{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:17:16Z","timestamp":1790212636993,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Human Ethics Committee of Universiti Sains Malaysia","award":["USM\/JEPeM\/16080260"],"award-info":[{"award-number":["USM\/JEPeM\/16080260"]}]},{"name":"Medical Research and Ethics Committee (MREC) of Ministry of Health Malaysia","award":["NMRR-16-2117-33181"],"award-info":[{"award-number":["NMRR-16-2117-33181"]}]},{"name":"Research University Grant","award":["1001\/PPSP\/812144"],"award-info":[{"award-number":["1001\/PPSP\/812144"]}]},{"DOI":"10.13039\/501100007140","name":"Public Service Department (JPA) Malaysia through the Yang di-Pertuan Agong Scholarship programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007140","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004726","name":"Ministry of Health Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2018,4]]},"DOI":"10.1016\/j.mcp.2018.03.001","type":"journal-article","created":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T02:15:37Z","timestamp":1520475337000},"page":"1-6","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"special_numbering":"C","title":["Development and validation of pan- Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens"],"prefix":"10.1016","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5888-3526","authenticated-orcid":false,"given":"Mohammad Ridhuan","family":"Mohd Ali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira Wahida","family":"Mohd Safee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nor Hayati","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roslinda","family":"Abu Sapian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Mat Hussin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9458-2288","authenticated-orcid":false,"given":"Nabilah","family":"Ismail","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Yean Yean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2018.03.001_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003898","article-title":"Global morbidity and mortality of leptospirosis: a systematic review","volume":"9","author":"Costa","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib2","article-title":"Leptospira venezuelensis sp. nov., a new member of the intermediate group isolated from rodents, cattle and humans","author":"Puche","year":"2017","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib3","first-page":"65","article-title":"Leptospirosis in humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.mcp.2018.03.001_bib4","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.2217\/fmb.10.102","article-title":"Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses","volume":"5","author":"Evangelista","year":"2010","journal-title":"Future Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1017\/S0950268812001951","article-title":"Enzyme-linked immunosorbent assay to diagnose human leptospirosis: a meta-analysis of the published literature","volume":"141","author":"Signorini","year":"2013","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.mcp.2018.03.001_bib6","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1093\/cid\/cis403","article-title":"Fool's gold: why imperfect reference tests are undermining the evaluation of novel diagnostics: a reevaluation of 5 diagnostic tests for leptospirosis","volume":"55","author":"Limmathurotsakul","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib7","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis","volume":"14","author":"Levett","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.mcp.2018.03.001_bib8","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.aca.2015.11.015","article-title":"Development of multiplex loop mediated isothermal amplification (m-LAMP) label-based gold nanoparticles lateral flow dipstick biosensor for detection of pathogenic Leptospira","volume":"903","author":"Nurul Najian","year":"2016","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.mcp.2018.03.001_bib9","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.diagmicrobio.2006.07.014","article-title":"Detection and differentiation between pathogenic and saprophytic Leptospira spp. by multiplex polymerase chain reaction","volume":"57","author":"Kositanont","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib10","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.mimet.2012.06.005","article-title":"Comparison of conventional PCR, quantitative PCR, bacteriological culture and the Warthin Starry technique to detect Leptospira spp. in kidney and liver samples from naturally infected sheep from Brazil","volume":"90","author":"Fornazari","year":"2012","journal-title":"J. Microbiol. Meth."},{"key":"10.1016\/j.mcp.2018.03.001_bib11","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.diagmicrobio.2009.03.014","article-title":"Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene","volume":"64","author":"Stoddard","year":"2009","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib12","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.1128\/JCM.02452-10","article-title":"Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences","volume":"49","author":"Bourhy","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/annotation\/e2b77844-576a-4d20-b698-09ad057872fc","article-title":"Diagnostic accuracy of real-time PCR assays targeting 16S rRNA and lipL32 genes for human leptospirosis in Thailand: a case-control study","volume":"6","author":"Thaipadungpanit","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2018.03.001_bib14","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.meegid.2015.11.033","article-title":"Isolation and clinical sample typing of human leptospirosis cases in Argentina","volume":"37","author":"Chiani","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.mcp.2018.03.001_bib15","doi-asserted-by":"crossref","first-page":"865","DOI":"10.3201\/eid0808.010445","article-title":"Human infection caused by Leptospira fainei","volume":"8","author":"Arzouni","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib16","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1099\/0022-1317-50-1-96","article-title":"First isolation of Leptospira fainei serovar Hurstbridge from two human patients with Weil's syndrome","volume":"50","author":"Krogfelt","year":"2001","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002114","article-title":"Serovar diversity of pathogenic Leptospira circulating in the French West Indies","volume":"7","author":"Bourhy","year":"2013","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib18","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1590\/S0100-879X2004000400003","article-title":"Genome features of Leptospira interrogans serovar Copenhageni","volume":"37","author":"Nascimento","year":"2004","journal-title":"Braz. J. Med. Biol. Res. Rev. Bras. Pesqui. Med. E Biol."},{"key":"10.1016\/j.mcp.2018.03.001_bib19","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0007093","article-title":"Development and validation of a real-time PCR for detection of pathogenic Leptospira species in clinical materials","volume":"4","author":"Ahmed","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2018.03.001_bib20","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.jmii.2013.03.001","article-title":"Laboratory diagnosis of leptospirosis: a challenge","volume":"46","author":"Mussoa","year":"2013","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.mcp.2018.03.001_bib21","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mcp.2018.03.001_bib22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1099\/jmm.0.45860-0","article-title":"Detection of pathogenic leptospires by real-time quantitative PCR","volume":"54","author":"Levett","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.femsle.2005.06.011","article-title":"A rapid and quantitative method for the detection of Leptospira species in human leptospirosis","volume":"249","author":"Merien","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.mcp.2018.03.001_bib24","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.diagmicrobio.2006.10.004","article-title":"Evaluation of a modified Taqman assay detecting pathogenic Leptospira spp. against culture and Leptospira-specific IgM enzyme-linked immunosorbent assay in a clinical environment","volume":"57","author":"Slack","year":"2007","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.03.001_bib25","doi-asserted-by":"crossref","first-page":"167","DOI":"10.33073\/pjm-2010-026","article-title":"Simultaneous detection and differentiation of pathogenic and nonpathogenic Leptospira spp. by multiplex real-time PCR (TaqMan) assay","volume":"59","author":"Bedir","year":"2010","journal-title":"Pol. J. Microbiol."},{"key":"10.1016\/j.mcp.2018.03.001_bib26","doi-asserted-by":"crossref","first-page":"4953","DOI":"10.3390\/ijerph110504953","article-title":"Development of a recombinase polymerase amplification assay for the detection of pathogenic Leptospira","volume":"11","author":"Ahmed","year":"2014","journal-title":"Int. J. Environ. Res. Publ. Health"},{"key":"10.1016\/j.mcp.2018.03.001_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0001607","article-title":"Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis","volume":"3","author":"Picardeau","year":"2008","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2018.03.001_bib28","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1111\/j.1348-0421.2003.tb03400.x","article-title":"Probable laboratory contamination of clinical specimens with Leptospira meyeri","volume":"47","author":"Koizumi","year":"2003","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.mcp.2018.03.001_bib29","doi-asserted-by":"crossref","first-page":"5763","DOI":"10.1128\/jb.171.11.5763-5767.1989","article-title":"Unique organization of Leptospira interrogans rRNA genes","volume":"171","author":"Fukunaga","year":"1989","journal-title":"J. Bacteriol."}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850817301275?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850817301275?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T23:38:46Z","timestamp":1603928326000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850817301275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":29,"alternative-id":["S0890850817301275"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2018.03.001","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2018,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and validation of pan-Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2018.03.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}