{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T03:53:35Z","timestamp":1783569215745,"version":"3.55.0"},"reference-count":47,"publisher":"Elsevier BV","content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2013,12]]},"DOI":"10.1016\/j.micpath.2013.09.007","type":"journal-article","created":{"date-parts":[[2013,10,9]],"date-time":"2013-10-09T22:50:16Z","timestamp":1381359016000},"page":"48-56","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":19,"special_numbering":"C","title":["Structural analysis and cross-protective efficacy of recombinant 87\u00a0kDa outer membrane protein (Omp87) of Pasteurella multocida serogroup B:2"],"prefix":"10.1016","volume":"65","author":[{"given":"Abhinendra","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Revanaiah","family":"Yogisharadhya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthannan A.","family":"Ramakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.N.","family":"Viswas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathish B.","family":"Shivachandra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2013.09.007_bib1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0378-1135(99)00183-2","article-title":"The molecular biology of Pasteurella multocida","volume":"72","author":"Hunt","year":"2000","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2006.00442.x","article-title":"Pasteurella multocida pathogenesis: 125 years after Pasteur","volume":"265","author":"Harper","year":"2006","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.micpath.2013.09.007_bib3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1017\/S146625231100003X","article-title":"A\u00a0review of haemorrhagic septicaemia in cattle and buffalo","volume":"12","author":"Shivachandra","year":"2011","journal-title":"Anim Health Res Rev"},{"key":"10.1016\/j.micpath.2013.09.007_bib4","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/S0264-410X(98)80118-7","article-title":"Haemorrhagic septicaemia vaccines","volume":"16","author":"Verma","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2013.09.007_bib5","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1136\/vr.124.19.508","article-title":"Prevention of haemorrhagic septicaemia in cattle and buffaloes with a live vaccine","volume":"124","author":"Myint","year":"1989","journal-title":"Vet Rec"},{"key":"10.1016\/j.micpath.2013.09.007_bib6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0168-1656(99)00111-X","article-title":"Candidate vaccine antigens and genes in Pasteurella multocida","volume":"73","author":"Adler","year":"1999","journal-title":"J\u00a0Biotechol"},{"key":"10.1016\/j.micpath.2013.09.007_bib7","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1016\/j.rvsc.2012.02.010","article-title":"Expression and purification of recombinant type IV fimbrial subunit protein of Pasteurella multocida serogroup B:2 in Escherichia coli","volume":"93","author":"Shivachandra","year":"2012","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.micpath.2013.09.007_bib8","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s11259-005-2495-6","article-title":"Cloning and characterization of tbpA gene encoding transferrin binding protein (TbpA) from Pasteurella multocida B:2 (strain P52)","volume":"29","author":"Shivachandra","year":"2005","journal-title":"Vet Res Commun"},{"key":"10.1016\/j.micpath.2013.09.007_bib9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/biohorizons\/hzp002","article-title":"Outer membrane proteomics of Pasteurella multocida isolates to identify putative host-specificity determinants","volume":"2","author":"Wheeler","year":"2009","journal-title":"Biosci Horizons"},{"key":"10.1016\/j.micpath.2013.09.007_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2010.01.027","article-title":"Outer membrane proteins of Pasteurella multocida","volume":"144","author":"Hatfaludi","year":"2010","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib11","doi-asserted-by":"crossref","first-page":"e39973","DOI":"10.1371\/journal.pone.0039973","article-title":"Screening of 71 P.\u00a0multocida proteins for protective efficacy in a fowl cholera infection model and characterization of the protective antigen PlpE","volume":"7","author":"Hatfaludi","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2013.09.007_bib12","doi-asserted-by":"crossref","first-page":"3460","DOI":"10.1073\/pnas.051634598","article-title":"Complete genomic sequence of Pasteurella multocida, Pm70","volume":"98","author":"May","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.micpath.2013.09.007_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1475-2859-6-3","article-title":"Identification of novel immunogens in Pasteurella multocida","volume":"6","author":"Al-Hasani","year":"2007","journal-title":"Microb Cell Fact"},{"key":"10.1016\/j.micpath.2013.09.007_bib14","first-page":"13","article-title":"Predicting the outer membrane proteome of Pasteurella multocida based on consensus prediction enhanced by results integration and manual confirmation","volume":"27","author":"E-Komon","year":"2012","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.micpath.2013.09.007_bib15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/S0959-440X(99)80064-5","article-title":"\u03b2-barrel proteins from bacterial outer membranes: structure, function and refolding","volume":"9","author":"Buchanan","year":"1999","journal-title":"Curr Opin Struct Biol"},{"key":"10.1016\/j.micpath.2013.09.007_bib16","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.1128\/IAI.64.8.3161-3167.1996","article-title":"Cloning, sequencing, expression and protective capacity of the oma87 gene encoding the Pasteurella multocida 87 kilodalton outer membrane antigen","volume":"64","author":"Ruffolo","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2013.09.007_bib17","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1053\/rvsc.2001.0470","article-title":"Cloning of 87 kDa outer membrane protein gene of Pasteurella multocida P52","volume":"70","author":"Chaudhuri","year":"2001","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.micpath.2013.09.007_bib18","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1023\/A:1020212430041","article-title":"Comparative analysis of the outer membrane protein profiles of isolates of the Pasteurella multocida (B:2) associated with haemorrhagic septicaemia","volume":"26","author":"Tomer","year":"2002","journal-title":"Vet Res Commun"},{"key":"10.1016\/j.micpath.2013.09.007_bib19","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S0378-1135(99)00190-X","article-title":"Over-expression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida","volume":"72","author":"Mitchison","year":"2000","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib20","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0378-1119(95)00049-C","article-title":"The sequencing of the 80-kDa D15 protective surface antigen of Haemophilus influenzae","volume":"156","author":"Flack","year":"1995","journal-title":"Gene"},{"key":"10.1016\/j.micpath.2013.09.007_bib21","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1016\/j.str.2008.09.014","article-title":"Crystal structure of YaeT: conformational flexibility and substrate recognition","volume":"16","author":"Gatzeva-Topalova","year":"2008","journal-title":"Structure"},{"key":"10.1016\/j.micpath.2013.09.007_bib22","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1126\/science.1143860","article-title":"Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily","volume":"317","author":"Clantin","year":"2007","journal-title":"Science"},{"key":"10.1016\/j.micpath.2013.09.007_bib23","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1093\/nar\/gkg520","article-title":"SWISS-MODEL: an automated protein homology-modeling server","volume":"31","author":"Schwede","year":"2003","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.micpath.2013.09.007_bib24","first-page":"493","article-title":"A\u00a0simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am J Hygiene"},{"key":"10.1016\/j.micpath.2013.09.007_bib25","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1128\/JCM.36.4.1096-1100.1998","article-title":"Development of PCR assays for species and type specific identification of Pasteurella multocida isolates","volume":"36","author":"Townsend","year":"1998","journal-title":"J\u00a0Clin Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib26","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.rvsc.2003.10.005","article-title":"Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay","volume":"76","author":"Gautam","year":"2004","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.micpath.2013.09.007_bib27","doi-asserted-by":"crossref","first-page":"4345","DOI":"10.1016\/j.vaccine.2008.06.029","article-title":"Vaccination with Pasteurella multocida recombinant OmpA induces strong but non-protective and deleterious Th2-type immune response in mice","volume":"12","author":"Dabo","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2013.09.007_bib28","doi-asserted-by":"crossref","DOI":"10.1007\/s11259-013-9569-7","article-title":"Carboxyl terminus heterogeneity of type IV fimbrial subunit protein of Pasteurella multocida isolates","author":"Shivachandra","year":"2013","journal-title":"Vet Res Commun"},{"key":"10.1016\/j.micpath.2013.09.007_bib29","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1006\/mpat.1998.0206","article-title":"Omp85 proteins of Neisseria gonorrhoeae and Neisseria meningitidis are similar to Haemophilus influenzae D-15-Ag and Pasteurella multocida Oma87","volume":"25","author":"Manning","year":"1998","journal-title":"Microb Pathog"},{"key":"10.1016\/j.micpath.2013.09.007_bib30","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1083\/jcb.200310092","article-title":"The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria","volume":"164","author":"Gentle","year":"2004","journal-title":"J\u00a0Cell Biol"},{"key":"10.1016\/j.micpath.2013.09.007_bib31","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.resmic.2003.11.007","article-title":"Omp85, an evolutionarily conserved bacterial protein involved in outer-membrane-protein assembly","volume":"155","author":"Voulhoux","year":"2004","journal-title":"Res Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib32","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1111\/j.1365-2958.2005.04906.x","article-title":"Molecular architecture and function of the Omp85 family of proteins","volume":"58","author":"Gentle","year":"2005","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.micpath.2013.09.007_bib33","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.tibs.2003.08.003","article-title":"a conserved domain in the FtsQ family and a class of beta-barrel outer membrane proteins","volume":"28","author":"Sanchez-Pulido","year":"2003","journal-title":"Trends Biochem Sci"},{"key":"10.1016\/j.micpath.2013.09.007_bib34","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1126\/science.1078973","article-title":"Role of a highly conserved bacterial protein in outer membrane protein assembly","volume":"299","author":"Voulhoux","year":"2003","journal-title":"Science"},{"key":"10.1016\/j.micpath.2013.09.007_bib35","doi-asserted-by":"crossref","first-page":"28281","DOI":"10.1074\/jbc.M503035200","article-title":"The evolutionary related betabarrel polypeptide transporters from P.\u00a0sativum and nostoc PCC7120 contain two distinct functional domains","volume":"280","author":"Ertel","year":"2005","journal-title":"J\u00a0Biol Chem"},{"key":"10.1016\/j.micpath.2013.09.007_bib36","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/S0959-440X(00)00120-2","article-title":"Beta-barrel membrane proteins","volume":"10","author":"Schulz","year":"2000","journal-title":"Curr Opin Struct Biol"},{"key":"10.1016\/j.micpath.2013.09.007_bib37","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.bbamcr.2004.03.008","article-title":"Protein secretion through autotransporter and two-partner pathways","volume":"1694","author":"Jacob-Dubuisson","year":"2004","journal-title":"Biochem Biophy Acta"},{"key":"10.1016\/j.micpath.2013.09.007_bib38","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S0968-0004(00)01684-4","article-title":"The alpha and the beta: protein translocation across mitochondrial and plastid outer membranes","volume":"26","author":"Gabriel","year":"2001","journal-title":"Trends Biochem Sci"},{"key":"10.1016\/j.micpath.2013.09.007_bib39","doi-asserted-by":"crossref","first-page":"4755","DOI":"10.1111\/j.1742-4658.2010.07881.x","article-title":"Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB\/Omp85 superfamily","volume":"277","author":"Delattre","year":"2010","journal-title":"FEBS J"},{"key":"10.1016\/j.micpath.2013.09.007_bib40","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0022-2836(91)90880-F","article-title":"Carboxy-terminal phenylalanine is essential for the correct assembly of a bacterial outer membrane protein","volume":"218","author":"Struyve","year":"1991","journal-title":"J\u00a0Mol Biol"},{"key":"10.1016\/j.micpath.2013.09.007_bib41","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/0076-6879(90)85008-C","article-title":"Use of T7 RNA polymerase to direct expression of cloned genes","volume":"185","author":"Studier","year":"1990","journal-title":"Meth Enzymol"},{"key":"10.1016\/j.micpath.2013.09.007_bib42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0014-5793(98)00825-4","article-title":"Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography","volume":"432","author":"Rogl","year":"1998","journal-title":"FEBS Lett"},{"key":"10.1016\/j.micpath.2013.09.007_bib43","doi-asserted-by":"crossref","first-page":"3701","DOI":"10.1128\/IAI.65.9.3701-3707.1997","article-title":"Outer membrane protein D15 is conserved among Haemophilus influenzae species and may represent a universal protective antigen against invasive disease","volume":"65","author":"Loosmore","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2013.09.007_bib44","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1086\/315399","article-title":"Opsonic potential, protective capacity, and sequence conservation of the Treponema pallidum subspecies pallidum Tp92","volume":"181","author":"Cameron","year":"2000","journal-title":"J\u00a0Infect Dis"},{"key":"10.1016\/j.micpath.2013.09.007_bib45","doi-asserted-by":"crossref","first-page":"4438","DOI":"10.1128\/IAI.69.7.4438-4446.2001","article-title":"Cloning, overexpression, purification and immunobiology of an 85-kilodalton outer membrane protein from Haemophilus ducreyi","volume":"69","author":"Thomas","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2013.09.007_bib46","doi-asserted-by":"crossref","first-page":"3349","DOI":"10.1128\/IAI.66.7.3349-3354.1998","article-title":"A\u00a020-kilodalton N-terminal fragment of the D15 protein contains a protective epitope(s) against Haemophilus infuenzae type a and type b","volume":"66","author":"Yang","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.micpath.2013.09.007_bib47","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1023\/B:VERC.0000045959.36513.e9","article-title":"Prevalent serotypes of Pasteurella multocida isolated from different animal and avian species in India","volume":"28","author":"Kumar","year":"2004","journal-title":"Vet Res Commun"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401013001344?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401013001344?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T12:30:50Z","timestamp":1596630650000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401013001344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":47,"alternative-id":["S0882401013001344"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2013.09.007","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural analysis and cross-protective efficacy of recombinant 87\u00a0kDa outer membrane protein (Omp87) of Pasteurella multocida serogroup B:2","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2013.09.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}