{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T19:28:26Z","timestamp":1750102106353,"version":"3.40.5"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Medical Hypotheses"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.mehy.2018.04.012","type":"journal-article","created":{"date-parts":[[2018,4,15]],"date-time":"2018-04-15T05:33:13Z","timestamp":1523770393000},"page":"1-3","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Blood cell disruption to significantly improve the Borrelia PCR detection sensitivity in borreliosis in humans"],"prefix":"10.1016","volume":"116","author":[{"given":"Alexis","family":"Lacout","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yves","family":"Mone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Franck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre-Yves","family":"Marcy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Mas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7893-7974","authenticated-orcid":false,"given":"Francisco","family":"Veas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Perronne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mehy.2018.04.012_b0005","doi-asserted-by":"crossref","first-page":"249","DOI":"10.2147\/IJGM.S140224","article-title":"Empirical validation of the Horowitz multiple systemic infectious disease syndrome questionnaire for suspected lyme disease","volume":"10","author":"Citera","year":"2017","journal-title":"Int J Gen Med"},{"key":"10.1016\/j.mehy.2018.04.012_b0010","doi-asserted-by":"crossref","first-page":"e219","DOI":"10.1016\/j.amjmed.2016.12.001","article-title":"Errancy on Lyme diagnosis","volume":"130","author":"Perronne","year":"2017","journal-title":"Am J Med"},{"key":"10.1016\/j.mehy.2018.04.012_b0015","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.idc.2007.12.005","article-title":"Lyme disease: laboratory issues","volume":"22","author":"Aguero-Rosenfeld","year":"2008","journal-title":"Infect Dis Clin North Am"},{"key":"10.1016\/j.mehy.2018.04.012_b0020","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1099\/jmm.0.45853-0","article-title":"Comparative evaluation of three different ELISA methods for the diagnosis of early culture-confirmed Lyme disease in Italy","volume":"54","author":"Marangoni","year":"2005","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.mehy.2018.04.012_b0025","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1007\/s10096-011-1157-6","article-title":"Large differences between test strategies for the detection of anti-Borrelia antibodies are revealed by comparing eight ELISAs and five immunoblots","volume":"30","author":"Ang","year":"2011","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.mehy.2018.04.012_b0030","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s00005-010-0111-0","article-title":"Serodiagnosis of borreliosis: indirect immunofluorescence assay, enzyme-linked immunosorbent assay and immunoblotting","volume":"59","author":"Wojciechowska-Koszko","year":"2011","journal-title":"Arch Immunol Ther Exp (Warsz)"},{"key":"10.1016\/j.mehy.2018.04.012_b0035","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1186\/s12879-016-1468-4","article-title":"The diagnostic accuracy of serological tests for Lyme borreliosis in Europe: a systematic review and meta-analysis","volume":"16","author":"Leeflang","year":"2016","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.mehy.2018.04.012_b0040","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/1742-2094-5-40","article-title":"Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme neuroborreliosis","volume":"5","author":"Miklossy","year":"2008","journal-title":"J Neuroinflamm"},{"key":"10.1016\/j.mehy.2018.04.012_b0045","doi-asserted-by":"crossref","first-page":"97","DOI":"10.2147\/IDR.S19201","article-title":"Evaluation of in-vitro antibiotic susceptibility of different morphological forms of Borrelia burgdorferi","author":"Stricker","year":"2011","journal-title":"Infect Drug Resist"},{"key":"10.1016\/j.mehy.2018.04.012_b0050","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/infdis\/166.2.440","article-title":"Fibroblasts protect the Lyme disease spirochete, Borrelia burgdorferi, from ceftriaxone in vitro","volume":"166","author":"Georgilis","year":"1992","journal-title":"J Infect Dis"},{"key":"10.1016\/j.mehy.2018.04.012_b0055","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1086\/382893","article-title":"Borrelia-specific interferon-\u03b3 and interleukin-4 secretion in cerebrospinal fluid and blood during lyme borreliosis in humans: association with clinical outcome","volume":"189","author":"Widhe","year":"2004","journal-title":"J Infect Dis"},{"key":"10.1016\/j.mehy.2018.04.012_b0060","first-page":"e1004976","article-title":"Suppression of long-lived humoral immunity following Borrelia burgdorferi infection","volume":"11","author":"Elsner","year":"2015"},{"key":"10.1016\/j.mehy.2018.04.012_b0065","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/BF01716077","article-title":"Borrelia burgdorferi DNA in the urine of treated patients with chronic Lyme disease symptoms. A PCR study of 97 cases","volume":"24","author":"Bayer","year":"1996","journal-title":"Infection"},{"key":"10.1016\/j.mehy.2018.04.012_b0070","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1016\/j.cll.2015.07.005","article-title":"Alternatives to serologic testing for diagnosis of lyme disease","volume":"35","author":"Alby","year":"2015","journal-title":"Clin Lab Med"},{"key":"10.1016\/j.mehy.2018.04.012_b0075","doi-asserted-by":"crossref","first-page":"663","DOI":"10.17219\/acem\/28625","article-title":"Lyme borreliosis \u2013 the utility of improved real-time PCR assay in detection of Borrelia burgdorferi infections","volume":"24","author":"Bil-Lula","year":"2015","journal-title":"Adv Clin Exp Med"},{"key":"10.1016\/j.mehy.2018.04.012_b0080","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1089\/vbz.2016.2057","article-title":"Induction of apoptosis in normal human dermal fibroblasts infected with Borrelia burgdorferi sensu lato","volume":"17","author":"Rozwadowska","year":"2017","journal-title":"Vector-Borne Zoonotic Dis"},{"key":"10.1016\/j.mehy.2018.04.012_b0085","first-page":"1338","article-title":"Invasion of eukaryotic cells by Borrelia burgdorferi requires \u03b2 1 integrins and src kinase activity","volume":"79","author":"Wu","year":"2011"},{"key":"10.1016\/j.mehy.2018.04.012_b0090","doi-asserted-by":"crossref","first-page":"269","DOI":"10.33073\/pjm-2011-038","article-title":"Inhibition of fibroblast apoptosis by Borrelia afzelii, Coxiella burnetii and Bartonella henselae","volume":"60","author":"Chmielewski","year":"2011","journal-title":"Pol J Microbiol"},{"key":"10.1016\/j.mehy.2018.04.012_b0095","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1556\/1886.2015.00031","article-title":"Effectiveness of Stevia rebaudiana whole leaf extract against the various morphological forms of Borrelia burgdorferi in vitro","volume":"5","author":"Theophilus","year":"2015","journal-title":"Eur J Microbiol Immunol"},{"key":"10.1016\/j.mehy.2018.04.012_b0100","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1016\/j.nano.2017.06.011","article-title":"Pulmonary delivery of synergistic combination of fluoroquinolone antibiotic complemented with proteolytic enzyme: a novel antimicrobial and antibiofilm strategy","volume":"13","author":"Gupta","year":"2017","journal-title":"Nanomed Nanotechnol Biol Med"},{"key":"10.1016\/j.mehy.2018.04.012_b0105","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1128\/AAC.37.12.2618","article-title":"Proteolytic enzymes: a new treatment strategy for prosthetic infections?","volume":"37","author":"Selan","year":"1993","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.mehy.2018.04.012_b0110","doi-asserted-by":"crossref","first-page":"46","DOI":"10.4269\/ajtmh.16-0434","article-title":"The Jarisch-Herxheimer reaction after antibiotic treatment of spirochetal infections: a review of recent cases and our understanding of pathogenesis","volume":"96","author":"Butler","year":"2017","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.mehy.2018.04.012_b0115","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s10529-014-1734-8","article-title":"Microfluidic-based isolation of bacteria from whole blood for sepsis diagnostics","volume":"37","author":"Zelenin","year":"2015","journal-title":"Biotechnol Lett"},{"key":"10.1016\/j.mehy.2018.04.012_b0120","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1002\/cyto.990120612","article-title":"Membrane cell permeabilization with saponin and multiparametric analysis by flow cytometry","volume":"12","author":"Jacob","year":"1991","journal-title":"Cytometry"},{"key":"10.1016\/j.mehy.2018.04.012_b0125","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-1-59745-324-0_9","article-title":"Permeabilization of cell membranes","volume":"588","author":"Jamur","year":"2010","journal-title":"Methods Mol Biol"}],"container-title":["Medical Hypotheses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0306987718302421?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0306987718302421?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T19:44:04Z","timestamp":1604173444000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0306987718302421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":25,"alternative-id":["S0306987718302421"],"URL":"https:\/\/doi.org\/10.1016\/j.mehy.2018.04.012","relation":{},"ISSN":["0306-9877"],"issn-type":[{"type":"print","value":"0306-9877"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Blood cell disruption to significantly improve the Borrelia PCR detection sensitivity in borreliosis in humans","name":"articletitle","label":"Article Title"},{"value":"Medical Hypotheses","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mehy.2018.04.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}