{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:47:29Z","timestamp":1791928049474,"version":"4.3.4"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001721","name":"University of Groningen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001721","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["MethodsX"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.mex.2026.104082","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T15:13:06Z","timestamp":1785510786000},"page":"104082","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Serial Knockout Isolation Technique (SKIT): targeted isolation of multi-antagonist bacteria"],"prefix":"10.1016","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0221-8364","authenticated-orcid":false,"given":"Hemant Singh","family":"Maheshwari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aakash","family":"Gour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laxman Singh","family":"Rajput","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aalok","family":"Shiv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepika","family":"Parmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palak","family":"Solanki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeberlin Prabina","family":"Bright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahaveer Prasad","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"May, 5","key":"10.1016\/j.mex.2026.104082_bib0001","doi-asserted-by":"crossref","first-page":"01","DOI":"10.23910\/1.2024.5299","article-title":"Survey and present status of soybean diseases in central India","volume":"15","author":"Amrate","year":"2024","journal-title":"Int. J. Bio-Resour. Stress Manag."},{"issue":"10","key":"10.1016\/j.mex.2026.104082_bib0002","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1038\/s41579-023-00900-7","article-title":"Climate change impacts on plant pathogens, food security and paths forward","volume":"21","author":"Singh","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.mex.2026.104082_bib0003","article-title":"Isolation and characterization of new antagonistic bacteria P10-7 and evaluation of its biocontrol potential against tomato gray mold","volume":"16","author":"Chen","year":"2025","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.mex.2026.104082_bib0004","doi-asserted-by":"crossref","DOI":"10.1016\/j.pmpp.2025.102605","article-title":"Bacillus amyloliquefaciens D5 as a biocontrol agent against Cercospora Leaf Spot Disease in Vigna radiata L","volume":"137","author":"Kamath","year":"2025","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"10.1016\/j.mex.2026.104082_bib0005","doi-asserted-by":"crossref","DOI":"10.1007\/s40009-026-02071-4","article-title":"Prospecting streptomyces spp. isolated from tomato rhizosphere for biocontrol and plant growth promotion","author":"Pandiyan","year":"2026","journal-title":"Natl. Acad. Sci. Lett."},{"issue":"1","key":"10.1016\/j.mex.2026.104082_bib0006","doi-asserted-by":"crossref","DOI":"10.13057\/biotek\/c200104","article-title":"Enterobacter tabaci and Bacillus cereus as biocontrol agents against pathogenic Ralstonia solanacearum of eggplant","volume":"20","author":"MALEK","year":"2023","journal-title":"Asian J. Trop. Biotechnol."},{"issue":"1","key":"10.1016\/j.mex.2026.104082_bib0007","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s41938-020-00262-9","article-title":"Approving the biocontrol method of potato wilt caused by Ralstonia solanacearum (Smith) using Enterobacter cloacae PS14 and Trichoderma asperellum T34","volume":"30","author":"Mohamed","year":"2020","journal-title":"Egypt. J. Biol. Pest Control"},{"issue":"8","key":"10.1016\/j.mex.2026.104082_bib0008","first-page":"14","article-title":"Seed-borne probiotic yeasts foster plant growth and elicit health protection in black gram (Vigna mungo L.)","author":"Bright","year":"2022","journal-title":"Sustainability"},{"key":"10.1016\/j.mex.2026.104082_bib0009","doi-asserted-by":"crossref","DOI":"10.1016\/j.pestbp.2023.105681","article-title":"Characterization and biocontrol mechanism of Streptomyces olivoreticuli as a potential biocontrol agent against Rhizoctonia solani","volume":"197","author":"Zhong","year":"2023","journal-title":"Pestic. Biochem. Physiol."},{"key":"10.1016\/j.mex.2026.104082_bib0010","doi-asserted-by":"crossref","DOI":"10.3389\/fagro.2025.1578915","article-title":"Biological control agents: mechanisms of action, selection, formulation and challenges in agriculture","volume":"7","author":"Villavicencio-V\u00e1squez","year":"2025","journal-title":"Front. Agron."},{"key":"10.1016\/j.mex.2026.104082_bib0011","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.sajb.2023.12.031","article-title":"Siderophore producing bacteria as biocontrol agent against phytopathogens for a better environment: a review","volume":"165","author":"Deb","year":"2024","journal-title":"S. Afr. J. Bot."},{"key":"10.1016\/j.mex.2026.104082_bib0012","doi-asserted-by":"crossref","DOI":"10.1016\/j.mex.2025.103677","article-title":"Serial Enrichment Incubation Technique (SEIT) for rapid isolation of plant growth-promoting bacteria: saving researcher time and lab resources","volume":"15","author":"Maheshwari","year":"2025","journal-title":"MethodsX"},{"key":"10.1016\/j.mex.2026.104082_bib0013","unstructured":"Lehman Donald. (2005). Triple sugar iron agar protocols. https:\/\/asm.org\/asm\/media\/protocol-images\/triple-sugar-iron-agar-protocols.pdf?ext=.pdf."},{"issue":"1","key":"10.1016\/j.mex.2026.104082_bib0014","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0281102","article-title":"Thermo-neutrophilic cellulases and chitinases characterized from a novel putative antifungal biocontrol agent: Bacillus subtilis TD11","volume":"18","author":"Malik","year":"2023","journal-title":"PLoS One"},{"issue":"S1","key":"10.1016\/j.mex.2026.104082_bib0015","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/s10123-024-00490-1","article-title":"Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi","volume":"28","author":"Maral-G\u00fcl","year":"2024","journal-title":"Int. Microbiol."},{"key":"10.1016\/j.mex.2026.104082_bib0016","series-title":"Secondary Metabolites and Volatiles of PGPR in Plant-Growth Promotion","first-page":"303","article-title":"Hydrolytic enzyme producing plant growth-promoting rhizobacteria (PGPR) in plant growth promotion and biocontrol","author":"Reddy","year":"2022"},{"issue":"1\u20133","key":"10.1016\/j.mex.2026.104082_bib0017","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1385\/ABAB:118:1-3:243","article-title":"Siderophore production by a marine <I>Pseudomonas aeruginosa<\/I> and its antagonistic action against phytopathogenic fungi","volume":"118","author":"Manwar","year":"2004","journal-title":"Appl. Biochem. Biotechnol"},{"issue":"45","key":"10.1016\/j.mex.2026.104082_bib0018","doi-asserted-by":"crossref","first-page":"26723","DOI":"10.1074\/jbc.270.45.26723","article-title":"Siderophores: structure and function of microbial iron transport compounds","volume":"270","author":"Neilands","year":"1995","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.mex.2026.104082_bib0019","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.jenvrad.2015.12.015","article-title":"Root endophytic bacteria of a 137Cs and Mn accumulator plant, Eleutherococcus sciadophylloides, increase 137Cs and Mn desorption in the soil","volume":"153","author":"Yamaji","year":"2016","journal-title":"J. Environ. Radioact."},{"issue":"2","key":"10.1016\/j.mex.2026.104082_bib0020","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.1399-3054.1948.tb07118.x","article-title":"Production of hydrocyanic acid by bacteria","volume":"1","author":"Lorck","year":"1948","journal-title":"Physiol. Plant."},{"issue":"2","key":"10.1016\/j.mex.2026.104082_bib0021","doi-asserted-by":"crossref","first-page":"145","DOI":"10.5251\/abjna.2013.4.2.145.154","article-title":"Contribution in isolation and identification of some pathogenic fungi from wheat seeds, and evaluation of antagonistic capability of Trichoderma harzianum against those isolated fungi in vitro","volume":"4","author":"Mokhtar","year":"2013","journal-title":"Agric. Biol. J. N. Am."},{"issue":"1","key":"10.1016\/j.mex.2026.104082_bib0022","doi-asserted-by":"crossref","DOI":"10.1094\/PHP-2010-0102-01-RS","article-title":"Effect of diseases on soybean yield in the top eight producing countries in 2006","volume":"11","author":"Wrather","year":"2010","journal-title":"Plant Health Prog."},{"issue":"3","key":"10.1016\/j.mex.2026.104082_bib0023","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1007\/s42360-024-00765-7","article-title":"Distribution maps depicting geographical prevalence of rapeseed-mustard diseases in India","volume":"77","author":"Meena","year":"2024","journal-title":"Indian Phytopathol."},{"issue":"4","key":"10.1016\/j.mex.2026.104082_bib0024","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1094\/PDIS-06-18-0980-FE","article-title":"Comprehensive disease survey of U.S. sunflower: disease trends, research priorities and unanticipated impacts","volume":"103","author":"Gulya","year":"2019","journal-title":"Plant Dis."},{"key":"10.1016\/j.mex.2026.104082_bib0025","series-title":"Genomic Designing for Biotic Stress Resistant Pulse Crops","first-page":"117","article-title":"Chickpea biotic stresses","author":"Manjunatha","year":"2022"},{"key":"10.1016\/j.mex.2026.104082_bib0026","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.cropro.2015.08.021","article-title":"Management of soil borne diseases of groundnut through seed dressing fungicides","volume":"78","author":"Jadon","year":"2015","journal-title":"Crop Prot."}],"container-title":["MethodsX"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016126002980?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2215016126002980?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:20:47Z","timestamp":1791926447000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2215016126002980"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":26,"alternative-id":["S2215016126002980"],"URL":"https:\/\/doi.org\/10.1016\/j.mex.2026.104082","relation":{},"ISSN":["2215-0161"],"issn-type":[{"value":"2215-0161","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Serial Knockout Isolation Technique (SKIT): targeted isolation of multi-antagonist bacteria","name":"articletitle","label":"Article Title"},{"value":"MethodsX","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.mex.2026.104082","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"104082"}}