{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T21:27:57Z","timestamp":1771709277242,"version":"3.50.1"},"reference-count":32,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2010,5]]},"DOI":"10.1016\/j.micpath.2010.01.005","type":"journal-article","created":{"date-parts":[[2010,1,25]],"date-time":"2010-01-25T07:06:53Z","timestamp":1264403213000},"page":"191-195","source":"Crossref","is-referenced-by-count":16,"title":["A Yersinia pestis guaBA mutant is attenuated in virulence and provides protection against plague in a mouse model of infection"],"prefix":"10.1016","volume":"48","author":[{"given":"Petra C.F.","family":"Oyston","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Mellado-Sanchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela F.","family":"Pasetti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James P.","family":"Nataro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen S.","family":"Atkins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2010.01.005_bib1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.10.1.35","article-title":"Yersinia pestis \u2013 etiologic agent of plague","volume":"10","author":"Perry","year":"1997","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.micpath.2010.01.005_bib2","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1001\/jama.283.17.2281","article-title":"Plague as a biological weapon: medical and public health management. Working Group on Civilian Biodefense","volume":"283","author":"Inglesby","year":"2000","journal-title":"JAMA"},{"issue":"Suppl. 8","key":"10.1016\/j.micpath.2010.01.005_bib3","article-title":"Plague immunization. I. Past and present trends","volume":"129","author":"Meyer","year":"1974","journal-title":"J Infect Dis"},{"key":"10.1016\/j.micpath.2010.01.005_bib4","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1016\/0264-410X(95)00090-N","article-title":"A comparison of plague vaccine, USP and EV76 vaccine induced protection against Yersinia pestis in a murine model","volume":"13","author":"Russell","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2010.01.005_bib5","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/S0264-410X(96)00303-9","article-title":"A sub-unit vaccine elicits IgG in serum, spleen cell cultures and bronchial washings and protects immunized animals against pneumonic plague","volume":"15","author":"Williamson","year":"1997","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2010.01.005_bib6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1111\/j.1574-695X.1995.tb00196.x","article-title":"A new improved sub-unit vaccine for plague: the basis of protection","volume":"12","author":"Williamson","year":"1995","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.micpath.2010.01.005_bib7","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1016\/S0264-410X(98)80110-2","article-title":"Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine","volume":"16","author":"Heath","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2010.01.005_bib8","first-page":"408","article-title":"An unusual strain of Pasteurella pestis isolated from a fatal human case of plague","volume":"23","author":"Winter","year":"1960","journal-title":"Bull World Health Organ"},{"issue":"Suppl. 2","key":"10.1016\/j.micpath.2010.01.005_bib9","doi-asserted-by":"crossref","first-page":"S178","DOI":"10.1093\/clinids\/21.Supplement_2.S178","article-title":"Relationship between virulence and immunity as revealed in recent studies of the F1 capsule of Yersinia pestis","volume":"21","author":"Friedlander","year":"1995","journal-title":"Clin Infect Dis"},{"issue":"Pt 7","key":"10.1016\/j.micpath.2010.01.005_bib10","doi-asserted-by":"crossref","first-page":"1847","DOI":"10.1099\/13500872-142-7-1847","article-title":"An aroA mutant of Yersinia pestis is attenuated in guinea-pigs, but virulent in mice","volume":"142","author":"Oyston","year":"1996","journal-title":"Microbiology"},{"key":"10.1016\/j.micpath.2010.01.005_bib11","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/291238a0","article-title":"Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines","volume":"291","author":"Hoiseth","year":"1981","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2010.01.005_bib12","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/0882-4010(90)90030-T","article-title":"Construction of an auxotrophic Shigella flexneri strain for use as a live vaccine","volume":"8","author":"Lindberg","year":"1990","journal-title":"Microb Pathog"},{"key":"10.1016\/j.micpath.2010.01.005_bib13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0882-4010(87)90071-4","article-title":"Effect of different purine auxotrophic mutations on mouse-virulence of a Vi-positive strain of Salmonella dublin and of two strains of Salmonella typhimurium","volume":"3","author":"McFarland","year":"1987","journal-title":"Microb Pathog"},{"key":"10.1016\/j.micpath.2010.01.005_bib14","doi-asserted-by":"crossref","first-page":"3055","DOI":"10.1128\/IAI.64.8.3055-3061.1996","article-title":"Engineered Delta guaB-A Delta virG Shigella flexneri 2a strain CVD 1205: construction, safety, immunogenicity, and potential efficacy as a mucosal vaccine","volume":"64","author":"Noriega","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2010.01.005_bib15","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1086\/424680","article-title":"Deletion in the Shigella enterotoxin genes further attenuates Shigella flexneri 2a bearing guanine auxotrophy in a phase 1 trial of CVD 1204 and CVD 1208","volume":"190","author":"Kotloff","year":"2004","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micpath.2010.01.005_bib16","doi-asserted-by":"crossref","first-page":"268","DOI":"10.4161\/hv.4746","article-title":"Safety and Immunogenicity of CVD 1208S, a live, oral \u0394guaBA \u0394sen \u0394set Shigella flexneri 2a vaccine grown on animal-free media","volume":"3","author":"Kotloff","year":"2007","journal-title":"Hum Vaccin"},{"key":"10.1016\/j.micpath.2010.01.005_bib17","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1016\/j.vaccine.2009.02.073","article-title":"Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes","volume":"27","author":"Santiago","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2010.01.005_bib18","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1038\/35097083","article-title":"Genome sequence of Yersinia pestis, the causative agent of plague","volume":"413","author":"Parkhill","year":"2001","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2010.01.005_bib19","first-page":"59","article-title":"Virulence of Pasteurella pestis and immunity to plague","volume":"37","author":"Burrows","year":"1963","journal-title":"Ergeb\u00a0Mikrobiol Immunitatsforsch Exp Ther"},{"key":"10.1016\/j.micpath.2010.01.005_bib20","first-page":"278","article-title":"The effects of loss of different virulence determinants on the virulence and immunogenicity of strains of Pasteurella pestis","volume":"39","author":"Burrows","year":"1958","journal-title":"Br J Exp Pathol"},{"key":"10.1016\/j.micpath.2010.01.005_bib21","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1128\/IAI.72.2.908-915.2004","article-title":"Generation of Yersinia pestis attenuated strains by signature-tagged mutagenesis in search of novel vaccine candidates","volume":"72","author":"Flashner","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2010.01.005_bib22","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1128\/IAI.45.3.649-654.1984","article-title":"Growth in mouse peritoneal macrophages of Yersinia pestis lacking established virulence determinants","volume":"45","author":"Straley","year":"1984","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2010.01.005_bib23","doi-asserted-by":"crossref","first-page":"6298","DOI":"10.1128\/JB.186.18.6298-6305.2004","article-title":"Temporal global changes in gene expression during temperature transition in Yersinia pestis","volume":"186","author":"Motin","year":"2004","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2010.01.005_bib24","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1111\/j.1600-065X.2008.00674.x","article-title":"Immune defense against pneumonic plague","volume":"225","author":"Smiley","year":"2008","journal-title":"Immunol Rev"},{"key":"10.1016\/j.micpath.2010.01.005_bib25","first-page":"653","article-title":"Effectiveness of live or killed plague vaccines in man","volume":"42","author":"Meyer","year":"1970","journal-title":"Bull World Health Organ"},{"key":"10.1016\/j.micpath.2010.01.005_bib26","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1128\/CVI.00137-07","article-title":"Yersinia pestis CO92 delta yopH is a potent live, attenuated plague vaccine","volume":"14","author":"Bubeck","year":"2007","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.micpath.2010.01.005_bib27","doi-asserted-by":"crossref","first-page":"4241","DOI":"10.1128\/AEM.00940-08","article-title":"Highly efficient method for introducing successive multiple scarless gene deletions and markerless gene insertions into the Yersinia pestis chromosome","volume":"74","author":"Sun","year":"2008","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.micpath.2010.01.005_bib28","series-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"1989"},{"key":"10.1016\/j.micpath.2010.01.005_bib29","doi-asserted-by":"crossref","first-page":"6127","DOI":"10.1093\/nar\/16.13.6127","article-title":"High efficiency transformation of E. coli by high voltage electroporation","volume":"16","author":"Dower","year":"1988","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.micpath.2010.01.005_bib30","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1128\/jb.178.5.1310-1319.1996","article-title":"Flagellin A is essential for the virulence of Vibrio anguillarum","volume":"178","author":"Milton","year":"1996","journal-title":"J Bacteriol"},{"key":"10.1016\/j.micpath.2010.01.005_bib31","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1128\/IAI.64.6.2088-2094.1996","article-title":"Construction and characterization of a Yersinia enterocolitica O:8 high-temperature requirement (htrA) isogenic mutant","volume":"64","author":"Li","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2010.01.005_bib32","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1126\/science.276.5310.250","article-title":"Regulation of lipid A modifications by Salmonella typhimurium virulence genes phoP-phoQ","volume":"276","author":"Guo","year":"1997","journal-title":"Science"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401010000161?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401010000161?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:47:22Z","timestamp":1590540442000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401010000161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["S0882401010000161"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2010.01.005","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2010,5]]}}}