{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T22:57:48Z","timestamp":1780441068125,"version":"3.54.1"},"reference-count":37,"publisher":"Elsevier BV","issue":"3-4","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2013,6]]},"DOI":"10.1016\/j.mcp.2013.01.003","type":"journal-article","created":{"date-parts":[[2013,2,9]],"date-time":"2013-02-09T03:54:15Z","timestamp":1360382055000},"page":"122-128","source":"Crossref","is-referenced-by-count":35,"title":["Quantitative real time PCR assays for the detection of Leishmania (Viannia) braziliensis in animals and humans"],"prefix":"10.1016","volume":"27","author":[{"given":"Milena","family":"de Paiva Cavalcanti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u00eania","family":"da Cunha Gon\u00e7alves de Albuquerque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayana Carla","family":"Silva de Morais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Edileuza Felinto","family":"de Brito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Otranto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinval Pinto","family":"Brand\u00e3o-Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2013.01.003_bib1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1038\/nrmicro1748","article-title":"Visceral leishmaniasis: what are the needs for diagnosis, treatment and control?","volume":"5","author":"Chappuis","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.mcp.2013.01.003_bib2","series-title":"Topley & Wilson's microbiology and microbial infections, parasitology","first-page":"313","article-title":"New world leishmaniasis","author":"Lainson","year":"2005"},{"key":"10.1016\/j.mcp.2013.01.003_bib3","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S0037-86822003000100011","article-title":"Leishmaniose tegumentar americana","volume":"36","author":"Gontijo","year":"2003","journal-title":"Rev Soc Bras Med Trop"},{"key":"10.1016\/j.mcp.2013.01.003_bib4","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1186\/1756-3305-2-S1-S1","article-title":"Canine leishmaniasis in South America","volume":"2","author":"Dantas-Torres","year":"2009","journal-title":"Parasite Vector"},{"key":"10.1016\/j.mcp.2013.01.003_bib5","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1590\/S0074-02762009000700001","article-title":"Proven and putative vectors of American cutaneous leishmaniasis in Brazil: aspects of their biology and vectorial competence","volume":"104","author":"Rangel","year":"2009","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.mcp.2013.01.003_bib6","doi-asserted-by":"crossref","first-page":"489","DOI":"10.4269\/ajtmh.1987.36.489","article-title":"Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods","volume":"36","author":"Weigle","year":"1987","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mcp.2013.01.003_bib7","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1128\/CDLI.7.2.318-321.2000","article-title":"Identification of potentially diagnostic Leishmania braziliensis antigens in human cutaneous leishmaniasis by immunoblot analysis","volume":"7","author":"Brito","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.mcp.2013.01.003_bib8","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1473-3099(04)01044-8","article-title":"PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings","volume":"4","author":"Yang","year":"2004","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.mcp.2013.01.003_bib9","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.tvjl.2008.05.018","article-title":"The development of a real-time PCR assay for the quantification of Leishmania infantum DNA in canine blood","volume":"189","author":"Paiva-Cavalcanti","year":"2009","journal-title":"Vet J"},{"key":"10.1016\/j.mcp.2013.01.003_bib10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1590\/S1678-91992010000400004","article-title":"Comparison of real-time PCR and conventional PCR for detection of Leishmania (Leishmania) infantum infection: a mini-review","volume":"16","author":"Paiva-Cavalcanti","year":"2010","journal-title":"J\u00a0Venom Anim Toxin Incl Trop Dis"},{"key":"10.1016\/j.mcp.2013.01.003_bib11","doi-asserted-by":"crossref","first-page":"5249","DOI":"10.1128\/JCM.42.11.5249-5255.2004","article-title":"Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity","volume":"42","author":"Mary","year":"2004","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.mcp.2013.01.003_bib12","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1645\/GE-264R1","article-title":"Quantification of Leishmania infantum parasites in tissue biopsies by real-time polymerase chain reaction and polymerase chain reaction-enzyme linked immunosorbent assay","volume":"90","author":"Rol\u00e3o","year":"2004","journal-title":"J\u00a0Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib13","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1196\/annals.1307.018","article-title":"TaqMan-based detection of Leishmania infantum DNA using canine samples","volume":"1026","author":"Vitale","year":"2004","journal-title":"Acad Sci"},{"key":"10.1016\/j.mcp.2013.01.003_bib14","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2006.01.011","article-title":"Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis","volume":"137","author":"Francino","year":"2006","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib15","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.trstmh.2010.09.003","article-title":"Advantages and limits of real-time PCR assay and PCR-restriction fragment length polymorphism for the identification of cutaneous Leishmania species in Tunisia","volume":"105","author":"Bem Abda","year":"2011","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2013.01.003_bib16","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.pt.2012.08.007","article-title":"Canine leishmaniosis in the old and new worlds: unveiled similarities and differences","volume":"28","author":"Dantas-Torres","year":"2012","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib17","first-page":"115","article-title":"PCR detection of Leishmania in skin biopsies","volume":"3","author":"Lemrani","year":"2009","journal-title":"J\u00a0Infect Dev Ctries"},{"key":"10.1016\/j.mcp.2013.01.003_bib18","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.meegid.2010.11.019","article-title":"PCR-RFLP of ribosomal internal transcribed spacers highlights inter and intra-species variation among Leishmania strains native to La Paz, Bol\u00edvia","volume":"11","author":"Buitrago","year":"2011","journal-title":"Infect Genet Evol"},{"key":"10.1016\/j.mcp.2013.01.003_bib19","doi-asserted-by":"crossref","first-page":"3892","DOI":"10.1128\/JCM.r00764-11","article-title":"Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples","volume":"49","author":"Weirather","year":"2011","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.mcp.2013.01.003_bib20","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1093\/molbev\/msm092","article-title":"MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0","volume":"24","author":"Tamura","year":"2007","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.mcp.2013.01.003_bib21","series-title":"PCR technology, current innovations","first-page":"5","article-title":"The design of primers for PCR","author":"Sharrocks","year":"1994"},{"key":"10.1016\/j.mcp.2013.01.003_bib22","series-title":"Clonagem g\u00eanica e an\u00e1lise de DNA: uma introdu\u00e7\u00e3o","author":"Brown","year":"2003"},{"key":"10.1016\/j.mcp.2013.01.003_bib23","series-title":"Real time PCR systems. Absolute quantification getting started guide","author":"Applied Biosystems","year":"2005"},{"key":"10.1016\/j.mcp.2013.01.003_bib24","series-title":"Control of the leishmaniases","author":"World Health Organization","year":"2010"},{"key":"10.1016\/j.mcp.2013.01.003_bib25","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/S0169-328X(03)00169-4","article-title":"Real time PCR quantification of GFR-2 alternatively spliced isoforms in murine brain and peripheral tissues","volume":"114","author":"Too","year":"2003","journal-title":"Mol Brain Res"},{"key":"10.1016\/j.mcp.2013.01.003_bib26","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0001-706X(92)90006-J","article-title":"Diagnosis of new world leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of kinetoplast DNA","volume":"52","author":"Bruijn","year":"1992","journal-title":"Acta Trop"},{"key":"10.1016\/j.mcp.2013.01.003_bib27","series-title":"BIOESTAT \u2013 Aplica\u00e7\u00f5es estat\u00edsticas nas \u00e1reas das ci\u00eancias bio-m\u00e9dicas","author":"Ayres","year":"2007"},{"key":"10.1016\/j.mcp.2013.01.003_bib28","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0035-9203(02)90052-0","article-title":"Analysis of minicircle classes in Leishmania (Viannia) species","volume":"96","author":"Brewster","year":"2002","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2013.01.003_bib29","doi-asserted-by":"crossref","first-page":"S75","DOI":"10.1016\/S0035-9203(02)90055-6","article-title":"Homologous minicircles in Leishmania donovani","volume":"96","author":"Lambson","year":"2002","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2013.01.003_bib30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(92)90208-2","article-title":"Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites","volume":"51","author":"Van Eys","year":"1992","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib31","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0166-6851(95)00108-D","article-title":"Intergenic region typing (IRT): a rapid molecular approach to the characterization and evolution of Leishmania","volume":"73","author":"Cupolillo","year":"1995","journal-title":"Mol Biochem Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib32","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1111\/j.1365-3156.2009.02361.x","article-title":"Species diversity of Leishmania (Viannia) parasites circulating in an endemic area for cutaneous leishmaniasis located in the Atlantic rainforest region of northeastern Brazil","volume":"14","author":"Brito","year":"2009","journal-title":"Trop Med Int Health"},{"key":"10.1016\/j.mcp.2013.01.003_bib33","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s00436-010-2139-9","article-title":"Characterization of Leishmania spp. causing cutaneous leishmaniasis in Manaus, Amazonas, Brazil","volume":"108","author":"Coelho","year":"2011","journal-title":"Parasitol Res"},{"key":"10.1016\/j.mcp.2013.01.003_bib34","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0014-4894(90)90031-7","article-title":"Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania","volume":"71","author":"Rodgers","year":"1990","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.mcp.2013.01.003_bib35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0035-9203(00)90093-2","article-title":"Genetic heterogeneity of ribosomal internal transcribed spacer (its) in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms (sscp) and sequencing","volume":"94","author":"El Tai","year":"2000","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"10.1016\/j.mcp.2013.01.003_bib36","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S0732-8893(03)00093-2","article-title":"PCR diagnosis and characterization of Leishmania in local and imported clinical samples","volume":"47","author":"Schonian","year":"2003","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.mcp.2013.01.003_bib37","first-page":"419","article-title":"Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material","volume":"70","author":"Meredith","year":"1993","journal-title":"Arch Inst Pasteur Tunis"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850813000108?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850813000108?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T03:21:39Z","timestamp":1595388099000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850813000108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":37,"journal-issue":{"issue":"3-4","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["S0890850813000108"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2013.01.003","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2013,6]]}}}