{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:51:49Z","timestamp":1790304709090,"version":"4.1.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T00:00:00Z","timestamp":1486425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Innovation Fund Denmark","award":["069-2011-1"],"award-info":[{"award-number":["069-2011-1"]}]},{"DOI":"10.13039\/501100006442","name":"Faculty of Health and Medical Sciences, University of Copenhagen","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006442","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbes and Infection"],"published-print":{"date-parts":[[2017,6]]},"DOI":"10.1016\/j.micinf.2017.01.008","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T16:16:45Z","timestamp":1486657005000},"page":"334-342","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus"],"prefix":"10.1016","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3505-1356","authenticated-orcid":false,"given":"Emma","family":"Lorenzen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Follmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan O.","family":"Secher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Goericke-Pesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette S.","family":"Hansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Zakariassen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja W.","family":"Olsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregers","family":"Jungersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f8rgen S.","family":"Agerholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micinf.2017.01.008_bib1","series-title":"Global incidence and prevalence of selected curable sexually transmitted infections","author":"World Health Organization","year":"2008"},{"key":"10.1016\/j.micinf.2017.01.008_bib2","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.2217\/fmb.10.148","article-title":"Towards a Chlamydia trachomatis vaccine: how close are we?","volume":"5","author":"Cochrane","year":"2010","journal-title":"Future Microbiol"},{"key":"10.1016\/j.micinf.2017.01.008_bib3","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1016\/j.vaccine.2013.08.032","article-title":"Current barriers, challenges and opportunities for the development of effective STI vaccines: point of view of vaccine producers, biotech companies and funding agencies","volume":"32","author":"Dodet","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2017.01.008_bib4","doi-asserted-by":"crossref","first-page":"3060","DOI":"10.1128\/IAI.00357-13","article-title":"Animal models for studying female genital tract infection with Chlamydia trachomatis","volume":"81","author":"De Clercq","year":"2013","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2017.01.008_bib5","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1177\/0192623314526136","article-title":"Estrous cycle-dependent morphology in the reproductive organs of the female Gottingen Minipig","volume":"42","author":"De Rijk","year":"2014","journal-title":"Toxicol Pathol"},{"key":"10.1016\/j.micinf.2017.01.008_bib6","first-page":"46","article-title":"A\u00a0review of the human vs. porcine female genital tract and associated immune system in the perspective of using minipigs as a model of human genital Chlamydia infection","author":"Lorenzen","year":"2015","journal-title":"Vet Res"},{"key":"10.1016\/j.micinf.2017.01.008_bib7","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/icb.2015.79","article-title":"A\u00a0multi-subunit Chlamydia vaccine inducing neutralizing antibodies and strong IFN-\u03b3+CMI responses protects against a genital infection in minipigs","volume":"94","author":"B\u00f8je","year":"2015","journal-title":"Immunol Cell Biol"},{"key":"10.1016\/j.micinf.2017.01.008_bib8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fimmu.2015.00628","article-title":"Intramuscular priming and intranasal boosting induce strong genital immunity through secretory IgA in minipigs infected with Chlamydia trachomatis","volume":"6","author":"Lorenzen","year":"2015","journal-title":"Front Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib9","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1186\/s12917-016-0793-6","article-title":"Genital tract lesions in sexually mature G\u00f6ttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D","volume":"12","author":"Erneholm","year":"2016","journal-title":"BMC Vet Res"},{"key":"10.1016\/j.micinf.2017.01.008_bib10","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/mi.2007.19","article-title":"Chlamydia trachomatis infection: host immune responses and potential vaccines","volume":"1","author":"Hafner","year":"2008","journal-title":"Mucosal Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib11","series-title":"Dellmann's textbook of veterinary histology","author":"Eurell","year":"2006"},{"key":"10.1016\/j.micinf.2017.01.008_bib12","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1574-6968.1981.tb07622.x","article-title":"Progesterone as a key factor in the development of a mouse model for genital-tract infection with Chlamydia trachomatis","volume":"12","author":"Tuffrey","year":"1981","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micinf.2017.01.008_bib13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-4320(92)90091-Q","article-title":"Endometritis and vaginal discharge in the sow","volume":"28","author":"De Winter","year":"1992","journal-title":"Anim Reprod Sci"},{"key":"10.1016\/j.micinf.2017.01.008_bib14","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1128\/iai.31.3.1161-1176.1981","article-title":"Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis","volume":"31","author":"Caldwell","year":"1981","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2017.01.008_bib15","doi-asserted-by":"crossref","first-page":"963","DOI":"10.4049\/jimmunol.115.4.963","article-title":"Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis: I. antigenic heterogeneity between C. trachomatis and C. psittaci","volume":"115","author":"Caldwell","year":"1975","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib16","series-title":"The laboratory swine","author":"Bollen","year":"2010"},{"key":"10.1016\/j.micinf.2017.01.008_bib17","doi-asserted-by":"crossref","first-page":"e003607","DOI":"10.1136\/bmjopen-2013-003607","article-title":"Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in an STI population: performances of the Presto CT-NG assay, the Lightmix Kit 480 HT CT\/NG and the COBAS Amplicor with urine specimens and urethral\/cervicovaginal samples","volume":"3","author":"Schuurs","year":"2013","journal-title":"BMJ Open"},{"key":"10.1016\/j.micinf.2017.01.008_bib18","doi-asserted-by":"crossref","first-page":"2441","DOI":"10.4049\/jimmunol.1103032","article-title":"CD4+ T Cells are necessary and sufficient to confer protection against Chlamydia trachomatis infection in the murine upper genital tract","volume":"189","author":"Gondek","year":"2012","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.dci.2016.01.006","article-title":"Characterization of cytological changes, IgA, IgG and IL-8 levels and pH value in the vagina of prepubertal and sexually mature Ellegaard G\u00f6ttingen minipigs during an estrous cycle","volume":"59","author":"Lorenzen","year":"2016","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib20","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1128\/IAI.68.7.4207-4216.2000","article-title":"Effects of estradiol and progesterone on susceptibility and early immune responses to Chlamydia trachomatis infection in the female reproductive tract","volume":"68","author":"Kaushic","year":"2000","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2017.01.008_bib21","first-page":"209","article-title":"Chlamydia trachomatis and oral contraceptive use: a quantitative review","volume":"68","author":"Cottingham","year":"1992","journal-title":"Genitourin Med"},{"key":"10.1016\/j.micinf.2017.01.008_bib22","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/S0002-9378(88)80189-3","article-title":"Estrogen enhances attachment of Chlamydia trachomatis to human endometrial epithelial cells in\u00a0vitro","volume":"159","author":"Maslow","year":"1998","journal-title":"Am J Obs Gynecol"},{"key":"10.1016\/j.micinf.2017.01.008_bib23","doi-asserted-by":"crossref","first-page":"4700","DOI":"10.1128\/IAI.71.8.4700-4710.2003","article-title":"Primary cultures of female swine genital epithelial cells in\u00a0vitro : a new approach for the study of hormonal modulation of Chlamydia infection","volume":"71","author":"Guseva","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2017.01.008_bib24","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1038\/nri3819","article-title":"The role of sex hormones in immune protection of the female reproductive tract","volume":"15","author":"Wira","year":"2015","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib25","first-page":"327","article-title":"Pulmonary recruitment of neutrophils and bacterial clearance in mice inoculated with aerosols of Pasteurella haemolytica or Staphylococcus aureus","volume":"49","author":"Mart\u00ednez-Burnes","year":"1985","journal-title":"Can J Comp Med"},{"key":"10.1016\/j.micinf.2017.01.008_bib26","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1111\/2049-632X.12164","article-title":"Innate immunity is sufficient for the clearance of Chlamydia trachomatis from the female mouse genital tract","volume":"72","author":"Sturdevant","year":"2014","journal-title":"Pathog Dis"},{"key":"10.1016\/j.micinf.2017.01.008_bib27","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nri3024","article-title":"Neutrophils in the activation and regulation of innate and adaptive immunity","volume":"11","author":"Mantovani","year":"2011","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.micinf.2017.01.008_bib28","series-title":"Janeway's immunobiology","author":"Murphy","year":"2011"},{"key":"10.1016\/j.micinf.2017.01.008_bib29","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1099\/jmm.0.024448-0","article-title":"Validation of the Chlamydia trachomatis genital challenge pig model for testing recombinant protein vaccines","volume":"60","author":"Schautteet","year":"2011","journal-title":"J\u00a0Med Microbiol"},{"key":"10.1016\/j.micinf.2017.01.008_bib30","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.tips.2013.03.006","article-title":"Targeting inflammation and wound healing by opioids","volume":"34","author":"Stein","year":"2013","journal-title":"Trends Pharmacol Sci"},{"key":"10.1016\/j.micinf.2017.01.008_bib31","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.4161\/hv.29683","article-title":"Immunity, immunopathology, and human vaccine development against sexually transmitted Chlamydia trachomatis","volume":"10","author":"Rey-Ladino","year":"2014","journal-title":"Hum Vaccines Immunother"}],"container-title":["Microbes and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457917300254?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457917300254?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:58:09Z","timestamp":1692680289000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1286457917300254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["S1286457917300254"],"URL":"https:\/\/doi.org\/10.1016\/j.micinf.2017.01.008","relation":{},"ISSN":["1286-4579"],"issn-type":[{"value":"1286-4579","type":"print"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Intrauterine inoculation of minipigs with Chlamydia trachomatis during diestrus establishes a longer lasting infection compared to vaginal inoculation during estrus","name":"articletitle","label":"Article Title"},{"value":"Microbes and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micinf.2017.01.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 The Author(s). Published by Elsevier Masson SAS on behalf of Institut Pasteur.","name":"copyright","label":"Copyright"}]}}