{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:18:49Z","timestamp":1764332329648},"reference-count":30,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Istituto di Ricerca Virologica Oretta Bartolomei Corsi"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1016\/j.mcp.2015.08.006","type":"journal-article","created":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T16:47:39Z","timestamp":1441039659000},"page":"408-413","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples"],"prefix":"10.1016","volume":"29","author":[{"given":"Rosaria","family":"Arvia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"Corcioli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nunziata","family":"Ciccone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nunzia","family":"Della Malva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberta","family":"Azzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2015.08.006_bib1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1473-3099(01)00170-0","article-title":"Estimates of world wide distribution of child deaths from acute respiratory infections","volume":"2","author":"Williams","year":"2002","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib2","first-page":"325","article-title":"Viral infection in patients with severe pneumonia requiring intensive care unit admission","volume":"186","author":"Choi","year":"2012","journal-title":"AJRCCM"},{"key":"10.1016\/j.mcp.2015.08.006_bib3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S1526-0542(04)90037-1","article-title":"Viral pneumonia in children: incidence and aetiology","volume":"5","author":"Sinaniotis","year":"2004","journal-title":"Paediatr. Respir. Rev."},{"issue":"no. 1","key":"10.1016\/j.mcp.2015.08.006_bib4","article-title":"Prevalence of viral pathogens among pediatric patients admitted for pneumonia in a local tertiary hospital","volume":"13","author":"Perez","year":"2012","journal-title":"PIDSP J."},{"key":"10.1016\/j.mcp.2015.08.006_bib5","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.diagmicrobio.2013.03.008","article-title":"Clinical and economical impact of multiplex respiratory virus assays","volume":"76","author":"Vallieres","year":"2013","journal-title":"Diagn Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib6","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1128\/JCM.02318-09","article-title":"Unique finding of a 2009 H1N1 influenza virus-positive clinical sample suggests matrix gene sequence variation","volume":"48","author":"Zheng","year":"2010","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib7","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.diagmicrobio.2014.01.016","article-title":"Evaluation of the AdvanSure\u2122 real-time RT-PCR compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses","volume":"79","author":"Cho","year":"2014","journal-title":"Diagnostic Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jviromet.2005.01.020","article-title":"Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses","volume":"126","author":"Bellau-Pujol","year":"2005","journal-title":"J.\u00a0Virol. Methods"},{"key":"10.1016\/j.mcp.2015.08.006_bib9","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1128\/CMR.00037-07","article-title":"Detection of respiratory viruses by molecular methods","volume":"21","author":"Mahony","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"issue":"Suppl 4","key":"10.1016\/j.mcp.2015.08.006_bib10","doi-asserted-by":"crossref","first-page":"S326","DOI":"10.1093\/cid\/cir047","article-title":"Multiplex PCR and emerging technologies for the detection of respiratory pathogens","volume":"52","author":"Caliendo","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib11","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/1743-422X-6-89","article-title":"Respiratory viral infections detected by multiplex PCR among pediatric patients with lower respiratory tract infections seen at an urban hospital in Delhi from 2005 to 2007","volume":"6","author":"Bharaj","year":"2009","journal-title":"Virol. J."},{"key":"10.1016\/j.mcp.2015.08.006_bib12","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jcv.2009.12.010","article-title":"Prospective evaluation of a novel multiplex real-time PCR assay for detection of fifteen respiratory pathogens-duration of symptoms significantly affects detection rate","volume":"47","author":"Brittain-Long","year":"2010","journal-title":"J.\u00a0Clin. Virol."},{"key":"10.1016\/j.mcp.2015.08.006_bib13","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1128\/JCM.02436-06","article-title":"Development of a respiratory virus panel test for detection of twenty human respiratory viruses by use of multiplex PCR and a fluid microbead-based assay","volume":"45","author":"Mahony","year":"2007","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib14","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.1128\/JCM.00878-08","article-title":"Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections","volume":"46","author":"Pabbaraju","year":"2008","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib15","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1007\/s00705-013-1876-3","article-title":"CAPNETZ study group. Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009\/10","volume":"159","author":"Bierbaum","year":"2013","journal-title":"Arch. Virol."},{"key":"10.1016\/j.mcp.2015.08.006_bib16","article-title":"Comparison of two multiplex PCR assays for the detection of respiratory viral infections","author":"Kim","year":"2013","journal-title":"Clin. Respir. J."},{"key":"10.1016\/j.mcp.2015.08.006_bib17","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1093\/infdis\/jis538","article-title":"Respiratory viral coinfections identified by a 10-Plex real-time reverse-transcription polymerase chain reaction assay in patients hospitalized with severe acute respiratory illness South Africa, 2009\u20132010","volume":"206","author":"Pretorius","year":"2012","journal-title":"J.\u00a0Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib18","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1002\/jmv.20008","article-title":"Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested-PCR assays","volume":"72","author":"Coiras","year":"2004","journal-title":"J.\u00a0Med. Virol."},{"key":"10.1016\/j.mcp.2015.08.006_bib19","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jviromet.2012.12.017","article-title":"Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR","volume":"189","author":"Choudhary","year":"2013","journal-title":"J.\u00a0Virol. Methods"},{"key":"10.1016\/j.mcp.2015.08.006_bib20","series-title":"510(k) Summary for Centers for Disease Control and Prevention Human Influenza Virus Real-time RT-PCR Detection and Characterization Panel","author":"CDC","year":"2008"},{"key":"10.1016\/j.mcp.2015.08.006_bib21","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1128\/JCM.00216-06","article-title":"Comparison of real-time PCR assays with fluorescent-antibody assays for diagnosis of respiratory virus infections in children","volume":"44","author":"Kuypers","year":"2006","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib22","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1542\/peds.2006-1406","article-title":"Clinical disease in children associated with newly described coronavirus subtypes","volume":"119","author":"Kuypers","year":"2007","journal-title":"Pediatrics"},{"key":"10.1016\/j.mcp.2015.08.006_bib23","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1128\/JCM.42.7.3212-3218.2004","article-title":"Amplicon sequencing and improved detection of human rhinovirus in respiratory samples","volume":"42","author":"Deffernez","year":"2004","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib24","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jcv.2004.03.018","article-title":"Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children","volume":"31","author":"Kuypers","year":"2004","journal-title":"J.\u00a0Clin. Virol."},{"key":"10.1016\/j.mcp.2015.08.006_bib25","doi-asserted-by":"crossref","first-page":"6176","DOI":"10.1128\/JCM.43.12.6176-6182.2005","article-title":"Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region","volume":"43","author":"Casas","year":"2005","journal-title":"J.\u00a0Clin. Microbiol."},{"issue":"4","key":"10.1016\/j.mcp.2015.08.006_bib26","doi-asserted-by":"crossref","first-page":"e18928","DOI":"10.1371\/journal.pone.0018928","article-title":"Viral and atypical bacterial detection in acute respiratory infection in children under five years","volume":"6","author":"Bezerra","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2015.08.006_bib27","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.jcv.2013.04.014","article-title":"Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses","volume":"57","author":"Chi","year":"2013","journal-title":"J.\u00a0Clin. Virol."},{"key":"10.1016\/j.mcp.2015.08.006_bib28","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.diagmicrobio.2014.02.010","article-title":"FilmArray\u00ae respiratory panel performance in respiratory samples from neonatal care units","volume":"79","author":"Piralla","year":"2014","journal-title":"Diagn Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2015.08.006_bib29","doi-asserted-by":"crossref","first-page":"2449","DOI":"10.1128\/JCM.02582-10","article-title":"Comparison of two multiplex methods for detection of respiratory viruses: filmarray RP and xTAG RVP","volume":"49","author":"Rand","year":"2011","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.mcp.2015.08.006_bib30","first-page":"327620","article-title":"A\u00a0Two-Tube Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Sixteen Human Respiratory Virus Types\/Subtypes","volume":"2013","author":"Jin","year":"2013","journal-title":"Biomed. Res. Int."}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850815300323?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850815300323?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,9,19]],"date-time":"2018-09-19T21:21:46Z","timestamp":1537392106000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850815300323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["S0890850815300323"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2015.08.006","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2015,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2015.08.006","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}