{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:15:01Z","timestamp":1790734501317,"version":"4.1.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"4-5","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":975,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770803"],"award-info":[{"award-number":["31770803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81861138047"],"award-info":[{"award-number":["81861138047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI127465"],"award-info":[{"award-number":["R01AI127465"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009666","name":"Salt Science Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009666","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbes and Infection"],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.1016\/j.micinf.2021.104794","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T01:45:08Z","timestamp":1612748708000},"page":"104794","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["The metaeffector MesI regulates the activity of the Legionella effector SidI through direct protein\u2013protein interactions"],"prefix":"10.1016","volume":"23","author":[{"given":"Alix","family":"McCloskey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4809-4686","authenticated-orcid":false,"given":"Kayla","family":"Perri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TaoTao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidong","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8890-6621","authenticated-orcid":false,"given":"Zhao-Qing","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micinf.2021.104794_bib1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrmicro1967","article-title":"The Legionella pneumophila replication vacuole: making a cosy niche inside host cells","volume":"7","author":"Isberg","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.micinf.2021.104794_bib2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1126\/science.279.5352.873","article-title":"Conjugative transfer by the virulence system of Legionella pneumophila","volume":"279","author":"Vogel","year":"1998","journal-title":"Science"},{"key":"10.1016\/j.micinf.2021.104794_bib3","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1073\/pnas.95.4.1669","article-title":"Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome","volume":"95","author":"Segal","year":"1998","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104794_bib4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1038\/nrmicro.2017.67","article-title":"Legionella and Coxiella effectors: strength in diversity and activity","volume":"15","author":"Qiu","year":"2017","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.micinf.2021.104794_bib5","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1016\/j.celrep.2017.10.088","article-title":"Positive and negative regulation of the master metabolic regulator mTORC1 by two families of Legionella pneumophila effectors","volume":"21","author":"De Leon","year":"2017","journal-title":"Cell Rep"},{"key":"10.1016\/j.micinf.2021.104794_bib6","doi-asserted-by":"crossref","first-page":"3221","DOI":"10.1073\/pnas.1820093116","article-title":"Legionella pneumophila translocated translation inhibitors are required for bacterial-induced host cell cycle arrest","volume":"116","author":"Sol","year":"2019","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104794_bib7","doi-asserted-by":"crossref","first-page":"3026","DOI":"10.1128\/JB.01798-07","article-title":"Lgt: a family of cytotoxic glucosyltransferases produced by Legionella pneumophila","volume":"190","author":"Belyi","year":"2008","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.micinf.2021.104794_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001289","article-title":"Secreted bacterial effectors that inhibit host protein synthesis are critical for induction of the innate immune response to virulent Legionella pneumophila","volume":"7","author":"Fontana","year":"2011","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.micinf.2021.104794_bib9","doi-asserted-by":"crossref","first-page":"6329","DOI":"10.4049\/jimmunol.1300100","article-title":"IL-1\u03b1 signaling initiates the inflammatory response to virulent Legionella pneumophila in\u00a0vivo","volume":"190","author":"Barry","year":"2013","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.micinf.2021.104794_bib10","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.chom.2019.01.006","article-title":"A\u00a0Legionella pneumophila kinase phosphorylates the Hsp70 chaperone family to inhibit eukaryotic protein synthesis","volume":"25","author":"Moss","year":"2019","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.micinf.2021.104794_bib11","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1111\/j.1462-5822.2009.01301.x","article-title":"Targeting eEF1A by a Legionella pneumophila effector leads to inhibition of protein synthesis and induction of host stress response","volume":"11","author":"Shen","year":"2009","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.micinf.2021.104794_bib12","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00853-19","article-title":"The Legionella pneumophila metaeffector Lpg2505 (MesI) regulates SidI-mediated translation inhibition and novel glycosyl hydrolase activity","volume":"88","author":"Joseph","year":"2020","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104794_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1001216","article-title":"Legionella metaeffector exploits host proteasome to temporally regulate cognate effector","volume":"6","author":"Kubori","year":"2010","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.micinf.2021.104794_bib14","doi-asserted-by":"crossref","first-page":"21212","DOI":"10.1073\/pnas.1114023109","article-title":"Legionella pneumophila regulates the small GTPase Rab1 activity by reversible phosphorylcholination","volume":"108","author":"Tan","year":"2011","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104794_bib15","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1038\/nature10307","article-title":"Legionella pneumophila SidD is a deAMPylase that modifies Rab1","volume":"475","author":"Tan","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.micinf.2021.104794_bib16","doi-asserted-by":"crossref","first-page":"893","DOI":"10.15252\/msb.20167381","article-title":"Diverse mechanisms of metaeffector activity in an intracellular bacterial pathogen, Legionella pneumophila","volume":"12","author":"Urbanus","year":"2016","journal-title":"Mol Syst Biol"},{"key":"10.1016\/j.micinf.2021.104794_bib17","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1126\/science.1207193","article-title":"De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila","volume":"333","author":"Neunuebel","year":"2011","journal-title":"Science"},{"key":"10.1016\/j.micinf.2021.104794_bib18","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1126\/science.aaw7446","article-title":"Bacterial pseudokinase catalyzes protein polyglutamylation to inhibit the SidE-family ubiquitin ligases","volume":"364","author":"Black","year":"2019","journal-title":"Science"},{"key":"10.1016\/j.micinf.2021.104794_bib19","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/s41586-019-1439-1","article-title":"Regulation of phosphoribosyl ubiquitination by a calmodulin-dependent glutamylase","volume":"572","author":"Gan","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.micinf.2021.104794_bib20","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.51162","article-title":"Protein polyglutamylation catalyzed by the bacterial calmodulin-dependent pseudokinase SidJ","volume":"8","author":"Sulpizio","year":"2019","journal-title":"Elife"},{"key":"10.1016\/j.micinf.2021.104794_bib21","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/s41586-019-1440-8","article-title":"Inhibition of bacterial ubiquitin ligases by SidJ-calmodulin catalysed glutamylation","volume":"572","author":"Bhogaraju","year":"2019","journal-title":"Nature"},{"key":"10.1016\/j.micinf.2021.104794_bib22","doi-asserted-by":"crossref","DOI":"10.15252\/embj.2019102806","article-title":"Legionella pneumophila regulates the activity of UBE2N by deamidase-mediated deubiquitination","volume":"39","author":"Gan","year":"2020","journal-title":"EMBO J"},{"key":"10.1016\/j.micinf.2021.104794_bib23","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1016\/j.celrep.2018.03.060","article-title":"Discovery of ubiquitin deamidases in the pathogenic arsenal of Legionella pneumophila","volume":"23","author":"Valleau","year":"2018","journal-title":"Cell Rep"},{"key":"10.1016\/j.micinf.2021.104794_bib24","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1038\/s41467-020-15645-7","article-title":"Structural insights into the mechanism and inhibition of transglutaminase-induced ubiquitination by the Legionella effector MavC","volume":"11","author":"Mu","year":"2020","journal-title":"Nat Commun"},{"key":"10.1016\/j.micinf.2021.104794_bib25","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1365-2958.1993.tb01092.x","article-title":"Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila","volume":"7","author":"Berger","year":"1993","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micinf.2021.104794_bib26","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1073\/pnas.0304916101","article-title":"Multiple substrates of the Legionella pneumophila Dot\/Icm system identified by interbacterial protein transfer","volume":"101","author":"Luo","year":"2004","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104794_bib27","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1128\/iai.65.6.2497-2501.1997","article-title":"Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis","volume":"65","author":"Merriam","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104794_bib28","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000822","article-title":"Inhibition of host vacuolar H+-ATPase activity by a Legionella pneumophila effector","volume":"6","author":"Xu","year":"2010","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.micinf.2021.104794_bib29","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/jb.177.14.4121-4130.1995","article-title":"Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter","volume":"177","author":"Guzman","year":"1995","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.micinf.2021.104794_bib30","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0378-1119(95)00584-1","article-title":"Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes","volume":"166","author":"Kovach","year":"1995","journal-title":"Gene"},{"key":"10.1016\/j.micinf.2021.104794_bib31","doi-asserted-by":"crossref","first-page":"3609","DOI":"10.1128\/iai.63.9.3609-3620.1995","article-title":"Association of Legionella pneumophila with the macrophage endoplasmic reticulum","volume":"63","author":"Swanson","year":"1995","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104794_bib32","doi-asserted-by":"crossref","first-page":"4370","DOI":"10.1128\/IAI.73.7.4370-4380.2005","article-title":"LidA, a translocated substrate of the Legionella pneumophila type IV secretion system, interferes with the early secretory pathway","volume":"73","author":"Derr\u00e9","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104794_bib33","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1073\/pnas.95.10.5752","article-title":"A\u00a0bacterial two-hybrid system based on a reconstituted signal transduction pathway","volume":"95","author":"Karimova","year":"1998","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104794_bib34","series-title":"Experiments in molecular genetics","author":"Miller","year":"1972"},{"key":"10.1016\/j.micinf.2021.104794_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004222","article-title":"The machinery at endoplasmic reticulum-plasma membrane contact sites contributes to spatial regulation of multiple Legionella effector proteins","volume":"10","author":"Hubber","year":"2014","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.micinf.2021.104794_bib36","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.1708553114","article-title":"Multiple Legionella pneumophila effector virulence phenotypes revealed through high-throughput analysis of targeted mutant libraries","volume":"114","author":"Shames","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.micinf.2021.104794_bib37","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1128\/IAI.01278-06","article-title":"The Legionella pneumophila effector SidJ is required for efficient recruitment of endoplasmic reticulum proteins to the bacterial phagosome","volume":"75","author":"Liu","year":"2007","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104794_bib38","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.ymeth.2012.07.018","article-title":"The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli","volume":"58","author":"Battesti","year":"2012","journal-title":"Methods"},{"key":"10.1016\/j.micinf.2021.104794_bib39","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/ng.3481","article-title":"Genomic analysis of 38 Legionella species identifies large and diverse effector repertoires","volume":"48","author":"Burstein","year":"2016","journal-title":"Nat Genet"},{"key":"10.1016\/j.micinf.2021.104794_bib40","doi-asserted-by":"crossref","first-page":"3239","DOI":"10.1128\/IAI.00552-13","article-title":"Poison domains block transit of translocated substrates via the Legionella pneumophila Icm\/Dot system","volume":"81","author":"Amyot","year":"2013","journal-title":"Infect Immun"}],"container-title":["Microbes and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457921000162?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457921000162?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T14:38:36Z","timestamp":1761143916000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1286457921000162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":40,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["S1286457921000162"],"URL":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104794","relation":{},"ISSN":["1286-4579"],"issn-type":[{"value":"1286-4579","type":"print"}],"subject":[],"published":{"date-parts":[[2021,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The metaeffector MesI regulates the activity of the Legionella effector SidI through direct protein\u2013protein interactions","name":"articletitle","label":"Article Title"},{"value":"Microbes and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104794","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Institut Pasteur. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"104794"}}