{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:15:56Z","timestamp":1788264956094,"version":"build-2803163510"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular and Cellular Probes"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.mcp.2026.102085","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:08:36Z","timestamp":1787008116000},"page":"102085","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Integration of PNA-mediated PCR and CRISPR\/Cas13a for highly sensitive detection of EGFR T790M mutation in circulating tumor DNA"],"prefix":"10.1016","volume":"90","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcp.2026.102085_bib1","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-024-55251-5","article-title":"Characterizing mutation-treatment effects using clinico-genomics data of 78,287 patients with 20 types of cancers","volume":"15","author":"Liu","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcp.2026.102085_bib2","doi-asserted-by":"crossref","first-page":"18","DOI":"10.21037\/atm-25-34","article-title":"Advances in personalized medicine: translating genomic insights into targeted therapies for cancer treatment","volume":"13","author":"Jamalinia","year":"2025","journal-title":"Ann. Transl. Med."},{"key":"10.1016\/j.mcp.2026.102085_bib3","first-page":"387","article-title":"Personalized care for patients with EGFR-mutant nonsmall cell lung cancer: navigating early to advanced disease management","volume":"75","author":"Borgeaud","year":"2025","journal-title":"CA Cancer J. Clin."},{"key":"10.1016\/j.mcp.2026.102085_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.drup.2025.101266","article-title":"Molecular mechanisms of acquired resistance to EGFR tyrosine kinase inhibitors in non-small cell lung cancer","volume":"82","author":"Du","year":"2025","journal-title":"Drug Resist. Updates"},{"key":"10.1016\/j.mcp.2026.102085_bib5","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1158\/1078-0432.CCR-12-2246","article-title":"Analysis of tumor specimens at the time of acquired resistance to EGFR-TKI therapy in 155 patients with EGFR-mutant lung cancers","volume":"19","author":"Yu","year":"2025","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.mcp.2026.102085_bib6","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1038\/s41591-024-03216-y","article-title":"Ultrasensitive ctDNA detection for preoperative disease stratification in early-stage lung adenocarcinoma","volume":"31","author":"Black","year":"2025","journal-title":"Nat. Med."},{"key":"10.1016\/j.mcp.2026.102085_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.ctrv.2024.102791","article-title":"Circulating tumor DNA to guide diagnosis and treatment of localized and locally advanced non-small cell lung cancer","volume":"129","author":"Marinello","year":"2024","journal-title":"Cancer Treat Rev."},{"key":"10.1016\/j.mcp.2026.102085_bib8","article-title":"Circulating cell-free DNA for cancer early detection","volume":"3","author":"Gao","year":"2022","journal-title":"Innovation (Camb)"},{"issue":"5","key":"10.1016\/j.mcp.2026.102085_bib9","doi-asserted-by":"crossref","first-page":"986","DOI":"10.21037\/tlcr-23-848","article-title":"Comprehensive analysis of next generation sequencing and ARMS-PCR for detecting EGFR exon 20 insertion (ex20ins) mutations in Chinese non-small cell lung cancer patients","volume":"13","author":"Xu","year":"2024","journal-title":"Transl. Lung Cancer Res."},{"key":"10.1016\/j.mcp.2026.102085_bib10","doi-asserted-by":"crossref","DOI":"10.1172\/JCI154941","article-title":"Circulating tumor DNA: current challenges for clinical utility","volume":"132","author":"Dang","year":"2022","journal-title":"J. Clin. Investig."},{"key":"10.1016\/j.mcp.2026.102085_bib11","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1126\/science.aas8836","article-title":"Field-deployable viral diagnostics using CRISPR-Cas13","volume":"360","author":"Myhrvold","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.mcp.2026.102085_bib12","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1126\/science.aaq0179","article-title":"Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6","volume":"360","author":"Gootenberg","year":"2018","journal-title":"Science"},{"key":"10.1016\/j.mcp.2026.102085_bib13","doi-asserted-by":"crossref","first-page":"2228","DOI":"10.1021\/acssynbio.9b00209","article-title":"HOLMESv2: a CRISPR-Cas12b-Assisted platform for nucleic acid detection and DNA methylation quantitation","volume":"8","author":"Li","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"10.1016\/j.mcp.2026.102085_bib14","article-title":"SATCAS: a CRISPR\/Cas13a-based simultaneous amplification and testing platform for one-pot RNA detection and SNPs distinguish in clinical diagnosis","volume":"263","author":"Wang","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.mcp.2026.102085_bib15","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1126\/science.aam9321","article-title":"Nucleic acid detection with CRISPR-Cas13a\/C2c2","volume":"356","author":"Gootenberg","year":"2017","journal-title":"Science"},{"issue":"6387","key":"10.1016\/j.mcp.2026.102085_bib16","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1126\/science.aat4982","article-title":"Next-generation diagnostics with CRISPR","volume":"360","author":"Chertow","year":"2018","journal-title":"Science"},{"issue":"12","key":"10.1016\/j.mcp.2026.102085_bib17","article-title":"Synthetic mismatches enable specific CRISPR-Cas12a-based detection of genome-wide SNVs tracked by ARTEMIS","volume":"4","author":"Kohabir","year":"2024","journal-title":"Cell Rep. Methods"},{"key":"10.1016\/j.mcp.2026.102085_bib18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s12943-021-01456-x","article-title":"EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations","volume":"20","author":"Liu","year":"2021","journal-title":"Mol. Cancer"},{"key":"10.1016\/j.mcp.2026.102085_bib19","article-title":"A novel platform for mutation detection in colorectal cancer using a PNA-LNA molecular switch","volume":"267","author":"Islam","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"10.1016\/j.mcp.2026.102085_bib20","doi-asserted-by":"crossref","first-page":"14209","DOI":"10.1021\/acs.analchem.3c01809","article-title":"PNA-Pdx: versatile peptide nucleic acid-based detection of nucleic acids and SNPs","volume":"95","author":"Jiang","year":"2023","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mcp.2026.102085_bib21","doi-asserted-by":"crossref","first-page":"786","DOI":"10.3390\/molecules25040786","article-title":"PNA clamping in nucleic acid amplification protocols to detect single nucleotide mutations related to cancer","volume":"25","author":"Fouz","year":"2020","journal-title":"Molecules"},{"key":"10.1016\/j.mcp.2026.102085_bib22","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/s42003-024-06368-2","article-title":"CRISPR\/Cas13a-based supersensitive circulating tumor DNA assay for detecting EGFR mutations in plasma","volume":"7","author":"Wang","year":"2024","journal-title":"Commun. Biol."},{"key":"10.1016\/j.mcp.2026.102085_bib23","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/10408363.2023.2275150","article-title":"Circulating tumor DNA (ctDNA): can it be used as a pan-cancer early detection test?","volume":"61","author":"Duffy","year":"2024","journal-title":"Crit. Rev. Clin. Lab Sci."},{"key":"10.1016\/j.mcp.2026.102085_bib24","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s43018-020-0043-5","article-title":"Circulating tumor DNA and liquid biopsy in oncology","volume":"1","author":"Cescon","year":"2020","journal-title":"Nat. Cancer"},{"key":"10.1016\/j.mcp.2026.102085_bib25","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1021\/acssensors.7b00953","article-title":"Extending circulating tumor DNA analysis to ultralow abundance mutations: techniques and challenges","volume":"3","author":"Rodda","year":"2018","journal-title":"ACS Sens."},{"key":"10.1016\/j.mcp.2026.102085_bib26","doi-asserted-by":"crossref","first-page":"5597","DOI":"10.1038\/s41467-025-60917-9","article-title":"Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection","volume":"16","author":"Lin","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcp.2026.102085_bib27","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1186\/s12985-025-02938-w","article-title":"Development of a single-tube, dual-target CRISPR Cas12a\/Cas13a system for rapid screening of coinfection with respiratory syncytial virus and rhinovirus","volume":"22","author":"Zhao","year":"2025","journal-title":"Virol. J."}],"container-title":["Molecular and Cellular Probes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850826000253?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0890850826000253?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:35:11Z","timestamp":1788262511000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0890850826000253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":27,"alternative-id":["S0890850826000253"],"URL":"https:\/\/doi.org\/10.1016\/j.mcp.2026.102085","relation":{},"ISSN":["0890-8508"],"issn-type":[{"value":"0890-8508","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integration of PNA-mediated PCR and CRISPR\/Cas13a for highly sensitive detection of EGFR T790M mutation in circulating tumor DNA","name":"articletitle","label":"Article Title"},{"value":"Molecular and Cellular Probes","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcp.2026.102085","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"102085"}}