{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:35:31Z","timestamp":1787970931322,"version":"build-2784847793"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Infection, Genetics and Evolution"],"published-print":{"date-parts":[[2017,10]]},"DOI":"10.1016\/j.meegid.2017.06.031","type":"journal-article","created":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T02:32:37Z","timestamp":1498876357000},"page":"170-175","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina"],"prefix":"10.1016","volume":"54","author":[{"given":"Rodolfo D.","family":"Casero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Mongi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Laconte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Rivero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Sastre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u00edbal","family":"Teher\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanny","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1344-9312","authenticated-orcid":false,"given":"Juan David","family":"Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.meegid.2017.06.031_bb0005","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1128\/JCM.43.4.1689-1693.2005","article-title":"Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infections","volume":"43","author":"Booton","year":"2005","journal-title":"J. Clin. Microbiol."},{"issue":"6","key":"10.1016\/j.meegid.2017.06.031_bb0010","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1097\/ICO.0b013e31819342a7","article-title":"Genotypic identification of Acanthamoeba sp. isolates associated with an outbreak of Acanthamoeba keratitis","volume":"28","author":"Booton","year":"2009","journal-title":"Cornea"},{"key":"10.1016\/j.meegid.2017.06.031_bb0015","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1097\/ICO.0b013e31826429bd","article-title":"Prognostic factors associated with the need for surgery in Acanthamoeba keratitis","volume":"32","author":"Bouheraoua","year":"2013","journal-title":"Cornea"},{"issue":"2","key":"10.1016\/j.meegid.2017.06.031_bb0020","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/opo.12271","article-title":"Strategies for the prevention of contact lens-related Acanthamoeba keratitis: a review","volume":"36","author":"Carnt","year":"2016","journal-title":"Ophthalmic Physiol. Opt."},{"issue":"5","key":"10.1016\/j.meegid.2017.06.031_bb0025","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1097\/ICO.0b013e318193e0fe","article-title":"Twenty years of Acanthamoeba keratitis","volume":"28","author":"Carvalho","year":"2009","journal-title":"Cornea"},{"key":"10.1016\/j.meegid.2017.06.031_bb0030","doi-asserted-by":"crossref","first-page":"484892","DOI":"10.1155\/2012\/484892","article-title":"Advances in the diagnosis and treatment of Acanthamoeba keratitis","volume":"2012","author":"Clarke","year":"2012","journal-title":"J. Ophthalmol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0035","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s00436-010-1870-6","article-title":"Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida)","volume":"107","author":"Corsaro","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.meegid.2017.06.031_bb0040","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/j.1550-7408.2009.00453.x","article-title":"Characterization of Acanthamoeba isolates from dust of a public hospital in Curitiba, Paran\u00e1, Brazil","volume":"57","author":"Costa","year":"2010","journal-title":"J. Eukaryot. Microbiol."},{"issue":"487\u201399","key":"10.1016\/j.meegid.2017.06.031_bb0045","article-title":"Acanthamoeba keratitis: diagnosis and treatment update 2009","volume":"148","author":"Dart","year":"2009","journal-title":"Am J. Ophthalmol."},{"issue":"1","key":"10.1016\/j.meegid.2017.06.031_bb0050","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.exppara.2013.05.013","article-title":"Morphological, genotypic, and physiological characterization of Acanthamoeba isolates from keratitis patients and the domestic environment in Vitoria, Esp\u00edrito Santo, Brazil","volume":"135","author":"Duarte","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0055","series-title":"Clinical Microbiology Procedures Handbook","year":"2010"},{"key":"10.1016\/j.meegid.2017.06.031_bb0060","first-page":"122","article-title":"Costamagna Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificaci\u00f3n molecular en Bah\u00eda Blanca, provincia de Buenos Aires, Argentina","volume":"42","author":"Gertiser","year":"2010","journal-title":"Rev. Arg. Microbiol."},{"issue":"2","key":"10.1016\/j.meegid.2017.06.031_bb0065","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1080\/09286580600920281","article-title":"Factors affecting the epidemiology of Acanthamoeba keratitis","volume":"14","author":"Ibrahim","year":"2007","journal-title":"Ophthalmic Epidemiol."},{"issue":"2","key":"10.1016\/j.meegid.2017.06.031_bb0070","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jtos.2015.01.002","article-title":"Acanthamoeba keratitis: clinical characteristics and management","volume":"13","author":"Jiang","year":"2015","journal-title":"Ocul. Surf."},{"key":"10.1016\/j.meegid.2017.06.031_bb0075","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1574-6976.2006.00023.x","article-title":"Acanthamoeba: biology and increasing importance in human health","volume":"30","author":"Khan","year":"2006","journal-title":"FEMS Microbiol. Rev."},{"issue":"7","key":"10.1016\/j.meegid.2017.06.031_bb0080","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"issue":"4","key":"10.1016\/j.meegid.2017.06.031_bb0085","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.pt.2013.01.006","article-title":"Acanthamoeba keratitis: an emerging disease gathering importance worldwide?","volume":"29","author":"Lorenzo-Morales","year":"2013","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0090","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1051\/parasite\/2015010","article-title":"An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment","volume":"22","author":"Lorenzo-Morales","year":"2015","journal-title":"Parasite"},{"issue":"3","key":"10.1016\/j.meegid.2017.06.031_bb0095","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.exppara.2009.07.006","article-title":"Genotyping, physiological features and proteolytic activities of a potentially pathogenic Acanthamoeba sp. isolated from tap water in Brazil","volume":"123","author":"Magliano","year":"2009","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0100","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s00436-011-2530-1","article-title":"Isolation of Acanthamoeba species in surface waters of Gilan province-north of Iran","volume":"110","author":"Mahmoudi","year":"2012","journal-title":"Parasitol. Res."},{"issue":"10","key":"10.1016\/j.meegid.2017.06.031_bb0105","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1128\/IAI.00345-16","article-title":"Acanthamoeba castellanii (genotype T4) stimulates the production of interleukin-10 as well as pro-inflammatory cytokines in THP-1 cells, human peripheral blood mononuclear cells and human monocyte-derived macrophages","volume":"84","author":"Mattana","year":"2016","journal-title":"Infect. Immun."},{"key":"10.1016\/j.meegid.2017.06.031_bb0110","first-page":"276","article-title":"Acanthamoeba sp.: un caso de queratitis no relacionada con el uso de lentes de contacto","volume":"44","author":"Menghi","year":"2012","journal-title":"Rev. Arg. Microbiol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0115","doi-asserted-by":"crossref","first-page":"4636","DOI":"10.1128\/JCM.01090-10","article-title":"Identification of a novel t17 genotype of Acanthamoeba from environmental isolates and t10 genotype causing keratitis in Thailand","volume":"48","author":"Nuprasert","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0120","series-title":"An Illustrated Key to Freshwater and Soil Amoebae: With Notes on Cultivation and Ecology","first-page":"155","author":"Page","year":"1976"},{"key":"10.1016\/j.meegid.2017.06.031_bb0125","doi-asserted-by":"crossref","first-page":"670242","DOI":"10.1155\/2013\/670242","article-title":"Acanthamoeba keratitis: a 12-year experience covering a wide spectrum of presentations, diagnoses, and outcomes","volume":"2013","author":"Page","year":"2013","journal-title":"J. Ophthalmol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0130","first-page":"557","article-title":"Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba (Protozoa, Amoebida)","volume":"8","author":"Pussard","year":"1977","journal-title":"Protistol"},{"issue":"5","key":"10.1016\/j.meegid.2017.06.031_bb0135","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1136\/bjo.86.5.536","article-title":"Acanthamoeba keratitis in England and Wales: incidence, outcome, and risk factors","volume":"86","author":"Radford","year":"2002","journal-title":"Br. J. Ophthalmol."},{"issue":"11","key":"10.1016\/j.meegid.2017.06.031_bb0140","doi-asserted-by":"crossref","first-page":"3807","DOI":"10.1007\/s00436-013-3572-3","article-title":"Genotyping and phylogenetic analysis of Acanthamoeba isolates associated with keratitis","volume":"112","author":"Risler","year":"2013","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.meegid.2017.06.031_bb0145","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1128\/JCM.39.5.1903-1911.2001","article-title":"Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoeba from humans with keratitis and from sewage sludge","volume":"39","author":"Schroeder","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.meegid.2017.06.031_bb0150","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1756-3305-5-6","article-title":"Biology and pathogenesis of Acanthamoeba","volume":"5","author":"Siddiqui","year":"2012","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.meegid.2017.06.031_bb0155","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1550-7408.1998.tb05068.x","article-title":"The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rDNA gene sequence types","volume":"45","author":"Stothard","year":"1998","journal-title":"J. Eukariot. Microbiol."},{"issue":"6","key":"10.1016\/j.meegid.2017.06.031_bb0160","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1097\/QCO.0b013e32833ed78b","article-title":"Amebic meningoencephalitides and keratitis: challenges in diagnosis and treatment","volume":"23","author":"Visvesvara","year":"2010","journal-title":"Curr. Opin. Infect. Dis."},{"issue":"10","key":"10.1016\/j.meegid.2017.06.031_bb0165","doi-asserted-by":"crossref","first-page":"4408","DOI":"10.1128\/AEM.66.10.4408-4413.2000","article-title":"Correlations between morphological, molecular biological and physiological characteristics in clinical and nonclinical isolates of Acanthamoeba spp.","volume":"66","author":"Walochnik","year":"2000","journal-title":"App. Environ. Microbiol."},{"issue":"1","key":"10.1016\/j.meegid.2017.06.031_bb0170","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/jeu.12149","article-title":"Twenty years of Acanthamoeba diagnostics in Austria","volume":"62","author":"Walochnik","year":"2015","journal-title":"J. Eukaryot. Microbiol."}],"container-title":["Infection, Genetics and Evolution"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134817302290?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1567134817302290?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T20:37:08Z","timestamp":1538685428000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1567134817302290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":34,"alternative-id":["S1567134817302290"],"URL":"https:\/\/doi.org\/10.1016\/j.meegid.2017.06.031","relation":{},"ISSN":["1567-1348"],"issn-type":[{"value":"1567-1348","type":"print"}],"subject":[],"published":{"date-parts":[[2017,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular and morphological characterization of Acanthamoeba isolated from corneal scrapes and contact lens wearers in Argentina","name":"articletitle","label":"Article Title"},{"value":"Infection, Genetics and Evolution","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.meegid.2017.06.031","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}