{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:49:03Z","timestamp":1783010943807,"version":"3.54.6"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Infection, Genetics and Evolution"],"published-print":{"date-parts":[[2015,4]]},"DOI":"10.1016\/j.meegid.2015.01.023","type":"journal-article","created":{"date-parts":[[2015,2,7]],"date-time":"2015-02-07T13:51:19Z","timestamp":1423317079000},"page":"190-197","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Multilocus typing and population structure of Cryptosporidium from children in Zaragoza, Spain"],"prefix":"10.1016","volume":"31","author":[{"given":"Ana","family":"Ramo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaqu\u00edn","family":"Qu\u00edlez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Vergara-Castiblanco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Monteagudo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Del Cacho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Clavel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.meegid.2015.01.023_b0005","doi-asserted-by":"crossref","first-page":"2744","DOI":"10.1128\/JCM.41.6.2744-2747.2003","article-title":"Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal","volume":"41","author":"Alves","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0010","doi-asserted-by":"crossref","first-page":"20070","DOI":"10.2807\/ese.17.05.20070-en","article-title":"Outbreak of cryptosporidiosis in a child day-care centre in Gipuzkoa, Spain, October to December 2011","volume":"17","author":"Artieda","year":"2012","journal-title":"Euro Surveill."},{"key":"10.1016\/j.meegid.2015.01.023_b0015","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1128\/CMR.00076-12","article-title":"Cryptosporidium pathogenicity and virulence","volume":"26","author":"Bouzid","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.meegid.2015.01.023_b0020","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1017\/S0031182099005508","article-title":"A microsatellite marker reveals population heterogeneity within humans and animal genotypes of Cryptosporidium parvum","volume":"120","author":"Cacci\u00f2","year":"2000","journal-title":"Parasitology"},{"key":"10.1016\/j.meegid.2015.01.023_b0025","doi-asserted-by":"crossref","first-page":"496","DOI":"10.3201\/eid1403.071320","article-title":"Geographic linkage and variation in Cryptosporidium hominis","volume":"14","author":"Chalmers","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0030","doi-asserted-by":"crossref","first-page":"536","DOI":"10.3201\/eid1703.100410","article-title":"Sporadic human cryptosporidiosis caused by Cryptosporidium cuniculus, United Kingdom, 2007\u20132008","volume":"17","author":"Chalmers","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0035","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.pt.2013.03.001","article-title":"Looking for Cryptosporidium: the application of advances in detection and diagnosis","volume":"29","author":"Chalmers","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0040","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.eimc.2012.04.011","article-title":"Two cases of zoonotic cryptosporidiosis in Spain by the unusual species Cryptosporidium ubiquitum and Cryptosporidium felis","volume":"30","author":"Cieloszyk","year":"2012","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"10.1016\/j.meegid.2015.01.023_b0045","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/BF01586190","article-title":"Seasonality of cryptosporidiosis in children","volume":"15","author":"Clavel","year":"1996","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0050","doi-asserted-by":"crossref","first-page":"855","DOI":"10.2307\/2410189","article-title":"Estimation of gene flow from F-statistics","volume":"47","author":"Cockerham","year":"1993","journal-title":"Evolution"},{"key":"10.1016\/j.meegid.2015.01.023_b0055","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.vetpar.2011.11.039","article-title":"Assessment of three methods for multilocus fragment typing of Cryptosporidium parvum from domestic ruminants in North West Spain","volume":"186","author":"D\u00edaz","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0060","doi-asserted-by":"crossref","first-page":"5581","DOI":"10.1128\/AEM.67.12.5581-5584.2001","article-title":"The modification of a rapid method for the identification of gene-specific polymorphisms in Cryptosporidium parvum, and its application to clinical and epidemiological investigations","volume":"67","author":"Elwin","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0065","doi-asserted-by":"crossref","first-page":"4303","DOI":"10.1128\/JCM.01067-06","article-title":"Evidence supporting zoonotic transmission of Cryptosporidium in Wisconsin","volume":"44","author":"Feltus","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0070","doi-asserted-by":"crossref","first-page":"312","DOI":"10.3201\/eid1802.110666","article-title":"Extended outbreak of cryptosporidiosis in a pediatric hospital, China","volume":"18","author":"Feng","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0075","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1016\/j.ijpara.2013.09.002","article-title":"Population genetic characterisation of dominant Cryptosporidium parvum subtype IIaA15G2R1","volume":"43","author":"Feng","year":"2013","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0080","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1128\/JCM.02973-13","article-title":"Multilocus sequence typing of an emerging Cryptosporidium hominis subtype in the United States","volume":"52","author":"Feng","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0085","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1471-2105-13-87","article-title":"PHYLOViZ: phylogenetic inference and data visualization for sequence based typing methods","volume":"13","author":"Francisco","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.meegid.2015.01.023_b0090","article-title":"Cryptosporidium hominis genotypes involved in increased incidence and clusters of cases, Navarra, Spain","author":"Fuentes","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"10.1016\/j.meegid.2015.01.023_b0095","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1002\/jez.b.21026","article-title":"Inferring species phylogenies from multiple genes: concatenated sequence tree versus consensus gene tree","volume":"304","author":"Gadagkar","year":"2005","journal-title":"J. Exp. Zool. B Mol. Dev. Evol."},{"key":"10.1016\/j.meegid.2015.01.023_b0100","first-page":"2275","article-title":"Cryptosporidiosis outbreak in British tourists who stayed at a hotel in Majorca, Spain","volume":"7","author":"Galmes","year":"2003","journal-title":"Euro Surveill."},{"key":"10.1016\/j.meegid.2015.01.023_b0105","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.meegid.2006.08.006","article-title":"Multilocus sequence typing and genetic structure of Cryptosporidium hominis from children in Kolkata, India","volume":"7","author":"Gatei","year":"2007","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.meegid.2015.01.023_b0110","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1093\/bioinformatics\/16.9.847","article-title":"LIAN 3.0: detecting linkage disequilibrium in multilocus data","volume":"16","author":"Haubold","year":"2000","journal-title":"Bioinformatics"},{"key":"10.1016\/j.meegid.2015.01.023_b0115","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.vetpar.2012.12.015","article-title":"Molecular epidemiology of Cryptosporidium in livestock animals and humans in the Ismailia province of Egypt","volume":"193","author":"Helmy","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0120","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/JCM.26.11.2465-2466.1988","article-title":"Numerical index of the discriminatory ability of typing systems: an application of Simpson\u2019s index of diversity","volume":"26","author":"Hunter","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0125","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.biotechadv.2009.08.003","article-title":"Genetic richness and diversity in Cryptosporidium hominis and C. parvum reveals major knowledge gaps and a need for the application of \u201cnext generation\u201d technologies \u2013 research review","volume":"28","author":"Jex","year":"2010","journal-title":"Biotechnol. Adv."},{"key":"10.1016\/j.meegid.2015.01.023_b0130","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1128\/JCM.02536-06","article-title":"Multilocus analysis of Cryptosporidium hominis and Cryptosporidium parvum isolates from sporadic and outbreak-related human cases and C. parvum isolates from sporadic livestock cases in the United Kingdom","volume":"45","author":"Leoni","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0135","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.3201\/eid1910.121361","article-title":"Genetic recombination and Cryptosporidium hominis virulent subtype IbA10G2","volume":"19","author":"Li","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0140","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3201\/eid2002.121797","article-title":"Subtyping Cryptosporidium ubiquitum, a zoonotic pathogen emerging in humans","volume":"20","author":"Li","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.meegid.2015.01.023_b0145","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.parint.2007.02.003","article-title":"Genetic characterization of Cryptosporidium species from humans in Spain","volume":"56","author":"Llorente","year":"2007","journal-title":"Parasitol. Int."},{"key":"10.1016\/j.meegid.2015.01.023_b0150","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s15010-011-0188-3","article-title":"Under-notification of cryptosporidiosis by routine clinical and laboratory practices among non-hospitalised children with acute diarrhoea in Southern Spain","volume":"40","author":"Mart\u00edn-Ampudia","year":"2012","journal-title":"Infection"},{"issue":"Suppl. 1","key":"10.1016\/j.meegid.2015.01.023_b0155","doi-asserted-by":"crossref","first-page":"S33","DOI":"10.1111\/j.1550-7408.2006.00166.x","article-title":"Cryptosporidium population genetics: evidence of clonality in isolates from France and Haiti","volume":"53","author":"Ngouanesavanh","year":"2006","journal-title":"J. Eukaryot. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0160","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1002\/elps.200900706","article-title":"Highly sensitive non-isotopic restriction endonuclease fingerprinting of nucleotide variability in the gp60 gene within Cryptosporidium species, genotypes and subgenotypes infective to humans, and its implications","volume":"31","author":"Pangasa","year":"2010","journal-title":"Electrophoresis"},{"key":"10.1016\/j.meegid.2015.01.023_b0165","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1863-2378.2009.01247.x","article-title":"Spatial and temporal epidemiology of sporadic human cryptosporidiosis in Scotland","volume":"57","author":"Pollock","year":"2010","journal-title":"Zoonoses Public Health"},{"key":"10.1016\/j.meegid.2015.01.023_b0170","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/genetics\/155.2.945","article-title":"Inference of population structure using multilocus genotype data","volume":"155","author":"Pritchard","year":"2000","journal-title":"Genetics"},{"key":"10.1016\/j.meegid.2015.01.023_b0175","doi-asserted-by":"crossref","first-page":"7779","DOI":"10.1128\/AEM.00751-11","article-title":"Multilocus fragment typing and genetic structure of Cryptosporidium parvum isolates from diarrheic preweaned calves in Spain","volume":"77","author":"Qu\u00edlez","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0180","doi-asserted-by":"crossref","first-page":"5363","DOI":"10.1128\/AEM.01168-13","article-title":"Host association of Cryptosporidium parvum populations infecting domestic ruminants in Spain","volume":"79","author":"Qu\u00edlez","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0185","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1007\/s00436-014-3828-6","article-title":"Validation of fragment analysis by capillary electrophoresis to resolve mixed infections by Cryptosporidium parvum subpopulations","volume":"113","author":"Qu\u00edlez","year":"2014","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.meegid.2015.01.023_b0190","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.exppara.2012.06.016","article-title":"Assessment of polymorphic genetic markers for multi-locus typing of Cryptosporidium parvum and Cryptosporidium hominis","volume":"132","author":"Robinson","year":"2012","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0195","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1017\/S0031182008004800","article-title":"Genetic classification of Cryptosporidium isolates from humans and calves in Slovenia","volume":"135","author":"Soba","year":"2008","journal-title":"Parasitology"},{"key":"10.1016\/j.meegid.2015.01.023_b0200","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1128\/JCM.43.6.2805-2809.2005","article-title":"Unique endemicity of cryptosporidiosis in children in Kuwait","volume":"43","author":"Sulaiman","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0205","first-page":"569","article-title":"TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment","volume":"10","author":"Van de Peer","year":"1994","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.meegid.2015.01.023_b0210","doi-asserted-by":"crossref","first-page":"7757","DOI":"10.1128\/AEM.00615-11","article-title":"Molecular epidemiology, spatiotemporal analysis, and ecology of sporadic human cryptosporidiosis in Australia","volume":"77","author":"Waldron","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0215","doi-asserted-by":"crossref","first-page":"6639","DOI":"10.1128\/AEM.01268-10","article-title":"Comparison of single- and multilocus genetic diversity in the protozoan parasites Cryptosporidium parvum and C. hominis","volume":"76","author":"Widmer","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0220","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.ijpara.2007.10.014","article-title":"Molecular epidemiology of Cryptosporidium in human and cattle in the Netherlands","volume":"38","author":"Wielinga","year":"2008","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0225","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1128\/AEM.67.3.1097-1101.2001","article-title":"Molecular characterization of Cryptosporidium oocysts in samples of raw surface water and wastewater","volume":"67","author":"Xiao","year":"2001","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.meegid.2015.01.023_b0230","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.exppara.2009.03.018","article-title":"Molecular epidemiology of cryptosporidiosis: an update","volume":"124","author":"Xiao","year":"2010","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.meegid.2015.01.023_b0235","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1017\/S0950268808000769","article-title":"The prevalence of Cryptosporidium species and subtypes in human faecal samples in Ireland","volume":"137","author":"Zintl","year":"2009","journal-title":"Epidemiol. Infect."}],"container-title":["Infection, Genetics and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134815000362?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134815000362?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T21:03:16Z","timestamp":1651179796000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567134815000362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":47,"alternative-id":["S1567134815000362"],"URL":"https:\/\/doi.org\/10.1016\/j.meegid.2015.01.023","relation":{},"ISSN":["1567-1348"],"issn-type":[{"value":"1567-1348","type":"print"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Multilocus typing and population structure of Cryptosporidium from children in Zaragoza, Spain","name":"articletitle","label":"Article Title"},{"value":"Infection, Genetics and Evolution","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.meegid.2015.01.023","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}