{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:46:26Z","timestamp":1792622786788,"version":"4.5.0"},"reference-count":9,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":137,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["19OC0054454"],"award-info":[{"award-number":["19OC0054454"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007797","name":"Helsingin Yliopisto","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007797","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014438","name":"Business Finland","doi-asserted-by":"publisher","award":["40915\/31\/2020"],"award-info":[{"award-number":["40915\/31\/2020"]}],"id":[{"id":"10.13039\/501100014438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2022]]},"DOI":"10.1016\/j.mex.2022.101737","type":"journal-article","created":{"date-parts":[[2022,5,23]],"date-time":"2022-05-23T13:19:40Z","timestamp":1653311980000},"page":"101737","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["An improved RT-qPCR method for direct quantification of enveloped RNA viruses"],"prefix":"10.1016","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0737-8845","authenticated-orcid":false,"given":"Pavlina","family":"Gregorova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minna-Maria K.","family":"Heinonen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4400-3601","authenticated-orcid":false,"given":"L. Peter","family":"Sarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"12","key":"10.1016\/j.mex.2022.101737_bib0001","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens10121558","article-title":"Direct viral RNA detection of SARS-CoV-2 and DENV in inactivated samples by real-time RT-qPCR: implications for diagnosis in resource limited settings with flavivirus Co-circulation","volume":"10","author":"Mao","year":"2021","journal-title":"Pathogens"},{"issue":"3","key":"10.1016\/j.mex.2022.101737_bib0002","doi-asserted-by":"crossref","DOI":"10.3390\/v14030508","article-title":"Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification","volume":"14","author":"Craig","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.mex.2022.101737_bib0003","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2020.587269","article-title":"SARS-CoV-2: structure, biology, and structure-based therapeutics development","volume":"10","author":"Wang","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"10","key":"10.1016\/j.mex.2022.101737_bib0004","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1080\/02786826.2010.501351","article-title":"Evaluation of filters for the sampling and quantification of RNA phage aerosols","volume":"44","author":"Gendron","year":"2010","journal-title":"Aerosol Sci. Technol."},{"issue":"14","key":"10.1016\/j.mex.2022.101737_bib0005","doi-asserted-by":"crossref","first-page":"4242","DOI":"10.1128\/AEM.00767-14","article-title":"Comparison of five bacteriophages as models for viral aerosol studies","volume":"80","author":"Turgeon","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.mex.2022.101737_bib0006","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1007\/s10453-010-9166-y","article-title":"The Cystovirus phi6 as a simulant for Venezuelan equine encephalitis virus","volume":"26","author":"Phillpotts","year":"2010","journal-title":"Aerobiologia"},{"key":"10.1016\/j.mex.2022.101737_bib0007","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.bbrc.2020.11.080","article-title":"Structural stability of SARS-CoV-2 virus like particles degrades with temperature","volume":"534","author":"Sharma","year":"2021","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"1","key":"10.1016\/j.mex.2022.101737_bib0008","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1128\/MMBR.63.1.149-160.1999","article-title":"Precise packaging of the three genomic segments of the double-stranded-RNA bacteriophage phi6","volume":"63","author":"Mindich","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"issue":"1","key":"10.1016\/j.mex.2022.101737_bib0009","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.virusres.2003.12.009","article-title":"Self-assembly of double-stranded RNA bacteriophages","volume":"101","author":"Poranen","year":"2004","journal-title":"Virus Res."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016122001182?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016122001182?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:16:34Z","timestamp":1778865394000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016122001182"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"references-count":9,"alternative-id":["S2215016122001182"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2022.101737","relation":{},"ISSN":["2215-0161"],"issn-type":[{"value":"2215-0161","type":"print"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An improved RT-qPCR method for direct quantification of enveloped RNA viruses","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2022.101737","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"101737"}}