{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:16:59Z","timestamp":1762409819670,"version":"build-2065373602"},"reference-count":17,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2019,10,10]],"date-time":"2019-10-10T00:00:00Z","timestamp":1570665600000},"content-version":"vor","delay-in-days":282,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Edital 01\/2016"],"award-info":[{"award-number":["Edital 01\/2016"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2019]]},"DOI":"10.1016\/j.mex.2019.10.007","type":"journal-article","created":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T12:48:58Z","timestamp":1571662138000},"page":"2544-2552","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Benznidazole, itraconazole and their combination in the treatment of acute experimental Chagas disease in dogs"],"prefix":"10.1016","volume":"6","author":[{"given":"Eleonora Lima Alves","family":"Cunha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda Karoline Vieira da Silva","family":"Torchelsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Maciel","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maykon Tavares","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Levi Eduardo Soares","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00e1tia da Silva","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Melo Abreu","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Martins","family":"Carneiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1565-4707","authenticated-orcid":false,"given":"Marta","family":"Lana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mex.2019.10.007_bib0005","first-page":"389","article-title":"Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi","volume":"4","author":"Brener","year":"1962","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"10.1016\/j.mex.2019.10.007_bib0010","article-title":"Estudios sobre inmunobiolog\u00eda de la enfermedades parasitarias: I influencia de la constituicion genetica en la resistencia de las lauchas a la infeccion experimental pelo Trypanosoma cruzi","author":"Pizzi","year":"1940","journal-title":"Bol Inf Parasit Chile"},{"key":"10.1016\/j.mex.2019.10.007_bib0015","first-page":"7","article-title":"II Consenso Brasileiro em Doen\u00e7a de Chagas, 2015","volume":"25","author":"Classic Cure Control: Second Brazilian Consensus on Chagas Disease","year":"2016","journal-title":"Epidemiol. Serv. Sa\u00fade"},{"key":"10.1016\/j.mex.2019.10.007_bib0020","first-page":"93","article-title":"Growth and diferentiation in Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media","volume":"12","author":"Camargo","year":"1964","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"10.1016\/j.mex.2019.10.007_bib0025","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/0166-6851(91)90116-N","article-title":"Polymerase chain reaction amplification of Trypanosoma cruzi kinetoplast minicircle DNA isolation from whole blood lysates: diagnosis of chronic Chagas\u2019 disease","volume":"40","author":"\u00c1vila","year":"1991","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.mex.2019.10.007_bib0030","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1590\/S0074-02761993000100030","article-title":"A simple protocol for cleavage of Trypanosoma cruzi kinetoplast DNA present in blood sample and its use in polymerase chain reaction (PCR)-based diagnosis of chronic Chagas\u2019 disease","volume":"88","author":"Britto","year":"1993","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.mex.2019.10.007_bib0035","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1006\/expr.1998.4191","article-title":"Trypanosoma cruzi: optimization of polymerase chain reaction for detection in human blood","volume":"88","author":"Gomes","year":"1998","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.mex.2019.10.007_bib0040","first-page":"183","article-title":"Evaluation of Trypanosoma cruzi antigens for the indirect hemagglutination reaction II. Antigens of different samplings and evolutive forms","volume":"29","author":"Victor","year":"1987","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"10.1016\/j.mex.2019.10.007_bib0045","first-page":"55","article-title":"Enzyme immunoassays in diagnostic medicine. Theory and practice","volume":"53","author":"Voller","year":"1976","journal-title":"Bull. World Health Organ."},{"key":"10.1016\/j.mex.2019.10.007_bib0050","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1590\/S0037-86822012000100008","article-title":"In-house ELISA method to analyze anti-Trypanosoma cruzi para o diagn\u00f3stico diferencial e avalia\u00e7\u00e3o da morbidade da doen\u00e7a de Chagas","volume":"45","author":"Santos","year":"2012","journal-title":"Ver. Soc. Bras. Med. Trop."},{"key":"10.1016\/j.mex.2019.10.007_bib0055","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mex.2019.10.007_bib0060","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1590\/S0074-02762009000900020","article-title":"The utility of anti-trypomastigote lytic antibodies for determining cure of Trypanosoma cruzi infections in treated patients: an overview and perspectives","volume":"104","author":"Krettli","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"10.1016\/j.mex.2019.10.007_bib0065","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/tmi.12002","article-title":"Myocardial scars correlate with eletrocardiographic changes in chronic Trypanosoma cruzi infection for dogs treated with Benznidazole","volume":"18","author":"Caldas","year":"2013","journal-title":"Trop. Med. Int. Health"},{"key":"10.1016\/j.mex.2019.10.007_bib0070","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.archoralbio.2004.01.004","article-title":"Vascular and cellular responses to pro-inflammatory stimuli in rat dental pulp","volume":"49","author":"Maltos","year":"2004","journal-title":"Arch. Oral Biol."},{"key":"10.1016\/j.mex.2019.10.007_bib0075","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1089\/pho.2008.2237","article-title":"Effect of visible and infrared polarized light on the healing process of full-thickness skin wounds: an experimental study","volume":"27","author":"Iordanou","year":"2009","journal-title":"Photomed. Laser Surg."},{"key":"10.1016\/j.mex.2019.10.007_bib0080","first-page":"170","article-title":"Real-time PCR strategy for parasite quantification in blood and tissue samples of experimental Trypanosoma cruzi infection","volume":"123","author":"Caldas","year":"2012","journal-title":"Acta Med. Ital. Med. Trop. Subtrop. Gastroenterol."},{"key":"10.1016\/j.mex.2019.10.007_bib0085","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0166-6851(03)00093-8","article-title":"Rapid quantitation of Trypanosoma cruzi in host tissue by real-time PCR","volume":"129","author":"Cummings","year":"2003","journal-title":"Mol. Biochem. Parasitol."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016119302729?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016119302729?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:02:43Z","timestamp":1762408963000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016119302729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019]]},"references-count":17,"alternative-id":["S2215016119302729"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2019.10.007","relation":{},"ISSN":["2215-0161"],"issn-type":[{"type":"print","value":"2215-0161"}],"subject":[],"published":{"date-parts":[[2019]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Benznidazole, itraconazole and their combination in the treatment of acute experimental Chagas disease in dogs","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2019.10.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"https:\/\/doi.org\/10.1016\/j.exppara.2019.05.005","name":"associatedlink","label":"CrossRef DOI link to the associated document"},{"value":"simple-article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}]}}