{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:34:38Z","timestamp":1789616078052,"version":"build-2803163510"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2019,9]]},"DOI":"10.1016\/j.micpath.2019.103574","type":"journal-article","created":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T22:26:07Z","timestamp":1559600767000},"page":"103574","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":38,"special_numbering":"C","title":["Potential efficiency of conventional and advanced approaches used to detect Mycobacterium bovis in cattle"],"prefix":"10.1016","volume":"134","author":[{"given":"Abdelazeem M.","family":"Algammal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Wahdan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud M.","family":"Elhaig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2019.103574_bib1","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1111\/tbed.12291","article-title":"Prevalence of bovine tuberculosis in Egyptian cattle and the standardization of the interferon\u2010gamma assay as an ancillary test","volume":"63","author":"Abdellrazeq","year":"2016","journal-title":"Transboundary Emerg. Dis."},{"key":"10.1016\/j.micpath.2019.103574_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006406","article-title":"Genetic profiling of Mycobacterium bovis strains from slaughtered cattle in Eritrea","volume":"12","author":"Ghebremariam","year":"2018","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103574_bib3","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.prevetmed.2009.02.006","article-title":"Risk factors of bovine tuberculosis in cattle in rural livestock production systems of Ethiopia","volume":"89","author":"Tschopp","year":"2009","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.micpath.2019.103574_bib4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1517\/14728222.6.1.21","article-title":"Emerging therapeutic targets in tuberculosis: post-genomic era","volume":"6","author":"Khasnobis","year":"2002","journal-title":"Expert Opin. Ther. Targets"},{"key":"10.1016\/j.micpath.2019.103574_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0012205","article-title":"Bovine tuberculosis at the wildlife-livestock-human interface in hamer woreda, south omo, southern Ethiopia","volume":"5","author":"Tschopp","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103574_bib6","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1128\/CDLI.7.2.155-160.2000","article-title":"Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10","volume":"7","author":"Van Pinxteren","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.micpath.2019.103574_bib7","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.vetmic.2008.05.022","article-title":"Isolation of Mycobacterium bovis and other mycobacterial species from ferrets and stoats","volume":"132","author":"de Lisle","year":"2008","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib8","doi-asserted-by":"crossref","first-page":"S44","DOI":"10.1016\/j.rvsc.2014.04.002","article-title":"Current ante-mortem techniques for diagnosis of bovine tuberculosis","volume":"97","author":"Bezos","year":"2014","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib9","first-page":"13","article-title":"Identification of Mycobacterium bovis infected cattle by immunological and molecular methods","volume":"34","author":"Otero","year":"2003","journal-title":"Vet. Mex."},{"key":"10.1016\/j.micpath.2019.103574_bib10","first-page":"180","article-title":"Diagnosis of bovine tuberculosis by PPD-ELISA and sonication-ELISA","volume":"68","author":"Sayin","year":"2013","journal-title":"Isr. J. Vet. Med."},{"key":"10.1016\/j.micpath.2019.103574_bib11","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.vetmic.2005.11.029","article-title":"Diagnosis of Mycobacterium bovis infection in cattle by use of the gamma-interferon (Bovigam\u00ae) assay","volume":"112","author":"Gormley","year":"2006","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib12","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1136\/vr.160.19.660","article-title":"Influence of skin testing and blood storage on interferon-\u03b3 production in cattle affected naturally with","volume":"160","author":"Coad","year":"2007","journal-title":"Vet. Rec."},{"key":"10.1016\/j.micpath.2019.103574_bib13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.meatsci.2015.03.017","article-title":"Evaluation of the efficiency of nested q-PCR in the detection of Mycobacterium tuberculosis complex directly from tuberculosis-suspected lesions in post-mortem macroscopic inspections of bovine carcasses slaughtered in the state of Mato Grosso, Brazil","volume":"106","author":"Carvalho","year":"2015","journal-title":"Meat Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0207614","article-title":"Second line molecular diagnosis for bovine tuberculosis to improve diagnostic schemes","volume":"13","author":"Michelet","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103574_bib15","series-title":"Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2009","author":"OIE","year":"2009"},{"key":"10.1016\/j.micpath.2019.103574_bib16","first-page":"1015","article-title":"Fractionation and immunoreactivity of membrane associated proteins of Mycobacterium bovis AN5","volume":"70","author":"Gupta","year":"2000","journal-title":"Indian J. Anim. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib17","doi-asserted-by":"crossref","first-page":"4326","DOI":"10.1128\/JCM.01184-06","article-title":"Optimizing antigen cocktails for detection of Mycobacterium bovis in herds with different prevalences of bovine tuberculosis: ESAT6-CFP10 mixture shows optimal sensitivity and specificity","volume":"44","author":"Aagaard","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib18","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.micpath.2019.103574_bib19","first-page":"198","article-title":"In vitro studies on polysaccharides of Mycobacterium tuberculosis and delayed hypersensitivity","volume":"96","author":"Heilman","year":"1967","journal-title":"Am. Rev. Respir. Dis."},{"key":"10.1016\/j.micpath.2019.103574_bib20","first-page":"72","article-title":"The BOVIGAM assay as ancillary test to the tuberculin skin test","volume":"16","author":"Vordermeier","year":"2006","journal-title":"Gov Vet J"},{"key":"10.1016\/j.micpath.2019.103574_bib21","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1128\/CVI.00150-09","article-title":"Optimization of a whole-blood gamma interferon assay for detection of Mycobacterium bovis-infected cattle","volume":"16","author":"Schiller","year":"2009","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.micpath.2019.103574_bib22","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0378-1135(94)00096-F","article-title":"Detection of Mycobacterium bovis in tissues by polymerase chain reaction","volume":"43","author":"Wards","year":"1995","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib23","doi-asserted-by":"crossref","first-page":"2724","DOI":"10.1128\/JCM.42.6.2724-2732.2004","article-title":"Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis","volume":"42","author":"Prabhakar","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib24","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.20506\/rst.25.3.1717","article-title":"Comparative prevalence of tuberculosis in two dairy herds in India","volume":"25","author":"Mukherjee","year":"2006","journal-title":"Rev. Sci. Tech. Off. Int. Epizoot."},{"key":"10.1016\/j.micpath.2019.103574_bib25","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.rvsc.2016.06.014","article-title":"Risk factors for failure to detect bovine tuberculosis in cattle from infected herds across Northern Ireland (2004\u20132010)","volume":"107","author":"Lahuerta-Marin","year":"2016","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib26","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1746-6148-10-14","article-title":"Risk factors associated with negative in-vivo diagnostic results in bovine tuberculosis-infected cattle in Spain","volume":"10","author":"\u00c1lvarez","year":"2014","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.micpath.2019.103574_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0179847","article-title":"Evaluating diagnostic tests for bovine tuberculosis in the southern part of Germany: a latent class analysis","volume":"12","author":"Pucken","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103574_bib28","article-title":"Abattoir based prevalence, economic losses and veterinarians' high-risk practices survey of bovine tuberculosis in mid-delta of Egypt","volume":"49","author":"Elmonir","year":"2016","journal-title":"Alexandria J. Vet. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0008215","article-title":"Bayesian receiver operating characteristic estimation of multiple tests for diagnosis of bovine tuberculosis in Chadian cattle","volume":"4","author":"M\u00fcller","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103574_bib30","doi-asserted-by":"crossref","first-page":"512","DOI":"10.17221\/5585-VETMED","article-title":"A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR examination of tissues with tuberculous lesions in cattle in Ethiopia","volume":"51","author":"Shitaye","year":"2006","journal-title":"Vet. Med. Praha"},{"key":"10.1016\/j.micpath.2019.103574_bib31","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.prevetmed.2009.02.003","article-title":"Comparative assessment of fluorescence polarization and tuberculin skin testing for the diagnosis of bovine tuberculosis in Chadian cattle","volume":"89","author":"Ngandolo","year":"2009","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.micpath.2019.103574_bib32","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1080\/21505594.2016.1191729","article-title":"Evidence of Mycobacterium tuberculosis complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA","volume":"7","author":"Swift","year":"2016","journal-title":"Virulence"},{"key":"10.1016\/j.micpath.2019.103574_bib33","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0378-1135(94)90046-9","article-title":"Post mortem diagnosis of Mycobacterium bovis infection in cattle","volume":"40","author":"Comer","year":"1994","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib34","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s11250-017-1232-9","article-title":"Occurrence and distribution of bovine TB pathology by age, sex, and breed of cattle slaughtered in Gusau Abattoir, Zamfara State Nigeria","volume":"49","author":"Ahmad","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.micpath.2019.103574_bib35","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s11250-014-0573-x","article-title":"Comparative assessment of postmortem inspection and immunochromatographic techniques for the detection of bovine tuberculosis in slaughter cattle in Nigeria","volume":"46","author":"Okoro","year":"2014","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.micpath.2019.103574_bib36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002417","article-title":"First-time detection of Mycobacterium bovis in livestock tissues and milk in the West Bank, Palestinian Territories","volume":"7","author":"Ereqat","year":"2013","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103574_bib37","doi-asserted-by":"crossref","first-page":"463","DOI":"10.4314\/ovj.v8i4.18","article-title":"Survey of bovine tuberculosis in Nigerian beef cattle","volume":"8","author":"Ahmad","year":"2018","journal-title":"Open Vet. J."},{"key":"10.1016\/j.micpath.2019.103574_bib38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.4142\/jvs.2018.19.1.45","article-title":"Investigation of bovine tuberculosis outbreaks by using a trace-back system and molecular typing in Korean Hanwoo beef cattle","volume":"19","author":"Ku","year":"2018","journal-title":"J. Vet. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib39","doi-asserted-by":"crossref","first-page":"862","DOI":"10.14202\/vetworld.2016.862-868","article-title":"Evaluation of complementary diagnostic tools for bovine tuberculosis detection in dairy herds from India","volume":"9","author":"Thakur","year":"2016","journal-title":"Vet. World"},{"key":"10.1016\/j.micpath.2019.103574_bib40","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1292\/jvms.18-0364","article-title":"ELISA using a recombinant chimera of ESAT-6\/MPB70\/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle","volume":"81","author":"SOUZA","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.micpath.2019.103574_bib41","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.vetmic.2011.02.026","article-title":"Determination of decisional cut-off values for the optimal diagnosis of bovine tuberculosis with a modified IFN\u03b3 assay (Bovigam\u00ae) in a low prevalence area in France","volume":"151","author":"Faye","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103574_bib42","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1128\/CVI.00519-13","article-title":"Clinical and diagnostic developments of a gamma interferon release assay for use in bovine tuberculosis control programs","volume":"20","author":"Bass","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.micpath.2019.103574_bib43","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1128\/CVI.05668-11","article-title":"Specificity of the tuberculin skin test is modified by use of a protein cocktail containing ESAT-6 and CFP-10 in cattle naturally infected with Mycobacterium bovis","volume":"19","author":"Flores-Villalva","year":"2012","journal-title":"Clin. Vaccine Immunol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019305832?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019305832?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:22:01Z","timestamp":1760109721000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401019305832"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9]]},"references-count":43,"alternative-id":["S0882401019305832"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103574","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Potential efficiency of conventional and advanced approaches used to detect Mycobacterium bovis in cattle","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103574","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103574"}}