{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T20:43:16Z","timestamp":1793911396270,"version":"4.8.0"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","metabolismjournal.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Metabolism"],"published-print":{"date-parts":[[2020,1]]},"DOI":"10.1016\/j.metabol.2019.154002","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T20:26:09Z","timestamp":1573244769000},"page":"154002","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":131,"special_numbering":"C","title":["Protective effects of sulforaphane on type 2 diabetes-induced cardiomyopathy via AMPK-mediated activation of lipid metabolic pathways and NRF2 function"],"prefix":"10.1016","volume":"102","author":[{"given":"Yike","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"9","key":"10.1016\/j.metabol.2019.154002_bb0005","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1007\/s11906-017-0770-x","article-title":"Hyperuricemia, type 2 diabetes mellitus, and hypertension: an emerging association","volume":"19","author":"Mortada","year":"2017","journal-title":"Curr Hypertens Rep"},{"key":"10.1016\/j.metabol.2019.154002_bb0010","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.vph.2015.09.002","article-title":"Globular adiponectin controls insulin-mediated vasoreactivity in muscle through ampkalpha2","volume":"78","author":"de Boer","year":"2016","journal-title":"Vascul Pharmacol"},{"issue":"11","key":"10.1016\/j.metabol.2019.154002_bb0015","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1111\/eci.12673","article-title":"Diabetic cardiomyopathy: a clinical entity or a cluster of molecular heart changes?","volume":"46","author":"Mizamtsidi","year":"2016","journal-title":"Eur J Clin Invest"},{"key":"10.1016\/j.metabol.2019.154002_bb0020","article-title":"Cardiomyopathy in obesity, insulin resistance and diabetes","author":"Nakamura","year":"2019","journal-title":"J Physiol"},{"key":"10.1016\/j.metabol.2019.154002_bb0025","series-title":"Prog Cardiovasc Dis","article-title":"Diabetic cardiomyopathy - a comprehensive updated review","author":"Murtaza","year":"2019"},{"issue":"2","key":"10.1016\/j.metabol.2019.154002_bb0030","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/s41419-018-0307-5","article-title":"Fibroblast growth factor-21 prevents diabetic cardiomyopathy via ampk-mediated antioxidation and lipid-lowering effects in the heart","volume":"9","author":"Yang","year":"2018","journal-title":"Cell Death Dis"},{"issue":"5","key":"10.1016\/j.metabol.2019.154002_bb0035","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.cmet.2009.03.012","article-title":"An emerging drug target for diabetes and the metabolic syndrome","volume":"9","author":"Zhang","year":"2009","journal-title":"Cell Metab"},{"key":"10.1016\/j.metabol.2019.154002_bb0040","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ceb.2017.01.005","article-title":"Ampk signalling in health and disease","volume":"45","author":"Carling","year":"2017","journal-title":"Curr Opin Cell Biol"},{"key":"10.1016\/j.metabol.2019.154002_bb0045","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1042\/bst0310216","article-title":"Physiological role of AMP-activated protein kinase (AMPK): insights from knockout mouse models","volume":"31","author":"Viollet","year":"2003","journal-title":"Biochem Soc Trans"},{"key":"10.1016\/j.metabol.2019.154002_bb0050","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1038\/sj.emboj.7600667","article-title":"Deficiency of LKB1 in skeletal muscle prevents AMPK activation and glucose uptake during contraction","volume":"24","author":"Sakamoto","year":"2005","journal-title":"EMBO J"},{"issue":"3","key":"10.1016\/j.metabol.2019.154002_bb0055","first-page":"227","article-title":"Lkb1 and ampk and the regulation of skeletal muscle metabolism","volume":"11","author":"Koh","year":"2008","journal-title":"Curr Opin Clin Nutr Metab Care"},{"key":"10.1016\/j.metabol.2019.154002_bb0060","article-title":"Arctigenin improves lipid metabolism by regulating amp-activated protein kinase and downstream signaling pathways","author":"Song","year":"2019","journal-title":"J Cell Biochem"},{"issue":"1","key":"10.1016\/j.metabol.2019.154002_bb0065","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.bbrc.2005.12.011","article-title":"Ampk activation increases fatty acid oxidation in skeletal muscle by activating pparalpha and pgc-1","volume":"340","author":"Lee","year":"2006","journal-title":"Biochem Biophys Res Commun"},{"issue":"4","key":"10.1016\/j.metabol.2019.154002_bb0070","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s00018-003-3216-3","article-title":"Peroxisome proliferator-activated receptor alpha target genes","volume":"61","author":"Mandard","year":"2004","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.metabol.2019.154002_bb0075","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1128\/MCB.20.5.1868-1876.2000","article-title":"The Coactivator PGC-1 cooperates with peroxisome proliferator-activated Recptor \u03b1 in transcriptional control of nuclear genes encoding mitochondrial fatty acid oxidation enzymes","volume":"20","author":"Vega","year":"2000","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.metabol.2019.154002_bb0080","doi-asserted-by":"crossref","first-page":"8560","DOI":"10.1074\/jbc.273.15.8560","article-title":"Control of human muscle-type Carnitine palmitoyltransferase I gene transcription by peroxisome proliferator-activated receptor","volume":"273","author":"Mascar\u00f3","year":"1998","journal-title":"J Biol Chem"},{"issue":"11","key":"10.1016\/j.metabol.2019.154002_bb0085","doi-asserted-by":"crossref","DOI":"10.3390\/ijms19113447","article-title":"Pgc-1alpha as a pivotal factor in lipid and metabolic regulation","volume":"19","author":"Cheng","year":"2018","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.metabol.2019.154002_bb0090","doi-asserted-by":"crossref","unstructured":"Huang TY, Zheng D, Houmard JA, Brault JJ, Hickner RC, Cortright RN. Overexpression of pgc-1alpha increases peroxisomal activity and mitochondrial fatty acid oxidation in human primary myotubes. Am J Physiol Endocrinol Metab 2017;312(4): E253-E63.","DOI":"10.1152\/ajpendo.00331.2016"},{"issue":"8","key":"10.1016\/j.metabol.2019.154002_bb0095","doi-asserted-by":"crossref","DOI":"10.3390\/nu10081024","article-title":"Paeoniflorin ameliorates fructose-induced insulin resistance and hepatic steatosis by activating lkb1\/ampk and akt pathways","volume":"10","author":"Li","year":"2018","journal-title":"Nutrients"},{"key":"10.1016\/j.metabol.2019.154002_bb0100","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.freeradbiomed.2016.11.009","article-title":"Nrf2-mediated liver protection by esculentoside a against acetaminophen toxicity through the ampk\/akt\/gsk3beta pathway","volume":"101","author":"Wang","year":"2016","journal-title":"Free Radic Biol Med"},{"issue":"1","key":"10.1016\/j.metabol.2019.154002_bb0105","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s12974-019-1406-7","article-title":"Neuroprotective effect of apelin 13 on ischemic stroke by activating ampk\/gsk-3beta\/nrf2 signaling","volume":"16","author":"Duan","year":"2019","journal-title":"J Neuroinflammation"},{"key":"10.1016\/j.metabol.2019.154002_bb0110","doi-asserted-by":"crossref","first-page":"382","DOI":"10.3389\/fphar.2019.00382","article-title":"Nrf2 as a potential mediator of cardiovascular risk in metabolic diseases","volume":"10","author":"da Costa","year":"2019","journal-title":"Front Pharmacol"},{"issue":"1","key":"10.1016\/j.metabol.2019.154002_bb0115","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1053\/j.seminoncol.2015.09.013","article-title":"Frugal chemoprevention: targeting nrf2 with foods rich in sulforaphane","volume":"43","author":"Yang","year":"2016","journal-title":"Semin Oncol"},{"key":"10.1016\/j.metabol.2019.154002_bb0120","doi-asserted-by":"crossref","unstructured":"Lei P, Tian S, Teng C, Huang L, Liu X, Wang J, et al. Sulforaphane improves lipid metabolism by enhancing mitochondrial function and biogenesis in vivo and in vitro. Mol Nutr Food Res 2019;63(4): e1800795.","DOI":"10.1002\/mnfr.201800795"},{"key":"10.1016\/j.metabol.2019.154002_bb0125","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.yjmcc.2014.09.022","article-title":"Sulforaphane prevents the development of cardiomyopathy in type 2 diabetic mice probably by reversing oxidative stress-induced inhibition of lkb1\/ampk pathway","volume":"77","author":"Zhang","year":"2014","journal-title":"J Mol Cell Cardiol"},{"issue":"9","key":"10.1016\/j.metabol.2019.154002_bb0130","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/S2213-8587(19)30076-2","article-title":"Understanding the mechanisms of reversal of type 2 diabetes","volume":"7","author":"Taylor","year":"2019","journal-title":"The Lancet Diabetes & Endocrinology"},{"key":"10.1016\/j.metabol.2019.154002_bb0135","article-title":"Broccoli sprout extract prevents diabetic cardiomyopathy via nrf2 activation in db\/db t2dm mice","volume":"6","author":"Xu","year":"2016","journal-title":"Sci Rep"},{"issue":"6","key":"10.1016\/j.metabol.2019.154002_bb0140","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/nprot.2013.055","article-title":"Imaging of neutral lipids by oil red o for analyzing the metabolic status in health and disease","volume":"8","author":"Mehlem","year":"2013","journal-title":"Nat Protoc"},{"issue":"2","key":"10.1016\/j.metabol.2019.154002_bb0145","doi-asserted-by":"crossref","first-page":"353","DOI":"10.2337\/db14-0582","article-title":"Regulation of ampk activation by cd36 links fatty acid uptake to beta-oxidation","volume":"64","author":"Samovski","year":"2015","journal-title":"Diabetes"},{"issue":"9","key":"10.1016\/j.metabol.2019.154002_bb0150","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.1161\/CIRCRESAHA.118.314607","article-title":"Mitophagy is essential for maintaining cardiac function during high fat diet-induced diabetic cardiomyopathy","volume":"124","author":"Tong","year":"2019","journal-title":"Circ Res"},{"issue":"2","key":"10.1016\/j.metabol.2019.154002_bb0155","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1038\/nrm.2017.95","article-title":"Ampk: Guardian of metabolism and mitochondrial homeostasis","volume":"19","author":"Herzig","year":"2018","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"10","key":"10.1016\/j.metabol.2019.154002_bb0160","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1016\/j.bbalip.2016.03.018","article-title":"The role of cd36 in the regulation of myocardial lipid metabolism","volume":"1861","author":"Kim","year":"2016","journal-title":"Biochim Biophys Acta"},{"issue":"2","key":"10.1016\/j.metabol.2019.154002_bb0165","doi-asserted-by":"crossref","first-page":"E491","DOI":"10.1152\/ajpendo.00419.2001","article-title":"David J. Dyck, Xiao-Xia Han, Narendra N. Tandon, yoga Arumugam, Jan F. C. Glatz, et al. insulin induces the translocation of the fatty acid transporter FAT\/CD36 to the plasma membrane","volume":"282","author":"Luiken","year":"2002","journal-title":"Am J Physiol Endocrinol Metab"},{"issue":"4","key":"10.1016\/j.metabol.2019.154002_bb0170","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1194\/jlr.M090969","article-title":"Cd36 plays a negative role in the regulation of lipophagy in hepatocytes through an ampk-dependent pathway","volume":"60","author":"Li","year":"2019","journal-title":"J Lipid Res"},{"key":"10.1016\/j.metabol.2019.154002_bb0175","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1750-2187-5-15","article-title":"Ampk-induced activation of akt by aicar is mediated by igf-1r dependent and independent mechanisms in acute lymphoblastic leukemia","volume":"5","author":"Leclerc","year":"2010","journal-title":"J Mol Signal"},{"key":"10.1016\/j.metabol.2019.154002_bb0180","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1152\/japplphysiol.00853.2003","article-title":"Exercise training attenuated the PKB and GSK-3 dephosphorylation in the myocardium of ZDF rats","volume":"96","author":"Lajoie","year":"2004","journal-title":"J Appl Physiol"},{"issue":"14","key":"10.1016\/j.metabol.2019.154002_bb0185","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1128\/MCB.00118-16","article-title":"Ampk facilitates nuclear accumulation of nrf2 by phosphorylating at serine 550","volume":"36","author":"Joo","year":"2016","journal-title":"Mol Cell Biol"},{"key":"10.1016\/j.metabol.2019.154002_bb0190","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.yjmcc.2013.01.008","article-title":"Prevention by sulforaphane of diabetic cardiomyopathy is associated with up-regulation of nrf2 expression and transcription activation","volume":"57","author":"Bai","year":"2013","journal-title":"J Mol Cell Cardiol"},{"issue":"2","key":"10.1016\/j.metabol.2019.154002_bb0195","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jnutbio.2013.10.007","article-title":"Sulforaphane attenuates obesity by inhibiting adipogenesis and activating the ampk pathway in obese mice","volume":"25","author":"Choi","year":"2014","journal-title":"J Nutr Biochem"},{"issue":"7","key":"10.1016\/j.metabol.2019.154002_bb0200","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.1016\/j.ymthe.2017.01.025","article-title":"Broccoli-derived nanoparticle inhibits mouse colitis by activating dendritic cell amp-activated protein kinase","volume":"25","author":"Deng","year":"2017","journal-title":"Mol Ther"},{"issue":"Pt 1","key":"10.1016\/j.metabol.2019.154002_bb0205","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1242\/jeb.107110","article-title":"Epigenetic linkage of aging","volume":"218","author":"Daniel","year":"2015","journal-title":"cancer and nutrition J Exp Biol"},{"key":"10.1016\/j.metabol.2019.154002_bb0210","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.lfs.2019.04.002","article-title":"Metformin promotes autophagy in ischemia\/reperfusion myocardium via cytoplasmic ampkalpha1 and nuclear ampkalpha2 pathways","volume":"225","author":"Wang","year":"2019","journal-title":"Life Sci"},{"issue":"3","key":"10.1016\/j.metabol.2019.154002_bb0215","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0018450","article-title":"AMPK regulates circadian rhythms in a tissue- and isoform-specific manner","volume":"6","author":"Um","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.metabol.2019.154002_bb0220","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1172\/JCI16567","article-title":"The AMP-activated protein kinase a2 catalytic subunit controls whole-body insulin sensitivity","volume":"111","author":"Viollet","year":"2003","journal-title":"J Clin Invest"},{"issue":"11","key":"10.1016\/j.metabol.2019.154002_bb0225","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.2337\/db09-1716","article-title":"Amp-activated protein kinase alpha2 subunit is required for the preservation of hepatic insulin sensitivity by n-3 polyunsaturated fatty acids","volume":"59","author":"Jelenik","year":"2010","journal-title":"Diabetes"},{"key":"10.1016\/j.metabol.2019.154002_bb0230","doi-asserted-by":"crossref","unstructured":"Wang YG, Han XG, Yang Y, Qiao H, Dai KR, Fan QM, et al. Functional differences between ampk alpha1 and alpha2 subunits in osteogenesis, osteoblast-associated induction of osteoclastogenesis, and adipogenesis. Sci Rep 2016;6: 32771.","DOI":"10.1038\/srep32771"},{"key":"10.1016\/j.metabol.2019.154002_bb0235","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.metabol.2018.10.003","article-title":"Role of the alpha2 subunit of amp-activated protein kinase and its nuclear localization in mitochondria and energy metabolism-related gene expressions in c2c12 cells","volume":"90","author":"Okamoto","year":"2019","journal-title":"Metabolism"},{"issue":"9","key":"10.1016\/j.metabol.2019.154002_bb0240","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1007\/s00125-012-2617-y","article-title":"Mouse hypothalamic gt1-7 cells demonstrate ampk-dependent intrinsic glucose-sensing behaviour","volume":"55","author":"Beall","year":"2012","journal-title":"Diabetologia"}],"container-title":["Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026049519302173?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0026049519302173?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T04:22:07Z","timestamp":1758428527000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0026049519302173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":48,"alternative-id":["S0026049519302173"],"URL":"https:\/\/doi.org\/10.1016\/j.metabol.2019.154002","relation":{},"ISSN":["0026-0495"],"issn-type":[{"value":"0026-0495","type":"print"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protective effects of sulforaphane on type 2 diabetes-induced cardiomyopathy via AMPK-mediated activation of lipid metabolic pathways and NRF2 function","name":"articletitle","label":"Article Title"},{"value":"Metabolism","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.metabol.2019.154002","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"154002"}}