{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:17:25Z","timestamp":1791875845383,"version":"4.3.4"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"publisher","award":["EXC 2124"],"award-info":[{"award-number":["EXC 2124"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003542","name":"State of Baden-Wurttemberg Ministry for Science Research and Art","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"BMBF Bonn","doi-asserted-by":"publisher","award":["031B0851C"],"award-info":[{"award-number":["031B0851C"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"publisher","award":["NNF21SA0072700"],"award-info":[{"award-number":["NNF21SA0072700"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering Communications"],"published-print":{"date-parts":[[2026,6]]},"DOI":"10.1016\/j.mec.2026.e00275","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:40:35Z","timestamp":1773826835000},"page":"e00275","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Metabolic engineering of Methanothermobacter thermautotrophicus \u0394H for recombinant acetoin production"],"prefix":"10.1016","volume":"22","author":[{"given":"Tina","family":"Baur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilliene T.","family":"Allaart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Zipperle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Contreras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Fink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Largus T.","family":"Angenent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0776-1668","authenticated-orcid":false,"given":"Bastian","family":"Molitor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mec.2026.e00275_bib1","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1007\/s12033-015-9882-1","article-title":"Cloning and overexpression of the als, pflA, and adhB genes in Streptococcus thermophilus and their effects on metabolite formation","volume":"57","author":"Akyol","year":"2015","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib2","doi-asserted-by":"crossref","first-page":"3682","DOI":"10.1039\/D3EE01091K","article-title":"Electrical-energy storage into chemical-energy carriers by combining or integrating electrochemistry and biology","volume":"17","author":"Angenent","year":"2024","journal-title":"Energy Environ. Sci."},{"key":"10.1016\/j.mec.2026.e00275_bib3","series-title":"Resource Recovery from Water: Principles and Application","article-title":"Upgrading anaerobic digestion within the energy economy \u2013 the methane platform","author":"Angenent","year":"2022"},{"key":"10.1016\/j.mec.2026.e00275_bib4","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1038\/nbt1034","article-title":"Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus","volume":"22","author":"Bolotin","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib5","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.108016","article-title":"An integrated systems-biology approach reveals differences in formate metabolism in the genus Methanothermobacter","volume":"26","author":"Casini","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.mec.2026.e00275_bib6","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.crbiot.2022.10.001","article-title":"New perspectives for biotechnological applications of methanogens","volume":"4","author":"Contreras","year":"2022","journal-title":"Curr. Res. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib7","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1080\/07388551.2021.1995319","article-title":"Advances in biological production of acetoin: a comprehensive overview","volume":"42","author":"Cui","year":"2022","journal-title":"Crit. Rev. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib8","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1186\/s12934-024-02492-0","article-title":"An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis","volume":"23","author":"Du","year":"2024","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.mec.2026.e00275_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13568-017-0531-x","article-title":"Methanogens: biochemical background and biotechnological applications","volume":"8","author":"Enzmann","year":"2018","journal-title":"AMB Express"},{"key":"10.1016\/j.mec.2026.e00275_bib10","series-title":"Archaea: Methods and Protocols","first-page":"119","article-title":"An interdomain conjugation protocol for plasmid-DNA transfer into Methanothermobacter thermautotrophicus \u0394H","author":"Fink","year":"2022"},{"key":"10.1016\/j.mec.2026.e00275_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.02766-21","article-title":"A shuttle-vector system allows heterologous gene expression in the thermophilic methanogen Methanothermobacter thermautotrophicus \u0394H","volume":"12","author":"Fink","year":"2021","journal-title":"mBio"},{"key":"10.1016\/j.mec.2026.e00275_bib12","doi-asserted-by":"crossref","DOI":"10.1128\/aem.00575-23","article-title":"The targeted deletion of genes responsible for expression of the Mth60 fimbriae leads to loss of cell-cell connections in Methanothermobacter thermautotrophicus \u0394H","volume":"89","author":"Fink","year":"2023","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.mec.2026.e00275_bib13","series-title":"Molecular Cloning. A Laboratory Manual 4th. 448","author":"Green","year":"2012"},{"key":"10.1016\/j.mec.2026.e00275_bib14","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1155\/2008\/534081","article-title":"New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species","volume":"2","author":"Guss","year":"2008","journal-title":"Archaea"},{"key":"10.1016\/j.mec.2026.e00275_bib15","doi-asserted-by":"crossref","DOI":"10.1093\/femsml\/uqae019","article-title":"Compiling a versatile toolbox for inducible gene expression in Methanosarcina mazei","volume":"5","author":"H\u00fcttermann","year":"2024","journal-title":"microLife"},{"key":"10.1016\/j.mec.2026.e00275_bib16","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/s13568-017-0469-z","article-title":"Application of the thermostable \u03b2-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions","volume":"7","author":"Jensen","year":"2017","journal-title":"AMB Express"},{"key":"10.1016\/j.mec.2026.e00275_bib17","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1038\/s41598-017-04684-8","article-title":"A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate","volume":"7","author":"Jia","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mec.2026.e00275_bib18","doi-asserted-by":"crossref","DOI":"10.1155\/2011\/973848","article-title":"More than 200 genes required for methane formation from H2 and CO2 and energy conservation are present in Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus","volume":"2011","author":"Kaster","year":"2011","journal-title":"Archaea"},{"key":"10.1016\/j.mec.2026.e00275_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2024.1398467","article-title":"Genetic engineering of a thermophilic acetogen, Moorella thermoacetica Y72, to enable acetoin production","volume":"12","author":"Kato","year":"2024","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib20","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03068-24","article-title":"Markerless mutagenesis enables isoleucine biosynthesis solely from threonine in Methanothermobacter marburgensis","volume":"13","author":"Klein","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.mec.2026.e00275_bib21","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.copbio.2019.12.019","article-title":"Harnessing the metabolic potential of Streptococcus thermophilus for new biotechnological applications","volume":"61","author":"Markakiou","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib22","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/157529","article-title":"A single-culture bioprocess of Methanothermobacter thermautotrophicus to upgrade digester biogas by CO2-to-CH4 conversion with H2","volume":"2013","author":"Martin","year":"2013","journal-title":"Archaea"},{"key":"10.1016\/j.mec.2026.e00275_bib23","series-title":"Harnessing the Metabolic Potential of Thermophilic Bacteria: Systems Metabolic Engineering Tools for the Production of Volatile Biochemicals","author":"Mol","year":"2022"},{"key":"10.1016\/j.mec.2026.e00275_bib24","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1046\/j.1472-765X.2003.01326.x","article-title":"Regulation of branched\u2010chain amino acid biosynthesis by \u03b1\u2010acetolactate decarboxylase in Streptococcus thermophilus","volume":"36","author":"Monnet","year":"2003","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.mec.2026.e00275_bib25","series-title":"Methanothermobacter thermautotrophicus and Alternative Methanogens: Archaea-Based Production. Adv. Biochem. Eng. Biotechnol","author":"M\u00fchling","year":"2024"},{"key":"10.1016\/j.mec.2026.e00275_bib26","article-title":"Archaea biotechnology","volume":"47","author":"Pfeifer","year":"2020","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.mec.2026.e00275_bib27","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1111\/1462-2920.12892","article-title":"Distinct mechanisms contribute to immunity in the lantibiotic NAI\u2010107 producer strain M. icrobispora ATCC PTA\u20105024","volume":"18","author":"Pozzi","year":"2016","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.mec.2026.e00275_bib28","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1111\/j.1365-2958.2005.04616.x","article-title":"Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A","volume":"56","author":"Pritchett","year":"2005","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mec.2026.e00275_bib29","doi-asserted-by":"crossref","first-page":"2558","DOI":"10.3168\/jds.S0022-0302(86)80701-9","article-title":"Influence of temperature on associative growth of Streptococcus thermophilus and Lactobacillus bulgaricus","volume":"69","author":"Radke-Mitchell","year":"1986","journal-title":"J. Dairy Sci."},{"key":"10.1016\/j.mec.2026.e00275_bib30","doi-asserted-by":"crossref","first-page":"7834","DOI":"10.1073\/pnas.92.17.7834","article-title":"Multiple Bcl-2 family members demonstrate selective dimerizations with bax","volume":"92","author":"Sedlak","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.mec.2026.e00275_bib31","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2216244120","article-title":"Genome-wide CRISPRi screen identifies enhanced autolithotrophic phenotypes in acetogenic bacterium Eubacterium limosum","volume":"120","author":"Shin","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.mec.2026.e00275_bib32","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1038\/nbt1183-784","article-title":"A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria","volume":"1","author":"Simon","year":"1983","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00275_bib33","doi-asserted-by":"crossref","DOI":"10.1128\/msystems.01159-22","article-title":"Lipidomics and comparative metabolite excretion analysis of methanogenic archaea reveal organism-specific adaptations to varying temperatures and substrate concentrations","volume":"8","author":"Taubner","year":"2023","journal-title":"mSystems"},{"key":"10.1016\/j.mec.2026.e00275_bib34","doi-asserted-by":"crossref","first-page":"15084","DOI":"10.1073\/pnas.1213193109","article-title":"The wolfe cycle comes full circle","volume":"109","author":"Thauer","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.mec.2026.e00275_bib35","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/nrmicro1931","article-title":"Methanogenic archaea: ecologically relevant differences in energy conservation","volume":"6","author":"Thauer","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mec.2026.e00275_bib36","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.rser.2019.06.030","article-title":"Power-to-gas: electrolysis and methanation status review","volume":"112","author":"Thema","year":"2019","journal-title":"Renew. Sustain. Energy Rev."},{"key":"10.1016\/j.mec.2026.e00275_bib37","article-title":"Valine overproduction with metabolically engineered Methanothermobacter marburgensis","author":"Unger","year":"2025","journal-title":"bioRxiv"},{"key":"10.1016\/j.mec.2026.e00275_bib38","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1126\/science.abg5550","article-title":"Three-megadalton complex of methanogenic electron-bifurcating and CO2-fixing enzymes","volume":"373","author":"Watanabe","year":"2021","journal-title":"Science"},{"key":"10.1016\/j.mec.2026.e00275_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13068-019-1512-x","article-title":"Efficient biochemical production of acetoin from carbon dioxide using Cupriavidus necator H16","volume":"12","author":"Windhorst","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"10.1016\/j.mec.2026.e00275_bib40","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3389\/fenrg.2020.00191","article-title":"Review of power-to-x demonstration projects in Europe","volume":"8","author":"Wulf","year":"2020","journal-title":"Front. Energy Res."},{"key":"10.1016\/j.mec.2026.e00275_bib41","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.biotechadv.2014.01.002","article-title":"Strategies for enhancing fermentative production of acetoin: a review","volume":"32","author":"Xiao","year":"2014","journal-title":"Biotechnol. Adv."}],"container-title":["Metabolic Engineering Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214030126000064?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214030126000064?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:50:58Z","timestamp":1781265058000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2214030126000064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":41,"alternative-id":["S2214030126000064"],"URL":"https:\/\/doi.org\/10.1016\/j.mec.2026.e00275","relation":{"has-preprint":[{"id-type":"doi","id":"10.64898\/2025.12.10.693358","asserted-by":"object"}]},"ISSN":["2214-0301"],"issn-type":[{"value":"2214-0301","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Metabolic engineering of Methanothermobacter thermautotrophicus \u0394H for recombinant acetoin production","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mec.2026.e00275","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V. on behalf of International Metabolic Engineering Society.","name":"copyright","label":"Copyright"}],"article-number":"e00275"}}