{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T07:30:17Z","timestamp":1778743817221,"version":"3.51.4"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Infection, Genetics and Evolution"],"published-print":{"date-parts":[[2013,10]]},"DOI":"10.1016\/j.meegid.2013.07.021","type":"journal-article","created":{"date-parts":[[2013,7,25]],"date-time":"2013-07-25T17:16:42Z","timestamp":1374772602000},"page":"226-231","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":52,"special_numbering":"C","title":["Genetic diversity of Leishmania major strains isolated from different clinical forms of cutaneous leishmaniasis in southern Iran based on minicircle kDNA"],"prefix":"10.1016","volume":"19","author":[{"given":"Ahmad","family":"Oryan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sadegh","family":"Shirian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad-Reza","family":"Tabandeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gholam-Reza","family":"Hatam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerrit","family":"Randau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahya","family":"Daneshbod","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.meegid.2013.07.021_b0005","first-page":"85","article-title":"Geographical distribution of leishmania species of human cutaneous leishmaniasis in fars province, southern Iran","volume":"8","author":"Akhoundi","year":"2013","journal-title":"Iran. J. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0010","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.meegid.2013.07.021_b0015","first-page":"7","article-title":"Zoonotic cutaneous leishmaniasis in Shiraz, Southern Iran","volume":"12","author":"Asgari","year":"2007","journal-title":"J. Res. Med. Sci."},{"key":"10.1016\/j.meegid.2013.07.021_b0020","first-page":"51","article-title":"Intraspecific variation in Leishmania major isolated from different forms of zoonotic Cutaneous Leishmaniasis","volume":"30","author":"Baghaei","year":"2005","journal-title":"IJMS"},{"key":"10.1016\/j.meegid.2013.07.021_b0025","doi-asserted-by":"crossref","first-page":"3391","DOI":"10.1128\/JCM.40.9.3391-3397.2002","article-title":"Practical approach for typing strains of Leishmania infantum by microsatellite analysis","volume":"40","author":"Bulle","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2013.07.021_b0030","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0035-9203(02)90052-0","article-title":"Analysis of minicircle classes in Leishmania (Viannia) species","volume":"96","author":"Brewster","year":"2002","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.meegid.2013.07.021_b0035","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.trstmh.2004.02.005","article-title":"Atypical cutaneous leishmaniasis in Central America: possible interaction between infectious and environmental elements","volume":"99","author":"Convit","year":"2005","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.meegid.2013.07.021_b0040","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1128\/JCM.41.7.3126-3132.2003","article-title":"Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil","volume":"41","author":"Cupolillo","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2013.07.021_b0045","first-page":"151","article-title":"Genetic polymorphism of Leishmania major in two hyper endemic regions of Iran revealed by PPIP-PCR and ITS- RFLP","volume":"15","author":"Dabirzadeh","year":"2012","journal-title":"Arch. Iran. Med."},{"key":"10.1016\/j.meegid.2013.07.021_b0050","doi-asserted-by":"crossref","first-page":"478","DOI":"10.5858\/2010-0069-OA.1","article-title":"Clinical, histopathologic, and cytologic diagnosis of mucosal leishmaniasis and literature review","volume":"135","author":"Daneshbod","year":"2011","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.meegid.2013.07.021_b0055","first-page":"CR530","article-title":"Comparative molecular epidemiology of Leishmania major and Leishmania tropica by PCR-RFLP technique in hyper endemic cities of Isfahan and Bam","volume":"16","author":"Doudi","year":"2010","journal-title":"Iran. Med. Sci. Monit."},{"key":"10.1016\/j.meegid.2013.07.021_b9005","first-page":"168","article-title":"Genetic analysis of clinical isolates of Leishmania major from Isfahan, Iran","volume":"49","author":"Eslami","year":"2012","journal-title":"J. Vector. Borne. Dis."},{"key":"10.1016\/j.meegid.2013.07.021_b0060","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1006\/expr.1995.1084","article-title":"Leishmania (Viannia) braziliensis: genetic relationships between strains isolated from different areas of Brazil as revealed by DNA fingerprinting and RAPD","volume":"80","author":"Gomes","year":"1995","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0065","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1111\/j.1468-3083.2008.02674.x","article-title":"Atypical presentation of old-world cutaneous leishmaniasis, diagnosis and species identification by PCR","volume":"22","author":"Karamian","year":"2008","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"10.1016\/j.meegid.2013.07.021_b0070","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.meegid.2004.05.008","article-title":"Sudanese mucosal leishmaniasis: isolation of a parasite within the Leishmania donovani complex that differs genotypically from L. donovani causing classical visceral leishmaniasis","volume":"5","author":"Mahdi","year":"2005","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.meegid.2013.07.021_b0075","doi-asserted-by":"crossref","first-page":"18","DOI":"10.5070\/D385P9D1ST","article-title":"Atypical cutaneous leishmaniasis resembling eczema on the foot","volume":"12","author":"Manzur","year":"2006","journal-title":"Dermatol. Online J."},{"key":"10.1016\/j.meegid.2013.07.021_b0080","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1179\/136485907X176445","article-title":"A search for the rodent hosts of Leishmania major in the Larestan region of southern Iran: demonstration of the parasite in Tatera indica and Gerbillus sp., by microscopy, culture and PCR","volume":"101","author":"Mehrabani","year":"2007","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0085","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1093\/oxfordjournals.molbev.a026289","article-title":"A simple method for classifying genes and a bootstrap test for classifications","volume":"17","author":"Misawa","year":"2000","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.meegid.2013.07.021_b0090","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1046\/j.1365-4362.2003.01730.x","article-title":"Disseminated mucocutaneous leishmaniasis resulting from chronic use of corticosteroid","volume":"42","author":"Motta","year":"2003","journal-title":"Int. J. Dermatol."},{"key":"10.1016\/j.meegid.2013.07.021_b0095","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1016\/S0140-6736(05)67629-5","article-title":"Advances in leishmaniasis","volume":"366","author":"Murray","year":"2005","journal-title":"Lancet"},{"key":"10.1016\/j.meegid.2013.07.021_b0100","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1128\/JCM.36.10.2877-2881.1998","article-title":"A nested PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan","volume":"36","author":"Noyes","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2013.07.021_b0105","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1007\/s00436-012-3240-z","article-title":"Molecular, cytological, and immunocytochemical study and kDNA sequencing of laryngeal Leishmania infantum infection","volume":"112","author":"Oryan","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.meegid.2013.07.021_b0110","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1179\/000349803225002589","article-title":"Leishmania and HIV co-infection: dermatological manifestations","volume":"97","author":"Puig","year":"2003","journal-title":"Ann. Trop. Med. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0115","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1086\/382049","article-title":"Antigen genes for molecular epidemiology of leishmaniasis: polymorphism of cysteine proteinase B and surface metalloprotease glycoprotein 63 in the Leishmania donovani complex","volume":"189","author":"Quispe Tintaya","year":"2004","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.meegid.2013.07.021_b0120","first-page":"45","article-title":"Spatial distribution and molecular identification of Leishmania species from endemic foci of South-eastern Iran","volume":"7","author":"Sharifi","year":"2012","journal-title":"Iran. J. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0125","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1159\/000337450","article-title":"Molecular diagnosis and species identification of mucosal leishmaniasis in Iran, and correlation with cytological findings","volume":"56","author":"Shirian","year":"2012","journal-title":"Acta Cytol."},{"key":"10.1016\/j.meegid.2013.07.021_b0130","doi-asserted-by":"crossref","first-page":"3805","DOI":"10.1128\/JCM.01469-12","article-title":"Mixed mucosal leishmaniasis infection caused by Leishmania tropica and Leishmania major and brief literature review","volume":"50","author":"Shirian","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2013.07.021_b0135","article-title":"Comparison of conventional, molecular and immunohitochemical methods in diagnosis of typical and atypical cutaneous leishmaniasis","volume":"137","author":"Shirian","year":"2013","journal-title":"Arch. Pathol. Lab. Med."},{"key":"10.1016\/j.meegid.2013.07.021_b0140","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1179\/2047773213Y.0000000098","article-title":"Three Leishmania\/L. species- L. infantum, L. major, L .tropica as causative agents for mucosal leishmaniasis in Iran","volume":"107","author":"Shirian","year":"2013","journal-title":"Pathog. Glob. Health."},{"key":"10.1016\/j.meegid.2013.07.021_b0145","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1128\/JCM.42.4.1739-1741.2004","article-title":"DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans","volume":"42","author":"Sreenivas","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2013.07.021_b0150","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.actatropica.2006.01.010","article-title":"Alimohammadian MH.Leishmania major: genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer","volume":"98","author":"Tashakori","year":"2006","journal-title":"Acta Trop."},{"key":"10.1016\/j.meegid.2013.07.021_b0155","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of practical multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.meegid.2013.07.021_b0160","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(92)90208-2","article-title":"Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites","volume":"51","author":"van Eys","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0165","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0031182098002789","article-title":"The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia","volume":"117","author":"Victoir","year":"1998","journal-title":"Parasitology"},{"key":"10.1016\/j.meegid.2013.07.021_b0170","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14411\/fp.2000.001","article-title":"Phylogenetic analysis of Trypanosomatina (Protozoa: Kinetoplastida) based on minicircle conserved regions","volume":"47","author":"Yurchenko","year":"2000","journal-title":"Folia Parasitol."},{"key":"10.1016\/j.meegid.2013.07.021_b0175","doi-asserted-by":"crossref","first-page":"613","DOI":"10.4269\/ajtmh.2004.70.613","article-title":"Genetic polymorphism within the leishmania donovani complex: correlation with geographic origin","volume":"70","author":"Zemanova","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."}],"container-title":["Infection, Genetics and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134813002876?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134813002876?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T02:48:59Z","timestamp":1646189339000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567134813002876"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":36,"alternative-id":["S1567134813002876"],"URL":"https:\/\/doi.org\/10.1016\/j.meegid.2013.07.021","relation":{},"ISSN":["1567-1348"],"issn-type":[{"value":"1567-1348","type":"print"}],"subject":[],"published":{"date-parts":[[2013,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genetic diversity of Leishmania major strains isolated from different clinical forms of cutaneous leishmaniasis in southern Iran based on minicircle kDNA","name":"articletitle","label":"Article Title"},{"value":"Infection, Genetics and Evolution","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.meegid.2013.07.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}