{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T20:14:22Z","timestamp":1747167262336,"version":"3.40.5"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,8,1]],"date-time":"2018-08-01T00:00:00Z","timestamp":1533081600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Verona University Dipartimental Research Funds"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1016\/j.mcp.2018.06.001","type":"journal-article","created":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T08:07:05Z","timestamp":1528186025000},"page":"13-18","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples"],"prefix":"10.1016","volume":"40","author":[{"given":"Marco","family":"Ligozzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana","family":"Galia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Carelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9432-0595","authenticated-orcid":false,"given":"Pier Paolo","family":"Piccaluga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Diani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Gibellini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2018.06.001_bib1","doi-asserted-by":"crossref","first-page":"4130","DOI":"10.1128\/JVI.00028-07","article-title":"Identification of a third human polyomavirus","volume":"81","author":"Allander","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1371\/journal.ppat.0030064","article-title":"Identification of a novel polyomavirus from patients with acute respiratory tract infections","volume":"3","author":"Gaynor","year":"2007","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.mcp.2018.06.001_bib3","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1002\/jmv.21559","article-title":"Excretion of the novel polyomaviruses KI and Wu in the stool of patients with hematological disorders","volume":"81","author":"Babakir-Mina","year":"2009","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib4","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1086\/648095","article-title":"Wu and KI polyomaviruses in the brains of HIV-positive patients with and without progressive multifocal leukoencephalopathy","volume":"200","author":"Barzon","year":"2009","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib5","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1002\/jmv.23602","article-title":"Molecular epidemiology of KI and Wu polyomaviruses in healthy blood donors, south-eastern France","volume":"85","author":"Touinssi","year":"2013","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib6","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.jcv.2014.12.020","article-title":"Wu and KI polyomaviruses in respiratory, blood and urine samples from renal transplant patients","volume":"64","author":"Csoma","year":"2015","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib7","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.3201\/eid1508.090270","article-title":"Serological evidence of frequent human infection by the Wu and KI polyomaviruses","volume":"15","author":"Nguyen","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib8","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/1471-2334-10-215","article-title":"High prevalence of antibodies against polyomavirus Wu, polyomavirus KI, and human bocavirus in German blood donors","volume":"10","author":"Neske","year":"2010","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib9","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1089\/vim.2009.0083","article-title":"Expression and serological characterization of polyomavirus WUPyV and KIPyV structural proteins","volume":"23","author":"Kantola","year":"2010","journal-title":"Viral Immunol."},{"key":"10.1016\/j.mcp.2018.06.001_bib10","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.virol.2012.12.015","article-title":"Human polyomaviruses in disease and cancer","volume":"437","author":"Dalianis","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.mcp.2018.06.001_bib11","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.jcv.2008.08.003","article-title":"Wu and KI polyomavirus present in the respiratory tract of children, but not in immunocompetent adults","volume":"43","author":"Ren","year":"2008","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib12","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3201\/eid1501.080758","article-title":"Polyomaviruses KI and Wu in immunocompromised patients with respiratory disease","volume":"15","author":"Mourez","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib13","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1086\/596062","article-title":"Reactivation and mutation of newly discovered Wu, KI, and Merkel cell carcinoma polyomaviruses in immunosuppressed individuals","volume":"199","author":"Sharp","year":"2009","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib14","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.semcancer.2009.04.001","article-title":"KI, Wu and Merkel cell polyomaviruses: a new era for human polyomavirus research","volume":"19","author":"Dalianis","year":"2009","journal-title":"Semin. Canc. Biol."},{"key":"10.1016\/j.mcp.2018.06.001_bib15","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.jcv.2009.05.002","article-title":"Detection of BK, JC, Wu, or KI polyomaviruses in faecal, urine, blood, cerebrospinal fluid and respiratory samples","volume":"45","author":"Bialasiewicz","year":"2009","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib16","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.3201\/eid1411.080464","article-title":"Prevalence and pathogenicity of Wu and KI polyomaviruses in children, The Netherlands","volume":"14","author":"Van der Zalm","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib17","doi-asserted-by":"crossref","first-page":"1440","DOI":"10.1002\/jmv.22123","article-title":"Wu polyomavirus infection among children in South China","volume":"83","author":"Zhuang","year":"2009","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib18","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1007\/s00705-009-0498-2","article-title":"Polyomaviruses KI and Wu in children with respiratory tract infection","volume":"154","author":"Mueller","year":"2009","journal-title":"Arch. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib19","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s10529-011-0832-0","article-title":"Patch oligodeoxynucleotide synthesis (POS): a novel method for synthesis of long DNA sequences and full-length genes","volume":"34","author":"Yang","year":"2012","journal-title":"Biotechnol. Lett."},{"key":"10.1016\/j.mcp.2018.06.001_bib20","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1126\/science.79.2037.38","article-title":"The method of probit","volume":"79","author":"Bliss","year":"1934","journal-title":"Science"},{"key":"10.1016\/j.mcp.2018.06.001_bib21","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.mcp.2005.12.005","article-title":"Simultaneous detection of HCV and HIV-1 by SYBR Green real-time multiplex RT-PCR technique in plasma samples","volume":"20","author":"Gibellini","year":"2006","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mcp.2018.06.001_bib22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jviromet.2009.12.017","article-title":"HIV-1 and HCV detection in dried blood spots by SYBR Green multiplex real-time RT-PCR","volume":"165","author":"De Crignis","year":"2010","journal-title":"J. Virol Meth."},{"key":"10.1016\/j.mcp.2018.06.001_bib23","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s12985-015-0255-y","article-title":"A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae","volume":"12","author":"Huang","year":"2015","journal-title":"Virol. J."},{"key":"10.1016\/j.mcp.2018.06.001_bib24","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jcv.2007.07.015","article-title":"Development and evaluation of real-time PCR assays for the detection of the newly identified KI and Wu polyomaviruses","volume":"40","author":"Bialasiewicz","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib25","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jviromet.2008.06.018","article-title":"Detection and discrimination of Wu\/KI polyomaviruses by real-time PCR with melting curve analysis","volume":"153","author":"Payungporn","year":"2008","journal-title":"J. Virol Meth."},{"key":"10.1016\/j.mcp.2018.06.001_bib26","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jcv.2008.09.006","article-title":"A single-tube, real-time PCR assay for detection of the two newly characterized human KI and Wu polyomaviruses","volume":"44","author":"Lindau","year":"2009","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib27","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.3201\/eid1312.070909","article-title":"Wu polyomavirus in children, Canada","volume":"13","author":"Abed","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib28","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.3201\/eid1311.070872","article-title":"Wu polyomavirus in children with acute lower respiratory tract infections, South Korea","volume":"13","author":"Han","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib29","doi-asserted-by":"crossref","first-page":"680","DOI":"10.3201\/eid1404.071325","article-title":"Wu polyomavirus infection in children, Germany","volume":"14","author":"Neske","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib30","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.virusres.2008.03.018","article-title":"Prevalence and molecular characterization of Wu\/KI polyomaviruses isolated from pediatric patients with respiratory disease in Thailand","volume":"135","author":"Payungporn","year":"2008","journal-title":"Virus Res."},{"key":"10.1016\/j.mcp.2018.06.001_bib31","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1111\/j.1348-0421.2011.00346.x","article-title":"Detection of KI polyomavirus and Wu polyomavirus DNA by real-time polymerase chain reaction in nasopharyngeal swabs and in normal lung and lung adenocarcinoma tissues","volume":"55","author":"Teramoto","year":"2011","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.mcp.2018.06.001_bib32","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/apm.12091","article-title":"The human polyomaviruses KI and Wu: virological background and clinical implications","volume":"121","author":"Babakir-Mina","year":"2013","journal-title":"APMIS"},{"key":"10.1016\/j.mcp.2018.06.001_bib33","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.3201\/eid1312.070977","article-title":"Clinical and epidemiologic characterization of Wu polyomavirus infection, St. Louis, Missouri","volume":"13","author":"Lee","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib34","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jcv.2008.05.003","article-title":"Age-related pattern of KI and Wu polyomavirus infection","volume":"43","author":"Abedi Kiasari","year":"2008","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib35","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.3201\/eid1411.080394","article-title":"Role of human polyomaviruses in respiratory tract disease in young children","volume":"14","author":"Wattier","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.mcp.2018.06.001_bib36","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jcv.2007.11.001","article-title":"Presence of the newly discovered human polyomaviruses KI and Wu in Australian patients with acute respiratory tract infection","volume":"41","author":"Bialasiewicz","year":"2008","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2018.06.001_bib37","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jcv.2016.07.013","article-title":"Wu and KI polyomavirus infections in Filipino children with lower respiratory tract disease","volume":"82","author":"Rao","year":"2016","journal-title":"J. Clin. Virol."}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850818301269?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850818301269?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,7,30]],"date-time":"2018-07-30T02:50:30Z","timestamp":1532919030000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850818301269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8]]},"references-count":37,"alternative-id":["S0890850818301269"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2018.06.001","relation":{},"ISSN":["0890-8508"],"issn-type":[{"type":"print","value":"0890-8508"}],"subject":[],"published":{"date-parts":[[2018,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2018.06.001","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}