{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:48:15Z","timestamp":1740106095031,"version":"3.37.3"},"reference-count":46,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103434"],"award-info":[{"award-number":["P20GM103434"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000104","name":"National Aeronautics and Space Administration","doi-asserted-by":"publisher","award":["NNX15A101H"],"award-info":[{"award-number":["NNX15A101H"]}],"id":[{"id":"10.13039\/100000104","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005768","name":"NASA West Virginia Space Grant Consortium","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005768","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"publisher","award":["1R15HL147135"],"award-info":[{"award-number":["1R15HL147135"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.mcp.2024.101956","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T03:46:00Z","timestamp":1710819960000},"page":"101956","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria"],"prefix":"10.1016","volume":"74","author":[{"given":"Kristen","family":"Haggerty","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stuart","family":"Cantlay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariah K.","family":"Cashbaugh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elio F.","family":"Delatore III","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rori","family":"Schreiber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayden","family":"Hess","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel R.","family":"Komlosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Butler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalton","family":"Bolon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa","family":"Evangelista","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takoda","family":"Hager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jada","family":"Voellinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.Q.","family":"Shanks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0867-5099","authenticated-orcid":false,"given":"Joseph","family":"Horzempa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2024.101956_bib1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1126\/science.1124618","article-title":"The fluorescent toolbox for assessing protein location and function","volume":"312","author":"Giepmans","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.mcp.2024.101956_bib2","doi-asserted-by":"crossref","DOI":"10.1038\/nmeth819","article-title":"A guide to choosing fluorescent proteins","author":"Shaner","year":"2005","journal-title":"Nat. Methods"},{"key":"10.1016\/j.mcp.2024.101956_bib3","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1038\/nbt1037","article-title":"Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein","volume":"22","author":"Shaner","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mcp.2024.101956_bib4","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1126\/science.8303295","article-title":"Green fluorescent protein as a marker for gene expression","volume":"263","author":"Chalfie","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.mcp.2024.101956_bib5","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0378-1119(95)00706-7","article-title":"Applications for green fluorescent protein (GFP) in the study of hostpathogen interactions","volume":"173","author":"Valdivia","year":"1996","journal-title":"Gene"},{"key":"10.1016\/j.mcp.2024.101956_bib6","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01862-10","article-title":"Identification and characterization of novel and potent transcription promoters of Francisella tularensis","author":"Zaide","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mcp.2024.101956_bib7","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nri2746","article-title":"Dynamic imaging of host-pathogen interactions in vivo","volume":"10","author":"Coombes","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.mcp.2024.101956_bib8","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/jbiol225","article-title":"No better time to FRET: shedding light on host pathogen interactions","volume":"9","author":"Hayward","year":"2010","journal-title":"J. Biol."},{"key":"10.1016\/j.mcp.2024.101956_bib9","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2014.00035","article-title":"Comparative review of Francisella tularensis and Francisella novicida","author":"Kingry","year":"2014","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.mcp.2024.101956_bib10","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1080\/00365540701402970","article-title":"Tularaemia in an emergent area in Sweden: an analysis of 234 cases in five years","volume":"39","author":"Eliasson","year":"2007","journal-title":"Scand. J. Infect. Dis."},{"key":"10.1016\/j.mcp.2024.101956_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009952","article-title":"The fischer 344 rat reflects human susceptibility to Francisella pulmonary challenge and provides a new platform for virulence and protection studies","volume":"5","author":"Ray","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.mcp.2024.101956_bib12","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1177\/153567600501000405","article-title":"The infectious dose of Francisella tularensis (tularemia)","volume":"10","author":"Jones","year":"2005","journal-title":"Appl. Biosaf."},{"key":"10.1016\/j.mcp.2024.101956_bib13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1093\/infdis\/jir221","article-title":"Invasion of erythrocytes by Francisella tularensis","volume":"204","author":"Horzempa","year":"2011","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.mcp.2024.101956_bib14","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1128\/AEM.02360-07","article-title":"Characterization and application of a glucose-repressible promoter in Francisella tularensis","volume":"74","author":"Horzempa","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mcp.2024.101956_bib15","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1128\/IAI.00043-08","article-title":"Francisella tularensis invasion of lung epithelial cells","volume":"76","author":"Craven","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mcp.2024.101956_bib16","doi-asserted-by":"crossref","first-page":"5843","DOI":"10.1128\/IAI.01176-08","article-title":"Infected-host-cell repertoire and cellular response in the lung following inhalation of Francisella tularensis Schu S4, LVS, or U112","volume":"76","author":"Hall","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mcp.2024.101956_bib17","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2180-6-69","article-title":"Identification of transposon insertion mutants of Francisella tularensis tularensis strain Schu S4 deficient in intracellular replication in the hepatic cell line HepG2","volume":"6","author":"Qin","year":"2006","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.mcp.2024.101956_bib18","doi-asserted-by":"crossref","first-page":"379","DOI":"10.3109\/10520290903504753","article-title":"TdTomato and EGFP identification in histological sections: insight and alternatives","volume":"85","author":"Morris","year":"2010","journal-title":"Biotech. Histochem. Off. Publ. Biol. Stain Comm."},{"key":"10.1016\/j.mcp.2024.101956_bib19","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jbiotec.2023.08.008","article-title":"Functional small peptides for enhanced protein delivery, solubility, and secretion in microbial biotechnology","volume":"375","author":"Lee","year":"2023","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.mcp.2024.101956_bib20","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1007\/s00253-020-10402-8","article-title":"Fusion tags to enhance heterologous protein expression","volume":"104","author":"Ki","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.mcp.2024.101956_bib21","doi-asserted-by":"crossref","first-page":"4614","DOI":"10.1038\/s41467-022-32423-9","article-title":"A 33-residue peptide tag increases solubility and stability of Escherichia coli produced single-chain antibody fragments","volume":"13","author":"Wang","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcp.2024.101956_bib22","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1186\/s12934-020-01371-8","article-title":"The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host's fluorescent intensity","volume":"19","author":"Gao","year":"2020","journal-title":"Microb. Cell Factories"},{"key":"10.1016\/j.mcp.2024.101956_bib23","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.jbiosc.2016.12.004","article-title":"N-terminal SKIK peptide tag markedly improves expression of difficult-to-express proteins in Escherichia coli and Saccharomyces cerevisiae","volume":"123","author":"Ojima-Kato","year":"2017","journal-title":"J. Biosci. Bioeng."},{"key":"10.1016\/j.mcp.2024.101956_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.104676","article-title":"Nascent MSKIK peptide cancels ribosomal stalling by arrest peptides in Escherichia coli","volume":"299","author":"Ojima-Kato","year":"2023","journal-title":"J. Biol. Chem."},{"year":"2018","author":"Balleza","series-title":"Systematic Characterization of Maturation Time of Fluorescent Proteins in Living Cells","key":"10.1016\/j.mcp.2024.101956_bib25"},{"key":"10.1016\/j.mcp.2024.101956_bib26","doi-asserted-by":"crossref","DOI":"10.1128\/JB.00048-20","article-title":"OpiA, a type six secretion system substrate, localizes to the cell Pole and plays a role in bacterial growth and viability in Francisella tularensis LVS","volume":"202","author":"Cantlay","year":"2020","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mcp.2024.101956_bib27","doi-asserted-by":"crossref","first-page":"226","DOI":"10.3389\/fmicb.2012.00226","article-title":"Resistance of Francisella novicida to fosmidomycin associated with mutations in the glycerol-3-phosphate transporter","volume":"3","author":"Mackie","year":"2012","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mcp.2024.101956_bib28","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-47931-w","article-title":"Inhibition of Francisella tularensis phagocytosis using a novel anti-LPS scFv antibody fragment","volume":"9","author":"Mechaly","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcp.2024.101956_bib29","doi-asserted-by":"crossref","DOI":"10.1080\/21655979.2015.1011033","article-title":"Genetic engineering of Francisella tularensis LVS for use as a novel live vaccine platform against Pseudomonas aeruginosa infections","volume":"6","author":"Robinson","year":"2015","journal-title":"Bioengineered"},{"key":"10.1016\/j.mcp.2024.101956_bib30","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1080\/21655979.2015.1011033","article-title":"Genetic engineering of Francisella tularensis LVS for use as a novel live vaccine platform against Pseudomonas aeruginosa infections","volume":"6","author":"Robinson","year":"2015","journal-title":"Bioengineered"},{"key":"10.1016\/j.mcp.2024.101956_bib31","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.1128\/JB.00273-06","article-title":"Pseudomonas aeruginosa 1244 pilin glycosylation: glycan substrate recognition","volume":"188","author":"Horzempa","year":"2006","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mcp.2024.101956_bib32","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1128\/IAI.70.6.2837-2845.2002","article-title":"Identification of the Pseudomonas aeruginosa 1244 pilin glycosylation site","volume":"70","author":"Comer","year":"2002","journal-title":"Infect. Immun."},{"key":"10.1016\/j.mcp.2024.101956_bib33","doi-asserted-by":"crossref","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.mcp.2024.101956_bib34","doi-asserted-by":"crossref","DOI":"10.1128\/jb.00268-22","article-title":"A ribosomal protein homolog governs gene expression and virulence in a bacterial pathogen","volume":"204","author":"Trautmann","year":"2022","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mcp.2024.101956_bib35","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.2186","article-title":"Chase assay of protein stability in haloferax volcanii","volume":"7","author":"Fu","year":"2017","journal-title":"Bio-Protocol."},{"key":"10.1016\/j.mcp.2024.101956_bib36","doi-asserted-by":"crossref","first-page":"W70","DOI":"10.1093\/nar\/gkn188","article-title":"The Vienna RNA websuite","volume":"36","author":"Gruber","year":"2008","journal-title":"Nucleic Acids Res."},{"unstructured":"K. Haggerty, The Development of Molecular Tools and Optimization of in Vitro Methods to Investigate Erythrocyte Invasion by Francisella Tularensis, MS Thesis, West Liberty University, 2020.","key":"10.1016\/j.mcp.2024.101956_bib37"},{"key":"10.1016\/j.mcp.2024.101956_bib38","doi-asserted-by":"crossref","first-page":"D439","DOI":"10.1093\/nar\/gkab1061","article-title":"AlphaFold Protein Structure Database: massively expanding the structural coverage of protein-sequence space with high-accuracy models","volume":"50","author":"Varadi","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcp.2024.101956_bib39","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/nar\/gkv199","article-title":"Trade-offs between tRNA abundance and mRNA secondary structure support smoothing of translation elongation rate","volume":"43","author":"Gorochowski","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcp.2024.101956_bib40","doi-asserted-by":"crossref","DOI":"10.3390\/ijms22157845","article-title":"The role of RNA secondary structure in regulation of gene expression in bacteria","volume":"22","author":"Che\u0142kowska-Pauszek","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcp.2024.101956_bib41","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1128\/JB.186.2.278-286.2004","article-title":"The trp RNA-binding attenuation protein of Bacillus subtilis regulates translation of the tryptophan transport gene trpP (yhaG) by blocking ribosome binding","volume":"186","author":"Yakhnin","year":"2004","journal-title":"J. Bacteriol."},{"volume":"vol. 33","year":"2022","author":"Hector","key":"10.1016\/j.mcp.2024.101956_bib42"},{"key":"10.1016\/j.mcp.2024.101956_bib43","doi-asserted-by":"crossref","DOI":"10.1002\/eji.201040923","article-title":"Persistence of cell-mediated immunity three decades after vaccination with the live vaccine strain of Francisella tularensis","author":"Enesl\u00e4tt","year":"2011","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.mcp.2024.101956_bib44","doi-asserted-by":"crossref","first-page":"7009","DOI":"10.1038\/s41598-018-24581-y","article-title":"Single vector platform vaccine protects against lethal respiratory challenge with Tier 1 select agents of anthrax, plague, and tularemia","volume":"8","author":"Jia","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mcp.2024.101956_bib45","doi-asserted-by":"crossref","first-page":"4969","DOI":"10.1016\/j.vaccine.2016.08.041","article-title":"Francisella tularensis Live Vaccine Strain deficient in capB and overexpressing the fusion protein of IglA, IglB, and IglC from the bfr promoter induces improved protection against F. tularensis respiratory challenge","volume":"34","author":"Jia","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.mcp.2024.101956_bib46","doi-asserted-by":"crossref","first-page":"154","DOI":"10.3389\/fcimb.2018.00154","article-title":"Live attenuated tularemia vaccines for protection against respiratory challenge with virulent F. Tularensis subsp. tularensis","volume":"8","author":"Jia","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850824000082?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850824000082?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T20:35:31Z","timestamp":1717274131000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850824000082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":46,"alternative-id":["S0890850824000082"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2024.101956","relation":{},"ISSN":["0890-8508"],"issn-type":[{"type":"print","value":"0890-8508"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification of an N-terminal tag (580N) that improves the biosynthesis of fluorescent proteins in Francisella tularensis and other Gram-negative bacteria","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2024.101956","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"101956"}}