{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:57:20Z","timestamp":1790517440743,"version":"4.1.0"},"reference-count":32,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770184"],"award-info":[{"award-number":["31770184"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.micpath.2019.103568","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T21:10:54Z","timestamp":1560201054000},"page":"103568","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Determination of the cleavage site of enterovirus 71 VP0 and the effect of this cleavage on viral infectivity and assembly"],"prefix":"10.1016","volume":"134","author":[{"given":"Jiaming","family":"Cao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Hou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjun","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiheng","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlai","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2019.103568_bib1","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1093\/infdis\/169.4.905","article-title":"Enterovirus 71 infections and neurologic disease--United States, 1977-1991","volume":"169","author":"Alexander","year":"1994","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.micpath.2019.103568_bib2","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/BF01317504","article-title":"Enterovirus 71 isolated from cases of epidemic poliomyelitis-like disease in Bulgaria","volume":"60","author":"Chumakov","year":"1979","journal-title":"Arch. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib3","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1016\/S0140-6736(05)60789-1","article-title":"Neurogenic pulmonary oedema and enterovirus 71 encephalomyelitis","volume":"352","author":"Lum","year":"1998","journal-title":"Lancet"},{"key":"10.1016\/j.micpath.2019.103568_bib4","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/0022-2836(68)90195-2","article-title":"Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein","volume":"33","author":"Jacobson","year":"1968","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.micpath.2019.103568_bib5","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/jvi.12.2.291-299.1973","article-title":"Polypeptide composition of poliovirions, naturally occurring empty capsids, and 14S precursor particles","volume":"12","author":"Phillips","year":"1973","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib6","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1002\/pro.5560031005","article-title":"Role and mechanism of the maturation cleavage of VP0 in poliovirus assembly: structure of the empty capsid assembly intermediate at 2.9 A resolution","volume":"3","author":"Basavappa","year":"1994","journal-title":"Protein Sci. : Publ. Protein Soc."},{"key":"10.1016\/j.micpath.2019.103568_bib7","doi-asserted-by":"crossref","first-page":"9743","DOI":"10.1128\/jvi.71.12.9743-9752.1997","article-title":"Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus","volume":"71","author":"Curry","year":"1997","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib8","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.1128\/jvi.64.9.4067-4075.1990","article-title":"Temperature-sensitive poliovirus mutant fails to cleave VP0 and accumulates provirions","volume":"64","author":"Compton","year":"1990","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1073\/pnas.84.1.21","article-title":"Implications of the picornavirus capsid structure for polyprotein processing","volume":"84","author":"Arnold","year":"1987","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"10.1016\/j.micpath.2019.103568_bib10","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1128\/jvi.65.1.326-334.1991","article-title":"Catalysis of poliovirus VP0 maturation cleavage is not mediated by serine 10 of VP2","volume":"65","author":"Harber","year":"1991","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib11","doi-asserted-by":"crossref","first-page":"9072","DOI":"10.1128\/JVI.73.11.9072-9079.1999","article-title":"Poliovirus mutants at histidine 195 of VP2 do not cleave VP0 into VP2 and VP4","volume":"73","author":"Hindiyeh","year":"1999","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib12","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1128\/MMBR.00012-14","article-title":"Picornavirus morphogenesis","volume":"78","author":"Jiang","year":"2014","journal-title":"Microbiol. Mol. Biol. Rev. : MMBR (Microbiol. Mol. Biol. Rev.)"},{"key":"10.1016\/j.micpath.2019.103568_bib13","doi-asserted-by":"crossref","first-page":"82","DOI":"10.3390\/v8030082","article-title":"Roles of the picornaviral 3C proteinase in the viral life cycle and host cells","volume":"8","author":"Sun","year":"2016","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2019.103568_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.biocel.2006.07.006","article-title":"Foot-and-mouth disease virus 3C protease: recent structural and functional insights into an antiviral target","volume":"39","author":"Curry","year":"2007","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.micpath.2019.103568_bib15","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.virusres.2017.11.023","article-title":"Nonstructural protein 2A modulates replication and virulence of enterovirus 71","volume":"244","author":"Li","year":"2018","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2019.103568_bib16","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0168-1702(95)00087-9","article-title":"Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus","volume":"39","author":"Brown","year":"1995","journal-title":"Virus Res."},{"key":"10.1016\/j.micpath.2019.103568_bib17","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0049973","article-title":"Immunological and biochemical characterization of coxsackie virus A16 viral particles","volume":"7","author":"Chong","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103568_bib18","article-title":"Purification and characterization of enterovirus 71 viral particles produced from vero cells grown in a serum-free microcarrier bioreactor system","volume":"6","author":"Liu","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103568_bib19","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1128\/jvi.69.3.1540-1547.1995","article-title":"Amino acid substitutions in the poliovirus maturation cleavage site affect assembly and result in accumulation of provirions","volume":"69","author":"Ansardi","year":"1995","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib20","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1007\/s00705-003-0125-6","article-title":"Effects of mutations in the VP2\/VP4 cleavage site of Swine vesicular disease virus on RNA encapsidation and viral infectivity","volume":"148","author":"Rebel","year":"2003","journal-title":"Arch. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib21","doi-asserted-by":"crossref","first-page":"2778","DOI":"10.3390\/v6072778","article-title":"Identification of luteolin as enterovirus 71 and coxsackievirus A16 inhibitors through reporter viruses and cell viability-based screening","volume":"6","author":"Xu","year":"2014","journal-title":"Viruses"},{"key":"10.1016\/j.micpath.2019.103568_bib22","doi-asserted-by":"crossref","first-page":"3103","DOI":"10.1007\/s00216-014-7716-9","article-title":"Highly efficient N-glycoproteomic sample preparation by combining C(18) and graphitized carbon adsorbents","volume":"406","author":"Liu","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.micpath.2019.103568_bib23","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.aca.2016.10.044","article-title":"Specific mixing facilitates the comparative quantification of phosphorylation sites with significant dysregulations","volume":"950","author":"Liu","year":"2017","journal-title":"Anal. Chim. Acta"},{"key":"10.1016\/j.micpath.2019.103568_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003231","article-title":"Enterovirus 71 protease 2Apro targets MAVS to inhibit anti-viral type I interferon responses","volume":"9","author":"Wang","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2019.103568_bib25","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1126\/science.2994218","article-title":"Three-dimensional structure of poliovirus at 2.9 A resolution","volume":"229","author":"Hogle","year":"1985","journal-title":"Science"},{"key":"10.1016\/j.micpath.2019.103568_bib26","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1126\/science.231.4738.627.a","article-title":"Poliovirus: the molecular biology of poliovirus","volume":"231","author":"Ehrenfeld","year":"1986","journal-title":"Science"},{"key":"10.1016\/j.micpath.2019.103568_bib27","doi-asserted-by":"crossref","first-page":"8408","DOI":"10.1128\/JVI.72.10.8408-8412.1998","article-title":"Complete nucleotide sequence and genetic organization of Aichi virus, a distinct member of the Picornaviridae associated with acute gastroenteritis in humans","volume":"72","author":"Yamashita","year":"1998","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib28","doi-asserted-by":"crossref","first-page":"8847","DOI":"10.1073\/pnas.89.18.8847","article-title":"A distinct picornavirus group identified by sequence analysis","volume":"89","author":"Hyypia","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.micpath.2019.103568_bib29","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1006\/viro.1993.1636","article-title":"RNA-dependent cleavage of VP0 capsid protein in provirions of hepatitis A virus","volume":"197","author":"Bishop","year":"1993","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2019.103568_bib30","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1128\/JVI.74.7.3423-3426.2000","article-title":"Uncoating kinetics of hepatitis A virus virions and provirions","volume":"74","author":"Bishop","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib31","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1128\/jvi.67.4.2110-2122.1993","article-title":"Role of maturation cleavage in infectivity of picornaviruses: activation of an infectosome","volume":"67","author":"Lee","year":"1993","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103568_bib32","first-page":"49","article-title":"Picornavirus protein processing--enzymes, substrates, and genetic regulation","volume":"161","author":"Lawson","year":"1990","journal-title":"Curr. Top. Microbiol. Immunol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019303316?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019303316?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:22:49Z","timestamp":1760109769000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401019303316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":32,"alternative-id":["S0882401019303316"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103568","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Determination of the cleavage site of enterovirus 71 VP0 and the effect of this cleavage on viral infectivity and assembly","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103568","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"103568"}}