{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T23:55:14Z","timestamp":1785110114766,"version":"3.55.0"},"reference-count":58,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100006402","name":"Fasa University of Medical Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Microbial Pathogenesis"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1016\/j.micpath.2021.104970","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T12:11:09Z","timestamp":1621426269000},"page":"104970","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":32,"special_numbering":"C","title":["Design, expression, and purification of a multi-epitope vaccine against Helicobacter Pylori based on Melittin as an adjuvant"],"prefix":"10.1016","volume":"157","author":[{"given":"Elham","family":"Jafari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirin","family":"Mahmoodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.micpath.2021.104970_bib1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.12980\/jclm.5.2017J6-238","article-title":"Co\u2010Infection of Helicobacter pylori and Escherichia coli in a 4\u2010year\u2010old child","volume":"5","author":"Heidarzadeh","year":"2017","journal-title":"J.Coast. Life Med."},{"key":"10.1016\/j.micpath.2021.104970_bib2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.arcmed.2019.11.001","article-title":"Helicobacter heilmannii colonization is associated with high risk for gastritis","volume":"50","author":"Mohammadi","year":"2019","journal-title":"Arch. Med. Res."},{"key":"10.1016\/j.micpath.2021.104970_bib3","doi-asserted-by":"crossref","first-page":"5578","DOI":"10.3748\/wjg.v25.i37.5578","article-title":"Pathogenesis and clinical management of Helicobacter pylori gastric infection","volume":"25","author":"de Brito","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"10.1016\/j.micpath.2021.104970_bib4","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/5584_2019_361","article-title":"Helicobacter pylori infection in children and adolescents","volume":"1149","author":"Okuda","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.micpath.2021.104970_bib5","first-page":"1","article-title":"Helicobacter pylori in developing countries","author":"Hunt","year":"2010","journal-title":"World . Gastroenterol.Organisat.Global Guidelines"},{"key":"10.1016\/j.micpath.2021.104970_bib6","doi-asserted-by":"crossref","first-page":"14800","DOI":"10.1002\/jcp.28279","article-title":"Current information on the association of Helicobacter pylori with autophagy and gastric cancer","volume":"234","author":"Eslami","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.micpath.2021.104970_bib7","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1111\/1462-2920.13222","article-title":"How Helicobacter pylori senses, targets and interacts with the gastric epithelium","volume":"18","author":"Keilberg","year":"2016","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.micpath.2021.104970_bib8","series-title":"Update on the Management of Helicobacter Pylori Infection. Current Treatment Options in Gastroenterology","first-page":"1","author":"Saleem","year":"2020"},{"key":"10.1016\/j.micpath.2021.104970_bib9","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/j.meegid.2017.08.002","article-title":"A novel hpv prophylactic peptide vaccine, designed by immunoinformatics and structural vaccinology approaches","volume":"54","author":"Negahdaripour","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.micpath.2021.104970_bib10","doi-asserted-by":"crossref","first-page":"51","DOI":"10.4110\/in.2015.15.2.51","article-title":"Recent advances of vaccine adjuvants for infectious diseases","volume":"15","author":"Lee","year":"2015","journal-title":"Immune network"},{"key":"10.1016\/j.micpath.2021.104970_bib11","doi-asserted-by":"crossref","first-page":"128","DOI":"10.3390\/vaccines8010128","article-title":"Better adjuvants for better vaccines: progress in adjuvant delivery systems, modifications, and adjuvant\u2013antigen codelivery","volume":"8","author":"Wang","year":"2020","journal-title":"Vaccines"},{"key":"10.1016\/j.micpath.2021.104970_bib12","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3389\/fimmu.2020.00442","article-title":"Better epitope discovery, precision immune engineering, and accelerated vaccine design using immunoinformatics tools","volume":"11","author":"De Groot","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.micpath.2021.104970_bib13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.apcsb.2015.03.001","article-title":"Peptide immunotherapy in vaccine development: from epitope to adjuvant","volume":"99","author":"Yang","year":"2015","journal-title":"Adv. Protein. Chem.Struct. Biol."},{"key":"10.1016\/j.micpath.2021.104970_bib14","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1080\/08830185.2019.1657426","article-title":"Immunoinformatics: in silico approaches and computational design of a multi-epitope, immunogenic protein","volume":"38","author":"Bahrami","year":"2019","journal-title":"Int. Rev. Immunol."},{"key":"10.1016\/j.micpath.2021.104970_bib15","doi-asserted-by":"crossref","DOI":"10.1111\/hel.12517","article-title":"Helicobacter: inflammation, immunology, and vaccines","volume":"23","author":"Blosse","year":"2018","journal-title":"Helicobacter"},{"key":"10.1016\/j.micpath.2021.104970_bib16","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s10120-018-0867-1","article-title":"Pathogenicity of Helicobacter pylori in cancer development and impacts of vaccination","volume":"22","author":"Maleki Kakelar","year":"2019","journal-title":"Gastric Cancer : Off. J. Int.Gastric Cancer.Assoc. Jpn.Gastric Cancer.Assoc"},{"key":"10.1016\/j.micpath.2021.104970_bib17","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1146\/annurev.immunol.19.1.523","article-title":"The design of vaccines against Helicobacter pylori and their development","volume":"19","author":"Del Giudice","year":"2001","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.micpath.2021.104970_bib18","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1016\/j.vaccine.2010.11.088","article-title":"A truncated form of hpaa is a promising antigen for use in a vaccine against Helicobacter pylori","volume":"29","author":"Flach","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.micpath.2021.104970_bib19","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/41483","article-title":"The complete genome sequence of the gastric pathogen Helicobacter pylori","volume":"388","author":"Tomb","year":"1997","journal-title":"Nature"},{"key":"10.1016\/j.micpath.2021.104970_bib20","doi-asserted-by":"crossref","first-page":"421","DOI":"10.3748\/wjg.v11.i3.421","article-title":"Frequencies of the expression of main protein antigens from Helicobacter pylori isolates and production of specific serum antibodies in infected patients","volume":"11","author":"Yan","year":"2005","journal-title":"World J. Gastroenterol.: WJG"},{"key":"10.1016\/j.micpath.2021.104970_bib21","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.freeradbiomed.2016.09.024","article-title":"Polyamine-and Nadph-dependent generation of Ros during Helicobacter pylori infection: a blessing in disguise","volume":"105","author":"Gobert","year":"2017","journal-title":"Free Radic. Biol. Med."},{"key":"10.1016\/j.micpath.2021.104970_bib22","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1038\/mt.2013.153","article-title":"An infection-enhanced oncolytic adenovirus secreting H. Pylori Neutrophil-activating protein with therapeutic effects on Neuroendocrine tumors","volume":"21","author":"Ramachandran","year":"2013","journal-title":"Mol. Ther. : J.Am. Soc.Gene Therapy"},{"key":"10.1016\/j.micpath.2021.104970_bib23","doi-asserted-by":"crossref","first-page":"320","DOI":"10.3390\/vaccines3020320","article-title":"Vaccine adjuvants: from 1920 to 2015 and beyond","volume":"3","author":"Pasquale","year":"2015","journal-title":"Vaccines"},{"key":"10.1016\/j.micpath.2021.104970_bib24","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1186\/s12967-016-0910-0","article-title":"Melittin-Mil-2 fusion protein as a candidate for cancer immunotherapy","volume":"14","author":"Liu","year":"2016","journal-title":"J. Transl. Med."},{"key":"10.1016\/j.micpath.2021.104970_bib25","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1053\/j.gastro.2019.09.002","article-title":"Suppressive and gut-Reparative functions of human type 1 T regulatory cells","volume":"157","author":"Cook","year":"2019","journal-title":"Gastroenterology"},{"key":"10.1016\/j.micpath.2021.104970_bib26","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.4049\/jimmunol.142.9.3129","article-title":"Il-1 as adjuvant. Role of T Cells in the augmentation of specific antibody production by Recombinant human il-1 alpha","volume":"142","author":"Reed","year":"1989","journal-title":"J. Immunol."},{"key":"10.1016\/j.micpath.2021.104970_bib27","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0169-409X(01)00166-1","article-title":"Intranasal vaccination against plague, tetanus and diphtheria","volume":"51","author":"Alpar","year":"2001","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.micpath.2021.104970_bib28","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1080\/10611860410001670080","article-title":"Adjuvant action of Melittin following intranasal immunisation with tetanus and diphtheria toxoids","volume":"11","author":"Bramwell","year":"2003","journal-title":"J. Drug Target."},{"key":"10.1016\/j.micpath.2021.104970_bib29","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1080\/1040841X.2017.1291578","article-title":"The study of H. Pylori putative candidate factors for single- and multi-component vaccine development","volume":"43","author":"Mirzaei","year":"2017","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.micpath.2021.104970_bib30","series-title":"Predicting Secretory Proteins with Signalp. Protein Function Prediction","first-page":"59","author":"Nielsen","year":"2017"},{"key":"10.1016\/j.micpath.2021.104970_bib31","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1471-2105-11-41","article-title":"Prediction of the binding affinities of peptides to class ii mhc using a Regularized thermodynamic model","volume":"11","author":"Bordner","year":"2010","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.micpath.2021.104970_bib32","series-title":"Immunoinformatics: Predicting Immunogenicity in Silico","author":"Flower","year":"2007"},{"key":"10.1016\/j.micpath.2021.104970_bib33","doi-asserted-by":"crossref","first-page":"3621","DOI":"10.1093\/nar\/gkg510","article-title":"Mhcpred: a server for quantitative prediction of peptide\u2013mhc binding","volume":"31","author":"Guan","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2021.104970_bib34","doi-asserted-by":"crossref","first-page":"W513","DOI":"10.1093\/nar\/gkn254","article-title":"Immune epitope database analysis Resource (iedb-Ar)","volume":"36","author":"Zhang","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2021.104970_bib35","doi-asserted-by":"crossref","DOI":"10.1098\/rsob.120139","article-title":"T-cell epitope vaccine design by immunoinformatics","volume":"3","author":"Patronov","year":"2013","journal-title":"Open biology"},{"key":"10.1016\/j.micpath.2021.104970_bib36","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1745-6150-8-30","article-title":"Designing of interferon-gamma inducing mhc class-ii binders","volume":"8","author":"Dhanda","year":"2013","journal-title":"Biol. Direct"},{"key":"10.1016\/j.micpath.2021.104970_bib37","doi-asserted-by":"crossref","first-page":"W9","DOI":"10.1093\/nar\/gkx346","article-title":"Bepipred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"Jespersen","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2021.104970_bib38","series-title":"Bcepred: Prediction of Continuous B-Cell Epitopes in Antigenic Sequences Using Physico-Chemical Properties","first-page":"197","author":"Saha","year":"2004"},{"key":"10.1016\/j.micpath.2021.104970_bib39","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1002\/prot.21078","article-title":"Prediction of continuous B\u2010cell epitopes in an antigen using Recurrent neural network","volume":"65","author":"Saha","year":"2006","journal-title":"Proteins: Structure, Function, and Bioinformatics"},{"key":"10.1016\/j.micpath.2021.104970_bib40","doi-asserted-by":"crossref","first-page":"2936","DOI":"10.1093\/bioinformatics\/btq551","article-title":"High-throughput prediction of protein antigenicity using protein microarray data","volume":"26","author":"Magnan","year":"2010","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2021.104970_bib41","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1471-2105-8-4","article-title":"Vaxijen: a server for prediction of protective antigens, tumour antigens and subunit vaccines","volume":"8","author":"Doytchinova","year":"2007","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.micpath.2021.104970_bib42","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1007\/s00894-014-2278-5","article-title":"Allertop V. 2\u2014a server for in silico prediction of allergens","volume":"20","author":"Dimitrov","year":"2014","journal-title":"J. Mol. Model."},{"key":"10.1016\/j.micpath.2021.104970_bib43","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1093\/bioinformatics\/btt619","article-title":"Allergenfp: allergenicity prediction by descriptor fingerprints","volume":"30","author":"Dimitrov","year":"2014","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2021.104970_bib44","doi-asserted-by":"crossref","first-page":"W9","DOI":"10.1093\/nar\/gkl343","article-title":"Algpred: prediction of allergenic proteins and mapping of ige epitopes","volume":"34","author":"Saha","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2021.104970_bib45","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1093\/bioinformatics\/btp386","article-title":"Solpro: accurate sequence-based prediction of protein solubility","volume":"25","author":"Magnan","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.micpath.2021.104970_bib46","series-title":"Protein Identification and Analysis Tools on the Expasy Server","first-page":"571","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.micpath.2021.104970_bib47","doi-asserted-by":"crossref","first-page":"W526","DOI":"10.1093\/nar\/gki376","article-title":"Jcat: a novel tool to adapt codon usage of a target gene to its potential expression host","volume":"33","author":"Grote","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.micpath.2021.104970_bib48","series-title":"Preparation and Extraction of Insoluble (Inclusion-Body) Proteins from Escherichia Coli","author":"Palmer","year":"2004"},{"key":"10.1016\/j.micpath.2021.104970_bib49","doi-asserted-by":"crossref","first-page":"10.7. 1","DOI":"10.1002\/0471140864.ps1007s82","article-title":"Electroblotting from polyacrylamide gels","volume":"82","author":"Goldman","year":"2015","journal-title":"Current protocols in protein science"},{"key":"10.1016\/j.micpath.2021.104970_bib50","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/S0140-6736(15)60310-5","article-title":"Efficacy, safety, and immunogenicity of an oral Recombinant Helicobacter pylori vaccine in children in China: a Randomised, double-blind, placebo-controlled, Phase 3 trial","volume":"386","author":"Zeng","year":"2015","journal-title":"Lancet"},{"key":"10.1016\/j.micpath.2021.104970_bib51","doi-asserted-by":"crossref","first-page":"12050","DOI":"10.18632\/oncotarget.23927","article-title":"Immunodominant antigens that induce Th1 and Th17 responses protect mice against Helicobacter pylori infection","volume":"9","author":"Sun","year":"2018","journal-title":"Oncotarget"},{"key":"10.1016\/j.micpath.2021.104970_bib52","doi-asserted-by":"crossref","first-page":"72","DOI":"10.3390\/vaccines6040072","article-title":"Effect of Melittin on metabolomic profile and cytokine production in pma-differentiated thp-1 cells","volume":"6","author":"Alqarni","year":"2018","journal-title":"Vaccines"},{"key":"10.1016\/j.micpath.2021.104970_bib53","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0304-4165(93)90077-L","article-title":"The effect of sequence variations and structure on the cytolytic activity of Melittin peptides","volume":"1157","author":"Werkmeister","year":"1993","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.micpath.2021.104970_bib54","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.addr.2012.09.039","article-title":"Fusion protein linkers: property, design and functionality","volume":"65","author":"Chen","year":"2013","journal-title":"Adv. Drug Deliv. Rev."},{"key":"10.1016\/j.micpath.2021.104970_bib55","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.meegid.2017.04.009","article-title":"Development of a multi-epitope peptide vaccine inducing Robust T cell responses against brucellosis using immunoinformatics based approaches","volume":"51","author":"Saadi","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.micpath.2021.104970_bib56","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1089\/cmb.2019.0062","article-title":"Immunoinformatics approach to design a novel epitope-based oral vaccine against Helicobacter pylori","volume":"26","author":"Urrutia-Baca","year":"2019","journal-title":"J. Comput. Biol."},{"key":"10.1016\/j.micpath.2021.104970_bib57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-49354-z","article-title":"Immunoinformatics approaches to explore Helicobacter pylori proteome (virulence factors) to design B and T cell multi-epitope subunit vaccine","volume":"9","author":"Khan","year":"2019","journal-title":"Sci. Rep."},{"key":"10.1016\/j.micpath.2021.104970_bib58","doi-asserted-by":"crossref","first-page":"349","DOI":"10.3389\/fcimb.2017.00349","article-title":"Oral immunization with a multivalent epitope-based vaccine, based on Nap, urease, Hsp60, and hpaa, provides therapeutic effect on H. Pylori infection in Mongolian gerbils","volume":"7","author":"Guo","year":"2017","journal-title":"Front.Cell. Infect. Microbiol."}],"container-title":["Microbial Pathogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401021002424?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0882401021002424?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:43:09Z","timestamp":1760110989000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0882401021002424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":58,"alternative-id":["S0882401021002424"],"URL":"https:\/\/doi.org\/10.1016\/j.micpath.2021.104970","relation":{},"ISSN":["0882-4010"],"issn-type":[{"value":"0882-4010","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Design, expression, and purification of a multi-epitope vaccine against Helicobacter Pylori based on Melittin as an adjuvant","name":"articletitle","label":"Article Title"},{"value":"Microbial Pathogenesis","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.micpath.2021.104970","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"104970"}}