{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T09:12:35Z","timestamp":1782810755585,"version":"3.54.5"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2019,4]]},"DOI":"10.1016\/j.micpath.2019.02.017","type":"journal-article","created":{"date-parts":[[2019,2,13]],"date-time":"2019-02-13T03:28:24Z","timestamp":1550028504000},"page":"213-223","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"special_numbering":"C","title":["Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining"],"prefix":"10.1016","volume":"129","author":[{"given":"Elawad A.","family":"Hussein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hair-Bejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul R.","family":"Omar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siti S.","family":"Arshad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4590-8704","authenticated-orcid":false,"given":"Homayoun","family":"Hani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan N.","family":"Balakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Yakubu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Ibrahim","family":"Saeed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ideris","family":"Aini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2019.02.017_bib1","series-title":"Controlling Newcastle Disease in Village Chickens: a Field Manual, Version 14.03.2001","first-page":"112","author":"Alders","year":"2001"},{"key":"10.1016\/j.micpath.2019.02.017_bib2","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1354\/vp.45-6-928","article-title":"Pathologic and immunohistochemical studies of Newcastle disease (ND) in Broiler chickens vaccinated with ND: severe nonpurulent encephalitis and necrotizing pancreatitis","volume":"45","author":"Nakamura","year":"2008","journal-title":"J. Vet. Pathol."},{"key":"10.1016\/j.micpath.2019.02.017_bib3","series-title":"A Laboratory Manual for the Isolation and Identification of Avian Pathogens","first-page":"156","article-title":"Newcastle disease and other avian paramyxoviruses","author":"Alexander","year":"1998"},{"key":"10.1016\/j.micpath.2019.02.017_bib4","series-title":"Veterinary Virology","first-page":"411","author":"Murphy","year":"1999"},{"key":"10.1016\/j.micpath.2019.02.017_bib5","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1099\/vir.0.18714-0","article-title":"Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: nonvirulent cleavage site mutants revert to virulent to virulence after one passage in chicken brain","volume":"84","author":"de Leeuw","year":"2003","journal-title":"Gen. Virol."},{"issue":"2","key":"10.1016\/j.micpath.2019.02.017_bib6","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1354\/vp.36-2-125","article-title":"Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence","volume":"36","author":"Brown","year":"1999","journal-title":"J. Vet. Pathol."},{"key":"10.1016\/j.micpath.2019.02.017_bib7","doi-asserted-by":"crossref","first-page":"4965","DOI":"10.1128\/JVI.78.10.4965-4975.2004","article-title":"Loss of N-linked glycosylation from the hemagglutinin-neuraminidase alters virulence of Newcastle disease virus","volume":"78","author":"Panda","year":"2004","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2019.02.017_bib8","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jviromet.2009.05.006","article-title":"Detection and differentiation of velogenic and lentogenic Newcastle disease viruses using SYBR Green I real-time PCR with nucleocapsid gene-specific primers","volume":"160","author":"Tan","year":"2009","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.micpath.2019.02.017_bib9","doi-asserted-by":"crossref","first-page":"8676","DOI":"10.1128\/JVI.77.16.8676-8685.2003","article-title":"Newcastle disease virus V Protein is associated with viral pathogenesis and functions as an alpha intrferon antagonist","volume":"77","author":"Huang","year":"2003","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2019.02.017_bib10","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1637\/0005-2086(2003)047[0319:POCNDV]2.0.CO;2","article-title":"Pathogenesis of chicken-passaged Newcastle disease viruses isolated from chickens and wild and exotic birds","volume":"47","author":"Kommerss","year":"2002","journal-title":"Avian Dis"},{"key":"10.1016\/j.micpath.2019.02.017_bib11","series-title":"Color Atlas and Text of Histology","first-page":"198","author":"Gartner","year":"2014"},{"key":"10.1016\/j.micpath.2019.02.017_bib12","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1053\/he.2000.6983","article-title":"Intragraft localization of activated nuclear factor KB in recurrent hepatitis C virus disease following liver transplantation","volume":"31","author":"Gaweco","year":"2000","journal-title":"J. Hepatol."},{"issue":"2","key":"10.1016\/j.micpath.2019.02.017_bib13","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1292\/jvms.62.161","article-title":"Distribution and Developmental Change of Lymphoid Tissues in the Chicken Proventriculus","volume":"62","author":"Matsumato","year":"2000","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.micpath.2019.02.017_bib14","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1146\/annurev.immunol.021908.132629","article-title":"The liver as a lymphoid organ","volume":"27","author":"Crispe","year":"2009","journal-title":"Annual Review of Immunology"},{"key":"10.1016\/j.micpath.2019.02.017_bib15","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1161\/CIRCRESAHA.113.300890","article-title":"Monocyte and Macrophage Heterogeneity in the Heart","volume":"112","author":"Nahrendorf","year":"2013","journal-title":"Circ. Res."},{"key":"10.1016\/j.micpath.2019.02.017_bib16","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/nrneph.2014.170","article-title":"Dendritic cells and macrophages in the kidney: a spectrum of good and evil","volume":"10","author":"Rogers","year":"2014","journal-title":"Nat. Rev. Nephrol."},{"key":"10.1016\/j.micpath.2019.02.017_bib17","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1002\/dvg.20417","article-title":"Macrophages in the embryo and beyond: Much more than just giant phagocytes","volume":"46","author":"Ovchinnikov","year":"2008","journal-title":"Genesis"},{"key":"10.1016\/j.micpath.2019.02.017_bib18","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1354\/vp.43-2-168","article-title":"Pathogenesis of Newcastle disease in commercial and specific pathogen-free turkeys experimentally infected with isolates of different virulence","volume":"43","author":"Piacenti","year":"2006","journal-title":"J. Vet. Pathol."},{"key":"10.1016\/j.micpath.2019.02.017_bib19","series-title":"PCR\/RT-PCR in Situ Light and Electron Microscopy","first-page":"5","article-title":"General introduction","author":"Morel","year":"2003"},{"key":"10.1016\/j.micpath.2019.02.017_bib20","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1080\/0307945021000024580","article-title":"Early stages of infectious bursal disease virus infection in chickens detected by in situ reverse transcriptase-polymerase chain reaction","volume":"31","author":"Zhang","year":"2010","journal-title":"J. Avian Pathol."},{"key":"10.1016\/j.micpath.2019.02.017_bib21","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1186\/1743-422X-7-183","article-title":"Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia [Electronic version]","volume":"7","author":"Ayalew","year":"2010","journal-title":"Virol. J."},{"key":"10.1016\/j.micpath.2019.02.017_bib22","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1007\/s00705-003-0272-9","article-title":"Rapid pathotyping of Newcastle disease virus from allantioc fluid and organs of experimentally infected chickens using two novel probes","volume":"149","author":"Li","year":"2004","journal-title":"Arch. Virol."},{"issue":"2","key":"10.1016\/j.micpath.2019.02.017_bib23","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1080\/03079459994894","article-title":"Tropism of subgroup J avian leukosis virus as detected by in siti hybridization","volume":"28","author":"Arshad","year":"1999","journal-title":"J. Avian Pathol."},{"key":"10.1016\/j.micpath.2019.02.017_bib24","first-page":"169","article-title":"Application of reverse transcription in situ PCR in cancer analysis","volume":"vol. 334","author":"Takeuchi","year":"2006"},{"key":"10.1016\/j.micpath.2019.02.017_bib25","series-title":"PASW 18 Statistical Procedure Companion","first-page":"127","author":"Noru\u0161is","year":"2004"},{"key":"10.1016\/j.micpath.2019.02.017_bib26","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/BF01310936","article-title":"In vivo interference by Newcastle disease virus in chickens, the natural host of the virus","volume":"108","author":"Li","year":"1989","journal-title":"Arch. Virol."},{"key":"10.1016\/j.micpath.2019.02.017_bib27","series-title":"Diseases of Poultry","first-page":"452","article-title":"Newcastle disease","author":"Beard","year":"1984"}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401018312555?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401018312555?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,3,16]],"date-time":"2019-03-16T09:51:11Z","timestamp":1552729871000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401018312555"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":27,"alternative-id":["S0882401018312555"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2019.02.017","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Velogenic newcastle disease virus tissue tropism and pathogenesis of infection in chickens by application of in situ PCR, immunoperoxase staining and HE staining","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2019.02.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}