{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:58:22Z","timestamp":1790416702954,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Beijing Natural Science Foundation","doi-asserted-by":"publisher","award":["7252135"],"award-info":[{"award-number":["7252135"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170724"],"award-info":[{"award-number":["82170724"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82570843"],"award-info":[{"award-number":["82570843"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["mcponline.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Molecular & Cellular Proteomics"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.mcpro.2026.101652","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:03:35Z","timestamp":1788307415000},"page":"101652","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mass Spectrometry-Based De Novo Sequencing of N-Glycosylated Light Chains: Linking Urinary Proteoforms to In Situ Renal Amyloid Deposits"],"prefix":"10.1016","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1498-823X","authenticated-orcid":false,"given":"Yueyue","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7768-0632","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-7416","authenticated-orcid":false,"given":"Peifeng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zidi","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7631-6464","authenticated-orcid":false,"given":"Suxia","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mcpro.2026.101652_bib1","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1056\/NEJMra2304088","article-title":"Systemic light chain amyloidosis","volume":"390","author":"Sanchorawala","year":"2024","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.mcpro.2026.101652_bib2","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1146\/annurev-cellbio-112122-030835","article-title":"Diversification of antibodies: from V(D)J recombination to somatic exon shuffling","volume":"40","author":"Lebedin","year":"2024","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.mcpro.2026.101652_bib3","series-title":"Janeway's Immunobiology","author":"Murphy","year":"2017"},{"key":"10.1016\/j.mcpro.2026.101652_bib4","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1038\/s41467-021-23880-9","article-title":"Machine learning analyses of antibody somatic mutations predict immunoglobulin light chain toxicity","volume":"12","author":"Garofalo","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101652_bib5","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1080\/13506129.2023.2212397","article-title":"Role of complementarity-determining regions 1 and 3 in pathologic amyloid formation by human immunoglobulin \u03ba1 light chains","volume":"30","author":"Klimtchuk","year":"2023","journal-title":"Amyloid"},{"key":"10.1016\/j.mcpro.2026.101652_bib6","doi-asserted-by":"crossref","first-page":"5121","DOI":"10.1038\/s41467-024-49520-6","article-title":"Light chain mutations contribute to defining the fibril morphology in systemic AL amyloidosis","volume":"15","author":"Karimi-Farsijani","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101652_bib7","doi-asserted-by":"crossref","first-page":"6359","DOI":"10.1038\/s41467-024-50686-2","article-title":"Helical superstructures between amyloid and collagen in cardiac fibrils from a patient with AL amyloidosis","volume":"15","author":"Schulte","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101652_bib8","doi-asserted-by":"crossref","first-page":"6434","DOI":"10.1038\/s41467-021-26553-9","article-title":"Role of mutations and post-translational modifications in systemic AL amyloidosis studied by cryo-EM","volume":"12","author":"Radamaker","year":"2021","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101652_bib9","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/s41375-018-0194-x","article-title":"Assay to rapidly screen for immunoglobulin light chain glycosylation: a potential path to earlier AL diagnosis for a subset of patients","volume":"33","author":"Kumar","year":"2019","journal-title":"Leukemia"},{"key":"10.1016\/j.mcpro.2026.101652_bib10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/s41408-021-00444-0","article-title":"MASS-FIX for the detection of monoclonal proteins and light chain N-glycosylation in routine clinical practice: a cross-sectional study of 6315 patients","volume":"11","author":"Mellors","year":"2021","journal-title":"Blood Cancer J."},{"key":"10.1016\/j.mcpro.2026.101652_bib11","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.1038\/s41375-022-01599-w","article-title":"An N-glycosylation hotspot in immunoglobulin \u03ba light chains is associated with AL amyloidosis","volume":"36","author":"Nevone","year":"2022","journal-title":"Leukemia"},{"key":"10.1016\/j.mcpro.2026.101652_bib12","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1038\/s41375-020-0940-8","article-title":"N-glycosylation of monoclonal light chains on routine MASS-FIX testing is a risk factor for MGUS progression","volume":"34","author":"Dispenzieri","year":"2020","journal-title":"Leukemia"},{"key":"10.1016\/j.mcpro.2026.101652_bib13","doi-asserted-by":"crossref","first-page":"E368","DOI":"10.1002\/ajh.25244","article-title":"MASS-FIX may allow identification of patients at risk for light chain amyloidosis before the onset of symptoms","volume":"93","author":"Kourelis","year":"2018","journal-title":"Am. J. Hematol."},{"key":"10.1016\/j.mcpro.2026.101652_bib14","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.kint.2021.10.017","article-title":"RNA-based immunoglobulin repertoire sequencing is a new tool for the management of monoclonal gammopathy of renal (kidney) significance","volume":"101","author":"Javaugue","year":"2022","journal-title":"Kidney Int."},{"key":"10.1016\/j.mcpro.2026.101652_bib15","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1080\/13506129.2022.2095618","article-title":"Identification of AL proteins from 10 \u03bb-AL amyloidosis patients by mass spectrometry extracted from abdominal fat and heart tissue","volume":"30","author":"Baur","year":"2023","journal-title":"Amyloid"},{"key":"10.1016\/j.mcpro.2026.101652_bib16","doi-asserted-by":"crossref","first-page":"10627","DOI":"10.1021\/acs.analchem.1c01955","article-title":"De Novo sequencing of antibody light chain proteoforms from patients with multiple myeloma","volume":"93","author":"Dupr\u00e9","year":"2021","journal-title":"Anal. Chem."},{"key":"10.1016\/j.mcpro.2026.101652_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.intimp.2024.112302","article-title":"Immunoglobulin repertoire sequencing and de novo sequencing \u2013 powerful tools for identifying free light chains from patients with light chain cast nephropathy","volume":"135","author":"Wang","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"10.1016\/j.mcpro.2026.101652_bib18","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1021\/jacsau.4c00960","article-title":"Decoding protein glycosylation by an integrative mass spectrometry-based de novo sequencing strategy","volume":"5","author":"Gao","year":"2025","journal-title":"JACS Au"},{"key":"10.1016\/j.mcpro.2026.101652_bib19","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1182\/blood-2014-04-570010","article-title":"A staging system for renal outcome and early markers of renal response to chemotherapy in AL amyloidosis","volume":"124","author":"Palladini","year":"2014","journal-title":"Blood"},{"key":"10.1016\/j.mcpro.2026.101652_bib20","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1074\/mcp.M112.025221","article-title":"Nepenthesin from monkey cups for hydrogen\/deuterium exchange mass spectrometry","volume":"12","author":"Rey","year":"2013","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.mcpro.2026.101652_bib21","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1006\/abio.2001.5199","article-title":"Extension of the in-gel release method for structural analysis of neutral and sialylated N-linked glycans to the analysis of sulfated glycans: application to the glycans from bovine thyroid-stimulating hormone","volume":"296","author":"Wheeler","year":"2001","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mcpro.2026.101652_bib22","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1006\/abio.1997.2199","article-title":"Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption\/ionization mass spectrometry and normal-phase high-performance liquid chromatography","volume":"250","author":"K\u00fcster","year":"1997","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mcpro.2026.101652_bib23","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1007\/s13361-014-0892-1","article-title":"A quantitative tool to distinguish isobaric leucine and isoleucine residues for mass spectrometry-based de novo monoclonal antibody sequencing","volume":"25","author":"Poston","year":"2014","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.mcpro.2026.101652_bib24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1080\/13506129.2024.2434899","article-title":"An updated AL-base reveals ranked enrichment of immunoglobulin light chain variable genes in AL amyloidosis","volume":"32","author":"Morgan","year":"2025","journal-title":"Amyloid"},{"key":"10.1016\/j.mcpro.2026.101652_bib25","doi-asserted-by":"crossref","first-page":"1885","DOI":"10.1007\/s13361-015-1204-0","article-title":"Novor: real-time peptide de novo sequencing software","volume":"26","author":"Ma","year":"2015","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"10.1016\/j.mcpro.2026.101652_bib26","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1080\/13506129.2026.2619419","article-title":"Clarifying the immunoglobulin light chain variable gene usage in Chinese patients with renal AL amyloidosis","volume":"33","author":"Wang","year":"2026","journal-title":"Amyloid"},{"key":"10.1016\/j.mcpro.2026.101652_bib27","doi-asserted-by":"crossref","first-page":"S193","DOI":"10.1074\/mcp.TIR118.001209","article-title":"Interactive peptide spectral annotator: a versatile web-based tool for proteomic applications","volume":"18","author":"Brademan","year":"2019","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.mcpro.2026.101652_bib28","doi-asserted-by":"crossref","DOI":"10.1038\/s42003-026-10589-y","article-title":"Cathepsin resistance mediated by key amino acid substitutions drives intracellular crystallization in light chain proximal tubulopathy","author":"Wang","year":"2026","journal-title":"Commun. Biol."},{"key":"10.1016\/j.mcpro.2026.101652_bib29","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1373\/clinchem.2015.253781","article-title":"Screening method for M-Proteins in serum using nanobody enrichment coupled to MALDI-TOF mass spectrometry","volume":"62","author":"Kohlhagen","year":"2016","journal-title":"Clin. Chem."},{"key":"10.1016\/j.mcpro.2026.101652_bib30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.clinbiochem.2022.09.004","article-title":"Characterizing M-protein light chain glycosylation via mass spectrometry","volume":"109\u2013110","author":"Miller","year":"2022","journal-title":"Clin. Biochem."},{"key":"10.1016\/j.mcpro.2026.101652_bib31","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/978-1-0716-1685-7_2","article-title":"GlycoStore: a platform for H\/UPLC and capillary electrophoresis Glycan data","volume":"2370","author":"Campbell","year":"2022","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.mcpro.2026.101652_bib32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1021\/acs.jproteome.6b00608","article-title":"De Novo MS\/MS Sequencing of Native Human Antibodies","volume":"16","author":"Guthals","year":"2017","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.mcpro.2026.101652_bib33","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.3390\/ph17111542","article-title":"Predicting structural consequences of antibody light chain N-Glycosylation in AL amyloidosis","volume":"17","author":"Morgan","year":"2024","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.mcpro.2026.101652_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.str.2026.05.011","article-title":"Multifaceted effects of N-glycosylation on amyloidogenic \u03ba light chains in AL amyloidosis","author":"Puri","year":"2026","journal-title":"Structure"},{"key":"10.1016\/j.mcpro.2026.101652_bib35","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/j.cels.2021.08.008","article-title":"Human plasma IgG1 repertoires are simple, unique, and dynamic","volume":"12","author":"Bondt","year":"2021","journal-title":"Cell Syst"},{"key":"10.1016\/j.mcpro.2026.101652_bib36","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.1021\/acs.jproteome.1c00169","article-title":"Mass spectrometry-based de novo sequencing of monoclonal antibodies using multiple proteases and a dual fragmentation scheme","volume":"20","author":"Peng","year":"2021","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.mcpro.2026.101652_bib37","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1021\/acs.jproteome.3c00330","article-title":"Direct mass spectrometry-based detection and antibody sequencing of monoclonal gammopathy of undetermined significance from patient serum: a case Study","volume":"22","author":"Peng","year":"2023","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.mcpro.2026.101652_bib38","doi-asserted-by":"crossref","first-page":"5028","DOI":"10.1158\/1078-0432.CCR-21-0649","article-title":"A personalized mass spectrometry\u2013based assay to monitor M-protein in patients with multiple myeloma (EasyM)","volume":"27","author":"Liyasova","year":"2021","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.mcpro.2026.101652_bib39","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1158\/1078-0432.CCR-23-2767","article-title":"Monitoring minimal residual disease in patients with multiple myeloma by targeted tracking serum M-Protein using mass spectrometry (EasyM)","volume":"30","author":"Fan","year":"2024","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.mcpro.2026.101652_bib40","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/s41467-025-67556-0","article-title":"Cryo-EM structure of renal AL amyloid fibrils from a patient with \u03bb1 light chain amyloidosis","volume":"17","author":"Yu","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mcpro.2026.101652_bib41","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2515454123","article-title":"Biopsy-resolved cryo-EM structures of amyloid fibrils provide molecular insights into AL amyloidosis","volume":"123","author":"Yao","year":"2026","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mcpro.2026.101652_bib42","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1111\/febs.16182","article-title":"Protease-sensitive regions in amyloid light chains: what a common pattern of fragmentation across organs suggests about aggregation","volume":"289","author":"Mazzini","year":"2022","journal-title":"FEBS J."},{"key":"10.1016\/j.mcpro.2026.101652_bib43","doi-asserted-by":"crossref","first-page":"4957","DOI":"10.1182\/blood-2009-07-230722","article-title":"Classification of amyloidosis by laser microdissection and mass spectrometry\u2013based proteomic analysis in clinical biopsy specimens","volume":"114","author":"Vrana","year":"2009","journal-title":"Blood"},{"key":"10.1016\/j.mcpro.2026.101652_bib44","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1073\/pnas.1711720115","article-title":"Adaptive antibody diversification through N -linked glycosylation of the immunoglobulin variable region","volume":"115","author":"Van De Bovenkamp","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mcpro.2026.101652_bib45","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41408-021-00408-4","article-title":"Mass spectrometry for the evaluation of monoclonal proteins in multiple myeloma and related disorders: an International Myeloma Working Group Mass Spectrometry Committee Report","volume":"11","author":"Murray","year":"2021","journal-title":"Blood Cancer J."}],"container-title":["Molecular & Cellular Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947626001489?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1535947626001489?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:07:50Z","timestamp":1790413670000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1535947626001489"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["S1535947626001489"],"URL":"https:\/\/doi.org\/10.1016\/j.mcpro.2026.101652","relation":{},"ISSN":["1535-9476"],"issn-type":[{"value":"1535-9476","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mass Spectrometry-Based De Novo Sequencing of N-Glycosylated Light Chains: Linking Urinary Proteoforms to In Situ Renal Amyloid Deposits","name":"articletitle","label":"Article Title"},{"value":"Molecular & Cellular Proteomics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mcpro.2026.101652","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}],"article-number":"101652"}}