{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:25:14Z","timestamp":1762410314472,"version":"build-2065373602"},"reference-count":33,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["3M210428"],"award-info":[{"award-number":["3M210428"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004070","name":"Khalifa University of Science, Technology and Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004070","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["EU-ERC101055155","EU-ERC743074"],"award-info":[{"award-number":["EU-ERC101055155","EU-ERC743074"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011878","name":"Vlaamse regering","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011878","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.mex.2023.102446","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T03:24:35Z","timestamp":1697513075000},"page":"102446","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Detailed protocol for a corneal thermal cauterization-based (lymph-)angiogenesis assay in mice"],"prefix":"10.1016","volume":"11","author":[{"given":"Anh-Co K.","family":"Truong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa M.","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nora","family":"Dekoning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Bouch\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koen","family":"Veys","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baharak","family":"Hosseinkhani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mieke","family":"Dewerchin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Eelen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7961-1821","authenticated-orcid":false,"given":"Peter","family":"Carmeliet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.mex.2023.102446_bib0001","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1038\/35025220","article-title":"Angiogenesis in cancer and other diseases","volume":"407","author":"Carmeliet","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.mex.2023.102446_bib0002","first-page":"2224","article-title":"Angiogenesis and signal transduction in endothelial cells","volume":"61","author":"Mu\u00f1oz-Ch\u00e1puli","year":"2004","journal-title":"Cellular Molecul. Life Sci."},{"key":"10.1016\/j.mex.2023.102446_bib0003","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1038\/nature04480","article-title":"Lymphangiogenesis in development and human disease","volume":"438","author":"Alitalo","year":"2005","journal-title":"Nature"},{"key":"10.1016\/j.mex.2023.102446_bib0004","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0146-0005(00)80062-2","article-title":"Importance of angiogenesis in reproductive physiology","volume":"24","author":"Jaffe","year":"2000","journal-title":"Semin. Perinatol."},{"key":"10.1016\/j.mex.2023.102446_bib0005","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1002\/j.0022-0337.2002.66.8.tb03565.x","article-title":"Angiogenesis in health and disease: insights into basic mechanisms and therapeutic opportunities","volume":"66","author":"Polverini","year":"2002","journal-title":"J. Dent. Educ."},{"key":"10.1016\/j.mex.2023.102446_bib0006","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1590\/s0102-865020170080000002","article-title":"Corneal angiogenesis based on different protocols of alkaline cauterization in murine models","volume":"32","author":"Claros-Chacaltana","year":"2017","journal-title":"Acta Cir. Bras."},{"key":"10.1016\/j.mex.2023.102446_bib0007","doi-asserted-by":"crossref","DOI":"10.1016\/j.preteyeres.2022.101157","article-title":"The novel role of lymphatic vessels in the pathogenesis of ocular diseases","volume":"96","author":"Clahsen","year":"2023","journal-title":"Progr. Retinal Eye Res."},{"key":"10.1016\/j.mex.2023.102446_bib0008","first-page":"1625","article-title":"A model of angiogenesis in the mouse cornea","volume":"37","author":"Kenyon","year":"1996","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.mex.2023.102446_bib0009","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1365-2613.2008.00633.x","article-title":"A critical analysis of current in vitro and in vivo angiogenesis assays","volume":"90","author":"Staton","year":"2009","journal-title":"Int. J. Exp. Pathol."},{"key":"10.1016\/j.mex.2023.102446_bib0010","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/nprot.2011.359","article-title":"Mouse corneal lymphangiogenesis model","volume":"6","author":"Cao","year":"2011","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.mex.2023.102446_bib0011","doi-asserted-by":"crossref","DOI":"10.14440\/jbm.2015.78","article-title":"Ocular surgical models for immune and angiogenic responses","volume":"2","author":"Inomata","year":"2015","journal-title":"J. Biol. Methods"},{"key":"10.1016\/j.mex.2023.102446_bib0012","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/s42255-019-0087-y","article-title":"Role and therapeutic potential of dietary ketone bodies in lymph vessel growth","volume":"1","author":"Garc\u00eda-Caballero","year":"2019","journal-title":"Nat. Metab."},{"key":"10.1016\/j.mex.2023.102446_bib0013","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.cmet.2020.03.009","article-title":"Single-cell RNA sequencing maps endothelial metabolic plasticity in pathological angiogenesis","volume":"31","author":"Rohlenova","year":"2020","journal-title":"Cell Metab."},{"key":"10.1016\/j.mex.2023.102446_bib0014","doi-asserted-by":"crossref","first-page":"520","DOI":"10.30802\/AALAS-CM-19-000041","article-title":"A review of the effects of pain and analgesia on immune system function and inflammation: relevance for preclinical studies","volume":"69","author":"DeMarco","year":"2019","journal-title":"Comp. Med."},{"key":"10.1016\/j.mex.2023.102446_bib0015","doi-asserted-by":"crossref","DOI":"10.1016\/j.exer.2022.109036","article-title":"Therapeutic potential of topical administration of siRNAs against HIF-1\u03b1 for corneal neovascularization","volume":"219","author":"Peral","year":"2022","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.mex.2023.102446_bib0016","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.omtm.2021.06.007","article-title":"Efficacious, safe, and stable inhibition of corneal neovascularization by AAV-vectored anti-VEGF therapeutics","volume":"22","author":"Su","year":"2021","journal-title":"Mol. Ther. Methods Clin. Dev."},{"key":"10.1016\/j.mex.2023.102446_bib0017","first-page":"2719","article-title":"Transient downregulation of microRNA-206 protects alkali burn injury in mouse cornea by regulating connexin 43","volume":"8","author":"Li","year":"2015","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"10.1016\/j.mex.2023.102446_bib0018","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1038\/nature05249","article-title":"Corneal avascularity is due to soluble VEGF receptor-1","volume":"443","author":"Ambati","year":"2006","journal-title":"Nature"},{"key":"10.1016\/j.mex.2023.102446_bib0019","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.exer.2008.08.007","article-title":"Improved semiautomatic method for morphometry of angiogenesis and lymphangiogenesis in corneal flatmounts","volume":"87","author":"Bock","year":"2008","journal-title":"Exp. Eye Res."},{"key":"10.1016\/j.mex.2023.102446_bib0020","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1046\/j.1087-0024.2000.00014.x","article-title":"Angiogenesis in wound healing","volume":"5","author":"Tonnesen","year":"2000","journal-title":"J. Invest. Dermatol. Symp. Proc."},{"key":"10.1016\/j.mex.2023.102446_bib0021","first-page":"264","article-title":"Corneal angiogenic privilege: angiogenic and antiangiogenic factors in corneal avascularity, vasculogenesis, and wound healing","volume":"104","author":"Azar","year":"2006","journal-title":"Trans. Am. Ophthalmol. Soc."},{"key":"10.1016\/j.mex.2023.102446_bib0022","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1001\/archopht.1983.01040010472030","article-title":"Histopathology of corneal neovascularization","volume":"101","author":"Schanzlin","year":"1983","journal-title":"Arch. Ophthalmol."},{"key":"10.1016\/j.mex.2023.102446_bib0023","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1172\/JCI20465","article-title":"VEGF-A stimulates lymphangiogenesis and hemangiogenesis in inflammatory neovascularization via macrophage recruitment","volume":"113","author":"Cursiefen","year":"2004","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.mex.2023.102446_bib0024","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1167\/iovs.61.12.20","article-title":"Wound healing after alkali burn injury of the cornea involves Nox4-type NADPH oxidase","volume":"61","author":"Hakami","year":"2020","journal-title":"Invest Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.mex.2023.102446_bib0025","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1167\/tvst.9.6.14","article-title":"Observation of corneal wound healing and angiogenesis using low-vacuum scanning electron microscopy","volume":"9","author":"Arima","year":"2020","journal-title":"Transl. Vis. Sci. Technol."},{"key":"10.1016\/j.mex.2023.102446_bib0026","first-page":"157","article-title":"An evaluation of the role of leukocytes in the pathogenesis of experimentally induced corneal vascularization. III. Studies related to the vasoproliferative capability of polymorphonuclear leukocytes and lymphocytes","volume":"82","author":"Fromer","year":"1976","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.mex.2023.102446_bib0027","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/micc.12587","article-title":"Surface markers: an identity card of endothelial cells","volume":"27","author":"Ribatti","year":"2020","journal-title":"Microcirculation"},{"key":"10.1016\/j.mex.2023.102446_bib0028","first-page":"1196","article-title":"Detection of apoptotic cells using immunohistochemistry","volume":"2014","author":"Newbold","year":"2014"},{"key":"10.1016\/j.mex.2023.102446_bib0029","first-page":"311","article-title":"The Ki-67 protein\u00a0: from the known and the unknown","volume":"322","author":"Scholzen","year":"2000","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.mex.2023.102446_bib0030","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-018-29752-5","article-title":"Comparison of optical coherence tomography angiography to indocyanine green angiography and slit lamp photography for corneal vascularization in an animal model","volume":"8","author":"Stanzel","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mex.2023.102446_bib0031","first-page":"1","article-title":"Characterization of multi-cellular dynamics of angiogenesis and vascular remodelling by intravital imaging of the wounded mouse cornea","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.mex.2023.102446_bib0032","doi-asserted-by":"crossref","first-page":"5880","DOI":"10.1167\/iovs.17-22286","article-title":"Label-free in vivo imaging of corneal lymphatic vessels using microscopic optical coherence tomography","volume":"58","author":"Horstmann","year":"2017","journal-title":"Invest Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.mex.2023.102446_bib0033","first-page":"625","article-title":"Host immune cellular reactions in corneal neovascularization","volume":"9","author":"Abdelfattah","year":"2016","journal-title":"Int. J. Ophthalmol."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016123004429?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016123004429?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:11:38Z","timestamp":1762409498000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016123004429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":33,"alternative-id":["S2215016123004429"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2023.102446","relation":{},"ISSN":["2215-0161"],"issn-type":[{"type":"print","value":"2215-0161"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Detailed protocol for a corneal thermal cauterization-based (lymph-)angiogenesis assay in mice","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2023.102446","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102446"}}