{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:52:30Z","timestamp":1786121550170,"version":"build-2736575974"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008223","name":"Universidade Federal do Paran\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008223","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011875","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia, Inova\u00e7\u00f5es e Comunica\u00e7\u00f5es","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011875","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006366","name":"Minist\u00e9rio da Educa\u00e7\u00e3o","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006366","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolic Engineering Communications"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.mec.2026.e00286","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:30:59Z","timestamp":1784647859000},"page":"e00286","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Reconstituting a two-step pathway for N,N-dimethyltryptamine (DMT) biosynthesis in bacteria"],"prefix":"10.1016","volume":"23","author":[{"given":"Lucas Henrique","family":"Junges","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavia Lada Degaut","family":"Pontes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Jos\u00e9 Teles","family":"Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Passaglia Bruschi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paula Fernandes","family":"Bonaldi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel Maltempi","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"M\u00fcller-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.mec.2026.e00286_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ymben.2026.102490","article-title":"Genome-based optimization of psilocybin and N,N-dimethyltryptamine biosynthetic pathways in E. coli using CRISPR-associated transposases","volume":"97","author":"Abrahms","year":"2026","journal-title":"Metab. Eng."},{"key":"10.1016\/j.mec.2026.e00286_bib2","doi-asserted-by":"crossref","DOI":"10.1016\/j.copbio.2025.103314","article-title":"Pathway engineering for the biosynthesis of psychedelics","volume":"94","author":"Abrahms","year":"2025","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.mec.2026.e00286_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.mec.2022.e00196","article-title":"Development of an E. Coli-based norbaeocystin production platform and evaluation of behavioral effects in rats","volume":"14","author":"Adams","year":"2022","journal-title":"Metab. Eng. Commun."},{"key":"10.1016\/j.mec.2026.e00286_bib4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.ymben.2019.09.009","article-title":"In vivo production of psilocybin in E. coli","volume":"56","author":"Adams","year":"2019","journal-title":"Metab. Eng."},{"key":"10.1016\/j.mec.2026.e00286_bib5","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1126\/science.134.3475.343","article-title":"Enzymatic formation of psychotomimetic metabolites from normally occurring compounds","volume":"134","author":"Axelrod","year":"1961","journal-title":"Science"},{"key":"10.1016\/j.mec.2026.e00286_bib6","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2018.00536","article-title":"N,N-dimethyltryptamine (DMT), an endogenous hallucinogen: past, present, and future research to determine its role and function","volume":"12","author":"Barker","year":"2018","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.mec.2026.e00286_bib7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.cbpa.2017.01.020","article-title":"Recent advances in methyltransferase biocatalysis","volume":"37","author":"Bennett","year":"2017","journal-title":"Curr. Opin. Chem. Biol."},{"key":"10.1016\/j.mec.2026.e00286_bib8","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aeb3034","article-title":"Complete biosynthesis of psychedelic tryptamines from three kingdoms in plants","volume":"12","author":"Berman","year":"2026","journal-title":"Sci. Adv."},{"key":"10.1016\/j.mec.2026.e00286_bib9","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1128\/jb.62.3.293-300.1951","article-title":"Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli","volume":"62","author":"Bertani","year":"1951","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mec.2026.e00286_bib10","doi-asserted-by":"crossref","first-page":"10028","DOI":"10.1002\/chem.201801047","article-title":"Biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions","volume":"24","author":"Blei","year":"2018","journal-title":"Chem. Eur J."},{"key":"10.1016\/j.mec.2026.e00286_bib11","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.mec.2026.e00286_bib12","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/s41386-022-01389-z","article-title":"Towards an understanding of psychedelic-induced neuroplasticity","volume":"48","author":"Calder","year":"2023","journal-title":"Neuropsychopharmacology"},{"key":"10.1016\/j.mec.2026.e00286_bib13","first-page":"1245","article-title":"Influence of environmental factors and cultural methods on the content of N,N-Dimethyltryptamine in Psychotria viridis (Rubiaceae)","volume":"29","author":"Cavalcante","year":"2018","journal-title":"J. Braz. Chem. Soc."},{"key":"10.1016\/j.mec.2026.e00286_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2023.105231","article-title":"A cane toad (Rhinella marina) N-methyltransferase converts primary indolethylamines to tertiary psychedelic amines","volume":"299","author":"Chen","year":"2023","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mec.2026.e00286_bib15","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1002\/cphc.201200793","article-title":"The effect of bacterial signal indole on the electrical properties of lipid membranes","volume":"14","author":"Chimerel","year":"2013","journal-title":"ChemPhysChem"},{"key":"10.1016\/j.mec.2026.e00286_bib16","doi-asserted-by":"crossref","first-page":"12524","DOI":"10.1002\/ange.201705489","article-title":"Enzymatische Synthese von Psilocybin","volume":"129","author":"Fricke","year":"2017","journal-title":"Angew. Chem."},{"key":"10.1016\/j.mec.2026.e00286_bib17","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ymben.2023.05.006","article-title":"In vivo biosynthesis of N,N-dimethyltryptamine, 5-MeO-N,N-dimethyltryptamine, and bufotenine in E. coli","volume":"78","author":"Friedberg","year":"2023","journal-title":"Metab. Eng."},{"key":"10.1016\/j.mec.2026.e00286_bib18","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0041680","article-title":"Pyridoxal 5\u2032-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase","volume":"7","author":"Ghatge","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.mec.2026.e00286_bib19","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1056\/NEJMoa2206443","article-title":"Single-dose psilocybin for a treatment-resistant episode of major depression","volume":"387","author":"Goodwin","year":"2022","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.mec.2026.e00286_bib20","series-title":"Molecular Cloning: a Laboratory Manual","author":"Green","year":"2012"},{"key":"10.1016\/j.mec.2026.e00286_bib21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.pharmthera.2004.03.003","article-title":"Hallucinogens and dissociative agents naturally growing in the United States","volume":"102","author":"Halpern","year":"2004","journal-title":"Pharmacol. Ther."},{"key":"10.1016\/j.mec.2026.e00286_bib22","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ymben.2014.11.003","article-title":"Production of chondroitin in metabolically engineered E. coli","volume":"27","author":"He","year":"2015","journal-title":"Metab. Eng."},{"key":"10.1016\/j.mec.2026.e00286_bib23","doi-asserted-by":"crossref","DOI":"10.1002\/btpr.3513","article-title":"Exploring the biocatalysis of psilocybin and other tryptamines: enzymatic pathways, synthetic strategies, and industrial implications","volume":"41","author":"Junges","year":"2025","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.mec.2026.e00286_bib24","doi-asserted-by":"crossref","DOI":"10.1002\/btpr.3492","article-title":"Evaluation of TrpM and PsiD substrate promiscuity reveals new biocatalytic capabilities","volume":"40","author":"Kanis","year":"2024","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.mec.2026.e00286_bib25","doi-asserted-by":"crossref","first-page":"5595","DOI":"10.1128\/jb.175.17.5595-5603.1993","article-title":"Isoleucine synthesis in Corynebacterium glutamicum: Molecular analysis of the ilvB-ilvN-ilvC operon","volume":"175","author":"Keilhauer","year":"1993","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mec.2026.e00286_bib26","doi-asserted-by":"crossref","DOI":"10.1002\/cbic.202200007","article-title":"Fermentative production of halogenated tryptophan derivatives with Corynebacterium glutamicum overexpressing tryptophanase or decarboxylase genes","volume":"23","author":"Kerbs","year":"2022","journal-title":"Chembiochem"},{"key":"10.1016\/j.mec.2026.e00286_bib27","doi-asserted-by":"crossref","DOI":"10.1038\/msb.2010.88","article-title":"Three serendipitous pathways in E. coli can bypass a block in pyridoxal-5\u2019-phosphate synthesis","volume":"6","author":"Kim","year":"2010","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.mec.2026.e00286_bib28","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1128\/JB.181.5.1530-1536.1999","article-title":"Role of ribosome release in regulation of tna operon expression in Escherichia coli","volume":"181","author":"Konan","year":"1999","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.mec.2026.e00286_bib29","doi-asserted-by":"crossref","DOI":"10.1186\/s12934-016-0459-x","article-title":"Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway","volume":"15","author":"Kunjapur","year":"2016","journal-title":"Microb. Cell Fact."},{"key":"10.1016\/j.mec.2026.e00286_bib30","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.mec.2026.e00286_bib31","doi-asserted-by":"crossref","first-page":"3170","DOI":"10.1016\/j.celrep.2018.05.022","article-title":"Psychedelics promote structural and functional neural plasticity","volume":"23","author":"Ly","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.mec.2026.e00286_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-022-32789-w","article-title":"Substrate multiplexed protein engineering facilitates promiscuous biocatalytic synthesis","volume":"13","author":"McDonald","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mec.2026.e00286_bib33","article-title":"Structural basis for psilocybin biosynthesis","volume":"16","author":"Meng","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.mec.2026.e00286_bib34","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.1038\/s41591-023-02565-4","article-title":"MDMA-assisted therapy for moderate to severe PTSD: a randomized, placebo-controlled phase 3 trial","volume":"29","author":"Mitchell","year":"2023","journal-title":"Nat. Med."},{"key":"10.1016\/j.mec.2026.e00286_bib35","doi-asserted-by":"crossref","DOI":"10.3390\/bioengineering11050412","article-title":"A novel bio-purification process employing an engineered E. coli strain for downstream processing of lactic acid solutions from the fermentation of agro-industrial by-Products","volume":"11","author":"Nastouli","year":"2024","journal-title":"Bioengineering"},{"key":"10.1016\/j.mec.2026.e00286_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/catal10091001","article-title":"Enhancement of S-adenosylmethionine-dependent methylation by integrating methanol metabolism with 5-methyl-tetrahydrofolate formation in Escherichia coli","volume":"10","author":"Okano","year":"2020","journal-title":"Catalysts"},{"key":"10.1016\/j.mec.2026.e00286_bib37","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.mec.2026.e00286_bib38","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.tibs.2023.12.009","article-title":"Unlocking the biosynthesis of psychedelic-inspired indolethylamines","volume":"49","author":"Sen","year":"2024","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.mec.2026.e00286_bib39","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1021\/acs.chemrev.7b00203","article-title":"Role of biocatalysis in sustainable chemistry","volume":"118","author":"Sheldon","year":"2018","journal-title":"Chem. Rev."},{"key":"10.1016\/j.mec.2026.e00286_bib40","doi-asserted-by":"crossref","first-page":"106","DOI":"10.3390\/medicines6040106","article-title":"Toxicological aspects and determination of the main components of ayahuasca: a critical review","volume":"6","author":"Sim\u00e3o","year":"2019","journal-title":"Medicines"},{"key":"10.1016\/j.mec.2026.e00286_bib41","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1039\/D4MD00562G","article-title":"Continuous flow synthesis of N,N-dimethyltryptamine (DMT) analogues with therapeutic potential","volume":"16","author":"Simoens","year":"2024","journal-title":"RSC Med. Chem."},{"key":"10.1016\/j.mec.2026.e00286_bib42","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0091-3057(98)00110-5","article-title":"Agonist properties of N,N-Dimethyltryptamine at serotonin 5-HT2A and 5-HT2C receptors","volume":"61","author":"Smith","year":"1998","journal-title":"Pharmacol. Biochem. Behav."},{"key":"10.1016\/j.mec.2026.e00286_bib43","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1002\/cbic.201200556","article-title":"S-Adenosyl-Methionine-Dependent methyltransferases: highly versatile enzymes in biocatalysis, biosynthesis and other biotechnological applications","volume":"13","author":"Struck","year":"2012","journal-title":"Chembiochem"},{"key":"10.1016\/j.mec.2026.e00286_bib44","doi-asserted-by":"crossref","first-page":"362","DOI":"10.2323\/jgam.2017.02.008","article-title":"Identification of YbhA as the pyridoxal 5\u2019-phosphate (PLP) phosphatase in Escherichia coli: importance of PLP homeostasis on the bacterial growth","volume":"63","author":"Sugimoto","year":"2017","journal-title":"J. Gen. Appl. Microbiol."},{"key":"10.1016\/j.mec.2026.e00286_bib45","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/0378-1119(88)90394-0","article-title":"New cloning vectors and techniques for easy and rapid restriction mapping","volume":"67","author":"Tartof","year":"1988","journal-title":"Gene"},{"key":"10.1016\/j.mec.2026.e00286_bib46","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/geno.1999.5960","article-title":"Human indolethylamine N-Methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization","volume":"61","author":"Thompson","year":"1999","journal-title":"Genomics"},{"key":"10.1016\/j.mec.2026.e00286_bib47","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.euroneuro.2023.07.003","article-title":"The potential of psychedelics for the treatment of Alzheimer's disease and related dementias","volume":"76","author":"Winkelman","year":"2023","journal-title":"Eur. Neuropsychopharmacol."},{"key":"10.1016\/j.mec.2026.e00286_bib48","doi-asserted-by":"crossref","DOI":"10.1186\/s40643-021-00425-y","article-title":"Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification","volume":"8","author":"Zhang","year":"2021","journal-title":"Bioresour. Bioprocess."}],"container-title":["Metabolic Engineering Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214030126000179?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2214030126000179?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T05:32:11Z","timestamp":1786080731000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2214030126000179"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":48,"alternative-id":["S2214030126000179"],"URL":"https:\/\/doi.org\/10.1016\/j.mec.2026.e00286","relation":{},"ISSN":["2214-0301"],"issn-type":[{"value":"2214-0301","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Reconstituting a two-step pathway for N,N-dimethyltryptamine (DMT) biosynthesis in bacteria","name":"articletitle","label":"Article Title"},{"value":"Metabolic Engineering Communications","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mec.2026.e00286","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V. on behalf of International Metabolic Engineering Society.","name":"copyright","label":"Copyright"}],"article-number":"e00286"}}