{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T18:31:37Z","timestamp":1778610697770,"version":"3.51.4"},"reference-count":72,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100008257","name":"Tarbiat Modares University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2020,5]]},"DOI":"10.1016\/j.micpath.2020.104079","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T22:44:32Z","timestamp":1582065872000},"page":"104079","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":24,"special_numbering":"C","title":["Structural predication and antigenic analysis of ROP16 protein utilizing immunoinformatics methods in order to identification of a vaccine against Toxoplasma gondii: An in silico approach"],"prefix":"10.1016","volume":"142","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9635-2876","authenticated-orcid":false,"given":"Ali Dalir","family":"Ghaffari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5591-5513","authenticated-orcid":false,"given":"Abdolhossein","family":"Dalimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0891-8214","authenticated-orcid":false,"given":"Fatemeh","family":"Ghaffarifar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0046-4772","authenticated-orcid":false,"given":"Majid","family":"Pirestani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2020.104079_bib1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.micpath.2020.104079_bib2","first-page":"153","article-title":"Molecular identification of Toxoplasma gondii in the native slaughtered cattle of Tehran Province, Iran","volume":"6","author":"Ghaffari","year":"2019","journal-title":"J. Food Qual. Hazards Control"},{"key":"10.1016\/j.micpath.2020.104079_bib3","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.4315\/0362-028X.JFP-11-417","article-title":"Annual cost of illness and quality-adjusted life year losses in the United States due to 14 foodborne pathogens","volume":"75","author":"Hoffmann","year":"2012","journal-title":"J. Food Protect."},{"key":"10.1016\/j.micpath.2020.104079_bib4","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1093\/bfgp\/elr028","article-title":"Genetic approaches for understanding virulence in Toxoplasma gondii","volume":"10","author":"Weilhammer","year":"2011","journal-title":"Brief. Funct. Genom."},{"key":"10.1016\/j.micpath.2020.104079_bib5","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1128\/MMBR.64.3.607-623.2000","article-title":"Lytic cycle of Toxoplasma gondii","volume":"64","author":"Black","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.micpath.2020.104079_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S1462399401002277","article-title":"Parasite\u2013host cell interactions in toxoplasmosis: new avenues for intervention?","volume":"3","author":"Coppens","year":"2001","journal-title":"Expet Rev. Mol. Med."},{"key":"10.1016\/j.micpath.2020.104079_bib7","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1111\/imm.12643","article-title":"Transnuclear CD 8 T cells specific for the immunodominant epitope Gra6 lower acute\u2010phase Toxoplasma gondii burden","volume":"149","author":"Sanecka","year":"2016","journal-title":"Immunology"},{"key":"10.1016\/j.micpath.2020.104079_bib8","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.exppara.2012.09.015","article-title":"Toxoplasma gondii: bioinformatics analysis, cloning and expression of a novel protein TgIMP1","volume":"132","author":"Bai","year":"2012","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib9","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.meegid.2018.04.033","article-title":"Bioinformatics analysis of ROP8 protein to improve vaccine design against Toxoplasma gondii","volume":"62","author":"Foroutan","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.micpath.2020.104079_bib10","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.4161\/hv.21006","article-title":"Vaccines against Toxoplasma gondii: status, challenges and future directions","volume":"8","author":"Liu","year":"2012","journal-title":"Hum. Vaccines Immunother."},{"key":"10.1016\/j.micpath.2020.104079_bib11","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1586\/erv.10.113","article-title":"Vaccination against Toxoplasma gondii: an increasing priority for collaborative research?","volume":"9","author":"Innes","year":"2010","journal-title":"Expet Rev. Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib12","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.biopha.2016.05.041","article-title":"Human toxoplasmosis\u2013Searching for novel chemotherapeutics","volume":"82","author":"Antczak","year":"2016","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.micpath.2020.104079_bib13","article-title":"A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization","author":"Rezaei","year":"2018","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2020.104079_bib14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1186\/1756-3305-5-273","article-title":"Evaluation of protective immune responses induced by DNA vaccines encoding Toxoplasma gondii surface antigen 1 (SAG1) and 14-3-3 protein in BALB\/c mice","volume":"5","author":"Meng","year":"2012","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.micpath.2020.104079_bib15","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1016\/S0264-410X(03)00163-4","article-title":"DNA vaccination with the immunodominant tachyzoite surface antigen (SAG-1) protects against adult acquired Toxoplasma gondii infection but does not prevent maternofoetal transmission","volume":"21","author":"Couper","year":"2003","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib16","first-page":"704","article-title":"Recombinant GRA4 or ROP2 protein combined with alum or the gra4 gene provides partial protection in chronic murine models of toxoplasmosis","volume":"11","author":"Martin","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.micpath.2020.104079_bib17","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nrmicro1800","article-title":"Kiss and spit: the dual roles of Toxoplasma rhoptries","volume":"6","author":"Boothroyd","year":"2008","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2020.104079_bib18","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1126\/science.1133690","article-title":"Polymorphic secreted kinases are key virulence factors in toxoplasmosis","volume":"314","author":"Saeij","year":"2006","journal-title":"Science"},{"key":"10.1016\/j.micpath.2020.104079_bib19","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.mib.2007.09.013","article-title":"Rhoptries: an arsenal of secreted virulence factors","volume":"10","author":"Bradley","year":"2007","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.micpath.2020.104079_bib20","series-title":"Toxoplasma Rhoptries: Unique Secretory Organelles and Source of Promising Vaccine Proteins for Immunoprevention of Toxoplasmosis","first-page":"2008","author":"Dlugonska","year":"2008"},{"key":"10.1016\/j.micpath.2020.104079_bib21","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1111\/j.1462-5822.2007.00909.x","article-title":"Rhoptries are major players in Toxoplasma gondii invasion and host cell interaction","volume":"9","author":"Dubremetz","year":"2007","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.micpath.2020.104079_bib22","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1515\/ap-2016-0042","article-title":"Bioinformatics analysis and expression of a novel protein ROP48 in Toxoplasma gondii","volume":"61","author":"Zhou","year":"2016","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib23","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1016\/j.micinf.2012.09.005","article-title":"Virulence factors of Toxoplasma gondii","volume":"14","author":"Dubremetz","year":"2012","journal-title":"Microb. Infect."},{"key":"10.1016\/j.micpath.2020.104079_bib24","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1146\/annurev-micro-091014-104353","article-title":"Genetic mapping of pathogenesis determinants in Toxoplasma gondii","volume":"70","author":"Behnke","year":"2016","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2020.104079_bib25","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1128\/IAI.05974-11","article-title":"An inside job: hacking into Janus kinase\/signal transducer and activator of transcription signaling cascades by the intracellular protozoan Toxoplasma gondii","volume":"80","author":"Denkers","year":"2012","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2020.104079_bib26","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.micpath.2019.05.013","article-title":"In silico analysis and expression of a novel chimeric antigen as a vaccine candidate against Toxoplasma gondii","volume":"132","author":"Dodangeh","year":"2019","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2020.104079_bib27","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/s12639-018-0996-9","article-title":"Bioinformatics analysis of single and multi-hybrid epitopes of GRA-1, GRA-4, GRA-6 and GRA-7 proteins to improve DNA vaccine design against Toxoplasma gondii","volume":"42","author":"Shaddel","year":"2018","journal-title":"J. Parasit. Dis."},{"key":"10.1016\/j.micpath.2020.104079_bib28","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1093\/bib\/bbr055","article-title":"Tools and collaborative environments for bioinformatics research","volume":"12","author":"Romano","year":"2011","journal-title":"Briefings Bioinf."},{"key":"10.1016\/j.micpath.2020.104079_bib29","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s00436-015-4824-1","article-title":"Review on the identification and role of Toxoplasma gondii antigenic epitopes","volume":"115","author":"Wang","year":"2016","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.micpath.2020.104079_bib30","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.exppara.2013.09.019","article-title":"Identification of novel B cell epitopes within Toxoplasma gondii GRA1","volume":"135","author":"Wang","year":"2013","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib31","series-title":"Protein Identification and Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook","first-page":"571","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.micpath.2020.104079_bib32","series-title":"[32] GOR Method for Predicting Protein Secondary Structure from Amino Acid Sequence. Methods in Enzymology","first-page":"540","author":"Garnier","year":"1996"},{"key":"10.1016\/j.micpath.2020.104079_bib33","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/bioinformatics\/16.4.404","article-title":"The PSIPRED protein structure prediction server","volume":"16","author":"McGuffin","year":"2000","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2020.104079_bib34","doi-asserted-by":"crossref","first-page":"41","DOI":"10.7774\/cevr.2016.5.1.41","article-title":"In silico analysis of an envelope domain III-based multivalent fusion protein as a potential dengue vaccine candidate","volume":"5","author":"Fahimi","year":"2016","journal-title":"Clin. Exp. Vaccine Res."},{"key":"10.1016\/j.micpath.2020.104079_bib35","doi-asserted-by":"crossref","first-page":"W230","DOI":"10.1093\/nar\/gki412","article-title":"DiANNA: a web server for disulfide connectivity prediction","volume":"33","author":"Ferr\u00e8","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2020.104079_bib36","doi-asserted-by":"crossref","first-page":"S162","DOI":"10.1002\/elps.200900140","article-title":"Automated comparative protein structure modeling with SWISS\u2010MODEL and Swiss\u2010PdbViewer: a historical perspective","volume":"30","author":"Guex","year":"2009","journal-title":"Electrophoresis"},{"key":"10.1016\/j.micpath.2020.104079_bib37","doi-asserted-by":"crossref","first-page":"1974","DOI":"10.1002\/prot.24086","article-title":"Refinement of unreliable local regions in template\u2010based protein models","volume":"80","author":"Park","year":"2012","journal-title":"Proteins: Struct. Funct. Bioinf."},{"key":"10.1016\/j.micpath.2020.104079_bib38","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-09654-8","article-title":"Modeling protein quaternary structure of homo-and hetero-oligomers beyond binary interactions by homology","volume":"7","author":"Bertoni","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2020.104079_bib39","doi-asserted-by":"crossref","first-page":"W407","DOI":"10.1093\/nar\/gkm290","article-title":"ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins","volume":"35","author":"Wiederstein","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2020.104079_bib40","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1080\/20477724.2018.1446773","article-title":"Current progress of immunoinformatics approach harnessed for cellular-and antibody-dependent vaccine design","volume":"112","author":"Kazi","year":"2018","journal-title":"Pathog. Glob. Health"},{"key":"10.1016\/j.micpath.2020.104079_bib41","series-title":"International Conference on Artificial Immune Systems","first-page":"197","article-title":"BcePred: prediction of continuous B-cell epitopes in antigenic sequences using physico-chemical properties","author":"Saha","year":"2004"},{"key":"10.1016\/j.micpath.2020.104079_bib42","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B\u2010cell epitopes in an antigen using recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins: Struct. Funct. Bioinf."},{"key":"10.1016\/j.micpath.2020.104079_bib43","doi-asserted-by":"crossref","first-page":"5425","DOI":"10.1021\/bi00367a013","article-title":"New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites","volume":"25","author":"Parker","year":"1986","journal-title":"Biochemistry"},{"key":"10.1016\/j.micpath.2020.104079_bib44","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1745-7580-2-2","article-title":"Improved method for predicting linear B-cell epitopes","volume":"2","author":"Larsen","year":"2006","journal-title":"Immunome Res."},{"key":"10.1016\/j.micpath.2020.104079_bib45","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0014-5793(90)80535-Q","article-title":"A semi-empirical method for prediction of antigenic determinants on protein antigens","volume":"276","author":"Kolaskar","year":"1990","journal-title":"FEBS Lett."},{"key":"10.1016\/j.micpath.2020.104079_bib46","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/jvi.55.3.836-839.1985","article-title":"Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide","volume":"55","author":"Emini","year":"1985","journal-title":"J. Virol."},{"key":"10.1016\/j.micpath.2020.104079_bib47","doi-asserted-by":"crossref","unstructured":"Chou P, Fasman G. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. 47: 45\u2013148. Chou4547Adv Enzymol. 1978.","DOI":"10.1002\/9780470122921.ch2"},{"key":"10.1016\/j.micpath.2020.104079_bib48","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1007\/BF01195768","article-title":"Prediction of chain flexibility in proteins","volume":"72","author":"Karplus","year":"1985","journal-title":"Naturwissenschaften"},{"key":"10.1016\/j.micpath.2020.104079_bib49","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1002829","article-title":"Reliable B cell epitope predictions: impacts of method development and improved benchmarking","volume":"8","author":"Kringelum","year":"2012","journal-title":"PLoS Comput. Biol."},{"key":"10.1016\/j.micpath.2020.104079_bib50","doi-asserted-by":"crossref","first-page":"3195","DOI":"10.1016\/j.vaccine.2004.02.005","article-title":"Prediction of CTL epitopes using QM, SVM and ANN techniques","volume":"22","author":"Bhasin","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib51","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jtbi.2014.01.018","article-title":"A novel multi-epitope peptide vaccine against cancer: an in silico approach","volume":"349","author":"Nezafat","year":"2014","journal-title":"J. Theor. Biol."},{"key":"10.1016\/j.micpath.2020.104079_bib52","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","article-title":"High-throughput prediction of protein antigenicity using protein microarray data","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2020.104079_bib53","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1471-2105-8-4","article-title":"VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines","volume":"8","author":"Doytchinova","year":"2007","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.micpath.2020.104079_bib54","doi-asserted-by":"crossref","first-page":"W202","DOI":"10.1093\/nar\/gkl343","article-title":"AlgPred: prediction of allergenic proteins and mapping of IgE epitopes","volume":"34","author":"Saha","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2020.104079_bib55","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1093\/bioinformatics\/btp386","article-title":"SOLpro: accurate sequence-based prediction of protein solubility","volume":"25","author":"Magnan","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2020.104079_bib56","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.vetpar.2017.07.013","article-title":"The global seroprevalence of Toxoplasma gondii among wild boars: a systematic review and meta-analysis","volume":"244","author":"Rostami","year":"2017","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib57","first-page":"389","article-title":"Toxoplasma gondii infection in immunocompromised patients: a systematic review and meta-analysis","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2020.104079_bib58","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1586\/erv.09.27","article-title":"Current status of toxoplasmosis vaccine development","volume":"8","author":"Kur","year":"2009","journal-title":"Expet Rev. Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib59","first-page":"235","article-title":"Immunogenicity and antigen structure","author":"Berzofsky","year":"1993","journal-title":"Fundamental Immunol."},{"key":"10.1016\/j.micpath.2020.104079_bib60","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1186\/1756-0500-2-111","article-title":"A comprehensive resource for integrating and displaying protein post-translational modifications","volume":"2","author":"Lee","year":"2009","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.micpath.2020.104079_bib61","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1515\/ap-2017-0061","article-title":"Structural and antigenic analysis of a new rhoptry pseudokinase gene (ROP54) in Toxoplasma gondii","volume":"62","author":"Zhou","year":"2017","journal-title":"Acta Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib62","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.mgene.2015.07.007","article-title":"Structural modeling and in silico analysis of non-synonymous single nucleotide polymorphisms of human 3\u03b2-hydroxysteroid dehydrogenase type 2","volume":"5","author":"Goswami","year":"2015","journal-title":"Meta Gene"},{"key":"10.1016\/j.micpath.2020.104079_bib63","first-page":"17","article-title":"T-cell immunity in human chronic toxoplasmosis","volume":"41","author":"El-Kady","year":"2011","journal-title":"J. Egypt. Soc. Parasitol."},{"key":"10.1016\/j.micpath.2020.104079_bib64","first-page":"71","article-title":"Immune response to Toxoplasma gondii","volume":"40","author":"Filisetti","year":"2004","journal-title":"Ann. Ist. Super Sanita"},{"key":"10.1016\/j.micpath.2020.104079_bib65","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0126334","article-title":"Prime-boost vaccination with Toxoplasma lysate antigen, but not with a mixture of recombinant protein antigens, leads to reduction of brain cyst formation in BALB\/c mice","volume":"10","author":"Wagner","year":"2015","journal-title":"PloS One"},{"key":"10.1016\/j.micpath.2020.104079_bib66","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.4049\/jimmunol.164.5.2629","article-title":"Decreased resistance of B cell-deficient mice to infection with Toxoplasma gondii despite unimpaired expression of IFN-\u03b3, TNF-\u03b1, and inducible nitric oxide synthase","volume":"164","author":"Kang","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2020.104079_bib67","doi-asserted-by":"crossref","first-page":"6757","DOI":"10.1016\/j.vaccine.2015.10.077","article-title":"Toxoplasma gondii: vaccination with a DNA vaccine encoding T-and B-cell epitopes of SAG1, GRA2, GRA7 and ROP16 elicits protection against acute toxoplasmosis in mice","volume":"33","author":"Cao","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib68","doi-asserted-by":"crossref","first-page":"3913","DOI":"10.1016\/j.vaccine.2008.04.046","article-title":"Multi-epitope DNA vaccine linked to the A2\/B subunit of cholera toxin protect mice against Toxoplasma gondii","volume":"26","author":"Cong","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2020.104079_bib69","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/1756-3305-7-145","article-title":"Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice","volume":"7","author":"Cong","year":"2014","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.micpath.2020.104079_bib70","doi-asserted-by":"crossref","DOI":"10.1051\/parasite\/2017013","article-title":"Vaccination with recombinant adenovirus expressing multi-stage antigens of Toxoplasma gondii by the mucosal route induces higher systemic cellular and local mucosal immune responses than with other vaccination routes","volume":"24","author":"Wang","year":"2017","journal-title":"Parasite"},{"key":"10.1016\/j.micpath.2020.104079_bib71","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1186\/s13071-015-1108-7","article-title":"A Toxoplasma gondii vaccine encoding multistage antigens in conjunction with ubiquitin confers protective immunity to BALB\/c mice against parasite infection","volume":"8","author":"Yin","year":"2015","journal-title":"Parasites Vectors"},{"key":"10.1016\/j.micpath.2020.104079_bib72","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1515\/ap-2018-0028","article-title":"Structural predication and antigenic analysis of Toxoplasma gondii ROP20","volume":"63","author":"Zhou","year":"2018","journal-title":"Acta Parasitol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019321692?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401019321692?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:30:24Z","timestamp":1760110224000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401019321692"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5]]},"references-count":72,"alternative-id":["S0882401019321692"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104079","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Structural predication and antigenic analysis of ROP16 protein utilizing immunoinformatics methods in order to identification of a vaccine against Toxoplasma gondii: An in silico approach","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104079","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104079"}}