{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:27:47Z","timestamp":1780493267059,"version":"3.54.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2019,6]]},"DOI":"10.1016\/j.mcp.2019.03.005","type":"journal-article","created":{"date-parts":[[2019,3,25]],"date-time":"2019-03-25T20:42:27Z","timestamp":1553546547000},"page":"8-13","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"special_numbering":"C","title":["Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of human respiratory syncytial virus"],"prefix":"10.1016","volume":"45","author":[{"given":"Yun","family":"Xi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Zhi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Zhi","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Lin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie-Ming","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.mcp.2019.03.005_bib1","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/infdis\/161.4.640","article-title":"Association between respiratory syncytial virus outbreaks and lower respiratory tract deaths of infants and young children","volume":"161","author":"Anderson","year":"1990","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1086\/314567","article-title":"Respiratory syncytial virus pneumonia among the elderly: an assessment of disease burden","volume":"179","author":"Han","year":"1999","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib3","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1017\/S0950268800058957","article-title":"Impact of influenza and respiratory syncytial virus on mortality in England and Wales from January 1975 to December 1990","volume":"116","author":"Nicholson","year":"1996","journal-title":"Epidemiol. Infect."},{"issue":"9291","key":"10.1016\/j.mcp.2019.03.005_bib4","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1016\/S0140-6736(01)06528-X","article-title":"Contribution of influenza and respiratory syncytial virus to community cases of influenza-like illness: an observational study","volume":"358","author":"Zambon","year":"2001","journal-title":"Lancet (London, England)"},{"issue":"3","key":"10.1016\/j.mcp.2019.03.005_bib5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s12016-013-8368-9","article-title":"Respiratory syncytial virus--a comprehensive review","volume":"45","author":"Borchers","year":"2013","journal-title":"Clin. Rev. Allergy Immunol."},{"issue":"5","key":"10.1016\/j.mcp.2019.03.005_bib6","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1128\/JCM.00008-08","article-title":"Prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections","volume":"46","author":"Aslanzadeh","year":"2008","journal-title":"J. Clin. Microbiol."},{"issue":"8","key":"10.1016\/j.mcp.2019.03.005_bib7","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1080\/17476348.2017.1338567","article-title":"Respiratory syncytial virus: prospects for new and emerging therapeutics","volume":"11","author":"Jorquera","year":"2017","journal-title":"Expert Rev. Respir. Med."},{"issue":"6","key":"10.1016\/j.mcp.2019.03.005_bib8","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1128\/jcm.24.6.1013-1016.1986","article-title":"Comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques","volume":"24","author":"Freymuth","year":"1986","journal-title":"J. Clin. Microbiol."},{"issue":"5","key":"10.1016\/j.mcp.2019.03.005_bib9","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1128\/jcm.25.5.763-767.1987","article-title":"Comparison of nasopharyngeal aspirate and nasopharyngeal swab specimens for respiratory syncytial virus diagnosis by cell culture, indirect immunofluorescence assay, and enzyme-linked immunosorbent assay","volume":"25","author":"Ahluwalia","year":"1987","journal-title":"J. Clin. Microbiol."},{"issue":"3","key":"10.1016\/j.mcp.2019.03.005_bib10","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1128\/jcm.29.3.470-474.1991","article-title":"Comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions","volume":"29","author":"Takimoto","year":"1991","journal-title":"J. Clin. Microbiol."},{"issue":"12","key":"10.1016\/j.mcp.2019.03.005_bib11","doi-asserted-by":"crossref","first-page":"3352","DOI":"10.1128\/jcm.33.12.3352-3355.1995","article-title":"Detection of respiratory syncytial virus by reverse transcription-PCR and hybridization with a DNA enzyme immunoassay","volume":"33","author":"Freymuth","year":"1995","journal-title":"J. Clin. Microbiol."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib12","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/jmv.22186","article-title":"Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections","volume":"84","author":"Sanghavi","year":"2012","journal-title":"J. Med. Virol."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib13","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1186\/s12887-017-0843-7","article-title":"Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms","volume":"17","author":"You","year":"2017","journal-title":"BMC Pediatr."},{"key":"10.1016\/j.mcp.2019.03.005_bib14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jviromet.2017.01.006","article-title":"Reverse-transcription loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus directly from nasopharyngeal swabs","volume":"242","author":"Hoos","year":"2017","journal-title":"J. Virol. Methods"},{"issue":"8","key":"10.1016\/j.mcp.2019.03.005_bib15","doi-asserted-by":"crossref","first-page":"2696","DOI":"10.1128\/JCM.00662-13","article-title":"Development of a sensitive loop-mediated isothermal amplification assay that provides specimen-to-result diagnosis of respiratory syncytial virus infection in 30 minutes","volume":"51","author":"Mahony","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.mcp.2019.03.005_bib16","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jviromet.2014.06.005","article-title":"New method for the visual detection of human respiratory syncytial virus using reverse transcription loop-mediated amplification","volume":"206","author":"Mu","year":"2014","journal-title":"J. Virol. Methods"},{"issue":"7","key":"10.1016\/j.mcp.2019.03.005_bib17","doi-asserted-by":"crossref","first-page":"e204","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biol."},{"issue":"2","key":"10.1016\/j.mcp.2019.03.005_bib18","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.mcp.2016.01.009","article-title":"Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care","volume":"30","author":"Lillis","year":"2016","journal-title":"Mol. Cell. Probes"},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib19","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s12985-017-0792-7","article-title":"Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus","volume":"14","author":"Yang","year":"2017","journal-title":"Virol. J."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib20","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12985-017-0688-6","article-title":"Development of real-time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus","volume":"14","author":"Yang","year":"2017","journal-title":"Virol. J."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib21","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1186\/s12917-018-1736-1","article-title":"Development and evaluation of a broadly reactive reverse transcription recombinase polymerase amplification assay for rapid detection of murine norovirus","volume":"14","author":"Ma","year":"2018","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.mcp.2019.03.005_bib22","doi-asserted-by":"crossref","DOI":"10.1111\/tbed.13155","article-title":"Point-of-care diagnostic assay for rapid detection of porcine deltacoronavirus using the recombinase polymerase amplification method","author":"Ma","year":"2019","journal-title":"Transboundary Emerg. Dis."},{"key":"10.1016\/j.mcp.2019.03.005_bib23","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.mcp.2018.08.006","article-title":"Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus","volume":"41","author":"Ma","year":"2018","journal-title":"Mol. Cell. Probes"},{"issue":"6","key":"10.1016\/j.mcp.2019.03.005_bib24","doi-asserted-by":"crossref","first-page":"e0129682","DOI":"10.1371\/journal.pone.0129682","article-title":"Recombinase polymerase amplification assay for rapid diagnostics of dengue infection","volume":"10","author":"Abd El Wahed","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2019.03.005_bib25","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1186\/s12985-015-0440-z","article-title":"Development of a fluorescent probe-based recombinase polymerase amplification assay for rapid detection of Orf virus","volume":"12","author":"Yang","year":"2015","journal-title":"Virol. J."},{"issue":"2","key":"10.1016\/j.mcp.2019.03.005_bib26","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.diagmicrobio.2017.03.010","article-title":"Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections","volume":"88","author":"Zhao","year":"2017","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib27","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s12985-018-1061-0","article-title":"A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus","volume":"15","author":"Feng","year":"2018","journal-title":"Virol. J."},{"issue":"3","key":"10.1016\/j.mcp.2019.03.005_bib28","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/irv.12518","article-title":"Respiratory syncytial virus evaluation among asymptomatic and symptomatic subjects in a university hospital in Sao Paulo, Brazil, in the period of 2009-2013","volume":"12","author":"Moreira","year":"2018","journal-title":"Influenza Other Respir. Viruses"},{"key":"10.1016\/j.mcp.2019.03.005_bib29","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jviromet.2015.07.011","article-title":"Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection","volume":"223","author":"Yehia","year":"2015","journal-title":"J. Virol. Methods"},{"issue":"9","key":"10.1016\/j.mcp.2019.03.005_bib30","doi-asserted-by":"crossref","first-page":"e0162411","DOI":"10.1371\/journal.pone.0162411","article-title":"A GeXP-based assay for simultaneous detection of multiple viruses in hospitalized children with community acquired pneumonia","volume":"11","author":"Wang","year":"2016","journal-title":"PLoS One"},{"issue":"8","key":"10.1016\/j.mcp.2019.03.005_bib31","doi-asserted-by":"crossref","first-page":"2824","DOI":"10.1128\/JCM.38.8.2824-2828.2000","article-title":"Clinical and financial benefits of rapid detection of respiratory viruses: an outcomes study","volume":"38","author":"Barenfanger","year":"2000","journal-title":"J. Clin. Microbiol."},{"issue":"2","key":"10.1016\/j.mcp.2019.03.005_bib32","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0196-6553(99)70087-8","article-title":"Reducing the rate of nosocomially transmitted respiratory syncytial virus","volume":"27","author":"Karanfil","year":"1999","journal-title":"Am. J. Infect. Contr."},{"issue":"6","key":"10.1016\/j.mcp.2019.03.005_bib33","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1128\/jcm.35.6.1579-1581.1997","article-title":"Cost-effectiveness of rapid diagnosis of viral respiratory tract infections in pediatric patients","volume":"35","author":"Woo","year":"1997","journal-title":"J. Clin. Microbiol."},{"issue":"11","key":"10.1016\/j.mcp.2019.03.005_bib34","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1136\/jcp.2009.066688","article-title":"Clinical evaluation of NucliSENS magnetic extraction and NucliSENS analytical specific reagents for the real-time detection of respiratory syncytial virus (RSV) in paediatric respiratory specimens","volume":"62","author":"Manji","year":"2009","journal-title":"J. Clin. Pathol."},{"issue":"12","key":"10.1016\/j.mcp.2019.03.005_bib35","doi-asserted-by":"crossref","first-page":"e1357","DOI":"10.1371\/journal.pone.0001357","article-title":"Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen","volume":"2","author":"Tillmann","year":"2007","journal-title":"PLoS One"},{"issue":"1\u20132","key":"10.1016\/j.mcp.2019.03.005_bib36","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jviromet.2007.05.030","article-title":"Rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology","volume":"146","author":"Deiman","year":"2007","journal-title":"J. Virol. Methods"},{"issue":"5","key":"10.1016\/j.mcp.2019.03.005_bib37","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1007\/s00705-017-3227-2","article-title":"Clinical evaluation of the isothermal amplification assays for the detection of four common respiratory viruses in children with pneumonia","volume":"162","author":"Zhou","year":"2017","journal-title":"Arch. Virol."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib38","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.jviromet.2006.09.014","article-title":"Diagnosis of human respiratory syncytial virus infection using reverse transcription loop-mediated isothermal amplification","volume":"139","author":"Shirato","year":"2007","journal-title":"J. Virol. Methods"},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib39","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1002\/jmv.20424","article-title":"Detection of respiratory syncytial virus genome by subgroups-A, B specific reverse transcription loop-mediated isothermal amplification (RT-LAMP)","volume":"77","author":"Ushio","year":"2005","journal-title":"J. Med. Virol."},{"issue":"2","key":"10.1016\/j.mcp.2019.03.005_bib40","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.diagmicrobio.2017.10.005","article-title":"Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection","volume":"90","author":"Chen","year":"2018","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"2","key":"10.1016\/j.mcp.2019.03.005_bib41","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00135-13","article-title":"Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification","volume":"4","author":"Boyle","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.mcp.2019.03.005_bib42","article-title":"Epidemiology, risk factors, and outcomes of respiratory syncytial virus infections in newborns in Bamako, Mali","author":"Buchwald","year":"2019","journal-title":"Clin. Infect. Dis."},{"issue":"1","key":"10.1016\/j.mcp.2019.03.005_bib43","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.jmoldx.2013.08.003","article-title":"Sensitive and rapid detection of Chlamydia trachomatis by recombinase polymerase amplification directly from urine samples","volume":"16","author":"Krolov","year":"2014","journal-title":"J. Mol. Diagn. : J. Mod. Dyn."},{"key":"10.1016\/j.mcp.2019.03.005_bib44","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.jviromet.2018.07.011","article-title":"Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick","volume":"261","author":"Wang","year":"2018","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mcp.2019.03.005_bib45","series-title":"Development of a Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral-Flow Dipstick Assay for Avian Influenza H9N2 HA Gene Detection, Transboundary and Emerging Diseases","author":"Wang","year":"2018"}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089085081930009X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S089085081930009X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:14:31Z","timestamp":1760192071000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S089085081930009X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6]]},"references-count":45,"alternative-id":["S089085081930009X"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2019.03.005","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2019,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of human respiratory syncytial virus","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2019.03.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}