{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:51:05Z","timestamp":1789195865366,"version":"build-2803163510"},"reference-count":36,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1016\/j.mcp.2010.04.003","type":"journal-article","created":{"date-parts":[[2010,4,22]],"date-time":"2010-04-22T08:36:21Z","timestamp":1271925381000},"page":"219-228","source":"Crossref","is-referenced-by-count":38,"title":["Rapid identification of vector-borne flaviviruses by mass spectrometry"],"prefix":"10.1016","volume":"24","author":[{"given":"Rebecca J.","family":"Grant-Klein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carson D.","family":"Baldwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Turell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia A.","family":"Rossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Lovari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris D.","family":"Crowder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather E.","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan A.","family":"Rounds","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Eshoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence B.","family":"Blyn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Ecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rangarajan","family":"Sampath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris A.","family":"Whitehouse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2010.04.003_bib1","doi-asserted-by":"crossref","first-page":"688","DOI":"10.4269\/ajtmh.2008.79.688","article-title":"Size alters susceptibility of vectors to dengue virus infection and dissemination","volume":"79","author":"Alto","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib2","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.jviromet.2006.03.009","article-title":"A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses","volume":"135","author":"Ayers","year":"2006","journal-title":"J Virol Methods"},{"key":"10.1016\/j.mcp.2010.04.003_bib3","doi-asserted-by":"crossref","first-page":"971","DOI":"10.4269\/ajtmh.2008.79.971","article-title":"Stable prevalence of Powassan virus in Ixodes scapularis in a northern Wisconsin focus","volume":"79","author":"Brackney","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib4","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.2144\/96206st01","article-title":"Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping","volume":"20","author":"Brownstein","year":"1996","journal-title":"Biotechniques"},{"key":"10.1016\/j.mcp.2010.04.003_bib5","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1603\/033.047.0112","article-title":"Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens","volume":"47","author":"Crowder","year":"2009","journal-title":"J Med Entomol"},{"key":"10.1016\/j.mcp.2010.04.003_bib6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1146\/annurev-ento-112408-085446","article-title":"Update on Powassan virus: emergence of a North American tick-borne flavivirus","volume":"55","author":"Ebel","year":"2010","journal-title":"Annu Rev Entomol"},{"key":"10.1016\/j.mcp.2010.04.003_bib7","first-page":"341","article-title":"The Ibis T5000 universal biosensor \u2013 an automated platform for pathogen identification and strain typing","volume":"11","author":"Ecker","year":"2006","journal-title":"JALA"},{"key":"10.1016\/j.mcp.2010.04.003_bib8","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1038\/nrmicro1918","article-title":"Ibis T5000: a universal biosensor approach for microbiology","volume":"6","author":"Ecker","year":"2008","journal-title":"Nat Rev Microbiol"},{"issue":"Pt 1","key":"10.1016\/j.mcp.2010.04.003_bib9","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/0022-1317-80-1-179","article-title":"Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia","volume":"80","author":"Ecker","year":"1999","journal-title":"J Gen Virol"},{"key":"10.1016\/j.mcp.2010.04.003_bib10","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.virol.2007.06.016","article-title":"Direct broad-range detection of alphaviruses in mosquito extracts","volume":"368","author":"Eshoo","year":"2007","journal-title":"Virology"},{"key":"10.1016\/j.mcp.2010.04.003_bib11","doi-asserted-by":"crossref","first-page":"2677","DOI":"10.1128\/JCM.00971-06","article-title":"Mass spectrometry for species or strain identification after culture or without culture: past, present, and future","volume":"44","author":"Fox","year":"2006","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2010.04.003_bib12","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.4269\/ajtmh.1983.32.1154","article-title":"The effect of laboratory colonization on the vector-pathogen interactions of Egyptian Culex pipiens and Rift Valley fever virus","volume":"32","author":"Gargan","year":"1983","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib13","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.jviromet.2005.04.006","article-title":"Rapid subgroup identification of the flaviviruses using degenerate primer E-gene RT-PCR and site specific restriction enzyme analysis","volume":"128","author":"Gaunt","year":"2005","journal-title":"J Virol Methods"},{"key":"10.1016\/j.mcp.2010.04.003_bib14","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/S0140-6736(08)60238-X","article-title":"Pathogenic flaviviruses","volume":"371","author":"Gould","year":"2008","journal-title":"Lancet"},{"key":"10.1016\/j.mcp.2010.04.003_bib15","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0166-3542(02)00206-1","article-title":"Tick-borne encephalitis","volume":"57","author":"Gritsun","year":"2003","journal-title":"Antivir Res"},{"key":"10.1016\/j.mcp.2010.04.003_bib16","series-title":"Fields virology","first-page":"1153","article-title":"Flaviviruses","author":"Gubler","year":"2007"},{"key":"10.1016\/j.mcp.2010.04.003_bib17","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1089\/vbz.2008.0022","article-title":"Increased recognition of Powassan encephalitis in the United States, 1999\u20132005","volume":"8","author":"Hinten","year":"2008","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.mcp.2010.04.003_bib18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.ijms.2004.09.014","article-title":"TIGER: the universal biosensor","volume":"242","author":"Hofstadler","year":"2005","journal-title":"Int J Mass Spectrom"},{"key":"10.1016\/j.mcp.2010.04.003_bib19","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/S0003-2697(03)00024-1","article-title":"A highly efficient and automated method of purifying and desalting PCR products for analysis by electrospray ionization mass spectrometry","volume":"316","author":"Jiang","year":"2003","journal-title":"Anal Biochem"},{"key":"10.1016\/j.mcp.2010.04.003_bib20","doi-asserted-by":"crossref","first-page":"260","DOI":"10.4269\/ajtmh.2002.67.260","article-title":"Growth characteristics of ChimeriVax-DEN2 vaccine virus in Aedes aegypti and Aedes albopictus mosquitoes","volume":"67","author":"Johnson","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/JVI.72.1.73-83.1998","article-title":"Phylogeny of the genus Flavivirus","volume":"72","author":"Kuno","year":"1998","journal-title":"J Virol"},{"key":"10.1016\/j.mcp.2010.04.003_bib22","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/S0065-3527(03)61002-X","article-title":"Molecular amplification assays for the detection of flaviviruses","volume":"61","author":"Lanciotti","year":"2003","journal-title":"Adv Virus Res"},{"key":"10.1016\/j.mcp.2010.04.003_bib23","doi-asserted-by":"crossref","first-page":"4066","DOI":"10.1128\/JCM.38.11.4066-4071.2000","article-title":"Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a Taq Man reverse transcriptase-PCR assay","volume":"38","author":"Lanciotti","year":"2000","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.mcp.2010.04.003_bib24","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1743-422X-5-16","article-title":"Universal primers that amplify RNA from all three flavivirus subgroups","volume":"5","author":"Maher-Sturgess","year":"2008","journal-title":"Virol J"},{"key":"10.1016\/j.mcp.2010.04.003_bib25","doi-asserted-by":"crossref","first-page":"1543","DOI":"10.1021\/ac961134r","article-title":"Length and base composition of PCR-amplified nucleic acids using mass measurements from electrospray ionization mass spectrometry","volume":"69","author":"Muddiman","year":"1997","journal-title":"Anal Chem"},{"key":"10.1016\/j.mcp.2010.04.003_bib26","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0923-2516(07)80011-2","article-title":"Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription\/polymerase chain reaction","volume":"145","author":"Pierre","year":"1994","journal-title":"Res Virol"},{"key":"10.1016\/j.mcp.2010.04.003_bib27","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4269\/ajtmh.2006.74.132","article-title":"Quantitative analysis of dengue-2 virus RNA during the extrinsic incubation period in individual Aedes aegypti","volume":"74","author":"Richardson","year":"2006","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib28","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1196\/annals.1408.008","article-title":"Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry","volume":"1102","author":"Sampath","year":"2007","journal-title":"Ann N Y Acad Sci"},{"key":"10.1016\/j.mcp.2010.04.003_bib29","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jcv.2009.04.001","article-title":"Dengue and Chikungunya virus co-infection in a German traveller","volume":"45","author":"Schilling","year":"2009","journal-title":"J Clin Virol"},{"key":"10.1016\/j.mcp.2010.04.003_bib30","doi-asserted-by":"crossref","first-page":"200","DOI":"10.4269\/ajtmh.2002.66.200","article-title":"Etiology of encephalitis syndrome among hospitalized children and adults in Takeo, Cambodia, 1999\u20132000","volume":"66","author":"Srey","year":"2002","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib31","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1056\/NEJMoa0806326","article-title":"Fatal case of deer tick virus encephalitis","volume":"360","author":"Tavakoli","year":"2009","journal-title":"N Engl J Med"},{"key":"10.1016\/j.mcp.2010.04.003_bib32","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3201\/eid0302.970209","article-title":"A new tick-borne encephalitis-like virus infecting New England deer ticks, Ixodes dammini","volume":"3","author":"Telford","year":"1997","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.mcp.2010.04.003_bib33","article-title":"Assessment of polymicrobial infections in ticks in New York State","author":"Tokarz","year":"2009","journal-title":"Vector Borne Zoonotic Dis"},{"key":"10.1016\/j.mcp.2010.04.003_bib34","doi-asserted-by":"crossref","first-page":"344","DOI":"10.4269\/ajtmh.2008.78.344","article-title":"Assay for and replication of Karshi (mammalian tick-borne flavivirus group) virus in mice","volume":"78","author":"Turell","year":"2008","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2010.04.003_bib35","doi-asserted-by":"crossref","first-page":"605","DOI":"10.2307\/1592901","article-title":"A reverse transcriptase-polymerase chain reaction assay for detecting Highlands J virus","volume":"45","author":"Whitehouse","year":"2001","journal-title":"Avian Dis"},{"key":"10.1016\/j.mcp.2010.04.003_bib36","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0035-9203(97)90215-7","article-title":"Isolation of a flavivirus related to the tick-borne encephalitis complex from human cases in Saudi Arabia","volume":"91","author":"Zaki","year":"1997","journal-title":"Trans R Soc Trop Med Hyg"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089085081000023X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089085081000023X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,6,3]],"date-time":"2020-06-03T23:09:42Z","timestamp":1591225782000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S089085081000023X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S089085081000023X"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2010.04.003","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2010,8]]}}}