{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:57:51Z","timestamp":1777478271762,"version":"3.51.4"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Infection, Genetics and Evolution"],"published-print":{"date-parts":[[2015,1]]},"DOI":"10.1016\/j.meegid.2014.11.021","type":"journal-article","created":{"date-parts":[[2014,11,26]],"date-time":"2014-11-26T21:41:26Z","timestamp":1417038086000},"page":"189-195","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Molecular epidemiology of enterovirus B77 isolated from non polio acute flaccid paralytic patients in Pakistan during 2013"],"prefix":"10.1016","volume":"29","author":[{"given":"Mehar","family":"Angez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahzad","family":"Shaukat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rabaab","family":"Zahra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adnan","family":"Khurshid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salmaan","family":"Sharif","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Masroor","family":"Alam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syed Sohail Zahoor","family":"Zaidi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.meegid.2014.11.021_b0005","unstructured":"Aaslav, K.K., Bancroft, E., Coulombier, D., Croneld, C., Danielsson, N., Jain, R., Birgitta, Jong, D., Kreidl, P., Pastore, L., Celentano, Szegedi, E., Huitric, E., 2013. Suspected outbreak of poliomyelitis in Syria: risk of importation and spread of poliovirus in the EU Rapid Risk Assessment."},{"key":"10.1016\/j.meegid.2014.11.021_b0010","first-page":"46","article-title":"Acute disseminated encephalomyelitis associated with enteroviral infection","volume":"15","author":"Agin","year":"2010","journal-title":"Neurosciences (Riyadh)"},{"key":"10.1016\/j.meegid.2014.11.021_b0015","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.meegid.2014.11.021_b0020","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s11262-012-0776-z","article-title":"Genetic diversity and molecular characterization of enteroviruses from sewage-polluted urban and rural rivers in the Philippines","volume":"45","author":"Apostol","year":"2012","journal-title":"Virus Genes"},{"key":"10.1016\/j.meegid.2014.11.021_b0025","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.virusres.2003.11.006","article-title":"Isolation and identification of an enterovirus 77 recovered from a refugee child from Kosovo, and characterization of the complete virus genome","volume":"99","author":"Bailly","year":"2004","journal-title":"Virus Res."},{"key":"10.1016\/j.meegid.2014.11.021_b0030","first-page":"423","article-title":"Routine enterovirus diagnosis in a human rhabdomyosarcoma cell line","volume":"58","author":"Bell","year":"1980","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.meegid.2014.11.021_b0035","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1128\/JCM.06657-11","article-title":"Molecular characterization of human enteroviruses in the central african republic: uncovering wide diversity and identification of a new human enterovirus a71 genogroup","volume":"50","author":"Bessaud","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0040","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1128\/JCM.00313-08","article-title":"Eight years of experience with molecular identification of human enteroviruses","volume":"46","author":"Blomqvist","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0045","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1099\/vir.0.008540-0","article-title":"Resolving ambiguities in genetic typing of human enterovirus species C clinical isolates and identification of enterovirus 96, 99 and 102","volume":"90","author":"Brown","year":"2009","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0050","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1186\/1743-422X-7-236","article-title":"Echoviruses are a major cause of aseptic meningitis in infants and young children in Kuwait","volume":"7","author":"Dalwai","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.meegid.2014.11.021_b0055","first-page":"597","article-title":"Epidemiology and clinical characteristics of acute flaccid paralysis associated with non-polio enterovirus isolation: the experience in the Americas","volume":"73","author":"Dietz","year":"1995","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.meegid.2014.11.021_b0060","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1006\/viro.1999.9703","article-title":"Genetic recombination of poliovirus in vitro and in vivo: temperature-dependent alteration of crossover sites","volume":"258","author":"Duggal","year":"1999","journal-title":"Virology"},{"key":"10.1016\/j.meegid.2014.11.021_b0065","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"10.1016\/j.meegid.2014.11.021_b0070","unstructured":"GPEI, 2013. Polio in the Syrian Arab Republic. The Global Polio Eradication Initiative."},{"key":"10.1016\/j.meegid.2014.11.021_b0075","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1128\/JCM.41.5.2230-2232.2003","article-title":"Acute flaccid paralysis from echovirus type 33 infection","volume":"41","author":"Grimwood","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0080","first-page":"275","article-title":"The rapid generation of mutation data matrices from protein sequences","volume":"8","author":"Jones","year":"1992","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.meegid.2014.11.021_b0085","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/jmv.20825","article-title":"New enteroviruses, EV-93 and EV-94, associated with acute flaccid paralysis in the Democratic Republic of the Congo","volume":"79","author":"Junttila","year":"2007","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0090","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1146\/annurev.micro.58.030603.123625","article-title":"Vaccine-derived polioviruses and the endgame strategy for global polio eradication","volume":"59","author":"Kew","year":"2005","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0095","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1128\/JCM.00702-09","article-title":"Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolates by real-time reverse transcription-PCR using degenerate primers and probes containing deoxyinosine residues","volume":"47","author":"Kilpatrick","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0100","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/BF01731581","article-title":"A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences","volume":"16","author":"Kimura","year":"1980","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.meegid.2014.11.021_b0105","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1007\/s007050050640","article-title":"Characterization of echovirus 25 (ECV 25) in the VP1\/2A gene junction region. Brief report","volume":"144","author":"Kunkel","year":"1999","journal-title":"Arch. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0110","doi-asserted-by":"crossref","first-page":"e61650","DOI":"10.1371\/journal.pone.0061650","article-title":"Characterization of the non-polio enterovirus infections associated with acute flaccid paralysis in South-Western India","volume":"8","author":"Laxmivandana","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.meegid.2014.11.021_b0115","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.3201\/eid1611.100672","article-title":"Enterovirus 75 encephalitis in children, southern India","volume":"16","author":"Lewthwaite","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0120","series-title":"Enteroviruses: Polioviruses, Coxsackieviruses, Echoviruses, and Newer Enteroviruses","author":"Pallansch","year":"2001"},{"key":"10.1016\/j.meegid.2014.11.021_b0125","series-title":"Virology","first-page":"655","article-title":"Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses","author":"Melnick","year":"1996"},{"key":"10.1016\/j.meegid.2014.11.021_b0130","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/j.1440-1754.2004.00295.x","article-title":"Acute transverse myelitis caused by Coxsackie virus B5 infection","volume":"40","author":"Minami","year":"2004","journal-title":"J. Paediatr. Child Health"},{"key":"10.1016\/j.meegid.2014.11.021_b0135","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1128\/CMR.11.1.202","article-title":"Molecular typing of enteroviruses: current status and future requirements. The European Union Concerted Action on Virus Meningitis and Encephalitis","volume":"11","author":"Muir","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.meegid.2014.11.021_b0140","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1099\/0022-1317-81-3-803","article-title":"Molecular epidemiology of coxsackievirus B4 and disclosure of the correct VP1\/2A(pro) cleavage site: evidence for high genomic diversity and long-term endemicity of distinct genotypes","volume":"81","author":"Mulders","year":"2000","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0145","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1099\/vir.0.18647-0","article-title":"Sequencing of \u2018untypable\u2019 enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types","volume":"84","author":"Norder","year":"2003","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0150","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1128\/JCM.37.5.1288-1293.1999","article-title":"Typing of human enteroviruses by partial sequencing of VP1","volume":"37","author":"Oberste","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2014.11.021_b0155","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1128\/JVI.73.3.1941-1948.1999","article-title":"Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification","volume":"73","author":"Oberste","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0160","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.virusres.2007.04.001","article-title":"Molecular identification of 13 new enterovirus types, EV79-88, EV97, and EV100-101, members of the species Human Enterovirus B","volume":"128","author":"Oberste","year":"2007","journal-title":"Virus Res."},{"key":"10.1016\/j.meegid.2014.11.021_b0165","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1099\/vir.0.81179-0","article-title":"Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses","volume":"87","author":"Oberste","year":"2006","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0170","doi-asserted-by":"crossref","first-page":"3205","DOI":"10.1099\/vir.0.80148-0","article-title":"Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75","volume":"85","author":"Oberste","year":"2004","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0175","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.3201\/eid1811.111457","article-title":"Antigenic diversity of enteroviruses associated with nonpolio acute flaccid paralysis, India, 2007\u20132009","volume":"18","author":"Rao","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0180","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.meegid.2013.04.011","article-title":"Non-polio enteroviruses and their association with acute diarrhea in children in India","volume":"17","author":"Raoa","year":"2013","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.meegid.2014.11.021_b0185","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0042-6822(87)90001-8","article-title":"Geographic distribution of wild poliovirus type 1 genotypes","volume":"160","author":"Rico-Hesse","year":"1987","journal-title":"Virology"},{"key":"10.1016\/j.meegid.2014.11.021_b0190","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1093\/clinids\/20.4.971","article-title":"Enteroviral infections of the central nervous system","volume":"20","author":"Rotbart","year":"1995","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0195","doi-asserted-by":"crossref","first-page":"e3052","DOI":"10.1371\/journal.pntd.0003052","article-title":"Characterization of Enteroviruses from non-human primates in cameroon revealed virus types widespread in humans along with candidate new types and species","volume":"8","author":"Sadeuh-Mba","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0200","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1471-2334-7-6","article-title":"Epidemiology and clinical findings associated with enteroviral acute flaccid paralysis in Pakistan","volume":"7","author":"Saeed","year":"2007","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0205","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.meegid.2014.11.021_b0210","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1099\/0022-1317-81-5-1361","article-title":"Molecular epidemiology and evolution of coxsackievirus A9","volume":"81","author":"Santti","year":"2000","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0215","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1186\/1743-422X-11-146","article-title":"Identification and characterization of unrecognized viruses in stool samples of non-polio acute flaccid paralysis children by simplified VIDISCA","volume":"11","author":"Shaukat","year":"2014","journal-title":"Virol. J."},{"key":"10.1016\/j.meegid.2014.11.021_b0220","doi-asserted-by":"crossref","first-page":"e80040","DOI":"10.1371\/journal.pone.0080040","article-title":"Characterization of a novel enterovirus serotype and an enterovirus EV-B93 isolated from acute flaccid paralysis patients","volume":"8","author":"Shaukat","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.meegid.2014.11.021_b0225","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1097\/00001432-200310000-00002","article-title":"Infectious causes of acute flaccid paralysis","volume":"16","author":"Solomon","year":"2003","journal-title":"Curr. Opin. Infect. Dis."},{"key":"10.1016\/j.meegid.2014.11.021_b0230","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.meegid.2014.11.021_b0235","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.meegid.2014.11.021_b0240","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1017\/S0950268899003726","article-title":"Enteroviruses in Spain: virological and epidemiological studies over 10 years (1988\u201397)","volume":"124","author":"Trallero","year":"2000","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.meegid.2014.11.021_b0245","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1128\/JVI.1.3.478-488.1967","article-title":"Virus aggregation as the cause of the non-neutralizable persistent fraction","volume":"1","author":"Wallis","year":"1967","journal-title":"J. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0250","series-title":"Polio Laboratory Manual","year":"2004"},{"key":"10.1016\/j.meegid.2014.11.021_b0255","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1002\/(SICI)1096-9071(199906)58:2<188::AID-JMV15>3.0.CO;2-H","article-title":"L20B cells simplify culture of polioviruses from clinical samples","volume":"58","author":"Wood","year":"1999","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.meegid.2014.11.021_b0260","first-page":"38","article-title":"Coxsackieviruses infection in northern Taiwan-epidemiology and clinical characteristics","volume":"42","author":"Yen","year":"2009","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.meegid.2014.11.021_b0265","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1186\/1743-422X-10-222","article-title":"Genetic characterization of EV71 isolates from 2004 to 2010 reveals predominance and persistent circulation of the newly proposed genotype D and recent emergence of a distinct lineage of subgenotype C2 in Hong Kong","volume":"10","author":"Yip","year":"2013","journal-title":"Virol. J."}],"container-title":["Infection, Genetics and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134814004389?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134814004389?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T07:34:10Z","timestamp":1598513650000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567134814004389"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":53,"alternative-id":["S1567134814004389"],"URL":"https:\/\/doi.org\/10.1016\/j.meegid.2014.11.021","relation":{},"ISSN":["1567-1348"],"issn-type":[{"value":"1567-1348","type":"print"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular epidemiology of enterovirus B77 isolated from non polio acute flaccid paralytic patients in Pakistan during 2013","name":"articletitle","label":"Article Title"},{"value":"Infection, Genetics and Evolution","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.meegid.2014.11.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}