{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:59:56Z","timestamp":1791338396669,"version":"4.3.1"},"reference-count":46,"publisher":"Elsevier BV","issue":"4","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2008,10]]},"DOI":"10.1016\/j.micpath.2008.06.006","type":"journal-article","created":{"date-parts":[[2008,7,17]],"date-time":"2008-07-17T00:32:58Z","timestamp":1216254778000},"page":"273-281","source":"Crossref","is-referenced-by-count":26,"title":["A novel prfA mutation that promotes Listeria monocytogenes cytosol entry but reduces bacterial spread and cytotoxicity"],"prefix":"10.1016","volume":"45","author":[{"given":"Maurine D.","family":"Miner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary C.","family":"Port","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.G. Archie","family":"Bouwer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer C.","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2008.06.006_bib1","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1001\/jama.261.9.1313","article-title":"Listeriosis","volume":"261","author":"Gellin","year":"1989","journal-title":"JAMA"},{"key":"10.1016\/j.micpath.2008.06.006_bib2","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","article-title":"Listeria pathogenesis and molecular virulence determinants","volume":"14","author":"Vazquez-Boland","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.micpath.2008.06.006_bib3","first-page":"4","article-title":"Listeria \u2013 review of epidemiology and pathogenesis","volume":"40","author":"Ramaswamy","year":"2007","journal-title":"J Microbiol Immunol Infect"},{"key":"10.1016\/j.micpath.2008.06.006_bib4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1542\/pir.25-5-151","volume":"25","author":"Posfay-Barbe","year":"2004","journal-title":"Listeriosis. Pediatr Rev"},{"key":"10.1016\/j.micpath.2008.06.006_bib5","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1078\/1438-4221-00111","article-title":"Regulation of virulence genes in Listeria","volume":"291","author":"Kreft","year":"2001","journal-title":"Int J Med Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib6","first-page":"187","article-title":"Molecular and genetic determinants of the Listeria monocytogenes infectious process","volume":"192","author":"Sheehan","year":"1994","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.micpath.2008.06.006_bib7","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1146\/annurev.micro.57.030502.090934","article-title":"Molecular determinants of Listeria monocytogenes virulence","volume":"58","author":"Dussurget","year":"2004","journal-title":"Annu Rev Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib8","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nrmicro1413","article-title":"Listeria monocytogenes: a multifaceted model","volume":"4","author":"Hamon","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib9","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1128\/IAI.74.5.2505-2512.2006","article-title":"How the bacterial pathogen Listeria monocytogenes mediates the switch from environmental Dr. Jekyll to pathogenic Mr.\u00a0Hyde","volume":"74","author":"Gray","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib10","doi-asserted-by":"crossref","first-page":"8336","DOI":"10.1073\/pnas.87.21.8336","article-title":"Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of Listeria monocytogenes","volume":"87","author":"Leimeister-Wachter","year":"1990","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.micpath.2008.06.006_bib11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1111\/j.1574-6968.1989.tb03591.x","article-title":"Mutations affecting hemolysin production in Listeria monocytogenes located outside the listeriolysin gene","volume":"53","author":"Leimeister-Wachter","year":"1989","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2008.06.006_bib12","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1128\/jb.174.2.568-574.1992","article-title":"Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene","volume":"174","author":"Chakraborty","year":"1992","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2008.06.006_bib13","series-title":"Listeria monocytogenes: pathogenesis and host response","first-page":"139","article-title":"Regulation of Listeria monocytogenes virulence genes","author":"Miner","year":"2007"},{"key":"10.1016\/j.micpath.2008.06.006_bib14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/j.1365-2958.1994.tb00409.x","article-title":"The virulence regulator protein of Listeria ivanovii is highly homologous to PrfA from Listeria monocytogenes and both belong to the Crp-Fnr family of transcription regulators","volume":"13","author":"Lampidis","year":"1994","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib15","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1006\/jmbi.1999.3161","article-title":"Transcription activation by catabolite activator protein (CAP)","volume":"293","author":"Busby","year":"1999","journal-title":"J Mol Biol"},{"key":"10.1016\/j.micpath.2008.06.006_bib16","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/S0168-6445(03)00066-4","article-title":"Phylogeny of the bacterial superfamily of Crp-Fnr transcription regulators: exploiting the metabolic spectrum by controlling alternative gene programs","volume":"27","author":"Korner","year":"2003","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.micpath.2008.06.006_bib17","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/S0092-8674(85)80055-6","article-title":"Sites of allosteric shift in the structure of the cyclic AMP receptor protein","volume":"41","author":"Garges","year":"1985","journal-title":"Cell"},{"key":"10.1016\/j.micpath.2008.06.006_bib18","doi-asserted-by":"crossref","first-page":"8072","DOI":"10.1016\/S0021-9258(18)68443-1","article-title":"Arginine substituted for leucine at position 195 produces a cyclic AMP-independent form of the Escherichia coli cyclic AMP receptor protein","volume":"263","author":"Harman","year":"1988","journal-title":"J Biol Chem"},{"key":"10.1016\/j.micpath.2008.06.006_bib19","doi-asserted-by":"crossref","first-page":"6265","DOI":"10.1128\/JB.186.18.6265-6276.2004","article-title":"A novel mutation within the central Listeria monocytogenes regulator PrfA that results in constitutive expression of virulence gene products","volume":"186","author":"Wong","year":"2004","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2008.06.006_bib20","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1128\/jb.179.5.1533-1540.1997","article-title":"A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes","volume":"179","author":"Ripio","year":"1997","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2008.06.006_bib21","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1046\/j.1365-2958.2003.03534.x","article-title":"Isolation of Listeria monocytogenes mutants with high-level in vitro expression of host cytosol-induced gene products","volume":"48","author":"Shetron-Rama","year":"2003","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib22","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1111\/j.1365-2958.2004.04052.x","article-title":"New Listeria monocytogenes prfA* mutants, transcriptional properties of PrfA* proteins and structure\u2013function of the virulence regulator PrfA","volume":"52","author":"Vega","year":"2004","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib23","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/0923-2508(96)84712-7","article-title":"Transcriptional activation of virulence genes in wild-type strains of Listeria monocytogenes in response to a change in the extracellular medium composition","volume":"147","author":"Ripio","year":"1996","journal-title":"Res Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib24","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1128\/IAI.61.6.2537-2544.1993","article-title":"Regulation of the prfA transcriptional activator of Listeria monocytogenes: multiple promoter elements contribute to intracellular growth and cell-to-cell spread","volume":"61","author":"Freitag","year":"1993","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib25","doi-asserted-by":"crossref","first-page":"4177","DOI":"10.1128\/JB.184.15.4177-4186.2002","article-title":"Construction, characterization, and use of two Listeria monocytogenes site-specific phage integration vectors","volume":"184","author":"Lauer","year":"2002","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2008.06.006_bib26","doi-asserted-by":"crossref","first-page":"11890","DOI":"10.1073\/pnas.90.24.11890","article-title":"Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells","volume":"90","author":"Brundage","year":"1993","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.micpath.2008.06.006_bib27","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1128\/IAI.58.11.3770-3778.1990","article-title":"Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread","volume":"58","author":"Sun","year":"1990","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib28","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1038\/357257a0","article-title":"The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization","volume":"357","author":"Theriot","year":"1992","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2008.06.006_bib29","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1574-6968.1997.tb10474.x","article-title":"Neomycin- and spectinomycin-resistance replacement vectors for Bacillus subtilis","volume":"153","author":"Chary","year":"1997","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2008.06.006_bib30","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1128\/IAI.70.3.1087-1096.2002","article-title":"Intracellular induction of Listeria monocytogenes actA expression","volume":"70","author":"Shetron-Rama","year":"2002","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib31","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1128\/IAI.73.4.1917-1926.2005","article-title":"Pleiotropic enhancement of bacterial pathogenesis resulting from the constitutive activation of the Listeria monocytogenes regulatory factor PrfA","volume":"73","author":"Mueller","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib32","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1128\/IAI.1.4.363-372.1970","article-title":"Biochemical and immunological effects of Listeria monocytogenes hemolysin","volume":"1","author":"Kingdon","year":"1970","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib33","doi-asserted-by":"crossref","first-page":"5522","DOI":"10.1073\/pnas.86.14.5522","article-title":"Intracellular methicillin selection of Listeria monocytogenes mutants unable to replicate in a macrophage cell line","volume":"86","author":"Camilli","year":"1989","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.micpath.2008.06.006_bib34","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1046\/j.1462-5822.2003.00348.x","article-title":"Evidence implicating the 5\u2032 untranslated region of Listeria monocytogenes actA in the regulation of bacterial actin-based motility","volume":"6","author":"Wong","year":"2004","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib35","series-title":"Plasmids: A Practical Approach","first-page":"79","article-title":"Plasmid vectors for recovering and exploiting Tn917 transpositions in Bacillus and other Gram-positive bacteria","author":"Youngman","year":"1987"},{"key":"10.1016\/j.micpath.2008.06.006_bib36","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1128\/IAI.67.1.131-139.1999","article-title":"Expression of listeriolysin O and ActA by intracellular and extracellular Listeria monocytogenes","volume":"67","author":"Moors","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib37","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1084\/jem.167.4.1459","article-title":"Role of hemolysin for the intracellular growth of Listeria monocytogenes","volume":"167","author":"Portnoy","year":"1988","journal-title":"J Exp Med"},{"key":"10.1016\/j.micpath.2008.06.006_bib38","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1126\/science.290.5493.992","article-title":"A PEST-like sequence in listeriolysin O essential for Listeria monocytogenes pathogenicity","volume":"290","author":"Decatur","year":"2000","journal-title":"Science"},{"key":"10.1016\/j.micpath.2008.06.006_bib39","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.1128\/IAI.59.12.4641-4646.1991","article-title":"Production and characterization of neutralizing and nonneutralizing monoclonal antibodies against listeriolysin O","volume":"59","author":"Nato","year":"1991","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib40","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/PL00008633","article-title":"Differential expression of Listeria monocytogenes virulence genes in mammalian host cells","volume":"261","author":"Bubert","year":"1999","journal-title":"Mol Gen Genet"},{"key":"10.1016\/j.micpath.2008.06.006_bib41","doi-asserted-by":"crossref","first-page":"1844","DOI":"10.1128\/IAI.67.4.1844-1852.1999","article-title":"Examination of Listeria monocytogenes intracellular gene expression by using the green fluorescent protein of Aequorea victoria","volume":"67","author":"Freitag","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2008.06.006_bib42","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0378-1119(95)00402-R","article-title":"Construction of gusA transcriptional fusion vectors for Bacillus subtilis and their utilization for studies of spore formation","volume":"163","author":"Karow","year":"1995","journal-title":"Gene"},{"key":"10.1016\/j.micpath.2008.06.006_bib43","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1111\/j.1365-2958.2004.04003.x","article-title":"Negative control of Listeria monocytogenes virulence genes by a diffusible autorepressor","volume":"52","author":"Ermolaeva","year":"2004","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib44","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1083\/jcb.200201081","article-title":"The Listeria monocytogenes hemolysin has an acidic pH optimum to compartmentalize activity and prevent damage to infected host cells","volume":"156","author":"Glomski Ij","year":"2002","journal-title":"J Cell Biol"},{"key":"10.1016\/j.micpath.2008.06.006_bib45","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1365-2958.2005.04561.x","article-title":"The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif","volume":"56","author":"Eiting","year":"2005","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2008.06.006_bib46","doi-asserted-by":"crossref","first-page":"5608","DOI":"10.1128\/IAI.62.12.5608-5613.1994","article-title":"Characterization of Listeria monocytogenes pathogenesis in a strain expressing perfringolysin O in place of listeriolysin O","volume":"62","author":"Jones","year":"1994","journal-title":"Infect Immun"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401008000855?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401008000855?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,9,13]],"date-time":"2021-09-13T05:31:29Z","timestamp":1631511089000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401008000855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["S0882401008000855"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2008.06.006","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2008,10]]}}}