{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:42:23Z","timestamp":1760193743203,"version":"build-2065373602"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100010623","name":"University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010623","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2019,6]]},"DOI":"10.1016\/j.mcp.2019.03.003","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T18:39:27Z","timestamp":1553020767000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["\u0391 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA"],"prefix":"10.1016","volume":"45","author":[{"given":"M.","family":"Daskou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tsakogiannis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.G.","family":"Dimitriou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Manali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Apti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.D.","family":"Amoutzias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Mossialos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0979-3871","authenticated-orcid":false,"given":"C.","family":"Kottaridi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Markoulatos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2019.03.003_bib1","first-page":"F55","article-title":"Papillomavirus infections - a major cause of human cancers","volume":"1288","author":"zur Hausen","year":"1996","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.mcp.2019.03.003_bib2","doi-asserted-by":"crossref","first-page":"F55","DOI":"10.1016\/j.vaccine.2012.06.083","article-title":"The biology and life-cycle of human papillomaviruses","volume":"30","author":"Doorbar","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.mcp.2019.03.003_bib3","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1002\/cncr.30588","article-title":"Human papillomavirus in cervical cancer and oropharyngeal cancer: one cause, two diseases","volume":"123","author":"Berman","year":"2017","journal-title":"Cancer"},{"key":"10.1016\/j.mcp.2019.03.003_bib4","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-981-10-5765-6_3","article-title":"Human papillomavirus-related cancers","volume":"1018","author":"Li","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.mcp.2019.03.003_bib5","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1097\/LGT.0b013e31824ca9d5","article-title":"American cancer society, American society for colposcopy and cervical Pathology, and American society for clinical Pathology screening guidelines for the prevention and early detection of cervical cancer","volume":"16","author":"Saslow","year":"2012","journal-title":"J. Low. Genit. Tract Dis."},{"key":"10.1016\/j.mcp.2019.03.003_bib6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/dc.23874","article-title":"Negative Pap tests in women with high-grade cervical lesions on follow-up biopsies: contributing factors and role of human papillomavirus genotyping","volume":"46","author":"Good man","year":"2018","journal-title":"Diagn. Cytopathol."},{"key":"10.1016\/j.mcp.2019.03.003_bib7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1159\/000484036","article-title":"Comparison of visual inspection with acetic acid and the pap smear for cervical cancer screening","volume":"62","author":"Sinha","year":"2018","journal-title":"Acta Cytol."},{"key":"10.1016\/j.mcp.2019.03.003_bib8","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1017\/erm.2017.2","article-title":"Molecular approaches for HPV genotyping and HPV-DNA physical status","volume":"19","author":"Tsakogiannis","year":"2017","journal-title":"Expert Rev. Mol. Med."},{"key":"10.1016\/j.mcp.2019.03.003_bib9","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/S1470-2045(11)70188-7","article-title":"Performance of carcinogenic human papillomavirus (HPV) testing and HPV16 or HPV18 genotyping for cervical cancer screening of women aged 25 years and older: a subanalysis of the ATHENA study","volume":"12","author":"Castle","year":"2011","journal-title":"Lancet Oncol."},{"key":"10.1016\/j.mcp.2019.03.003_bib10","doi-asserted-by":"crossref","first-page":"184.e1","DOI":"10.1016\/j.ajog.2012.11.020","article-title":"Comparison of cervical cancer screening strategies incorporating different combinations of cytology, HPV testing, and genotyping for HPV 16\/18: results from the ATHENA HPV study","volume":"208","author":"Cox","year":"2013","journal-title":"Am. J. Obstet. Gynecol."},{"key":"10.1016\/j.mcp.2019.03.003_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/LGT.0000000000000170","article-title":"A suggested approach to simplify and improve cervical screening in the United States","volume":"20","author":"Schiffman","year":"2016","journal-title":"J. Low. Genit. Tract Dis."},{"key":"10.1016\/j.mcp.2019.03.003_bib12","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jviromet.2017.07.009","article-title":"Comparison of the analytical and clinical performance of five tests for the detection of human papillomavirus genital infection","volume":"248","author":"Del Pino","year":"2017","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.003_bib13","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1080\/14737159.2017.1293525","article-title":"Molecular tests potentially improving HPV screening and genotyping for cervical cancer prevention","volume":"17","author":"Grad\u00edssimo","year":"2017","journal-title":"Expert Rev. Mol. Diagn."},{"key":"10.1016\/j.mcp.2019.03.003_bib14","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcp.2019.03.003_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12275-015-4656-9","article-title":"Loop-mediated isothermal amplification(LAMP): principle, features, and future prospects","volume":"53","author":"Notomi","year":"2015","journal-title":"J. Microbiol."},{"key":"10.1016\/j.mcp.2019.03.003_bib16","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.jviromet.2016.04.008","article-title":"High sensitivity, loop-mediated isothermal amplification combined with colorimetric gold-nanoparticle probes for visual detection of high risk human papillomavirus genotypes 16 and 18","volume":"234","author":"Kumvongpin","year":"2016","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.003_bib17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jcv.2016.01.002","article-title":"Loop mediated isothermal amplification (LAMP) for the detection and subtyping of human papillomaviruses (HPV) in oropharyngeal squamous cell carcinoma (OPSCC)","volume":"75","author":"Livingstone","year":"2016","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.mcp.2019.03.003_bib18","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1006\/mcpr.2002.0415","article-title":"Accelerated reaction by loop-mediated isothermal amplification using loop primers","volume":"16","author":"Nagamine","year":"2002","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mcp.2019.03.003_bib19","doi-asserted-by":"crossref","first-page":"189","DOI":"10.2144\/000114275","article-title":"Shining a light on LAMP assays--a comparison of LAMP visualization methods including the novel use of berberine","volume":"58","author":"Fischbach","year":"2015","journal-title":"Biotechniques"},{"key":"10.1016\/j.mcp.2019.03.003_bib20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2144\/000114253","article-title":"Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes","volume":"58","author":"Tanner","year":"2015","journal-title":"Biotechniques"},{"key":"10.1016\/j.mcp.2019.03.003_bib21","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jviromet.2007.08.023","article-title":"A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus","volume":"147","author":"Blomstr\u00f6m","year":"2008","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.003_bib22","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1373\/clinchem.2012.193664","article-title":"IsoPCR: an analytically sensitive,nested, multiplex nucleic acid amplification method","volume":"59","author":"S\u00f8e","year":"2013","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mcp.2019.03.003_bib23","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0166-0934(94)00173-E","article-title":"New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay","volume":"53","author":"Casas","year":"1995","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.003_bib24","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1002\/jcla.10082","article-title":"Multiplex PCR: rapid DNA cycling in a conventional thermal cycler","volume":"17","author":"Markoulatos","year":"2003","journal-title":"J. Clin. Lab. Anal."},{"key":"10.1016\/j.mcp.2019.03.003_bib25","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00705-014-2261-6","article-title":"Multiplex PCR assay for the rapid identification of human papillomavirus genotypes 16, 18, 45, 35, 66, 33, 51, 58, and 31 in clinical samples","volume":"160","author":"Tsakogiannis","year":"2015","journal-title":"Arch. Virol."},{"key":"10.1016\/j.mcp.2019.03.003_bib26","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1373\/clinchem.2016.263665","article-title":"Two-stage isothermal enzymatic amplification for concurrent multiplex molecular detection","volume":"63","author":"Song","year":"2017","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mcp.2019.03.003_bib27","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.42.7.3176-3184.2004","article-title":"Detection and typing of human papillomavirus by e6 nested multiplex PCR","volume":"42","author":"Sotlar","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mcp.2019.03.003_bib28","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/JCM.00793-07","article-title":"Genotyping of human papillomaviruses by a novel one-step typing method with multiplex PCR and clinical applications","volume":"46","author":"Nishiwaki","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mcp.2019.03.003_bib29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jviromet.2013.10.024","article-title":"Oncogenic human papillomavirus genotyping by multiplex PCR and fragment analysis","volume":"196","author":"Souho","year":"2014","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.003_bib30","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.mcp.2014.09.003","article-title":"Duplex Real-time PCR assay and SYBR green I melting curve analysis for molecular identification of HPV genotypes 16, 18, 31, 35, 51 and 66","volume":"29","author":"Tsakogiannis","year":"2015","journal-title":"Mol. Cell. Probes"},{"key":"10.1016\/j.mcp.2019.03.003_bib31","doi-asserted-by":"crossref","first-page":"75","DOI":"10.2144\/05391RV01","article-title":"Real-time PCR for mRNA quantitation","volume":"39","author":"Wong","year":"2005","journal-title":"Biotechniques"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850818303074?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850818303074?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:14:36Z","timestamp":1760192076000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850818303074"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":31,"alternative-id":["S0890850818303074"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2019.03.003","relation":{},"ISSN":["0890-8508"],"issn-type":[{"type":"print","value":"0890-8508"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"\u0391 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2019.03.003","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}