{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T15:20:39Z","timestamp":1770996039843,"version":"3.50.1"},"reference-count":44,"publisher":"Elsevier BV","issue":"4-5","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":975,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"FAPESP","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbes and Infection"],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.1016\/j.micinf.2021.104801","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T13:23:43Z","timestamp":1613049823000},"page":"104801","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii"],"prefix":"10.1016","volume":"23","author":[{"given":"Anna Erika","family":"Vieira de Araujo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Vidal","family":"Conde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haroldo Cid","family":"da Silva Junior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"de Almeida Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flavio Alves","family":"Lara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Chapeaurouge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Pauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiane Cassiolato","family":"Pires Hardoim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Caetano","family":"Martha Antunes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"D'Alincourt Carvalho-Assef","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Procopio","family":"Moreno Senna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micinf.2021.104801_bib1","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.1128\/AAC.00944-13","article-title":"A\u00a0mouse model of Acinetobacter baumannii-associated pneumonia using a clinically isolated hypervirulent strain","volume":"57","author":"Harris","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.micinf.2021.104801_bib2","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.micpath.2011.09.004","article-title":"Protection against Acinetobacter baumannii infection via its functional deprivation of biofilm associated protein (Bap)","volume":"51","author":"Fattahian","year":"2011","journal-title":"Microb Pathog"},{"key":"10.1016\/j.micinf.2021.104801_bib3","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1038\/nrmicro1789","article-title":"An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii","volume":"5","author":"Dijkshoorn","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.micinf.2021.104801_bib4","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/IAI.00741-10","article-title":"Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant Acinetobacter baumannii","volume":"79","author":"McConnell","year":"2011","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104801_bib5","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1002\/prca.201300133","article-title":"Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach","volume":"8","author":"Fajardo Bonin","year":"2014","journal-title":"PROTEOMICS\u2013Clinical Appl"},{"key":"10.1016\/j.micinf.2021.104801_bib6","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/2049-632X.12125","article-title":"Acinetobacter baumannii: evolution of a global pathogen","volume":"71","author":"Antunes","year":"2014","journal-title":"Pathog Dis"},{"key":"10.1016\/j.micinf.2021.104801_bib7","doi-asserted-by":"crossref","first-page":"4479","DOI":"10.1016\/j.vaccine.2015.07.031","article-title":"OmpW is a potential target for eliciting protective immunity against Acinetobacter baumannii infections","volume":"33","author":"Huang","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2021.104801_bib8","doi-asserted-by":"crossref","first-page":"20724","DOI":"10.1038\/srep20724","article-title":"Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii","volume":"6","author":"Huang","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.micinf.2021.104801_bib9","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.vaccine.2012.11.008","article-title":"Acinetobacter baumannii rOmpA vaccine dose alters immune polarization and immunodominant epitopes","volume":"31","author":"Lin","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2021.104801_bib10","doi-asserted-by":"crossref","first-page":"5451","DOI":"10.1007\/s00253-014-6339-6","article-title":"Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice","volume":"99","author":"Guan","year":"2015","journal-title":"Appl Microbiol Biotechnol"},{"issue":"10","key":"10.1016\/j.micinf.2021.104801_bib11","doi-asserted-by":"crossref","first-page":"1695","DOI":"10.1128\/CVI.05223-11","article-title":"Identification of the immunogenic outer membrane protein A (OmpA) antigen of Haemophilus parasuis using a proteomics approach and passive immunization of mice with monoclonal antibodies","volume":"18","author":"Tian","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.micinf.2021.104801_bib12","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1016\/j.vaccine.2009.12.071","article-title":"Identification and characterization of OmpA-like proteins as novel vaccine candidates for Leptospirosis","volume":"28","author":"Yan","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2021.104801_bib13","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1107\/S1399004714007482","article-title":"Structure of BamA, an essential factor in outer membrane protein biogenesis","volume":"70","author":"Albrecht","year":"2014","journal-title":"Acta Crystallogr Sect D Biol Crystallogr"},{"key":"10.1016\/j.micinf.2021.104801_bib14","doi-asserted-by":"crossref","first-page":"8038","DOI":"10.3390\/ijms13078038","article-title":"From evolution to pathogenesis: the link between \u03b2-barrel assembly machineries in the outer membrane of mitochondria and Gram-negative bacteria","volume":"13","author":"Jiang","year":"2012","journal-title":"Int J Mol Sci"},{"key":"10.1016\/j.micinf.2021.104801_bib15","first-page":"32","article-title":"In silico analysis of Acinetobacter baumannii outer membrane protein BamA as a potential immunogen","volume":"21","author":"Singh","year":"2014","journal-title":"Int J Pure Appl Sci Technol"},{"key":"10.1016\/j.micinf.2021.104801_bib16","doi-asserted-by":"crossref","first-page":"12411","DOI":"10.1038\/s41598-017-12789-3","article-title":"Immunoprotective potential of BamA, the outer membrane protein assembly factor, against MDR Acinetobacter baumannii","volume":"7","author":"Singh","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.micinf.2021.104801_bib17","doi-asserted-by":"crossref","first-page":"3692","DOI":"10.1073\/pnas.1800043115","article-title":"Monoclonal antibody targeting the \u03b2-barrel assembly machine of Escherichia coli is bactericidal","volume":"115","author":"Storek","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.micinf.2021.104801_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1046-5928(02)00641-1","article-title":"Practical considerations in refolding proteins from inclusion bodies","volume":"28","author":"Tsumoto","year":"2003","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.micinf.2021.104801_bib19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jviromet.2017.03.005","article-title":"Recombinant VP1 protein as a potential marker for the diagnosis of acute hepatitis A virus infection","volume":"245","author":"da Silva Junior","year":"2017","journal-title":"J\u00a0Virol Methods"},{"key":"10.1016\/j.micinf.2021.104801_bib20","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci Unit States Am"},{"key":"10.1016\/j.micinf.2021.104801_bib21","first-page":"336","article-title":"Determination of lethal and sublethal doses of Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus (MRSA) in murine models using a reduced number of animals","volume":"1","author":"Araujo","year":"2015","journal-title":"J\u00a0Exp Appl Anim Sci"},{"key":"10.1016\/j.micinf.2021.104801_bib22","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.diagmicrobio.2014.03.006","article-title":"Characterization of carbapenem-resistant Acinetobacter baumannii in Brazil (2008\u20132011): countrywide spread of OXA-23\u2013producing clones (CC15 and CC79)","volume":"79","author":"Chagas","year":"2014","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.micinf.2021.104801_bib23","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.micinf.2021.104801_bib24","doi-asserted-by":"crossref","first-page":"D590","DOI":"10.1093\/nar\/gks1219","article-title":"The SILVA ribosomal RNA gene database project: improved data processing and web-based tools","volume":"41","author":"Quast","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.micinf.2021.104801_bib25","doi-asserted-by":"crossref","first-page":"D643","DOI":"10.1093\/nar\/gkt1209","article-title":"The SILVA and \u201call-species Living Tree Project (LTP)\u201d taxonomic frameworks","volume":"42","author":"Yilmaz","year":"2014","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.micinf.2021.104801_bib26","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micinf.2021.104801_bib27","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1152\/physrev.00045.2009","article-title":"Gut microbiota in health and disease","volume":"90","author":"Sekirov","year":"2010","journal-title":"Physiol Rev"},{"key":"10.1016\/j.micinf.2021.104801_bib28","doi-asserted-by":"crossref","first-page":"897","DOI":"10.2174\/1389201014666131226123511","article-title":"First steps towards a vaccine against Acinetobacter baumannii","volume":"14","author":"Garcia-Quintanilla","year":"2014","journal-title":"Curr Pharmaceut Biotechnol"},{"key":"10.1016\/j.micinf.2021.104801_bib29","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetmic.2012.08.019","article-title":"The OmpA family of proteins: roles in bacterial pathogenesis and immunity","volume":"163","author":"Confer","year":"2013","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.micinf.2021.104801_bib30","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1093\/infdis\/jit386","article-title":"Association of the outer membrane protein Omp33 with fitness and virulence of Acinetobacter baumannii","volume":"208","author":"Smani","year":"2013","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104801_bib31","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1006\/bbrc.2002.6657","article-title":"The function of OmpA in Escherichia coli","volume":"292","author":"Wang","year":"2002","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.micinf.2021.104801_bib32","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1111\/j.1574-6976.2012.00344.x","article-title":"Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models","volume":"37","author":"McConnell","year":"2013","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.micinf.2021.104801_bib33","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1080\/21645515.2014.1004026","article-title":"Advances in aluminum hydroxide-based adjuvant research and its mechanism","volume":"11","author":"He","year":"2015","journal-title":"Hum Vaccines Immunother"},{"key":"10.1016\/j.micinf.2021.104801_bib34","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.imlet.2017.04.004","article-title":"The detection of antigenic determinants of Acinetobacter baumannii","volume":"186","author":"Tawfik","year":"2017","journal-title":"Immunol Lett"},{"key":"10.1016\/j.micinf.2021.104801_bib35","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00591-17","article-title":"The capsular polysaccharide of Acinetobacter baumannii is an obstacle for therapeutic passive immunization strategies","volume":"85","author":"Wang-Lin","year":"2017","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104801_bib36","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.1016\/j.vaccine.2008.07.100","article-title":"Targeting subcapsular antigens for prevention of Klebsiella pneumoniae infections","volume":"26","author":"Clements","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2021.104801_bib37","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1504\/IJIS.2012.051235","article-title":"Screening of novel epitopes in Klebsiella pneumoniae, as target for vaccine design","volume":"1","author":"El-Sayed","year":"2012","journal-title":"Int J Immunol Stud"},{"key":"10.1016\/j.micinf.2021.104801_bib38","doi-asserted-by":"crossref","DOI":"10.4081\/idr.2012.e33","article-title":"Development of a new trend conjugate vaccine for the prevention of Klebsiella pneumoniae","volume":"4","author":"Ahmad","year":"2012","journal-title":"Infect Dis Rep"},{"key":"10.1016\/j.micinf.2021.104801_bib39","doi-asserted-by":"crossref","first-page":"5089","DOI":"10.1007\/s00253-016-7467-y","article-title":"In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice","volume":"100","author":"Guan","year":"2016","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.micinf.2021.104801_bib40","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.3389\/fmicb.2017.01805","article-title":"Evaluation of recombinant multi-epitope outer membrane protein-based Klebsiella pneumoniae subunit vaccine in mouse model","volume":"8","author":"Babu","year":"2017","journal-title":"Front Microbiol"},{"key":"10.1016\/j.micinf.2021.104801_bib41","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.procbio.2015.05.001","article-title":"Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model","volume":"50","author":"Wang","year":"2015","journal-title":"Process Biochem"},{"key":"10.1016\/j.micinf.2021.104801_bib42","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1128\/AAC.01275-10","article-title":"Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence","volume":"55","author":"Tsai","year":"2011","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.micinf.2021.104801_bib43","doi-asserted-by":"crossref","first-page":"14683","DOI":"10.1038\/s41598-017-14972-y","article-title":"Combating virulence of Gram-negative bacilli by OmpA inhibition","volume":"7","author":"Vila-Farr\u00e9s","year":"2017","journal-title":"Sci Rep"},{"key":"10.1016\/j.micinf.2021.104801_bib44","first-page":"64","article-title":"Antibacterial monoclonal antibodies do not disrupt the intestinal microbiome or its function","author":"Jones-Nelson","year":"2020","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Microbes and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S128645792100023X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S128645792100023X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T14:38:44Z","timestamp":1761143924000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S128645792100023X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":44,"journal-issue":{"issue":"4-5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["S128645792100023X"],"URL":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104801","relation":{},"ISSN":["1286-4579"],"issn-type":[{"value":"1286-4579","type":"print"}],"subject":[],"published":{"date-parts":[[2021,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii","name":"articletitle","label":"Article Title"},{"value":"Microbes and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104801","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Institut Pasteur. Published by Elsevier Masson SAS.","name":"copyright","label":"Copyright"}],"article-number":"104801"}}