{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:47:18Z","timestamp":1791928038458,"version":"4.3.4"},"reference-count":17,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004625","name":"Universiti Teknologi MARA","doi-asserted-by":"publisher","award":["600-UiTMSEL"],"award-info":[{"award-number":["600-UiTMSEL"]}],"id":[{"id":"10.13039\/501100004625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004625","name":"Universiti Teknologi MARA","doi-asserted-by":"publisher","award":["5\/4\/8"],"award-info":[{"award-number":["5\/4\/8"]}],"id":[{"id":"10.13039\/501100004625","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004625","name":"Universiti Teknologi MARA","doi-asserted-by":"publisher","award":["004\/2025"],"award-info":[{"award-number":["004\/2025"]}],"id":[{"id":"10.13039\/501100004625","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.mex.2026.104020","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T15:53:09Z","timestamp":1782575589000},"page":"104020","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Step-by-step En face O red oil method for aortic plaque staining and quantification in ApoE knockout mouse"],"prefix":"10.1016","volume":"17","author":[{"given":"Nurin Yasmin Mohd","family":"Khairudin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Vimala R.M.T","family":"Balasubramaniam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhaila Abd","family":"Muid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurdiyana","family":"Nasrudin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0044-2477","authenticated-orcid":false,"given":"Nasibah","family":"Azme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"4","key":"10.1016\/j.mex.2026.104020_bib0001","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1159\/000189795","article-title":"Rapid quantification of aortic lesions in ApoE\u2212\/\u2212 mice","volume":"46","author":"Beattie","year":"2009","journal-title":"J. Vasc. Res."},{"issue":"4","key":"10.1016\/j.mex.2026.104020_bib0002","doi-asserted-by":"crossref","first-page":"5298","DOI":"10.3892\/mmr.2015.4084","article-title":"Practical assessment of the quantification of atherosclerotic lesions in ApoE\u2212\/\u2212 mice","volume":"12","author":"Lin","year":"2015","journal-title":"Mol. Med. Rep."},{"issue":"11","key":"10.1016\/j.mex.2026.104020_bib0003","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1177\/002215549704501112","article-title":"Application of Photoshop-based image analysis to quantification of hormone receptor expression in breast cancer","volume":"45","author":"Lehr","year":"1997","journal-title":"J. Histochem. Cytochem."},{"issue":"1","key":"10.1016\/j.mex.2026.104020_bib0004","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1177\/002215549904700113","article-title":"Complete chromogen separation and analysis in double immunohistochemical stains using Photoshop-based image analysis","volume":"47","author":"Lehr","year":"1999","journal-title":"J. Histochem. Cytochem."},{"issue":"1","key":"10.1016\/j.mex.2026.104020_bib0005","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1160\/TH07-10-0595","article-title":"No effect of C-reactive protein on early atherosclerosis in LDLR\u2212\/\u2212\/human C-reactive protein transgenic mice","volume":"99","author":"Torzewski","year":"2008","journal-title":"Thromb. Haemost."},{"issue":"5","key":"10.1016\/j.mex.2026.104020_bib0006","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1194\/jlr.R067249","article-title":"ApoE knockout and knockin mice: the history of their contribution to the understanding of atherogenesis","volume":"57","author":"Getz","year":"2016","journal-title":"J. Lipid Res."},{"issue":"12","key":"10.1016\/j.mex.2026.104020_bib0007","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.3390\/genes11121427","article-title":"Atherosclerosis in different vascular locations unbiasedly approached with mouse genetics","volume":"11","author":"Kayashima","year":"2020","journal-title":"Genes"},{"issue":"1","key":"10.1016\/j.mex.2026.104020_bib0008","first-page":"72","article-title":"A mini-review on quantification of atherosclerosis in hypercholesterolemic mice","volume":"1","author":"Chen","year":"2022","journal-title":"Glob. Transl. Med."},{"key":"10.1016\/j.mex.2026.104020_bib0009","article-title":"Imaging and analysis of Oil Red O-stained whole aorta lesions in an aneurysm hyperlipidemia mouse model","volume":"183","author":"Chen","year":"2022","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.mex.2026.104020_bib0010","article-title":"Assessing atherosclerosis in the aorta from AAV8-PCSK9-injected mice by oil Red O staining","volume":"222","author":"Liu","year":"2025","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.mex.2026.104020_bib0011","doi-asserted-by":"crossref","DOI":"10.21769\/BioProtoc.1833","article-title":"Aorta atherosclerosis lesion analysis in hyperlipidemic mice","volume":"6","author":"Mohanta","year":"2016","journal-title":"Bio Protoc."},{"key":"10.1016\/j.mex.2026.104020_bib0012","series-title":"Methods in Mouse Atherosclerosis, Methods Model Biology 1339","first-page":"85","article-title":"Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root","author":"Andr\u00e9s-Manzano","year":"2015"},{"key":"10.1016\/j.mex.2026.104020_bib0013","series-title":"Atherosclerosis: Methods and Protocols","first-page":"481","article-title":"Evaluation of plaque burden and lipid content in atherosclerotic plaques","author":"Chan","year":"2022"},{"key":"10.1016\/j.mex.2026.104020_bib0014","series-title":"Transgenic Mouse: Methods and Protocols, Methods Model Biology","first-page":"293","article-title":"Quantification of atherosclerosis in mice","author":"Daugherty","year":"2003"},{"key":"10.1016\/j.mex.2026.104020_bib0015","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1476-511X-9-61","article-title":"Cholesterol in human atherosclerotic plaque is a marker for underlying disease state and plaque vulnerability","volume":"9","author":"Chen","year":"2010","journal-title":"Lipids Health Dis."},{"issue":"1","key":"10.1016\/j.mex.2026.104020_bib0016","doi-asserted-by":"crossref","first-page":"8251","DOI":"10.1038\/s41598-020-64821-8","article-title":"Comparison of three histological techniques for fat emboli detection in lung cetacean tissue","volume":"10","author":"Arregui","year":"2020","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.mex.2026.104020_bib0017","doi-asserted-by":"crossref","DOI":"10.1080\/21623945.2023.2179334","article-title":"An optimized method for oil red O staining with the salicylic acid ethanol solution","volume":"12","author":"Du","year":"2023","journal-title":"Adipocyte"}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016126002384?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016126002384?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:17:38Z","timestamp":1791926258000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016126002384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":17,"alternative-id":["S2215016126002384"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2026.104020","relation":{},"ISSN":["2215-0161"],"issn-type":[{"value":"2215-0161","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Step-by-step En face O red oil method for aortic plaque staining and quantification in ApoE knockout mouse","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2026.104020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104020"}}