\u2013restriction fragment length polymorphism characterization of Biomphalaria kuhniana and Biomphalaria amazonica from Colombia","volume":"97","author":"Velasquez","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib22","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2144\/00291bm09","article-title":"Preparation of PCR-Quality mouse genomic DNA with hot sodium hydroxide and Tris (Hot-SHOT)","volume":"29","author":"Truett","year":"2000","journal-title":"BioTechniques"},{"key":"10.1016\/j.mcp.2004.08.003_bib23","doi-asserted-by":"crossref","first-page":"3041","DOI":"10.1046\/j.1365-294X.2003.01977.x","article-title":"Phylogeography of Biomphalaria glabrata and B. pfeifferi, important intermediate hosts of Schistosoma mansoni in the new and old world tropics","volume":"12","author":"DeJong","year":"2003","journal-title":"Mol Ecol"},{"key":"10.1016\/j.mcp.2004.08.003_bib24","doi-asserted-by":"crossref","first-page":"2225","DOI":"10.1093\/oxfordjournals.molbev.a003769","article-title":"Evolutionary relationships and biogeography of Biomphalaria (Gastropoda: Planorbidae) with implications regarding its role as host of the human bloodfluke, Schistosoma mansoni","volume":"18","author":"DeJong","year":"2001","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.mcp.2004.08.003_bib25","first-page":"105","article-title":"Estado atual da sistem\u00e1tica dos planorb\u00eddeos brasileiros","volume":"55","author":"Paraense","year":"1975","journal-title":"Arq Mus Nac Rio de Janeiro"},{"key":"10.1016\/j.mcp.2004.08.003_bib26","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1590\/S0074-02761981000200011","article-title":"Biomphalaria occidentalis sp.n. from South America (Mollusca, Basommatophora, Pulmonata)","volume":"76","author":"Paraense","year":"1981","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib27","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1590\/S0074-02761984000400012","article-title":"Biomphalaria tenagophila guaibensis sp.n. from Southern Brazil and Uruguay (Pulmonata: Planorbidae). I. Morphology","volume":"79","author":"Paraense","year":"1984","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0074-02761988000100001","article-title":"Biomphalaria kuhniana (Clessin, 1883), planorbid mollusc from South America","volume":"83","author":"Paraense","year":"1988","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib29","first-page":"115","article-title":"Biomphalaria amazonica and B. cousini, two new species of neotropical planorbid mollusks","volume":"26","author":"Paraense","year":"1966","journal-title":"Rev Bras Biol"},{"issue":"suppl. 1","key":"10.1016\/j.mcp.2004.08.003_bib30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1590\/S0074-02762002000900019","article-title":"A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni","volume":"97","author":"Vidigal","year":"2002","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2004.08.003_bib31","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S1471-4922(00)01944-9","article-title":"The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study","volume":"17","author":"Anderson","year":"2001","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib32","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0020-7519(01)00357-5","article-title":"Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer","volume":"32","author":"Blouin","year":"2002","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib33","doi-asserted-by":"crossref","DOI":"10.1645\/GE-284R","article-title":"The mitochondrial genome of Biomphalaria glabrata (Gastropoda: Basommatophora), intermediate host of Schistosoma mansoni","author":"DeJong","year":"2004","journal-title":"J Parasitol"},{"key":"10.1016\/j.mcp.2004.08.003_bib34","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/S0169-5347(01)02151-6","article-title":"Mitochondrial pseudogenes: evolution's misplaced witnesses","volume":"16","author":"Bensasson","year":"2001","journal-title":"Trends Ecol Evol"},{"key":"10.1016\/j.mcp.2004.08.003_bib35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0022-2836(72)90521-9","article-title":"A comparison of the ribosomal DNAs of Xenopus leavis and Xenopus mulleri: the evolution of tandem genes","volume":"63","author":"Brown","year":"1972","journal-title":"J Mol Biol"},{"key":"10.1016\/j.mcp.2004.08.003_bib36","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1111\/j.1365-294X.2004.02167.x","article-title":"Molecular evidence for the hybrid origin of species in the soft coral genus Alcyonium (Cnidaria: Anthozoa: Octocorallia)","volume":"13","author":"McFadden","year":"2004","journal-title":"Mol Ecol"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850804000775?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850804000775?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T03:54:08Z","timestamp":1585972448000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850804000775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["S0890850804000775"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2004.08.003","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2005,2]]}}}