{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:06:34Z","timestamp":1785812794505,"version":"3.56.0"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100007087","name":"University of Isfahan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1016\/j.micpath.2020.104555","type":"journal-article","created":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T12:37:24Z","timestamp":1601469444000},"page":"104555","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Inhibition of quorum sensing-controlled virulence factors with natural substances and novel protease, obtained from Halobacillus karajensis"],"prefix":"10.1016","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3517-1331","authenticated-orcid":false,"given":"Fatemeh S.","family":"Ghoreishi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1951-7627","authenticated-orcid":false,"given":"Rasoul","family":"Roghanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3879-9076","authenticated-orcid":false,"given":"Giti","family":"Emtiazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2020.104555_bib1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.micpath.2014.07.008","article-title":"Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa","volume":"74","author":"El-Mowafy","year":"2014","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.micpath.2020.104555_bib2","first-page":"99","article-title":"Inhibition of quorum sensing-controlled biofilm formation in Pseudomonas aeruginosa by quorum-sensing inhibitors. microb","volume":"111","author":"Kalaiarasan","year":"2017","journal-title":"Pathogens"},{"key":"10.1016\/j.micpath.2020.104555_bib3","doi-asserted-by":"crossref","first-page":"2330","DOI":"10.1080\/14786419.2014.934239","article-title":"Anti-quorum sensing activity of selected sponge extracts: a case study of Pseudomonas aeruginosa","volume":"28","author":"Pejin","year":"2014","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.micpath.2020.104555_bib4","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1080\/14786419.2014.945088","article-title":"In vitro anti-quorum sensing activity of phytol","volume":"29","author":"Pejin","year":"2015","journal-title":"Nat. Prod. Res."},{"key":"10.1016\/j.micpath.2020.104555_bib5","first-page":"1795","article-title":"The arsenal of pathogens and antivirulence therapeutic strategies for disarming them","volume":"10","author":"Brannon","year":"2016","journal-title":"Drug Des. Dev. Ther."},{"key":"10.1016\/j.micpath.2020.104555_bib6","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.3762\/bjoc.12.137","article-title":"Discovery of an inhibitor of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells","volume":"12","author":"Morkunas","year":"2016","journal-title":"Beilstein J. Org. Chem."},{"key":"10.1016\/j.micpath.2020.104555_bib7","doi-asserted-by":"crossref","first-page":"8452","DOI":"10.1039\/c2ob26501j","article-title":"Inhibition of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells by quorum sensing autoinducer-mimics","volume":"10","author":"Morkunas","year":"2012","journal-title":"Org. Biomol. Chem."},{"key":"10.1016\/j.micpath.2020.104555_bib8","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3390\/md8030705","article-title":"Production of metabolites as bacterial responses to the marine environment","volume":"8","author":"De Carvalho","year":"2010","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.micpath.2020.104555_bib9","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1099\/jmm.0.000206","article-title":"Inhibition of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa by human serum paraoxonase","volume":"65","author":"Aybey","year":"2016","journal-title":"J. Med. Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib10","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.micpath.2017.09.046","article-title":"Melittin and its potential in the destruction and inhibition of the biofilm formation by Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa isolated from bovine milk","volume":"112","author":"Picoli","year":"2017","journal-title":"Mic. pathog."},{"key":"10.1016\/j.micpath.2020.104555_bib11","doi-asserted-by":"crossref","first-page":"733","DOI":"10.2174\/1389201016666150505121951","article-title":"Quercetin potently reduces biofilm formation of the strain Pseudomonas aeruginosa PAO1 in vitro","volume":"16","author":"Pejin","year":"2015","journal-title":"Curr. Pharmaceut. Biotechnol."},{"key":"10.1016\/j.micpath.2020.104555_bib12","first-page":"1515","article-title":"Protease and amylase enzymes for biofilm removal and degradation of extracellular polymeric substances (EPS) produced by Pseudomonas fluorescens bacteria","volume":"4","author":"Molobela","year":"2010","journal-title":"Afr. J. Microbiol. Res."},{"key":"10.1016\/j.micpath.2020.104555_bib13","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1039\/c0md00270d","article-title":"Inhibition of Pseudomonas aeruginosa biofilms with a glycopeptide dendrimer containing D-amino acids","volume":"2","author":"Emma","year":"2011","journal-title":"Med. Chem. Comm."},{"key":"10.1016\/j.micpath.2020.104555_bib14","first-page":"1786","article-title":"Role of extracellular proteases in biofilm degradation produced from Escherichia coli and Pseudomonas mendocina","volume":"7","author":"Kumari","year":"2018","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"10.1016\/j.micpath.2020.104555_bib15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1111\/1574-6968.12114","article-title":"Effect of proteases on biofilm formation of the plastic-degrading actinomycete Rhodococcus ruber C208","volume":"342","author":"Gilan","year":"2013","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.micpath.2020.104555_bib16","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.micinf.2009.10.005","article-title":"Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus","volume":"12","author":"Mart\u00ed","year":"2010","journal-title":"Microbes. infect."},{"key":"10.1016\/j.micpath.2020.104555_bib17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3390\/md18010033","article-title":"Halophiles and their biomolecules: recent advances and future applications in biomedicine","volume":"18","author":"Corral","year":"2020","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.micpath.2020.104555_bib18","doi-asserted-by":"crossref","first-page":"3275","DOI":"10.1007\/s11033-019-04787-7","article-title":"Isolation and screening of extracellular anticancer enzymes from halophilic and halotolerant bacteria from different saline environments in Iran","volume":"46","author":"Zolfaghar","year":"2019","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.micpath.2020.104555_bib19","first-page":"492","article-title":"Halobacillus gen. nov., with Descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and Transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov","volume":"46","author":"Spring","year":"1996","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib20","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1099\/ijs.0.02448-0","article-title":"Halobacillus karajensis sp. nov., a novel moderate halophile","volume":"53","author":"Amoozegar","year":"2003","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib21","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1002\/jobm.201600391","article-title":"Characterization of thermo\u2010solvent stable protease from Halobacillus sp. CJ4 isolated from Chott Eldjerid hypersaline lake in Tunisia","volume":"57","author":"Daoud","year":"2017","journal-title":"J. Basic Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib22","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1271\/bbb.50658","article-title":"A halophilic serine proteinase from Halobacillus sp. SR5-3 isolated from fish sauce: purification and characterization","volume":"70","author":"Namwong","year":"2006","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"10.1016\/j.micpath.2020.104555_bib23","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1590\/S1517-83822014005000015","article-title":"Extracellular proteases of Halobacillus blutaparonensis strain M9, a new moderately halophilic bacterium","volume":"44","author":"Santos","year":"2013","journal-title":"Braz. J. Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib24","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10295-008-0466-y","article-title":"Production, optimization and purification of a novel extracellular protease from the moderately halophilic bacterium Halobacillus karajensis","volume":"36","author":"Karbalaei-Heidari","year":"2009","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"10.1016\/j.micpath.2020.104555_bib25","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/S0167-7012(02)00191-4","article-title":"Production of amylase by newly isolated moderate halophile, Halobacillus sp. strain MA-2","volume":"52","author":"Amoozegar","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.micpath.2020.104555_bib26","series-title":"Handbook of Hydrocarbon and Lipid Microbiology","first-page":"3688","article-title":"Isolation and analysis of lipopeptides and high molecular weight biosurfactants","author":"Smyth","year":"2010"},{"key":"10.1016\/j.micpath.2020.104555_bib27","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1128\/iai.22.3.842-851.1978","article-title":"Extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilute acid","volume":"22","author":"Campbell","year":"1978","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2020.104555_bib28","first-page":"267","article-title":"On the structural transformations and lysis of halobacterium salinarium in hypotonic and isotonic solutions","volume":"31","author":"Mohr","year":"1963","journal-title":"Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib29","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.saa.2015.03.104","article-title":"Identification of different species of Bacillus isolated from nisargruna biogas plant by FTIR, UV\u2013vis and NIR spectroscopy","volume":"148","author":"Ghosh","year":"2015","journal-title":"Spectrochim. Acta Mol. Biomol. Spectrosc."},{"key":"10.1016\/j.micpath.2020.104555_bib30","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.colsurfb.2008.02.018","article-title":"Biosynthesis of silver nanocrystals by Bacillus licheniformis","volume":"65","author":"Kalimuthu","year":"2008","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.micpath.2020.104555_bib31","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ibiod.2006.09.006","article-title":"Identification of low molecular weight aromatic compounds by gas chromatography\u2013mass spectrometry (GC\u2013MS) from kraft lignin degradation by three Bacillus sp","volume":"59","author":"Raj","year":"2007","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"10.1016\/j.micpath.2020.104555_bib32","first-page":"28","article-title":"Toxicity and unusual biological behavior of nanosilver on gram positive and negative bacteria assayed by microtiter-plate","volume":"1","author":"Shahrokh","year":"2009","journal-title":"Eur. J. Biol. Sci."},{"key":"10.1016\/j.micpath.2020.104555_bib33","doi-asserted-by":"crossref","DOI":"10.1063\/1.5141438","article-title":"Spectroscopic characteristics and energy transfer of bacterial pigment:(pyocyanin\/curcumin)","volume":"2201","author":"Wadday","year":"2019","journal-title":"AIP Conference Proceedings"},{"key":"10.1016\/j.micpath.2020.104555_bib34","first-page":"1101","article-title":"Analysis of pyocyanin compound and its antagonistic activity against phytopathogens","volume":"5","author":"Sudhakar","year":"2013","journal-title":"Int. .J Chem. Tech. Res."},{"key":"10.1016\/j.micpath.2020.104555_bib35","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.molliq.2018.07.103","article-title":"Microbial assisted (Bacillus mojavensis) production of bio-surfactant lipopeptide with potential pharmaceutical applications and its characterization by MALDI-TOF-MS analysis","volume":"268","author":"Fanaei","year":"2018","journal-title":"J. Mol. Liq."},{"key":"10.1016\/j.micpath.2020.104555_bib36","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s13213-011-0359-6","article-title":"Co-production of a uv-stable bacteriocin-like inhibitory substance (BLIS) and indole-3-acetic acid hormone (IAA) and their optimization by Taguchi design in Bacillus pumilus","volume":"62","author":"Zaghian","year":"2012","journal-title":"Ann. Microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib37","series-title":"Purification and Properties of Lysozyme from Pseudomonas aeruginosa Bacteriophage 7v","author":"McFarland","year":"1977"},{"key":"10.1016\/j.micpath.2020.104555_bib38","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0178943","article-title":"Differential stability of therapeutic peptides with different proteolytic cleavage sites in blood, plasma and serum","volume":"12","author":"B\u00f6ttger","year":"2017","journal-title":"PloS One"},{"key":"10.1016\/j.micpath.2020.104555_bib39","first-page":"813","article-title":"Role of fatty acids in bacillus environmental adaptation","volume":"6","author":"Diomande","year":"2015","journal-title":"front. In. microbiol."},{"key":"10.1016\/j.micpath.2020.104555_bib40","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s40643-014-0008-6","article-title":"Purification and partial characterization of serine alkaline metalloprotease from Bacillus brevis MWB-01","volume":"1","author":"Olajuyigbe","year":"2014","journal-title":"bioresour. bioprocess."},{"key":"10.1016\/j.micpath.2020.104555_bib41","doi-asserted-by":"crossref","first-page":"2553","DOI":"10.1016\/S0968-0896(02)00108-6","article-title":"Cleavage of \u03b2-lactone ring by serine protease. Mechanistic implications","volume":"10","author":"Kim","year":"2002","journal-title":"Bioorganic. med. chem."},{"key":"10.1016\/j.micpath.2020.104555_bib42","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00543-18","article-title":"A biofilm matrix-associated protease inhibitor protects Pseudomonas aeruginosa from proteolytic attack","volume":"9","author":"Tseng","year":"2018","journal-title":"mBio"},{"key":"10.1016\/j.micpath.2020.104555_bib43","doi-asserted-by":"crossref","DOI":"10.1002\/mbo3.635","article-title":"Exogenous fatty acids alter phospholipid composition, membrane permeability, capacity for biofilm formation, and antimicrobial peptide susceptibility in Klebsiella pneumoniae","volume":"8","author":"Hobby","year":"2019","journal-title":"Microbiol. Open ."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020309219?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020309219?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:41:32Z","timestamp":1760110892000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401020309219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":43,"alternative-id":["S0882401020309219"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104555","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Inhibition of quorum sensing-controlled virulence factors with natural substances and novel protease, obtained from Halobacillus karajensis","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104555","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104555"}}