{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:43:45Z","timestamp":1791863025432,"version":"4.3.4"},"reference-count":53,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["PPP045\u20132013B"],"award-info":[{"award-number":["PPP045\u20132013B"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002385","name":"Ministry of Higher Education","doi-asserted-by":"publisher","award":["PPP045\u20132013B"],"award-info":[{"award-number":["PPP045\u20132013B"]}],"id":[{"id":"10.13039\/501100002385","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["PPP045\u20132013B"],"award-info":[{"award-number":["PPP045\u20132013B"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2021,3]]},"DOI":"10.1016\/j.micpath.2020.104614","type":"journal-article","created":{"date-parts":[[2020,11,14]],"date-time":"2020-11-14T09:00:03Z","timestamp":1605344403000},"page":"104614","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":18,"special_numbering":"C","title":["LC-MS analysis reveals biological and metabolic processes essential for Candida albicans biofilm growth"],"prefix":"10.1016","volume":"152","author":[{"given":"Komathy","family":"Munusamy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mun Fai","family":"Loke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamuna","family":"Vadivelu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3120-6998","authenticated-orcid":false,"given":"Sun Tee","family":"Tay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2020.104614_bib1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev-micro-091014-104330","article-title":"Candida albicans biofilms and human disease","volume":"69","author":"Nobile","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.diagmicrobio.2012.02.001","article-title":"The changing epidemiology of healthcare-associated candidemia over three decades","volume":"73","author":"Diekema","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"10.1016\/j.micpath.2020.104614_bib3","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/civ933","article-title":"Clinical practice guideline for the management of candidiasis: 2016 update by the Infectious Diseases Society of America","volume":"62","author":"Pappas","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.micpath.2020.104614_bib4","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.2217\/fmb.13.101","article-title":"Mechanisms of Candida biofilm drug resistance","volume":"8","author":"Taff","year":"2013","journal-title":"Future Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib5","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0033705","article-title":"Risk factors and outcomes of candidemia caused by biofilm-forming isolates in a tertiary care hospital","volume":"7","author":"Tumbarello","year":"2012","journal-title":"PloS One"},{"key":"10.1016\/j.micpath.2020.104614_bib6","series-title":"Advances in Applied Microbiology","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/B978-0-12-407673-0.00002-3","article-title":"Chapter Two - The clinical importance of fungal biofilms","author":"Ramage","year":"2013"},{"key":"10.1016\/j.micpath.2020.104614_bib7","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s11046-013-9616-5","article-title":"Candidemia by species of the Candida parapsilosis complex in children's hospital: prevalence, biofilm production and antifungal susceptibility","volume":"175","author":"Ruiz","year":"2013","journal-title":"Mycopathologia"},{"key":"10.1016\/j.micpath.2020.104614_bib8","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1128\/MMBR.64.4.847-867.2000","article-title":"Microbial biofilms: from ecology to molecular genetics","volume":"64","author":"Davey","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.micpath.2020.104614_bib9","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S0966-842X(02)00002-1","article-title":"Candida biofilms and their role in infection","volume":"11","author":"Douglas","year":"2003","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib10","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.tim.2014.02.001","article-title":"Molecular mechanisms of antimicrobial tolerance and resistance in bacterial and fungal biofilms","volume":"22","author":"Van Acker","year":"2014","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.01333-14","article-title":"Novel entries in a fungal biofilm matrix Encyclopedia","volume":"5","author":"Zarnowski","year":"2014","journal-title":"mBio"},{"key":"10.1016\/j.micpath.2020.104614_bib12","doi-asserted-by":"crossref","first-page":"2128 LP","DOI":"10.1128\/AAC.39.9.2128","article-title":"Resistance of Candida albicans biofilms to antifungal agents in vitro","volume":"39","author":"Hawser","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.micpath.2020.104614_bib13","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1128\/IAI.71.8.4333-4340.2003","article-title":"Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of efflux pumps and membrane sterols","volume":"71","author":"Mukherjee","year":"2003","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2020.104614_bib14","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1111\/j.1601-0825.2007.01424.x","article-title":"Biofilm lifestyle of Candida: a mini review","volume":"14","author":"Seneviratne","year":"2008","journal-title":"Oral Dis."},{"key":"10.1016\/j.micpath.2020.104614_bib15","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1128\/EC.3.2.536-545.2004","article-title":"Candida albicans biofilms: a developmental state associated with specific and stable gene expression patterns","volume":"3","author":"Garc\u00eda-S\u00e1nchez","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib16","doi-asserted-by":"crossref","first-page":"1562 LP","DOI":"10.1128\/EC.4.9.1562-1573.2005","article-title":"Genome-wide transcription profiling of the early phase of biofilm formation by Candida albicans","volume":"4","author":"Murillo","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib17","doi-asserted-by":"crossref","first-page":"3452","DOI":"10.1091\/mbc.e02-05-0272","article-title":"Transcription profiling of Candida albicans cells undergoing the yeast-to-hyphal transition","volume":"13","author":"Nantel","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib18","doi-asserted-by":"crossref","first-page":"5795","DOI":"10.1002\/pmic.200600332","article-title":"Proteomics for the analysis of the Candida albicans biofilm lifestyle","volume":"6","author":"Thomas","year":"2006","journal-title":"Proteomics"},{"key":"10.1016\/j.micpath.2020.104614_bib19","doi-asserted-by":"crossref","first-page":"2373","DOI":"10.1099\/mic.0.2007\/006163-0","article-title":"Temporal analysis of Candida albicans gene expression during biofilm development","volume":"153","author":"Yeater","year":"2007","journal-title":"Microbiology"},{"key":"10.1016\/j.micpath.2020.104614_bib20","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.1002\/pmic.200700594","article-title":"Proteomic analysis of cytoplasmic and surface proteins from yeast cells, hyphae, and biofilms of Candida albicans","volume":"9","author":"Mart\u00ednez-Gomariz","year":"2009","journal-title":"Proteomics"},{"key":"10.1016\/j.micpath.2020.104614_bib21","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1128\/AAC.00756-09","article-title":"Changes in the proteome of Candida albicans in response to azole, polyene, and echinocandin antifungal agents","volume":"54","author":"Hoehamer","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.micpath.2020.104614_bib22","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1021\/acs.jproteome.5b00913","article-title":"Apoptosis of Candida albicans during the interaction with murine macrophages: proteomics and cell-death marker monitoring","volume":"15","author":"Cabez\u00f3n","year":"2016","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.micpath.2020.104614_bib23","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/mas.20128","article-title":"Analytical strategies for identifying drug metabolites","volume":"26","author":"Prakash","year":"2007","journal-title":"Mass Spectrom. Rev."},{"key":"10.1016\/j.micpath.2020.104614_bib24","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.jprot.2015.06.006","article-title":"Candida albicans cell shaving uncovers new proteins involved in cell wall integrity, yeast to hypha transition, stress response and host-pathogen interaction","volume":"127","author":"Gil-Bona","year":"2015","journal-title":"J. Proteomics."},{"key":"10.1016\/j.micpath.2020.104614_bib25","doi-asserted-by":"crossref","first-page":"D592","DOI":"10.1093\/nar\/gkw924","article-title":"The Candida Genome Database (CGD): incorporation of Assembly 22, systematic identifiers and visualization of high throughput sequencing data","volume":"45","author":"Skrzypek","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2020.104614_bib26","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gkv1145","article-title":"2016 update of the PRIDE database and its related tools","volume":"44","author":"Vizca\u00edno","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2020.104614_bib27","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1128\/EC.00295-09","article-title":"Candida albicans PEP12 is required for biofilm integrity and in vivo virulence","volume":"9","author":"Palanisamy","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib28","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s00294-010-0323-1","article-title":"The Candida albicans Kar2 protein is essential and functions during the translocation of proteins into the endoplasmic reticulum","volume":"57","author":"Morrow","year":"2011","journal-title":"Curr. Genet."},{"key":"10.1016\/j.micpath.2020.104614_bib29","doi-asserted-by":"crossref","first-page":"4751","DOI":"10.1242\/jcs.02599","article-title":"Yeast Mon2p is a highly conserved protein that functions in the cytoplasm-to-vacuole transport pathway and is required for Golgi homeostasis","volume":"118","author":"Efe","year":"2005","journal-title":"J. Cell Sci."},{"key":"10.1016\/j.micpath.2020.104614_bib30","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.3389\/fmicb.2019.01012","article-title":"Candida albicans Pma1p contributes to growth, pH homeostasis, and hyphal formation","volume":"10","author":"Rane","year":"2019","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib31","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1749-6632.1997.tb52330.x","article-title":"The plasma membrane H(+)-ATPase of fungi. A candidate drug target?","volume":"834","author":"Perlin","year":"1997","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.micpath.2020.104614_bib32","doi-asserted-by":"crossref","first-page":"fov027","DOI":"10.1093\/femsyr\/fov027","article-title":"System-level impact of mitochondria on fungal virulence: to metabolism and beyond","volume":"15","author":"Calderone","year":"2015","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.micpath.2020.104614_bib33","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3389\/fcimb.2017.00520","article-title":"Candida albicans heat shock proteins and hsps-associated signaling pathways as potential antifungal targets","volume":"7","author":"Gong","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib34","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1016\/S1097-2765(01)00270-2","article-title":"A DNA damage response pathway controlled by Tel1 and the Mre11 complex","volume":"7","author":"Usui","year":"2001","journal-title":"Mol. Cell."},{"key":"10.1016\/j.micpath.2020.104614_bib35","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1128\/iai.64.8.3333-3340.1996","article-title":"Evidence for presence in the cell wall of Candida albicans of a protein related to the hsp70 family","volume":"64","author":"L\u00f3pez-Ribot","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1016\/j.micpath.2020.104614_bib36","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s12275-013-2577-z","article-title":"Candida albicans ENO1 null mutants exhibit altered drug susceptibility, hyphal formation, and virulence","volume":"51","author":"Ko","year":"2013","journal-title":"J. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib37","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1128\/EC.00115-06","article-title":"Effects of depleting the essential central metabolic enzyme fructose-1,6-bisphosphate aldolase on the growth and viability of Candida albicans: implications for antifungal drug target discovery","volume":"5","author":"Rodaki","year":"2006","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib38","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1016\/j.febslet.2014.02.042","article-title":"NAD(+)-linked alcohol dehydrogenase 1 regulates methylglyoxal concentration in Candida albicans","volume":"588","author":"Kwak","year":"2014","journal-title":"FEBS Lett."},{"key":"10.1016\/j.micpath.2020.104614_bib39","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.3389\/fmicb.2018.01025","article-title":"The F(1)F(o)-ATP synthase \u03b2 subunit is required for Candida albicans pathogenicity due to its role in carbon flexibility","volume":"9","author":"Li","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib40","doi-asserted-by":"crossref","first-page":"35436","DOI":"10.1038\/srep35436","article-title":"Integrating Candida albicans metabolism with biofilm heterogeneity by transcriptome mapping","volume":"6","author":"Rajendran","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2020.104614_bib41","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.3390\/s120302519","article-title":"Quorum sensing and bacterial social interactions in biofilms","volume":"12","author":"Li","year":"2012","journal-title":"Sensors (Basel)."},{"key":"10.1016\/j.micpath.2020.104614_bib42","doi-asserted-by":"crossref","first-page":"7263","DOI":"10.1007\/s00253-016-7532-6","article-title":"Establishment of a multi-species biofilm model and metatranscriptomic analysis of biofilm and planktonic cell communities","volume":"100","author":"Nakamura","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.micpath.2020.104614_bib43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0072968","article-title":"Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells","volume":"8","author":"Rumbo-Feal","year":"2013","journal-title":"PloS One"},{"key":"10.1016\/j.micpath.2020.104614_bib44","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1128\/EC.2.5.1018-1024.2003","article-title":"Candida albicans response regulator gene SSK1 regulates a subset of genes whose functions are associated with cell wall biosynthesis and adaptation to oxidative stress","volume":"2","author":"Chauhan","year":"2003","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib45","doi-asserted-by":"crossref","DOI":"10.1128\/MMBR.68.3.501-517.2004","article-title":"Microbial type I fatty acid synthases (FAS): major players in a network of cellular FAS systems","volume":"68","author":"Schweizer","year":"2004","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.micpath.2020.104614_bib46","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1128\/EC.00127-13","article-title":"Impact of environmental conditions on the form and function of Candida albicans biofilms","volume":"12","author":"Daniels","year":"2013","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib47","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1128\/EC.3.6.1574-1588.2004","article-title":"Deletion of the dynein heavy-chain gene DYN1 leads to aberrant nuclear positioning and defective hyphal development in Candida albicans","volume":"3","author":"Martin","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib48","doi-asserted-by":"crossref","first-page":"2733 LP","DOI":"10.1128\/AAC.48.7.2733-2735.2004","article-title":"Proteomic analysis of azole resistance in Candida albicans clinical isolates","volume":"48","author":"Hooshdaran","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"10.1016\/j.micpath.2020.104614_bib49","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1002\/yea.320100609","article-title":"Comparison of INO1 gene sequences and products in Candida albicans and Saccharomyces cerevisiae","volume":"10","author":"Klig","year":"1994","journal-title":"Yeast"},{"key":"10.1016\/j.micpath.2020.104614_bib50","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/S1369-5274(02)00338-7","article-title":"Fungal morphogenesis and host invasion","volume":"5","author":"Gow","year":"2002","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.micpath.2020.104614_bib51","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1128\/EC.2.5.1053-1060.2003","article-title":"Engineered control of cell morphology in vivo reveals distinct roles for yeast and filamentous forms of Candida albicans during infection","volume":"2","author":"Saville","year":"2003","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.micpath.2020.104614_bib52","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1242\/jeb.088930","article-title":"Stress adaptation in a pathogenic fungus","volume":"217","author":"Brown","year":"2014","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.micpath.2020.104614_bib53","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.mib.2009.05.006","article-title":"How human pathogenic fungi sense and adapt to pH: the link to virulence","volume":"12","author":"Davis","year":"2009","journal-title":"Curr. Opin. Microbiol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020309803?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401020309803?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:36:41Z","timestamp":1760110601000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401020309803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3]]},"references-count":53,"alternative-id":["S0882401020309803"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104614","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"LC-MS analysis reveals biological and metabolic processes essential for Candida albicans biofilm growth","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2020.104614","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"104614"}}