{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:36:31Z","timestamp":1791394591653,"version":"4.3.3"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006111","name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006111","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015061","name":"NOVA University Lisbon Institute of Hygiene and Tropical Medicine","doi-asserted-by":"publisher","award":["LA-REAL-LA\/P\/0117\/2020"],"award-info":[{"award-number":["LA-REAL-LA\/P\/0117\/2020"]}],"id":[{"id":"10.13039\/501100015061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.mex.2023.102435","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T18:23:54Z","timestamp":1697048634000},"page":"102435","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae"],"prefix":"10.1016","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1501-9479","authenticated-orcid":false,"given":"Fernando M.H.","family":"Cardoso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Elias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00eas","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Maur\u00edcio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.mex.2023.102435_bib0001","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/MMBR.67.4.657-685.2003","article-title":"RNA interference: biology, mechanism, and applications","volume":"67","author":"Agrawal","year":"2003","journal-title":"Microbiol. Mol. Biol. Rev."},{"issue":"3","key":"10.1016\/j.mex.2023.102435_bib0002","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1097\/00004424-198703000-00017","volume":"22","author":"Stanton","year":"1987","journal-title":"Interferon review. Invest Radiol."},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0003","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/imr.13022","article-title":"The molecular mechanism of RIG-I activation and signaling","volume":"304","author":"Thoresen","year":"2021","journal-title":"Immunol. Rev."},{"issue":"11","key":"10.1016\/j.mex.2023.102435_bib0004","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.1096\/fasebj.3.11.2550303","article-title":"Yeast virology","volume":"3","author":"Wickner","year":"1989","journal-title":"FASEB J."},{"issue":"10","key":"10.1016\/j.mex.2023.102435_bib0005","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1111\/j.1365-2958.1991.tb02078.x","article-title":"Yeast dsRNA viruses: replication and killer phenotypes","volume":"5","author":"Tipper","year":"1991","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.mex.2023.102435_bib0006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-394315-6.00001-5","article-title":"Viruses and prions of Saccharomyces cerevisiae","volume":"86","author":"Wickner","year":"2013","journal-title":"Adv. Virus Res."},{"key":"10.1016\/j.mex.2023.102435_bib0007","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/978-981-10-8456-0_3","article-title":"Viral RNA-dependent rna polymerases: a structural overview","volume":"88","author":"Ferrero","year":"2018","journal-title":"Subcell. Biochem."},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0008","first-page":"15","article-title":"Endogenous double-stranded RNA","volume":"7","author":"Sadeq","year":"2021","journal-title":"Noncoding RNA"},{"issue":"2","key":"10.1016\/j.mex.2023.102435_bib0009","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s11262-019-01635-5","article-title":"Endornaviruses: persistent dsRNA viruses with symbiotic properties in diverse eukaryotes","volume":"55","author":"Fukuhara","year":"2019","journal-title":"Virus Genes"},{"key":"10.1016\/j.mex.2023.102435_bib0010","series-title":"Virus Taxonomy","first-page":"523","article-title":"Partitiviridae","author":"Lesker","year":"2011"},{"key":"10.1016\/j.mex.2023.102435_bib0011","series-title":"Family - Totiviridae, Virus Taxonomy","first-page":"639","author":"King","year":"2012"},{"key":"10.1016\/j.mex.2023.102435_bib0012","series-title":"Narnaviruses in Encyclopedia of Virology","first-page":"621","author":"Esteban","year":"2021"},{"issue":"6","key":"10.1016\/j.mex.2023.102435_bib0013","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1002\/yea.848","article-title":"Occurrence of 20S RNA and 23S RNA replicons in industrial yeast strains and their variation under nutritional stress conditions","volume":"19","author":"L\u00f3pez","year":"2002","journal-title":"Yeast"},{"issue":"10","key":"10.1016\/j.mex.2023.102435_bib0014","doi-asserted-by":"crossref","first-page":"943","DOI":"10.3390\/v11100943","article-title":"Identification of viral infection by purifying and sequencing dsRNA","volume":"11","author":"Decker","year":"2019","journal-title":"Viruses"},{"issue":"6","key":"10.1016\/j.mex.2023.102435_bib0015","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1073\/pnas.55.6.1504","article-title":"Purification and properties of the replicative intermediate of the RNA bacteriophage R17","volume":"55","author":"Franklin","year":"1966","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"6","key":"10.1016\/j.mex.2023.102435_bib0016","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1128\/jvi.9.6.903-908.1972","article-title":"Assay for secondary structure in ribonucleic acid","volume":"9","author":"Engelhardt","year":"1972","journal-title":"J. Virol."},{"issue":"4","key":"10.1016\/j.mex.2023.102435_bib0017","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1073\/pnas.70.4.1069","article-title":"A nucleic acid associated with a killer strain of yeast","volume":"70","author":"Vodkin","year":"1973","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.mex.2023.102435_bib0018","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1007\/s10658-016-1014-7","article-title":"Extraction and electrophoretic analysis of large dsRNAs from desiccated plant tissues infected with plant viruses and biotrophic fungi","volume":"147","author":"Khankhum","year":"2017","journal-title":"Eur. J. Plant Pathol."},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0019","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-0934(83)90062-9","article-title":"A simple procedure for the extraction of double-stranded RNA from virus-infected plants","volume":"6","author":"Bar-Joseph","year":"1983","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mex.2023.102435_bib0020","first-page":"255","article-title":"Analysis of double-stranded RNA for plant virus diagnosis","volume":"74","author":"Valverde","year":"1990","journal-title":"Plant Dis."},{"issue":"4","key":"10.1016\/j.mex.2023.102435_bib0021","doi-asserted-by":"crossref","first-page":"610","DOI":"10.2144\/97234bm10","article-title":"Microgranular cellulose improves dsRNA recovery from plant nucleic acid extracts","volume":"23","author":"Choi","year":"1997","journal-title":"BioTechniques"},{"issue":"4986","key":"10.1016\/j.mex.2023.102435_bib0022","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/206779a0","article-title":"Chromatography of nucleic acids on hydroxyapatite","volume":"206","author":"Bernardi","year":"1965","journal-title":"Nature"},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0023","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/0003-2697(73)90287-X","article-title":"A simple method for the separation of single-stranded and double-stranded RNA on hydroxyapatite","volume":"55","author":"Kalmakoff","year":"1973","journal-title":"Anal. Biochem."},{"issue":"15","key":"10.1016\/j.mex.2023.102435_bib0024","doi-asserted-by":"crossref","first-page":"5039","DOI":"10.1128\/AEM.00204-10","article-title":"Hydroxyapatite-mediated separation of double-stranded DNA, single-stranded DNA, and RNA genomes from natural viral assemblages","volume":"76","author":"Andrews-Pfannkoch","year":"2010","journal-title":"Appl. Environ. Microb."},{"issue":"2","key":"10.1016\/j.mex.2023.102435_bib0025","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1264\/jsme2.ME14174","article-title":"A New fractionation and recovery method of viral genomes based on nucleic acid composition and structure using tandem column chromatography","volume":"30","author":"Urayama","year":"2015","journal-title":"Microbes Environ."},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0026","first-page":"1","article-title":"Isolation of viral double-stranded RNAs using a LiCl fractionation procedure","volume":"8","author":"Diaz-Ruiz","year":"1978","journal-title":"Prep. Biochem."},{"issue":"3","key":"10.1016\/j.mex.2023.102435_bib0027","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/0022-2836(67)90321-X","article-title":"Sinsheimer RL. Fractionation of nucleic acid on benzoylated-naphthoylated DEAE cellulose","volume":"26","author":"Sedat","year":"1967","journal-title":"J. Mol. Biol."},{"issue":"2","key":"10.1016\/j.mex.2023.102435_bib0028","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1093\/nar\/16.2.776","article-title":"A Sephadex column procedure for DNA isolation is also useful for detecting dsRNA","volume":"16","author":"Livore","year":"1988","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0029","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.chroma.2006.11.081","article-title":"Purification of plant viral and satellite double-stranded RNAs on DEAE monoliths","volume":"1144","author":"Krajaci\u0107","year":"2007","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.mex.2023.102435_bib0030","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.chroma.2012.12.050","article-title":"High-throughput purification of double-stranded RNA molecules using convective interaction media monolithic anion exchange columns","volume":"1278","author":"Romanovskaya","year":"2013","journal-title":"J. Chromatogr. A"},{"key":"10.1016\/j.mex.2023.102435_bib0031","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1471-2091-5-14","article-title":"RNA integrity as a quality indicator during the first steps of RNP purifications: a comparison of yeast lysis methods","volume":"5","author":"L\u00f3pez de Heredia","year":"2004","journal-title":"BMC Biochem."},{"issue":"9","key":"10.1016\/j.mex.2023.102435_bib0032","doi-asserted-by":"crossref","first-page":"4224","DOI":"10.1073\/pnas.75.9.4224","article-title":"Electron microscopic heteroduplex analysis of \"killer\" double-stranded RNA species from yeast","volume":"75","author":"Fried","year":"1978","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"10.1016\/j.mex.2023.102435_bib0033","first-page":"423","article-title":"A rapid method for isolation of double stranded RNA","volume":"17","author":"Flegr","year":"1987","journal-title":"Prep. Biochem."},{"issue":"4","key":"10.1016\/j.mex.2023.102435_bib0034","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1080\/15257770.2018.1533138","article-title":"The effects of pH and salts on nucleic acid partitioning during phenol extraction","volume":"38","author":"Xu","year":"2019","journal-title":"Nucleosides Nucleotides Nucleic Acids"},{"issue":"1","key":"10.1016\/j.mex.2023.102435_bib0035","doi-asserted-by":"crossref","first-page":"54","DOI":"10.2144\/96211bm11","article-title":"RNA\/DNA mini-prep from a single sample of orchid tissue","volume":"21","author":"Knapp","year":"1996","journal-title":"BioTechniques"},{"issue":"2","key":"10.1016\/j.mex.2023.102435_bib0036","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1039\/D0AY01498B","article-title":"Complete enzymatic digestion of double-stranded RNA to nucleosides enables accurate quantification of dsRNA","volume":"13","author":"Strezsak","year":"2021","journal-title":"Anal. Methods"},{"issue":"24","key":"10.1016\/j.mex.2023.102435_bib0037","doi-asserted-by":"crossref","first-page":"13567","DOI":"10.1021\/acs.analchem.7b04000","article-title":"Accurate quantification of nucleic acids using hypochromicity measurements in conjunction with uv spectrophotometry","volume":"89","author":"Nwokeoji","year":"2017","journal-title":"Anal. Chem."},{"issue":"3","key":"10.1016\/j.mex.2023.102435_bib0038","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0166-0934(92)90084-Q","article-title":"A one-tube, one manipulation RT-PCR reaction for detection of ross river virus","volume":"40","author":"Sellner","year":"1992","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.mex.2023.102435_bib0039","first-page":"68","article-title":"Genefisher\u2013software support for the detection of postulated genes","volume":"4","author":"Giegerich","year":"1996","journal-title":"Proc. Int. Conf. Intell. Syst. Mol. Biol."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016123004314?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016123004314?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:08:20Z","timestamp":1762409300000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016123004314"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":39,"alternative-id":["S2215016123004314"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2023.102435","relation":{},"ISSN":["2215-0161"],"issn-type":[{"value":"2215-0161","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2023.102435","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102435"}}