{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:23:35Z","timestamp":1788909815805,"version":"build-2803163510"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010888","name":"Wuhan Municipal Science and Technology Bureau","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010888","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.micpath.2019.103706","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T13:07:49Z","timestamp":1567516069000},"page":"103706","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer"],"prefix":"10.1016","volume":"136","author":[{"given":"Ying","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuehuan","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zutao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sishun","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaowen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuncai","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deshi","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingren","family":"Bi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zili","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2019.103706_bib1","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1099\/00207713-43-4-768","article-title":"Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group","volume":"43","author":"Segers","year":"1993","journal-title":"Int. J. Syst. Bacteriol."},{"key":"10.1016\/j.micpath.2019.103706_bib2","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1080\/03079450220136576","article-title":"A potential new serotype of Riemerella anatipestifer isolated from ducks in Thailand","volume":"31","author":"Pathanasophon","year":"2002","journal-title":"Avian Pathol."},{"key":"10.1016\/j.micpath.2019.103706_bib3","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/CDLI.7.2.168-174.2000","article-title":"Characterization of a predominant immunogenic outer membrane protein of Riemerella anatipestifer","volume":"7","author":"Subramaniam","year":"2000","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.micpath.2019.103706_bib4","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/j.1472-765X.2006.02053.x","article-title":"Development of a novel PCR assay specific for Riemerella anatipestifer","volume":"44","author":"Kardos","year":"2007","journal-title":"Lett. Appl. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/nrmicro3241","article-title":"CRISPR-Cas systems: beyond adaptive immunity","volume":"12","author":"Westra","year":"2014","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib6","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1146\/annurev-biochem-072911-172315","article-title":"CRISPR-mediated adaptive immune systems in bacteria and archaea","volume":"82","author":"Sorek","year":"2013","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.micpath.2019.103706_bib7","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1146\/annurev.micro.112408.134123","article-title":"CRISPR\/Cas system and its role in phage-bacteria interactions","volume":"64","author":"Deveau","year":"2010","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib8","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1126\/science.1138140","article-title":"CRISPR provides acquired resistance against viruses in prokaryotes","volume":"315","author":"Barrangou","year":"2007","journal-title":"Science (New York, NY)"},{"key":"10.1016\/j.micpath.2019.103706_bib9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nrg2749","article-title":"CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea","volume":"11","author":"Marraffini","year":"2010","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.micpath.2019.103706_bib10","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1042\/BST20130038","article-title":"The basic building blocks and evolution of CRISPR-CAS systems","volume":"41","author":"Makarova","year":"2013","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.micpath.2019.103706_bib11","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1046\/j.1365-2958.2002.02839.x","article-title":"Identification of genes that are associated with DNA repeats in prokaryotes","volume":"43","author":"Jansen","year":"2002","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib12","doi-asserted-by":"crossref","first-page":"e60","DOI":"10.1371\/journal.pcbi.0010060","article-title":"A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR\/Cas subtypes exist in prokaryotic genomes","volume":"1","author":"Haft","year":"2005","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.micpath.2019.103706_bib13","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1745-6150-1-7","article-title":"A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action","volume":"1","author":"Makarova","year":"2006","journal-title":"Biol. Direct"},{"key":"10.1016\/j.micpath.2019.103706_bib14","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1093\/nar\/30.2.482","article-title":"A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis","volume":"30","author":"Makarova","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2019.103706_bib15","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1038\/nrmicro3569","article-title":"An updated evolutionary classification of CRISPR-Cas systems","volume":"13","author":"Makarova","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib16","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nrmicro2577","article-title":"Evolution and classification of the CRISPR-Cas systems","volume":"9","author":"Makarova","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib17","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/nbt.2508","article-title":"RNA-guided editing of bacterial genomes using CRISPR-Cas systems","volume":"31","author":"Jiang","year":"2013","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.micpath.2019.103706_bib18","doi-asserted-by":"crossref","first-page":"1975","DOI":"10.1093\/mp\/sst119","article-title":"RNA-guided genome editing in plants using a CRISPR-Cas system","volume":"6","author":"Xie","year":"2013","journal-title":"Mol. Plant"},{"key":"10.1016\/j.micpath.2019.103706_bib19","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nprot.2016.171","article-title":"Precise and efficient scarless genome editing in stem cells using CORRECT","volume":"12","author":"Kwart","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.micpath.2019.103706_bib20","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/nature12048","article-title":"A CRISPR\/Cas system mediates bacterial innate immune evasion and virulence","volume":"497","author":"Sampson","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2019.103706_bib21","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1128\/MMBR.00039-13","article-title":"The role of CRISPR-Cas systems in virulence of pathogenic bacteria","volume":"78","author":"Louwen","year":"2014","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.micpath.2019.103706_bib22","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s10096-012-1733-4","article-title":"A novel link between Campylobacter jejuni bacteriophage defence, virulence and Guillain-Barre syndrome","volume":"32","author":"Louwen","year":"2013","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"10.1016\/j.micpath.2019.103706_bib23","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1038\/cr.2016.135","article-title":"Type I CRISPR-Cas targets endogenous genes and regulates virulence to evade mammalian host immunity","volume":"26","author":"Li","year":"2016","journal-title":"Cell Res."},{"key":"10.1016\/j.micpath.2019.103706_bib24","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1128\/JB.01445-10","article-title":"Genome sequence of poultry pathogen Riemerella anatipestifer strain RA-YM","volume":"193","author":"Zhou","year":"2011","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.micpath.2019.103706_bib25","doi-asserted-by":"crossref","first-page":"688","DOI":"10.3389\/fmicb.2017.00688","article-title":"A novel RAYM_RS09735\/RAYM_RS09740 two-component signaling system regulates gene expression and virulence in Riemerella anatipestifer","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib26","doi-asserted-by":"crossref","first-page":"382","DOI":"10.3389\/fcimb.2017.00382","article-title":"The role of the regulator Fur in gene regulation and virulence of Riemerella anatipestifer assessed using an unmarked gene deletion system","volume":"7","author":"Guo","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib27","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.vetmic.2015.03.003","article-title":"The M949_1556 gene plays a role on the bacterial antigenicity and pathogenicity of Riemerella anatipestifer","volume":"177","author":"Zou","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib28","doi-asserted-by":"crossref","DOI":"10.1128\/genomeA.00278-17","article-title":"Complete genome sequence of Riemerella anatipestifer serotype 10 strain HXb2","volume":"5","author":"Hu","year":"2017","journal-title":"Genome Announc."},{"key":"10.1016\/j.micpath.2019.103706_bib29","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1016\/j.cell.2009.07.040","article-title":"RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex","volume":"139","author":"Hale","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.micpath.2019.103706_bib30","doi-asserted-by":"crossref","first-page":"E2579","DOI":"10.1073\/pnas.1208507109","article-title":"Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria","volume":"109","author":"Gasiunas","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.micpath.2019.103706_bib31","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1038\/ncomms1937","article-title":"Molecular memory of prior infections activates the CRISPR\/Cas adaptive bacterial immunity system","volume":"3","author":"Datsenko","year":"2012","journal-title":"Nat. Commun."},{"key":"10.1016\/j.micpath.2019.103706_bib32","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1038\/nature09886","article-title":"CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III","volume":"471","author":"Deltcheva","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2019.103706_bib33","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/41483","article-title":"The complete genome sequence of the gastric pathogen Helicobacter pylori","volume":"388","author":"Tomb","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2019.103706_bib34","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1042\/BST20130163","article-title":"Cas9-dependent endogenous gene regulation is required for bacterial virulence","volume":"41","author":"Sampson","year":"2013","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.micpath.2019.103706_bib35","doi-asserted-by":"crossref","first-page":"2577","DOI":"10.1093\/nar\/gkt1074","article-title":"Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems","volume":"42","author":"Fonfara","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2019.103706_bib36","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0167-5699(99)01468-1","article-title":"Regional specialization in the mucosal immune system: primed cells do not always home along the same track","volume":"20","author":"Brandtzaeg","year":"1999","journal-title":"Immunol. Today"},{"key":"10.1016\/j.micpath.2019.103706_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2009.01659.x","article-title":"Peptidoglycan-associated lipoprotein (Pal) of Gram-negative bacteria: function, structure, role in pathogenesis and potential application in immunoprophylaxis","volume":"298","author":"Godlewska","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.micpath.2019.103706_bib38","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1128\/CMR.12.1.19","article-title":"Role of heat shock proteins in protection from and pathogenesis of infectious diseases","volume":"12","author":"Zugel","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.micpath.2019.103706_bib39","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF00548934","article-title":"Stress proteins and the immune response","volume":"58","author":"Young","year":"1990","journal-title":"Antonie Leeuwenhoek"},{"key":"10.1016\/j.micpath.2019.103706_bib40","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1128\/IAI.01391-13","article-title":"Mucosal immunization with recombinant fusion protein DnaJ-DeltaA146Ply enhances cross-protective immunity against Streptococcus pneumoniae infection in mice via interleukin 17A","volume":"82","author":"Liu","year":"2014","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2019.103706_bib41","doi-asserted-by":"crossref","first-page":"8419","DOI":"10.1128\/JVI.01020-13","article-title":"Massive activation of archaeal defense genes during viral infection","volume":"87","author":"Quax","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2019.103706_bib42","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0038077","article-title":"Phage-induced expression of CRISPR-associated proteins is revealed by shotgun proteomics in Streptococcus thermophilus","volume":"7","author":"Young","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.micpath.2019.103706_bib43","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00074-13","article-title":"The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae","volume":"4","author":"Gunderson","year":"2013","journal-title":"mBio"},{"key":"10.1016\/j.micpath.2019.103706_bib44","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1111\/j.1365-2958.2010.07482.x","article-title":"Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in Escherichia coli","volume":"79","author":"Perez-Rodriguez","year":"2011","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib45","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1126\/science.1068408","article-title":"Molecular chaperones in the cytosol: from nascent chain to folded protein","volume":"295","author":"Hartl","year":"2002","journal-title":"Science (New York, NY)"},{"key":"10.1016\/j.micpath.2019.103706_bib46","first-page":"1570","article-title":"Deletion of the small RNA chaperone protein Hfq down regulates genes related to virulence and confers protection against wild-type Brucella challenge in mice","volume":"6","author":"Lei","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2019.103706_bib47","doi-asserted-by":"crossref","first-page":"279","DOI":"10.5604\/01.3001.0009.2123","article-title":"Chaperone DnaJ influences the formation of biofilm by Escherichia coli","volume":"64","author":"Grudniak","year":"2015","journal-title":"Pol. J. Microbiol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019301706?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019301706?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:24:35Z","timestamp":1760109875000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401019301706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":47,"alternative-id":["S0882401019301706"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103706","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2019.103706","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"103706"}}