{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:18:36Z","timestamp":1777429116690,"version":"3.51.4"},"reference-count":66,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1016\/j.micpath.2020.104283","type":"journal-article","created":{"date-parts":[[2020,5,30]],"date-time":"2020-05-30T11:20:12Z","timestamp":1590837612000},"page":"104283","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Biotechnological applications from a Leishmania amastigote-specific hypothetical protein in the canine and human visceral leishmaniasis"],"prefix":"10.1016","volume":"147","author":[{"given":"Jo\u00e3o A.","family":"Oliveira-da-Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda S.","family":"Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grasiele S.V.","family":"Tavares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda F.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela P.","family":"Lage","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Ludolf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bethina T.","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago A.R.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tha\u00eds T.O.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourena E.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel S.","family":"Bandeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edvian T.","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nath\u00e1lia C.","family":"Galvani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana T.","family":"Chaves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamil S.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel A.","family":"Ch\u00e1vez-Fumagalli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Una\u00ed","family":"Tupinamb\u00e1s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle F.","family":"de Magalh\u00e3es-Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia A.G.","family":"Silveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Lyon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo A.","family":"Machado-de-\u00c1vila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo A.F.","family":"Coelho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2020.104283_bib1","author":"World Health Organization"},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib2","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1128\/CMR.6.3.230","article-title":"Leishmaniases of the New World: current concepts and implications for future research","volume":"6","author":"Grimaldi","year":"1993","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.micpath.2020.104283_bib3","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0140-6736(18)31204-2","article-title":"Leishmaniasis","volume":"392","author":"Burza","year":"2018","journal-title":"Lancet"},{"key":"10.1016\/j.micpath.2020.104283_bib4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.cellimm.2017.11.001","article-title":"Recombinant prohibitin protein of Leishmania infantum acts as a vaccine candidate and diagnostic marker against visceral leishmaniasis","volume":"323","author":"Dias","year":"2018","journal-title":"Cell. Immunol."},{"issue":"4","key":"10.1016\/j.micpath.2020.104283_bib5","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s40291-018-0343-y","article-title":"Molecular diagnosis of visceral leishmaniasis","volume":"22","author":"Sundar","year":"2018","journal-title":"Mol. Diagn. Ther."},{"key":"10.1016\/j.micpath.2020.104283_bib6","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0071833","article-title":"Risk factors for seroconversion by Leishmania infantum in a cohort of dogs from an endemic area of Brazil","volume":"8","author":"Coura-Vital","year":"2013","journal-title":"PloS One"},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007216","article-title":"Determinants for progression from asymptomatic infection to symptomatic visceral leishmaniasis: a cohort study","volume":"13","author":"Chakravarty","year":"2019","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"2","key":"10.1016\/j.micpath.2020.104283_bib8","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.diagmicrobio.2019.04.015","article-title":"Diagnostic evaluation of the amastin protein from Leishmania infantum in canine and human visceral leishmaniasis and immunogenicity in human cells derived from patients and healthy controls","volume":"95","author":"Vale","year":"2019","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.micpath.2020.104283_bib9","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.rvsc.2019.08.010","article-title":"Diagnostic markers selected by immunoproteomics and phage display applied for the serodiagnosis of canine leishmaniosis","volume":"126","author":"Machado","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.micpath.2020.104283_bib10","doi-asserted-by":"crossref","DOI":"10.1590\/0074-02760180452","article-title":"Mproving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests","volume":"114","author":"Teixeira","year":"2019","journal-title":"Mem. Inst. Oswaldo Cruz"},{"issue":"2","key":"10.1016\/j.micpath.2020.104283_bib11","doi-asserted-by":"crossref","first-page":"200","DOI":"10.2174\/15672018113106660057","article-title":"New insights into the diagnosis and chemotherapy for visceral leishmaniasis","volume":"11","author":"Kumar","year":"2014","journal-title":"Curr. Drug Deliv."},{"key":"10.1016\/j.micpath.2020.104283_bib12","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3389\/fcimb.2018.00302","article-title":"Biomarkers associated with Leishmania infantum exposure, infection, and disease in dogs","volume":"8","author":"Maia","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"8","key":"10.1016\/j.micpath.2020.104283_bib13","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007658","article-title":"Systematic review on antigens for serodiagnosis of visceral leishmaniasis, with a focus on East Africa","volume":"13","author":"K\u00fchne","year":"2019","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib14","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4103\/0972-9062.179209","article-title":"Laboratory diagnosis of human visceral leishmaniasis","volume":"53","author":"Sakkas","year":"2016","journal-title":"J. Vector Borne Dis."},{"issue":"9","key":"10.1016\/j.micpath.2020.104283_bib15","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0184867","article-title":"Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis","volume":"12","author":"Magalh\u00e3es","year":"2017","journal-title":"PloS One"},{"issue":"10","key":"10.1016\/j.micpath.2020.104283_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006871","article-title":"High accuracy of an ELISA test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil - a phase II study","volume":"12","author":"Borja","year":"2018","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2020.104283_bib17","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.micpath.2017.06.020","article-title":"Antigenicity of phage clones and their synthetic peptides for the serodiagnosis of canine and human visceral leishmaniasis","volume":"110","author":"Costa","year":"2017","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2020.104283_bib18","doi-asserted-by":"crossref","first-page":"112641","DOI":"10.1016\/j.jim.2019.112641","article-title":"Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum prohibitin protein and a synthetic peptide containing its conformational B-cell epitope","author":"Rodrigues","year":"2019","journal-title":"J. Immunol. Methods"},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib19","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.imbio.2018.09.003","article-title":"Potential application of small myristoylated protein-3 evaluated as recombinant antigen and a synthetic peptide containing its linear B-cell epitope for the serodiagnosis of canine visceral and human tegumentary leishmaniasis","volume":"224","author":"Salles","year":"2019","journal-title":"Immunobiology"},{"key":"10.1016\/j.micpath.2020.104283_bib20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mib.2012.05.002","article-title":"Making an anti-amastigote vaccine for visceral leishmaniasis: rational, update and perspectives","volume":"15","author":"Fernandes","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.micpath.2020.104283_bib21","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0732-8893(02)00410-8","article-title":"Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen","volume":"43","author":"Carvalho","year":"2002","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib22","doi-asserted-by":"crossref","first-page":"e2148","DOI":"10.1371\/journal.pntd.0002148","article-title":"Antigenicity and protective efficacy of a Leishmania amastigote-specific protein, member of the super-oxygenase family, against visceral leishmaniasis","volume":"7","author":"Martins","year":"2013","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"4","key":"10.1016\/j.micpath.2020.104283_bib23","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1007\/s00436-016-4904-x","article-title":"A new Leishmania-specific hypothetical protein and its non-described specific B cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis","volume":"115","author":"Lage","year":"2016","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.micpath.2020.104283_bib24","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3389\/fimmu.2017.00227","article-title":"Leishmania donovani nucleoside hydrolase (NH36) domains induce T-cell cytokine responses in human visceral leishmaniasis","volume":"8","author":"Barbosa-Santos","year":"2017","journal-title":"Front. Immunol."},{"issue":"4","key":"10.1016\/j.micpath.2020.104283_bib25","first-page":"904","article-title":"Role of immune cells and cytokines for immune response in Kala-Azar","volume":"27","author":"Sarkar","year":"2018","journal-title":"Mymensingh Med. J."},{"issue":"2","key":"10.1016\/j.micpath.2020.104283_bib26","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0117977","article-title":"The involvement of TLR2 and TLR4 in cytokine and nitric oxide production in visceral leishmaniasis patients before and after treatment with anti-leishmanial drugs","volume":"10","author":"Gatto","year":"2015","journal-title":"PloS One"},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004468","article-title":"Successful treatment of human visceral leishmaniasis restores antigen-specific IFN-\u03b3, but not IL-10 production","volume":"10","author":"Adem","year":"2016","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib28","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1590\/S0100-879X2010007500134","article-title":"Anti-Leishmania titers and positive skin tests in patients cured of kala-azar","volume":"44","author":"Viana","year":"2011","journal-title":"Braz. J. Med. Biol. Res."},{"key":"10.1016\/j.micpath.2020.104283_bib29","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3389\/fcimb.2018.00326","article-title":"In search of biomarkers for pathogenesis and control of leishmaniasis by global analyses of Leishmania-infected macrophages","volume":"8","author":"Veras","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2020.104283_bib30","doi-asserted-by":"crossref","first-page":"670","DOI":"10.3389\/fimmu.2019.00670","article-title":"Cytokines: key determinants of resistance or disease progression in visceral leishmaniasis: opportunities for novel diagnostics and immunotherapy","volume":"10","author":"Dayakar","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2020.104283_bib31","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1086\/588387","article-title":"Increased levels of interleukin-10 and IgG3 are hallmarks of Indian post-kala-azar dermal leishmaniasis","volume":"197","author":"Ganguly","year":"2008","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.micpath.2020.104283_bib32","doi-asserted-by":"crossref","first-page":"e3273","DOI":"10.1371\/journal.pntd.0003273","article-title":"IgG1 as a potential biomarker of post-chemotherapeutic relapse in visceral leishmaniasis, and adaptation to a rapid diagnostic test","volume":"8","author":"Bhattacharyya","year":"2014","journal-title":"PLoS Neglected Trop. Dis."},{"issue":"11","key":"10.1016\/j.micpath.2020.104283_bib33","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1017\/S0031182019000714","article-title":"Screening diagnostic candidates from Leishmania infantum proteins for human visceral leishmaniasis using an immunoproteomics approach","volume":"146","author":"Lage","year":"2019","journal-title":"Parasitology"},{"key":"10.1016\/j.micpath.2020.104283_bib34","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.1128\/IAI.71.7.3988-3994.2003","article-title":"Immune responses induced by the Leishmania (Leishmania) donovani A2 antigen, but not by the LACK antigen, are protective against experimental Leishmania (Leishmania) amazonensis infection","volume":"71","author":"Coelho","year":"2003","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2020.104283_bib35","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib36","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/ng0393-266","article-title":"Identification of protein coding regions by database similarity search","volume":"3","author":"Gish","year":"1993","journal-title":"Nat. Genet."},{"key":"10.1016\/j.micpath.2020.104283_bib37","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.micpath.2020.104283_bib38","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B-cell epitopes in an antigen using recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins"},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib39","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/0042-6822(85)90441-6","article-title":"Antigenic conservation and divergence between the viral-specific proteins of poliovirus type 1 and various picornaviruses","volume":"140","author":"Emini","year":"1985","journal-title":"Virology"},{"key":"10.1016\/j.micpath.2020.104283_bib40","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0166-6851(96)02612-6","article-title":"Identification and overexpression of the A2 amastigote-specific protein in Leishmania donovani","volume":"78","author":"Zhang","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"issue":"6","key":"10.1016\/j.micpath.2020.104283_bib41","first-page":"CD009135","article-title":"Rapid tests for the diagnosis of visceral leishmaniasis in patients with suspected disease","author":"Boelaert","year":"2014","journal-title":"Cochrane Database Syst. Rev."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib42","first-page":"128","article-title":"Application of recombinant proteins for serodiagnosis of visceral leishmaniasis in humans and dogs","volume":"20","author":"Farahmand","year":"2016","journal-title":"Iran. Biomed. J."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0091009","article-title":"Evaluation of change in canine diagnosis protocol adopted by the visceral leishmaniasis control program in Brazil and a new proposal for diagnosis","volume":"9","author":"Coura-Vital","year":"2014","journal-title":"PloS One"},{"key":"10.1016\/j.micpath.2020.104283_bib44","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.cellimm.2017.06.001","article-title":"An ELISA immunoassay employing a conserved Leishmania hypothetical protein for the serodiagnosis of visceral and tegumentary leishmaniasis in dogs and humans","volume":"318","author":"Carvalho","year":"2017","journal-title":"Cell. Immunol."},{"issue":"17","key":"10.1016\/j.micpath.2020.104283_bib45","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1007\/s00216-018-1067-x","article-title":"A versatile and sensitive lateral flow immunoassay for the rapid diagnosis of visceral leishmaniasis","volume":"410","author":"Anfossi","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"10.1016\/j.micpath.2020.104283_bib46","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-019-10068-x","article-title":"Multiplex flow cytometry serology to diagnosis of canine visceral leishmaniasis","author":"Ker","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"9","key":"10.1016\/j.micpath.2020.104283_bib47","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1128\/CVI.00257-12","article-title":"Evaluation of an enzyme-linked immunosorbent assay based on crude Leishmania histone proteins for serodiagnosis of human infantile visceral leishmaniasis","volume":"19","author":"Lakhal","year":"2012","journal-title":"Clin. Vaccine Immunol."},{"issue":"5","key":"10.1016\/j.micpath.2020.104283_bib48","doi-asserted-by":"crossref","first-page":"860","DOI":"10.4269\/ajtmh.13-0623","article-title":"Recombinant Leishmania infantum heat shock protein 83 for the serodiagnosis of cutaneous, mucosal, and visceral leishmaniases","volume":"90","author":"Celeste","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib49","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1590\/S1984-29612015060","article-title":"Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in canine visceral leishmaniasis serological test","volume":"24","author":"Jusi","year":"2015","journal-title":"Rev. Bras. Parasitol. Vet."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib50","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1080\/20477724.2019.1616952","article-title":"An immunoproteomic approach to identifying immunoreactive proteins in Leishmania infantum amastigotes using sera of dogs infected with canine visceral leishmaniasis","volume":"113","author":"Rashidi","year":"2019","journal-title":"Pathog. Glob. Health"},{"issue":"5","key":"10.1016\/j.micpath.2020.104283_bib51","first-page":"2975","article-title":"Developmental gene expression in Leishmania donovani: differential cloning and analysis of an amastigote-stage-specific gene","volume":"14","author":"Charest","year":"1994","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.micpath.2020.104283_bib52","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.vetpar.2015.11.006","article-title":"Evaluation of two recombinant Leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis","volume":"215","author":"Coelho","year":"2016","journal-title":"Vet. Parasitol."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib53","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.parint.2018.02.001","article-title":"Serological diagnosis and prognostic of tegumentary and visceral leishmaniasis using a conserved Leishmania hypothetical protein","volume":"67","author":"Dias","year":"2018","journal-title":"Parasitol. Int."},{"issue":"10","key":"10.1016\/j.micpath.2020.104283_bib54","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0006871","article-title":"High accuracy of an ELISA test based in a flagella antigen of Leishmania in serodiagnosis of canine visceral leishmaniasis with potential to improve the control measures in Brazil - a phase II study","volume":"12","author":"Borja","year":"2018","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2020.104283_bib55","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.vetpar.2018.07.011","article-title":"Comparative analysis of the Leishmania infantum-specific antibody repertoires and the autoantibody repertoires between asymptomatic and symptomatic dogs","volume":"261","author":"Chaabouni","year":"2018","journal-title":"Vet. Parasitol."},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib56","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1186\/s12879-018-3420-2","article-title":"Development of a sandwich ELISA to detect Leishmania 40S ribosomal protein S12 antigen from blood samples of visceral leishmaniasis patients","volume":"18","author":"Zhang","year":"2018","journal-title":"BMC Infect. Dis."},{"issue":"2","key":"10.1016\/j.micpath.2020.104283_bib57","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.clim.2005.06.015","article-title":"T cell subset and cytokine profiles in human visceral leishmaniasis during active and asymptomatic or sub-clinical infection with Leishmania donovani","volume":"117","author":"Hailu","year":"2005","journal-title":"Clin. Immunol."},{"issue":"1","key":"10.1016\/j.micpath.2020.104283_bib58","doi-asserted-by":"crossref","first-page":"4334","DOI":"10.1038\/s41598-017-04595-8","article-title":"Anti-parasite therapy drives changes in human visceral leishmaniasis-associated inflammatory balance","volume":"7","author":"Ara\u00fajo-Santos","year":"2017","journal-title":"Sci. Rep."},{"issue":"10","key":"10.1016\/j.micpath.2020.104283_bib59","first-page":"1549","article-title":"Role of cytokines in the pathogenesis of visceral leishmaniasis","volume":"63","author":"Kumar","year":"2017","journal-title":"Clin. Lab."},{"key":"10.1016\/j.micpath.2020.104283_bib60","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.molimm.2017.09.017","article-title":"Recombinant small glutamine-rich tetratricopeptide repeat-containing protein of Leishmania infantum: potential vaccine and diagnostic application against visceral leishmaniasis","volume":"91","author":"Dias","year":"2017","journal-title":"Mol. Immunol."},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib61","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.imbio.2017.10.043","article-title":"Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis","volume":"223","author":"Portela","year":"2018","journal-title":"Immunobiology"},{"key":"10.1016\/j.micpath.2020.104283_bib62","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.cellimm.2018.09.006","article-title":"Diagnostic application of recombinant Leishmania proteins and evaluation of their in vitro immunogenicity after stimulation of immune cells collected from tegumentary leishmaniasis patients and healthy individuals","volume":"334","author":"Lima","year":"2018","journal-title":"Cell. Immunol."},{"issue":"2","key":"10.1016\/j.micpath.2020.104283_bib63","doi-asserted-by":"crossref","first-page":"e2675","DOI":"10.1371\/journal.pntd.0002675","article-title":"Significantly lower anti-Leishmania IgG responses in Sudanese versus Indian visceral leishmaniasis","volume":"8","author":"Bhattacharyya","year":"2014","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.micpath.2020.104283_bib64","doi-asserted-by":"crossref","first-page":"427","DOI":"10.3389\/fcimb.2018.00427","article-title":"Visceral leishmaniasis IgG1 rapid monitoring of cure vs. relapse, and potential for diagnosis of post kala-azar dermal leishmaniasis","volume":"8","author":"Marlais","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"6","key":"10.1016\/j.micpath.2020.104283_bib65","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0099216","article-title":"Mapping B-cell epitopes for the peroxidoxin of Leishmania (Viannia) braziliensis and its potential for the clinical diagnosis of tegumentary and visceral leishmaniasis","volume":"9","author":"Menezes-Souza","year":"2014","journal-title":"PloS One"},{"issue":"3","key":"10.1016\/j.micpath.2020.104283_bib66","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/sji.12265","article-title":"Anti-citrullinated peptide antibodies in Sudanese patients with Leishmania donovani infection exhibit reactivity not dependent on citrullination","volume":"81","author":"\u00c5hlin","year":"2015","journal-title":"Scand. J. Immunol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020306495?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020306495?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:31:25Z","timestamp":1760110285000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401020306495"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":66,"alternative-id":["S0882401020306495"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104283","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2020,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Biotechnological applications from a Leishmania amastigote-specific hypothetical protein in the canine and human visceral leishmaniasis","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104283","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104283"}}