{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:18:35Z","timestamp":1792034315565,"version":"4.4.0"},"reference-count":32,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFA1309201"],"award-info":[{"award-number":["2025YFA1309201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular & Cellular Proteomics"],"published-print":{"date-parts":[[2026,7]]},"DOI":"10.1016\/j.mcpro.2026.101595","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T16:20:35Z","timestamp":1779812435000},"page":"101595","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated IP-MS and Optoproteomics Reveal Dynamic Remodeling of Protein Interaction Networks During NLRP3 Inflammasome Activation"],"prefix":"10.1016","volume":"25","author":[{"given":"Yuehui","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kairong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaozhi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanzhi","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8116-7691","authenticated-orcid":false,"given":"Jian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcpro.2026.101595_bib1","doi-asserted-by":"crossref","DOI":"10.1111\/imr.13406","article-title":"Molecular mechanisms of emerging inflammasome complexes and their activation and signaling in inflammation and pyroptosis","volume":"329","author":"Pandey","year":"2025","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.mcpro.2026.101595_bib2","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cell.2016.03.046","article-title":"Inflammasome complexes: emerging mechanisms and effector functions","volume":"165","author":"Rathinam","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.mcpro.2026.101595_bib3","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.tibs.2022.10.002","article-title":"The NLRP3 inflammasome: activation and regulation","volume":"48","author":"Xu","year":"2023","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.mcpro.2026.101595_bib4","doi-asserted-by":"crossref","first-page":"787","DOI":"10.4049\/jimmunol.0901363","article-title":"Cutting edge: NF-kappaB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression","volume":"183","author":"Bauernfeind","year":"2009","journal-title":"J. Immunol."},{"key":"10.1016\/j.mcpro.2026.101595_bib5","doi-asserted-by":"crossref","first-page":"3328","DOI":"10.3390\/ijms20133328","article-title":"The NLRP3 inflammasome: an overview of mechanisms of activation and regulation","volume":"20","author":"Kelley","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.mcpro.2026.101595_bib6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.smim.2018.09.001","article-title":"NLRP3 inflammasome activation in inflammaging","volume":"40","author":"Latz","year":"2018","journal-title":"Semin. Immunol."},{"key":"10.1016\/j.mcpro.2026.101595_bib7","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1038\/cr.2015.139","article-title":"Gasdermin D is an executor of pyroptosis and required for interleukin-1beta secretion","volume":"25","author":"He","year":"2015","journal-title":"Cell Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib8","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkaf554","article-title":"The inflammasome sensor NLRP3 interacts with REV7 to maintain genome integrity through homologous recombination","volume":"53","author":"Burlet","year":"2025","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108405","article-title":"RACK1 mediates NLRP3 inflammasome activation by promoting NLRP3 active conformation and inflammasome assembly","volume":"33","author":"Duan","year":"2020","journal-title":"Cell Rep."},{"key":"10.1016\/j.mcpro.2026.101595_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.xpro.2024.103417","article-title":"Protocol to profile spatially resolved NLRP3 inflammasome complexes using APEX2-based proximity labeling","volume":"5","author":"Liang","year":"2024","journal-title":"STAR Protoc."},{"key":"10.1016\/j.mcpro.2026.101595_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2024.114152","article-title":"Proximity proteomics reveals UCH-L1 as an essential regulator of NLRP3-mediated IL-1beta production in human macrophages and microglia","volume":"43","author":"Liang","year":"2024","journal-title":"Cell Rep."},{"key":"10.1016\/j.mcpro.2026.101595_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcpro.2025.100972","article-title":"Thermal proteome profiling reveals meltome upon NLRP3 inflammasome activation","volume":"24","author":"Yang","year":"2025","journal-title":"Mol. Cell. Proteomics : MCP"},{"key":"10.1016\/j.mcpro.2026.101595_bib13","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1002\/pmic.201100167","article-title":"PepDistiller: a quality control tool to improve the sensitivity and accuracy of peptide identifications in shotgun proteomics","volume":"12","author":"Li","year":"2012","journal-title":"Proteomics"},{"key":"10.1016\/j.mcpro.2026.101595_bib14","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1093\/bioinformatics\/bty727","article-title":"PANDA: a comprehensive and flexible tool for quantitative proteomics data analysis","volume":"35","author":"Chang","year":"2019","journal-title":"Bioinformatics"},{"key":"10.1016\/j.mcpro.2026.101595_bib15","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jprot.2013.10.023","article-title":"SAINTexpress: improvements and additional features in Significance Analysis of INTeractome software","volume":"100","author":"Teo","year":"2014","journal-title":"J. Proteomics"},{"key":"10.1016\/j.mcpro.2026.101595_bib16","doi-asserted-by":"crossref","DOI":"10.1126\/science.abf2911","article-title":"A protein network map of head and neck cancer reveals PIK3CA mutant drug sensitivity","volume":"374","author":"Swaney","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.mcpro.2026.101595_bib17","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib18","article-title":"clusterProfiler 4.0: a universal enrichment tool for interpreting omics data","volume":"2","author":"Wu","year":"2021","journal-title":"Innovation"},{"key":"10.1016\/j.mcpro.2026.101595_bib19","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/pro.3978","article-title":"The BioGRID database: a comprehensive biomedical resource of curated protein, genetic, and chemical interactions","volume":"30","author":"Oughtred","year":"2020","journal-title":"Protein Sci."},{"key":"10.1016\/j.mcpro.2026.101595_bib20","doi-asserted-by":"crossref","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING database in 2023: protein\u2013protein association networks and functional enrichment analyses for any sequenced genome of interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib21","doi-asserted-by":"crossref","first-page":"D648","DOI":"10.1093\/nar\/gkab1006","article-title":"The IntAct database: efficient access to fine-grained molecular interaction data","volume":"50","author":"Del Toro","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib22","doi-asserted-by":"crossref","DOI":"10.1172\/JCI161935","article-title":"Posttranslational ISGylation of NLRP3 by HERC enzymes facilitates inflammasome activation in models of inflammation","volume":"133","author":"Qin","year":"2023","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.mcpro.2026.101595_bib23","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1038\/s41467-021-25033-4","article-title":"TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation","volume":"12","author":"Qin","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101595_bib24","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1189\/jlb.3HI0814-371RR","article-title":"Syk is involved in NLRP3 inflammasome-mediated caspase-1 activation through adaptor ASC phosphorylation and enhanced oligomerization","volume":"97","author":"Lin","year":"2015","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.mcpro.2026.101595_bib25","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/icb.2011.72","article-title":"Mycobacterium abscessus activates the NLRP3 inflammasome via Dectin-1-Syk and p62\/SQSTM1","volume":"90","author":"Lee","year":"2012","journal-title":"Immunol. Cell Biol."},{"key":"10.1016\/j.mcpro.2026.101595_bib26","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2123247119","article-title":"Mitochondrial calcium uniporter promotes phagocytosis-dependent activation of the NLRP3 inflammasome","volume":"119","author":"Dong","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mcpro.2026.101595_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2022.102265","article-title":"Genetic BACH1 deficiency alters mitochondrial function and increases NLRP3 inflammasome activation in mouse macrophages","volume":"51","author":"Pradhan","year":"2022","journal-title":"Redox Biol."},{"key":"10.1016\/j.mcpro.2026.101595_bib28","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1084\/jem.20141461","article-title":"Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses","volume":"214","author":"Westphal","year":"2017","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.mcpro.2026.101595_bib29","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1016\/j.immuni.2013.05.016","article-title":"K(+) efflux is the common trigger of NLRP3 inflammasome activation by bacterial toxins and particulate matter","volume":"38","author":"Munoz-Planillo","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.mcpro.2026.101595_bib30","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1016\/j.cell.2014.02.008","article-title":"Unified polymerization mechanism for the assembly of ASC-dependent inflammasomes","volume":"156","author":"Lu","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.mcpro.2026.101595_bib31","doi-asserted-by":"crossref","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","article-title":"iProX in 2021: connecting proteomics data sharing with big data","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.mcpro.2026.101595_bib32","doi-asserted-by":"crossref","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: an integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["Molecular & Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947626000915?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947626000915?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T12:06:39Z","timestamp":1785326799000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947626000915"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["S1535947626000915"],"URL":"https:\/\/doi.org\/10.1016\/j.mcpro.2026.101595","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated IP-MS and Optoproteomics Reveal Dynamic Remodeling of Protein Interaction Networks During NLRP3 Inflammasome Activation","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcpro.2026.101595","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101595"}}