{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T07:13:40Z","timestamp":1785827620442,"version":"3.56.0"},"reference-count":78,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.micpath.2019.103704","type":"journal-article","created":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T18:05:13Z","timestamp":1567274713000},"page":"103704","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"special_numbering":"C","title":["Genome based screening of epitope ensemble vaccine candidates against dreadful visceral leishmaniasis using immunoinformatics approach"],"prefix":"10.1016","volume":"136","author":[{"given":"Garima","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Pritam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monisha","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhilesh Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satarudra Prakash","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2019.103704_bib1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003053","article-title":"Determinants for the development of visceral leishmaniasis disease","volume":"9","author":"McCall","year":"2013","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.micpath.2019.103704_bib2","author":"WHO"},{"key":"10.1016\/j.micpath.2019.103704_bib3","doi-asserted-by":"crossref","first-page":"750","DOI":"10.4269\/ajtmh.13-0096","article-title":"In vitro susceptibility of Leishmania donovani to miltefosine in Indian visceral leishmaniasis","volume":"89","author":"Prajapati","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.micpath.2019.103704_bib4","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.meegid.2008.02.011","article-title":"An in silico immunological approach for prediction of CD8+ T-cell epitopes of Leishmania major proteins in susceptible BALB\/c and resistant C57BL\/6 murine models of infection","volume":"9","author":"Guerfali","year":"2009","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.micpath.2019.103704_bib5","doi-asserted-by":"crossref","first-page":"112","DOI":"10.3389\/fcimb.2018.00112","article-title":"Analysis of the antigenic and prophylactic properties of the Leishmania translation initiation factors eIF2 and eIF2B in natural and experimental leishmaniasis","volume":"8","author":"Garde","year":"2018","journal-title":"FronT-cell Infect Microbiol."},{"key":"10.1016\/j.micpath.2019.103704_bib6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0003091","article-title":"Combining cationic liposomal delivery with MPL-TDM for cysteine protease cocktail vaccination against Leishmania donovani: evidence for antigen synergy and protection","volume":"8","author":"Das","year":"2014","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0005820","article-title":"Identification of novel Leishmania donovani antigens that help define correlates of vaccine-mediated protection in visceral leishmaniasis","volume":"4","author":"Bhowmick","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103704_bib8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.vaccine.2009.09.114","article-title":"Recombinant Leishmania tarentolae expressing the A2 virulence gene as a novel candidate vaccine against visceral leishmaniasis","volume":"28","author":"Mizbani","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2019.103704_bib9","doi-asserted-by":"crossref","first-page":"2992","DOI":"10.1016\/j.vaccine.2015.12.071","article-title":"Status of vaccine research and development of vaccines for leishmaniasis","volume":"34","author":"Gillespie","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2019.103704_bib10","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.compbiolchem.2017.12.008","article-title":"Proteome-scale identification of Leishmania infantum for novel vaccine candidates: a hierarchical subtractive approach","volume":"72","author":"Vakili","year":"2018","journal-title":"Comput. Biol. Chem."},{"key":"10.1016\/j.micpath.2019.103704_bib11","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1186\/gm113","article-title":"Clues into pathogen infection and clinicalapplications","volume":"1","author":"Ranganathan","year":"2009","journal-title":"Genome Med."},{"key":"10.1016\/j.micpath.2019.103704_bib12","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/nyas.12046","article-title":"Reverse vaccinology in the 21st century: improvements over the original design","volume":"1285","author":"Donati","year":"2013","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.micpath.2019.103704_bib13","doi-asserted-by":"crossref","first-page":"12385","DOI":"10.1038\/srep12385","article-title":"Erratum: genome analysis of excretory\/secretory proteins in taenia solium reveals their abundance of antigenic regions (AAR)","volume":"5","author":"Gomez","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2019.103704_bib14","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.meegid.2017.12.008","article-title":"Structural vaccinology considerations for in silico designing of a multi-epitope vaccine","volume":"58","author":"Negahdaripour","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.micpath.2019.103704_bib15","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3389\/fmicb.2017.00128","article-title":"Secretome prediction of two M. tuberculosis clinical isolates reveals their high antigenic density and potential drug targets","volume":"8","author":"Cornejo-Granados","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2019.103704_bib16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.biologicals.2014.11.001","article-title":"Production of a novel multi-epitope peptide vaccine for cancer immunotherapy in TC-1 tumor-bearing mice","volume":"43","author":"Nezafat","year":"2015","journal-title":"Biologicls"},{"key":"10.1016\/j.micpath.2019.103704_bib17","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1007\/s10989-015-9489-1","article-title":"In silico sub-unit hexavalent vaccine against Staphylococcus aureus biofilm-related infection","volume":"1","author":"Shahbazi","year":"2016","journal-title":"Int. J. Pept. Res. Ther."},{"key":"10.1016\/j.micpath.2019.103704_bib18","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12920-018-0354-x","article-title":"HLA and proteasome expression body map","volume":"11","author":"Boegel","year":"2018","journal-title":"BMC Med. Genomics"},{"key":"10.1016\/j.micpath.2019.103704_bib19","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1128\/IAI.72.2.863-870.2004","article-title":"Characterization of immunoglobulin G and its subclass response to Indian kala-azar infection before and after chemotherapy","volume":"72","author":"Ravindran","year":"2004","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2019.103704_bib20","doi-asserted-by":"crossref","first-page":"e35","DOI":"10.1038\/cti.2015.6","article-title":"From mouse to man: safety, immunogenicity and efficacy of a candidate leish-maniasis vaccine LEISH-F3 + GLA-SE","volume":"4","author":"Coler","year":"2015","journal-title":"Clin Transl Immunol"},{"key":"10.1016\/j.micpath.2019.103704_bib21","doi-asserted-by":"crossref","first-page":"8285","DOI":"10.1038\/s41598-017-08842-w","article-title":"Exploring Leishmania secretory proteins to design B and T-cell multi-epitope subunit vaccine using immunoinformatics approach","volume":"7","author":"Khatoon","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2019.103704_bib22","first-page":"30212","article-title":"Impact of epitope density on CD8+ T-cell development and function","volume":"5890","author":"Cosma","year":"2019","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib23","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/CVI.00208-09","article-title":"Peptide microarray-based identification of Mycobacterium tuberculosis epitope binding to HLA-DRB1*0101, DRB1*1501, and DRB1*0401","volume":"17","author":"Gaseitsiwe","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005311","article-title":"Identification of BALB\/c immune markers correlated with a partial protection to Leishmania infantum after vaccination with a rationally designed multi-epitope cysteine protease a peptide-based nanovaccine","volume":"11","author":"Agallou","year":"2017","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0166-6851(95)00035-Y","article-title":"Isolation and characterization of the gene encoding the surface membrane 3'-nucleotidase\/nuclease of Leishmania donovani","volume":"71","author":"Debrabant","year":"1995","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.micpath.2019.103704_bib26","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.molimm.2017.11.024","article-title":"In silico prediction of potential vaccine candidates on capsid rotein of human bocavirus 1","volume":"93","author":"Kalyanaraman","year":"2018","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib27","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s12859-017-1577-0","article-title":"The prediction of a pathogenesis-related secretome of Puccinia helianthi through high-throughput transcriptome analysis","volume":"18","author":"Jing","year":"2017","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.micpath.2019.103704_bib28","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A combined transmembrane topology and signal peptide prediction method","volume":"338","author":"K\u00e4ll","year":"2004","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.micpath.2019.103704_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005527","article-title":"A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis: first-in-human trial of Chad63-KH","volume":"11","author":"Osman","year":"2017","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib30","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","article-title":"High-throughput prediction of protein antigenicity using protein microarray data","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2019.103704_bib31","doi-asserted-by":"crossref","first-page":"234ra56","DOI":"10.1126\/scitranslmed.3008222","article-title":"Modular multiantigen T-cell epitope-enriched DNA vaccine against human leishmaniasis","volume":"6","author":"Das","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.micpath.2019.103704_bib32","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.humimm.2009.11.009","article-title":"Computational characterization of Plasmodium falciparum proteomic data for screening of potential vaccine candidates","volume":"71","author":"Singh","year":"2010","journal-title":"Hum. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib33","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1186\/s12859-018-2482-x","article-title":"Exploitation of reverse vaccinology and immunoinformatics as promising platform for genome-wide screening of new effective vaccine candidates against Plasmodium falciparum","volume":"19","author":"Pritam","year":"2019","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.micpath.2019.103704_bib34","first-page":"763461","article-title":"Automatic generation of validated specific epitope sets","author":"Carrasco Pro","year":"2015","journal-title":"J Immunol Res"},{"key":"10.1016\/j.micpath.2019.103704_bib35","first-page":"709216","article-title":"Genome-wide prediction of vaccine candidates for Leishmania major: an integrated approach","author":"Singh","year":"2015","journal-title":"J. Trop. Med."},{"key":"10.1016\/j.micpath.2019.103704_bib36","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1186\/s12879-016-1626-8","article-title":"Association of HLA class I and II genes with cutaneous leishmaniasis: a case control study from Sri Lanka and a systematic review","volume":"16","author":"Samaranayake","year":"2016","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib37","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nprot.2016.169","article-title":"The ClusPro web server for protein-protein docking","volume":"12","author":"Kozakov","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.micpath.2019.103704_bib38","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1038\/s41598-018-22592-3","article-title":"Proteome-wide mapping of immune features onto Plasmodium protein three-dimensional structures","volume":"8","author":"Guy","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2019.103704_bib39","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005791","article-title":"Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites","volume":"11","author":"Swearingen","year":"2017","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib40","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1074\/mcp.M600035-MCP200","article-title":"Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum","volume":"5","author":"Gilson","year":"2006","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.micpath.2019.103704_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.6026\/97320630004001","article-title":"Identification and characterization of merozoite surface protein 1 epitope","volume":"4","author":"Singh","year":"2009","journal-title":"Bioinformation"},{"key":"10.1016\/j.micpath.2019.103704_bib42","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1093\/occmed\/kqm110","article-title":"Active and passive immunity, vaccine types, excipients and licensing","volume":"57","author":"Baxter","year":"2007","journal-title":"Occup. Med. (Lond.)"},{"key":"10.1016\/j.micpath.2019.103704_bib43","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1586\/14760584.2014.922417","article-title":"Development of live attenuated influenza vaccines against pandemic influenza strains","volume":"13","author":"Coelingh","year":"2014","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.micpath.2019.103704_bib44","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.chom.2015.02.012","article-title":"Influenza vaccines: challenges and solutions","volume":"17","author":"Houser","year":"2015","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.micpath.2019.103704_bib45","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1093\/infdis\/jir842","article-title":"Therapeutic vaccination with recombinant adenovirus reduces splenic parasite burden in experimental visceral leishmaniasis","volume":"205","author":"Maroof","year":"2012","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib46","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009862","article-title":"Computational immunology meets bioinformatics: the use of prediction tools for molecular binding in the simulation of the immune system","volume":"5","author":"Rapin","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103704_bib47","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3389\/fimmu.2012.00251","article-title":"Immunobiology of visceral leishmaniasis","volume":"3","author":"Kumar","year":"2012","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib48","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/B978-0-12-407679-2.00005-3","article-title":"Mechanisms of immune evasion in leishmaniasis","volume":"82","author":"Gupta","year":"2013","journal-title":"Adv. Appl. Microbiol."},{"key":"10.1016\/j.micpath.2019.103704_bib49","doi-asserted-by":"crossref","first-page":"4306","DOI":"10.4049\/jimmunol.145.12.4306","article-title":"Tumor necrosis factor-alpha synergizes with IFN-gamma in mediating killing of Leishmania major through the induction of nitric oxide","volume":"145","author":"Liew","year":"1990","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib50","doi-asserted-by":"crossref","first-page":"S4","DOI":"10.1186\/1471-2164-14-S6-S4","article-title":"Mature Epitope Density--a strategy for target selection based on immunoinformatics and exported prokaryotic proteins","volume":"14","author":"Santos","year":"2013","journal-title":"BMC Genomics"},{"key":"10.1016\/j.micpath.2019.103704_bib51","doi-asserted-by":"crossref","first-page":"278","DOI":"10.3389\/fimmu.2017.00278","article-title":"The immune epitope database and analysis resource in epitope discovery and synthetic vaccine design","volume":"8","author":"Fleri","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib52","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/irv.12513","article-title":"T-cell epitope content comparison (EpiCC) of swine H1 influenza A virus hemagglutinin","volume":"11","author":"Gutierrez","year":"2017","journal-title":"Influenza Other Respir Viruses"},{"key":"10.1016\/j.micpath.2019.103704_bib53","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1111\/imm.12194","article-title":"Prediction and characterization of helper T-cell epitopes from pneumococcal surface adhesin A","volume":"141","author":"Singh","year":"2014","journal-title":"Immunology"},{"key":"10.1016\/j.micpath.2019.103704_bib54","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1086\/379084","article-title":"A major susceptibility locus on chromosome 22q12 plays a critical role in the control of kala-azar","volume":"73","author":"Bucheton","year":"2003","journal-title":"Am. J. Hum. Genet."},{"key":"10.1016\/j.micpath.2019.103704_bib55","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/0198-8859(91)90088-Q","article-title":"Association of mucosal leishmaniasis with HLA","volume":"32","author":"Petzl-Erler","year":"1991","journal-title":"Hum. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib56","first-page":"175","article-title":"Allele and haplotype frequencies at human leukocyte antigen class I and II genes in Venezuela's population","volume":"70","author":"Del Pilar Fortes","year":"2012","journal-title":"Ann. Biol. Clin. (Paris)"},{"key":"10.1016\/j.micpath.2019.103704_bib57","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1399-0039.1997.tb02768.x","article-title":"HLA-DR 52- and 51-associated DRB1 alleles in Kenya, east Africa","volume":"49","author":"Dunand","year":"1997","journal-title":"Tissue Antigens"},{"key":"10.1016\/j.micpath.2019.103704_bib58","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.3389\/fimmu.2017.01763","article-title":"Identification of potential MHC class-II-restricted epitopes derived from Leishmania donovani antigens by reverse vaccinology and evaluation of their CD4+ T-cell responsiveness against visceral leishmaniasis","volume":"8","author":"Dikhit","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib59","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1111\/cea.12507","article-title":"Definition of a pool of epitopes that recapitulates the T-cell reactivity against major house dust mite allergens","volume":"45","author":"Hinz","year":"2015","journal-title":"Clin. Exp. Allergy"},{"key":"10.1016\/j.micpath.2019.103704_bib60","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1111\/imm.12984","article-title":"Development of a novel clustering tool for linear peptide sequences","volume":"155","author":"Dhanda","year":"2018","journal-title":"Immunology"},{"key":"10.1016\/j.micpath.2019.103704_bib61","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1003266","article-title":"Properties of MHC class I presented peptides that enhance immunogenicity","volume":"9","author":"Calis","year":"2013","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.micpath.2019.103704_bib62","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1084\/jem.20050864","article-title":"The immunogenicity of a viral cytotoxic T-cell epitope is controlled by its MHC-bound conformation","volume":"202","author":"Tynan","year":"2005","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.micpath.2019.103704_bib63","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/j.0966-0410.2004.00480.x","article-title":"Home chemotherapy for children with cancer: perspectives from healthcare professionals","volume":"12","author":"Stevens","year":"2004","journal-title":"Health Soc. Care Commun."},{"key":"10.1016\/j.micpath.2019.103704_bib64","doi-asserted-by":"crossref","first-page":"7064","DOI":"10.4049\/jimmunol.165.12.7064","article-title":"Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral leishmaniasis","volume":"165","author":"Stager","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib65","doi-asserted-by":"crossref","first-page":"135","DOI":"10.4103\/0974-777X.62876","article-title":"Immunological perspectives of leishmaniasis","volume":"2","author":"Nyl\u00e9n","year":"2010","journal-title":"J. Glob. Infect. Dis."},{"key":"10.1016\/j.micpath.2019.103704_bib66","doi-asserted-by":"crossref","first-page":"15053","DOI":"10.1038\/s41598-017-15354-0","article-title":"Novel Plasmodium antigens identified via genome-based antibody screen induce protection associated with polyfunctional T-cell responses","volume":"7","author":"Schussek","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2019.103704_bib67","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1111\/ijd.13709","article-title":"Significance of interferon gamma in the prediction of successful therapy of common warts by intralesional injection of Candida antigen","volume":"56","author":"Nofal","year":"2017","journal-title":"Int. J. Dermatol."},{"key":"10.1016\/j.micpath.2019.103704_bib68","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.1084\/jem.20082901","article-title":"Peptide immunotherapy in allergic asthma generates IL-10-dependent immunological tolerance associated with linked epitope suppression","volume":"206","author":"Campbell","year":"2009","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.micpath.2019.103704_bib69","doi-asserted-by":"crossref","first-page":"2710","DOI":"10.1158\/0008-5472.CAN-13-3286","article-title":"Elimination of IL-10-inducing T-helper epitopes from an IGFBP-2 vaccine ensures potent antitumor activity","volume":"74","author":"Cecil","year":"2014","journal-title":"Cancer Res."},{"key":"10.1016\/j.micpath.2019.103704_bib70","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1038\/ni.1894","article-title":"Programming dendritic cells to induce T(H)2 and tolerogenic responses","volume":"11","author":"Pulendran","year":"2010","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib71","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1186\/s12967-018-1560-1","article-title":"Computer-aided prediction of antigen presenting cell modulators for designing peptide-based vaccine adjuvants","volume":"16","author":"Nagpal","year":"2018","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.micpath.2019.103704_bib72","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.humimm.2015.11.012","article-title":"Major histocompatibility complex linked databases and prediction tools for designing vaccines","volume":"77","author":"Singh","year":"2016","journal-title":"Hum. Immunol."},{"key":"10.1016\/j.micpath.2019.103704_bib73","doi-asserted-by":"crossref","first-page":"72","DOI":"10.6026\/97320630003072","article-title":"Ranking of binding and nonbinding peptides to MHC class I molecules using inverse folding approach: implications for vaccine design","volume":"3","author":"Singh","year":"2008","journal-title":"Bioinformation"},{"key":"10.1016\/j.micpath.2019.103704_bib74","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1007\/s13205-017-0947-7","article-title":"Genome-wide identification of novel vaccine candidates for Plasmodium falciparum malaria using integrative bioinformatics approaches","volume":"7","author":"Singh","year":"2017","journal-title":"3 Biotech"},{"key":"10.1016\/j.micpath.2019.103704_bib75","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.micpath.2019.05.010","article-title":"Conserved B and T-cell epitopes prediction of ebola virus glycoprotein for vaccine development: an immuno-informatics approach","volume":"7","author":"Ahmad","year":"2019","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2019.103704_bib76","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.micpath.2019.01.007","article-title":"In silico analysis of four structural proteins of aphthovirus serotypes revealed significant B and T-cell epitopes","volume":"128","author":"Raza","year":"2019","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2019.103704_bib77","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1006457","article-title":"Systematically benchmarking peptide-MHC binding predictors: from synthetic to naturally processed epitopes","volume":"14","author":"Zhao","year":"2018","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.micpath.2019.103704_bib78","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1080\/14787210.2019.1577731","article-title":"Strategies to enhance access to diagnosis and treatment for Chagas disease patients in Latin America","volume":"17","author":"Alonso-Padilla","year":"2019","journal-title":"Expert Rev. Anti Infect. Ther."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019309222?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019309222?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:24:49Z","timestamp":1760109889000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401019309222"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":78,"alternative-id":["S0882401019309222"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103704","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genome based screening of epitope ensemble vaccine candidates against dreadful visceral leishmaniasis using immunoinformatics approach","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103704","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103704"}}