{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:47:33Z","timestamp":1761058053725},"reference-count":46,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2004,10]]},"DOI":"10.1016\/j.micpath.2004.07.003","type":"journal-article","created":{"date-parts":[[2004,9,21]],"date-time":"2004-09-21T18:24:36Z","timestamp":1095791076000},"page":"205-213","source":"Crossref","is-referenced-by-count":9,"title":["Identification of Francisella tularensis genes encoding exported membrane-associated proteins using TnphoA mutagenesis of a genomic library"],"prefix":"10.1016","volume":"37","author":[{"suffix":"Jr.","given":"Robert D.","family":"Gilmore","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rendi","family":"Murphree Bacon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L.","family":"Sviat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannine M.","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott W.","family":"Bearden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2004.07.003_bib1","doi-asserted-by":"crossref","first-page":"2763","DOI":"10.1001\/jama.285.21.2763","article-title":"Tularemia as a biological weapon: medical and public health management","volume":"285","author":"Dennis","year":"2001","journal-title":"JAMA"},{"key":"10.1016\/j.micpath.2004.07.003_bib2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/CMR.15.4.631-646.2002","volume":"15","author":"Ellis","year":"2002","journal-title":"Tularemia. Clin Microbiol Rev"},{"key":"10.1016\/j.micpath.2004.07.003_bib3","first-page":"349","article-title":"Life and death of an intracellular pathogen: Francisella tularensis and the macrophage","volume":"60","author":"Fortier","year":"1994","journal-title":"Immunol Ser"},{"key":"10.1016\/j.micpath.2004.07.003_bib4","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1128\/IAI.63.4.1478-1483.1995","article-title":"Growth of Francisella tularensis LVS in macrophages: the acidic intracellular compartment provides essential iron required for growth","volume":"63","author":"Fortier","year":"1995","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2004.07.003_bib5","doi-asserted-by":"crossref","first-page":"3291","DOI":"10.1128\/IAI.59.9.3291-3296.1991","article-title":"Growth of Francisella spp. in rodent macrophages","volume":"59","author":"Anthony","year":"1991","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2004.07.003_bib6","doi-asserted-by":"crossref","first-page":"5940","DOI":"10.1128\/IAI.71.10.5940-5950.2003","article-title":"An attenuated strain of the facultative intracellular bacterium Francisella tularensis can escape the phagosome of monocytic cells","volume":"71","author":"Golovliov","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2004.07.003_bib7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S1286-4579(02)00084-9","article-title":"Innate and adaptive immune responses to an intracellular bacterium. Francisella tularensis live vaccine strain","volume":"5","author":"Elkins","year":"2003","journal-title":"Microbes Infect"},{"key":"10.1016\/j.micpath.2004.07.003_bib8","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/S1369-5274(03)00002-X","article-title":"Virulence determinants and protective antigens of Francisella tularensis","volume":"6","author":"Sjostedt","year":"2003","journal-title":"Curr Opin Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib9","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/j.1365-2958.1996.tb02524.x","article-title":"Phase variation in Francisella tularensis affecting intracellular growth, lipopolysaccharide antigenicity and nitric oxide production","volume":"20","author":"Cowley","year":"1996","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib10","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1574-6968.2000.tb08874.x","article-title":"Isolation and characterization of Francisella novicida mutants defective in lipopolysaccharide biosynthesis","volume":"182","author":"Cowley","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2004.07.003_bib11","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1128\/JCM.29.7.1407-1412.1991","article-title":"Production and characterization of monoclonal antibodies directed against the lipopolysaccharide of Francisella tularensis","volume":"29","author":"Fulop","year":"1991","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib12","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1128\/IAI.56.5.1194-1202.1988","article-title":"A capsule-deficient mutant of Francisella tularensis LVS exhibits enhanced sensitivity to killing by serum but diminished sensitivity to killing by polymorphonuclear leukocytes","volume":"56","author":"Sandstrom","year":"1988","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2004.07.003_bib13","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1574-695X.1996.tb00246.x","article-title":"Francisella tularensis resistance to bactericidal action of normal human serum","volume":"13","author":"Sorokin","year":"1996","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib14","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1128\/MMBR.57.1.50-108.1993","article-title":"The complete general secretory pathway in gram-negative bacteria","volume":"57","author":"Pugsley","year":"1993","journal-title":"Microbiol Rev"},{"key":"10.1016\/j.micpath.2004.07.003_bib15","doi-asserted-by":"crossref","first-page":"8129","DOI":"10.1073\/pnas.82.23.8129","article-title":"TnphoA: a transposon probe for protein export signals","volume":"82","author":"Manoil","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.micpath.2004.07.003_bib16","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1128\/jb.172.2.515-518.1990","article-title":"Alkaline phosphatase fusions: sensors of subcellular location","volume":"172","author":"Manoil","year":"1990","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib17","doi-asserted-by":"crossref","first-page":"5107","DOI":"10.1073\/pnas.82.15.5107","article-title":"Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion","volume":"82","author":"Hoffman","year":"1985","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.micpath.2004.07.003_bib18","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1128\/jb.169.4.1663-1669.1987","article-title":"Use of TnphoA to detect genes for exported proteins in Escherichia coli: identification of the plasmid-encoded gene for a periplasmic acid phosphatase","volume":"169","author":"Boquet","year":"1987","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib19","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1111\/j.1365-2958.1992.tb01355.x","article-title":"PhoA gene fusions in Legionella pneumophila generated in vivo using a new transposon MudphoA","volume":"6","author":"Albano","year":"1992","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib20","doi-asserted-by":"crossref","first-page":"5830","DOI":"10.1128\/jb.171.11.5830-5839.1989","article-title":"Construction, expression, and localization of a CycA\u2237PhoA fusion protein in Rhodobacter sphaeroides and Escherichia coli","volume":"171","author":"Varga","year":"1989","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib21","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1128\/JB.180.5.1135-1147.1998","article-title":"An ABC transporter system of Yersinia pestis allows utilizaton of chelated iron by Escherichia coli SAB11","volume":"180","author":"Bearden","year":"1998","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib22","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1111\/j.1365-2958.1989.tb00226.x","article-title":"Expression and phase variation of gonococcal P.II genes in Escherichia coli involves ribosomal frameshifting and slipped-strand mispairing","volume":"3","author":"Belland","year":"1989","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib23","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1111\/j.1365-2958.1991.tb02086.x","article-title":"Identification of Treponema pallidum subspecies pallidum genes encoding signal peptides and membrane-spanning sequences using a novel alkaline phosphatase expression vector","volume":"5","author":"Blanco","year":"1991","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib24","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1574-6968.1998.tb13312.x","article-title":"Identification and sequence analysis of Treponema pallidum tprJ, a member of a polymorphic multigene family","volume":"169","author":"Stamm","year":"1998","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2004.07.003_bib25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1111\/j.1365-2958.1995.mmi_18020283.x","article-title":"Identification of Mycoplasma membrane proteins by systematic TnphoA mutagenesis of a recombinant library","volume":"18","author":"Cleavinger","year":"1995","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib26","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1128\/jb.177.1.59-65.1995","article-title":"Identification of Mycobacterium tuberculosis DNA sequences encoding exported proteins by using phoA gene fusions","volume":"177","author":"Lim","year":"1995","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib27","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/0022-2836(87)90650-4","article-title":"The use of transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus. Analysis of osmotically regulated genes in Escherichia coli","volume":"195","author":"Gutierrez","year":"1987","journal-title":"J Mol Biol"},{"key":"10.1016\/j.micpath.2004.07.003_bib28","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1002\/1615-9861(200207)2:7<857::AID-PROT857>3.0.CO;2-L","article-title":"Mapping of immunoreactive antigens of Francisella tularensis live vaccine strain","volume":"2","author":"Havlasova","year":"2002","journal-title":"Proteomics"},{"key":"10.1016\/j.micpath.2004.07.003_bib29","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.1128\/JCM.30.9.2256-2264.1992","article-title":"Cell-mediated and humoral immune responses induced by scarification vaccination of human volunteers with a new lot of the live vaccine strain of Francisella tularensis","volume":"30","author":"Waag","year":"1992","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib30","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1128\/JCM.26.3.433-437.1988","article-title":"Agglutinins and antibodies to Francisella tularensis outer membrane antigens in the early diagnosis of disease during an outbreak of tularemia","volume":"26","author":"Bevanger","year":"1988","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib31","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/clinids\/11.3.440","article-title":"Nature of protective immunity to Francisella tularensis","volume":"11","author":"Tarnvik","year":"1989","journal-title":"Rev Infect Dis"},{"key":"10.1016\/j.micpath.2004.07.003_bib32","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1128\/JCM.25.4.641-644.1987","article-title":"Immunospecific T-lymphocyte stimulation by membrane proteins from Francisella tularensis","volume":"25","author":"Sandstrom","year":"1987","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib33","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/j.1574-6968.1993.tb06407.x","article-title":"Analysis of a cloned Francisella tularensis outer membrane protein gene and expression in attenuated Salmonella typhimurium","volume":"111","author":"Leslie","year":"1993","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2004.07.003_bib34","doi-asserted-by":"crossref","first-page":"311","DOI":"10.4049\/jimmunol.145.1.311","article-title":"Nucleotide sequence and T cell epitopes of a membrane protein of Francisella tularensis","volume":"145","author":"Sjostedt","year":"1990","journal-title":"J Immunol"},{"issue":"Pt 12","key":"10.1016\/j.micpath.2004.07.003_bib35","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1099\/13500872-140-12-3309","article-title":"Serum-sensitive mutation of Francisella novicida: association with an ABC transporter gene","volume":"140","author":"Mdluli","year":"1994","journal-title":"Microbiology"},{"key":"10.1016\/j.micpath.2004.07.003_bib36","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1046\/j.1365-2958.1998.00926.x","article-title":"MglA and MglB are required for the intramacrophage growth of Francisella novicida","volume":"29","author":"Baron","year":"1998","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib37","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1574-6968.2002.tb11369.x","article-title":"The identification of five genetic loci of Francisella novicida associated with intracellular growth","volume":"215","author":"Gray","year":"2002","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2004.07.003_bib38","doi-asserted-by":"crossref","first-page":"4246","DOI":"10.1073\/pnas.0307690101","article-title":"MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survival","volume":"101","author":"Lauriano","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.micpath.2004.07.003_bib39","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1128\/IAI.65.6.2183-2189.1997","article-title":"Identification of proteins of Francisella tularensis induced during growth in macrophages and cloning of the gene encoding a prominently induced 23-kilodalton protein","volume":"65","author":"Golovliov","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2004.07.003_bib40","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0378-1097(03)00313-6","article-title":"A method for allelic replacement in Francisella tularensis","volume":"222","author":"Golovliov","year":"2003","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2004.07.003_bib41","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1111\/j.1365-2958.1990.tb00540.x","article-title":"Beta-lactamase as a probe of membrane protein assembly and protein export","volume":"4","author":"Broome-Smith","year":"1990","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/MMBR.62.1.1-34.1998","article-title":"Major facilitator superfamily","volume":"62","author":"Pao","year":"1998","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1016\/j.micpath.2004.07.003_bib43","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1128\/JB.180.7.1831-1840.1998","article-title":"Acquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae","volume":"180","author":"Hakenbeck","year":"1998","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2004.07.003_bib44","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1128\/AAC.48.5.1848-1855.2004","article-title":"Biochemical characterization of Streptococcus pneumoniae penicillin-binding protein 2b and its implication in beta-lactam resistance","volume":"48","author":"Pagliero","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.micpath.2004.07.003_bib45","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1046\/j.1365-2672.2001.01499.x","article-title":"Preliminary analysis and annotation of the partial genome sequence of Francisella tularensis strain Schu 4","volume":"91","author":"Prior","year":"2001","journal-title":"J Appl Microbiol"},{"key":"10.1016\/j.micpath.2004.07.003_bib46","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1128\/JCM.22.2.212-215.1985","article-title":"Antimicrobial susceptibility testing of Francisella tularensis with a modified Mueller\u2013Hinton broth","volume":"22","author":"Baker","year":"1985","journal-title":"J Clin Microbiol"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401004000865?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401004000865?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,4,29]],"date-time":"2023-04-29T10:18:08Z","timestamp":1682763488000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401004000865"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["S0882401004000865"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2004.07.003","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2004,10]]}}}