{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:40:36Z","timestamp":1792802436187,"version":"4.5.0"},"reference-count":31,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T00:00:00Z","timestamp":1630454400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":852,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312151\/2020-9"],"award-info":[{"award-number":["312151\/2020-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["421392\/2018-5"],"award-info":[{"award-number":["421392\/2018-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["442994\/2019-2"],"award-info":[{"award-number":["442994\/2019-2"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["CBB APQ-00766-18"],"award-info":[{"award-number":["CBB APQ-00766-18"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007375","name":"Pr\u00f3-Reitoria de Pesquisa, Universidade Federal de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007375","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbes and Infection"],"published-print":{"date-parts":[[2021,9]]},"DOI":"10.1016\/j.micinf.2021.104836","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:56:51Z","timestamp":1621385811000},"page":"104836","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":36,"title":["Unraveling Ascaris suum experimental infection in humans"],"prefix":"10.1016","volume":"23","author":[{"given":"Tha\u00eds Eloi da","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando S.","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-3295","authenticated-orcid":false,"given":"Luisa M.D.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro H.","family":"Gazzinelli-Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7079-3224","authenticated-orcid":false,"given":"Anderson C.","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise S.","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1496-5343","authenticated-orcid":false,"given":"Nathalia M.","family":"Resende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6490-9430","authenticated-orcid":false,"given":"Chiara C.","family":"Amorim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clara","family":"Gazzinelli-Guimar\u00e3es","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agostinho G.","family":"Viana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan M.","family":"Geiger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella C.","family":"Bartholomeu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo T.","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilian L.","family":"Bueno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micinf.2021.104836_bib1","series-title":"CDC - Ascariasis - General Information - Frequently Asked Questions","year":"2019"},{"key":"10.1016\/j.micinf.2021.104836_bib2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.micinf.2014.07.006","article-title":"Brazil's neglected tropical diseases: an overview and a report card","author":"Hotez","year":"2014","journal-title":"Microb Infect"},{"issue":"1","key":"10.1016\/j.micinf.2021.104836_bib3","doi-asserted-by":"crossref","first-page":"e177","DOI":"10.1371\/journal.pntd.0000177","article-title":"The giant anteater in the room: Brazil\u2019s neglected tropical diseases problem","volume":"2","author":"Hotez","year":"2008","journal-title":"PLoS Neglected Trop Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib4","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.pt.2003.10.002","article-title":"Soil-transmitted helminth infections: updating the global picture","author":"De Silva","year":"2003","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.micinf.2021.104836_bib5","series-title":"Are Ascaris lumbricoides and Ascaris suum a single species? Parasites and Vectors","author":"Leles","year":"2012"},{"key":"10.1016\/j.micinf.2021.104836_bib6","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.meegid.2012.01.012","article-title":"Ascariasis in people and pigs: new inferences from DNA analysis of worm populations","author":"Peng","year":"2012","journal-title":"Infect Genet Evol"},{"key":"10.1016\/j.micinf.2021.104836_bib7","series-title":"Impact of Ascaris suum in livestock","first-page":"363","author":"Thamsborg","year":"2013"},{"key":"10.1016\/j.micinf.2021.104836_bib8","first-page":"1355","article-title":"Ascaris lumbricoides, Ascaris suum, or \u201cAscaris lumbrisuum\u201d?","volume":"213","author":"Alves ElB da","year":"2016","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib9","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S1471-4922(00)01944-9","article-title":"The dangers of using single locus markers in parasite epidemiology: Ascaris as a case study","author":"Anderson","year":"2001","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.micinf.2021.104836_bib10","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1017\/S0031182006001521","article-title":"Recent insights into the epidemiology and genetics of Ascaris in China using molecular tools","author":"Peng","year":"2007","journal-title":"Parasitology"},{"key":"10.1016\/j.micinf.2021.104836_bib11","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1093\/infdis\/jiu193","article-title":"Molecular epidemiology of ascariasis: A global perspective on the transmission dynamics of ascaris in people and pigs","author":"Betson","year":"2014","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib12","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1007\/s00436-018-5931-6","article-title":"Molecular identification of Ascaris lumbricoides and Ascaris suum recovered from humans and pigs in Thailand, Lao PDR, and Myanmar","volume":"117","author":"Sadaow","year":"2018","journal-title":"Parasitol Res"},{"key":"10.1016\/j.micinf.2021.104836_bib13","doi-asserted-by":"crossref","first-page":"447","DOI":"10.7883\/yoken.63.447","article-title":"Ascariasis in Japan: is pig-derived Ascaris infecting humans?","volume":"63","author":"Arizono","year":"2010","journal-title":"Jpn J Infect Dis [Internet]"},{"key":"10.1016\/j.micinf.2021.104836_bib14","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1089\/vbz.2018.2306","article-title":"Molecular confirmation of Ascaris suum\u00a0: further investigation into the zoonotic origin of infection in an 8-year-old boy with loeffler syndrome","volume":"18","author":"Avery","year":"2018","journal-title":"Vector-Borne Zoonotic Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib15","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.ijpara.2013.02.009","article-title":"Parasitological and immunological aspects of early Ascaris spp. infection in mice","volume":"43","author":"Gazzinelli-Guimar\u00e3es","year":"2013","journal-title":"Int J Parasitol [Internet]"},{"key":"10.1016\/j.micinf.2021.104836_bib16","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.1016\/j.vaccine.2007.12.031","article-title":"Direct effect of Plasmodium vivax recombinant vaccine candidates AMA-1 and MSP-119 on the innate immune response","volume":"26","author":"Bueno","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.micinf.2021.104836_bib17","doi-asserted-by":"crossref","first-page":"W566","DOI":"10.1093\/nar\/gkv468","article-title":"ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap","volume":"43","author":"Metsalu","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.micinf.2021.104836_bib18","doi-asserted-by":"crossref","first-page":"227","DOI":"10.2223\/JPED.1024","article-title":"Asthma and ascariasis in children aged two to ten living in a low income suburb","volume":"79","author":"Silva","year":"2003","journal-title":"J\u00a0Pediatr (Rio J) - Jornal de Pediatria"},{"key":"10.1016\/j.micinf.2021.104836_bib19","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1111\/j.1365-2249.1993.tb05981.x","article-title":"Ascaris reinfection of slum children: relation with the IgE response","volume":"94","author":"Hagel","year":"2008","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.micinf.2021.104836_bib20","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1128\/IAI.67.2.484-489.1999","article-title":"Natural immunity to Ascaris lumbricoides associated with immunoglobulin E antibody to ABA-1 allergen and inflammation indicators in children","volume":"67","author":"McSharry","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.micinf.2021.104836_bib21","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1017\/S0031182000060893","article-title":"Immunoepidemiology of Ascaris lumbricoides: relationships between antibody specificities, exposure and infection in a human community","volume":"104","author":"Haswell-Elkins","year":"1992","journal-title":"Parasitology"},{"key":"10.1016\/j.micinf.2021.104836_bib22","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1111\/j.1365-3024.1989.tb00925.x","article-title":"The antibody recognition profiles of humans naturally infected with Ascaris lumbricoides","volume":"11","author":"Haswell\u2013Elkins","year":"1989","journal-title":"Parasite Immunol"},{"issue":"Pt 3","key":"10.1016\/j.micinf.2021.104836_bib23","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1017\/S0031182000081944","article-title":"Antibody isotype responses to antigens of Ascaris lumbricoides in a case-control study of persistently heavily infected Bangladeshi children","volume":"111","author":"Palmer","year":"1995","journal-title":"Parasitology"},{"key":"10.1016\/j.micinf.2021.104836_bib24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1365-3024.2005.00753.x","article-title":"Immuno-epidemiology of Ascaris lumbricoides infection in a high transmission community: antibody responses and their impact on current and future infection intensity","volume":"27","author":"King","year":"2005","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.micinf.2021.104836_bib25","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1086\/423944","article-title":"Atopic phenotype is an important determinant of immunoglobulin E\u2013mediated inflammation and expression of T helper cell type 2 cytokines to Ascaris antigens in children exposed to ascariasis","volume":"190","author":"Cooper","year":"2004","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib26","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1086\/425014","article-title":"T\u00a0helper cell type 2 responsiveness predicts future susceptibility to gastrointestinal nematodes in humans","volume":"190","author":"Jackson","year":"2004","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib27","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1086\/379370","article-title":"Th2 cytokines are associated with reduced worm burdens in a human intestinal helminth infection","volume":"188","author":"Turner","year":"2003","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib28","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1086\/339005","article-title":"Age and infection intensity\u2013dependent cytokine and antibody production in human trichuriasis: the importance of IgE","volume":"185","author":"Faulkner","year":"2002","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pntd.0004382","article-title":"Multiple exposures to Ascaris suum induce tissue injury and mixed Th2\/Th17 immune response in mice","volume":"10","author":"Nogueira","year":"2016","journal-title":"PLoS Neglected Trop Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib30","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1093\/infdis\/jix585","article-title":"Ascaris suum infection downregulates inflammatory pathways in the pig intestine in\u00a0vivo and in human dendritic cells in\u00a0vitro","volume":"217","author":"Midttun","year":"2018","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micinf.2021.104836_bib31","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1111\/imm.13151","article-title":"Body fluid from the parasitic worm Ascaris suum inhibits broad-acting pro-inflammatory programs in dendritic cells","volume":"159","author":"Arora","year":"2020","journal-title":"Immunology"}],"container-title":["Microbes and Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457921000587?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1286457921000587?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T14:40:08Z","timestamp":1761144008000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1286457921000587"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["S1286457921000587"],"URL":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104836","relation":{},"ISSN":["1286-4579"],"issn-type":[{"value":"1286-4579","type":"print"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Unraveling Ascaris suum experimental infection in humans","name":"articletitle","label":"Article Title"},{"value":"Microbes and Infection","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micinf.2021.104836","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Published by Elsevier Masson SAS on behalf of Institut Pasteur.","name":"copyright","label":"Copyright"}],"article-number":"104836"}}